Biomarkers of early vascular endothelial injury with low-arsenic exposure in drinking water

Q3 Medicine

中华地方病学杂志 Pub Date : 2011-09-20 DOI:10.3760/CMA.J.ISSN.1000-4955.2011.05.006

Chun-yan Ji, Chun Fu, Q. Xiang, Song Xu, Mingsheng Zhu, Jian Liu, Dapeng Wang, Jie Zhang, Yan An

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引用次数: 1

Abstract

Objective To observes the change of early effective biomarkers of endothelial injury with lowarsenic exposure in drinking water. Methods Ninety rurad residents, who had lived in Yanhe village, Xuyi county and Jiangsu province for at least 10 years, were recruited by simple random sampling in this study. The level of arsenic in their household shallow well were divided into three groups, which were ＜ 10 (32 people), 10 - 50(28 people) and ＞ 50 μg/L(30 people). Blood samples from individuals were collected. Malondialdehyde(MDA) in human plasma, which is considered as the most important marker for monitoring lipid peroxidation, was determined as conjugate with tetrabutylammonium hydrogen sulphate(TBA). The level of anti-superoxide anion radical(O-·2),C-reactive protein(CRP) and NO in human plasma was measured with colorimetry, turbidimetry and nitric acid reductase, respectively. The number of circulating endothelial progenitor cells(CEPCs) in peripheral blood was analyzed by CD133+/KDR+ antibodies and flow cytometry. Results Ninety cases underwent questionnaires. Between the groups, the difference of the levels of MDA (61.1, 65.5, 67.5 μmol/kg), O-·2 (4774.6, 5143.3, 4736.0 U/kg) ,CRP[(5.92 ± 2.44), (5.11 ± 2.40), (5.55 ± 2.96)mg/L], and NO[(659.8 ± 387.5), (667.4 ± 486.6), (762.1 ±763.2)μmol/kg], was not statistically significant (F =0.00, 0.46, 0.80, 0.47, all P ＞ 0.05). The difference of the number of CEPCs in different groups of arsenic in drinking water was statistically significant(0.96 x 10-5, 0.77 x 10-5,1.59 x 10-5, F=5.08, P＜ 0.05), where ＜ 10, 10 - 50 μg/L groups were significantly lower than ＞ 50 μg/L group (q =4.58, 6.65, all P ＜ 0.05). Conclusions The number of CEPCs in peripheral blood changes significantly with lower-arsenic exposure, whereas there are no obvious changes with the markers of oxidized damage and inflammation. This is the first human demonstration showing that lower-arsenic exposure may cause endothelial injury. Key words: Arsenic ; Endothelium, vascular; Circulating endothelial progenitor cells; Biomarkers

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低砷饮用水中早期血管内皮损伤的生物标志物

目的观察饮用水低砷暴露对内皮损伤早期有效生物标志物的影响。方法采用简单随机抽样的方法，在江苏省盱眙县沿河村居住10年以上的农村居民90名。将其家庭浅井砷含量分为< 10(32人)、10 - 50(28人)和> 50 μg/L(30人)3组。收集了个人的血液样本。测定了人血浆中丙二醛(MDA)与四丁基硫酸氢铵(TBA)的结合物。丙二醛被认为是监测脂质过氧化最重要的标志物。用比色法、浊度法和硝酸还原酶分别测定人血浆中抗超氧阴离子自由基(O-·2)、c反应蛋白(CRP)和NO水平。采用CD133+/KDR+抗体和流式细胞术分析外周血循环内皮祖细胞(CEPCs)的数量。结果90例接受问卷调查。两组间MDA(61.1、65.5、67.5 μmol/kg)、O-·2(4774.6、5143.3、4736.0 U/kg)、CRP[(5.92±2.44)、(5.11±2.40)、(5.55±2.96)mg/L]、NO[(659.8±387.5)、(667.4±486.6)、(762.1±763.2)μmol/kg]水平差异均无统计学意义(F =0.00、0.46、0.80、0.47,P均> 0.05)。不同砷组饮用水中CEPCs数量差异有统计学意义(0.96 × 10-5、0.77 × 10-5、1.59 × 10-5, F=5.08, P< 0.05)，其中< 10、10 ~ 50 μg/L组显著低于> 50 μg/L组(q =4.58、6.65，均P< 0.05)。结论低砷暴露后外周血CEPCs数量发生显著变化，而氧化损伤和炎症标志物变化不明显。这是人类首次证明低砷暴露可能导致内皮损伤。关键词:砷;血管内皮细胞;循环内皮祖细胞;生物标记物

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来源期刊

中华地方病学杂志我国对人类健康危害特别严重的地方性疾病：克山病、大骨节病、碘缺乏病、地方性氟中毒、地方性砷中毒、鼠疫、布鲁氏菌病、寄生虫、新冠肺炎等疾病，同时还报道多发性自然疫源性疾病。

CiteScore

1.60

自引率

0.00%

发文量

8714

期刊介绍： The Chinese Journal of Endemiology covers predominantly endemic diseases threatening health of the people in the areas affected by the diseases including Keshan disease, Kaschin-Beck Disease, iodine deficiency disorders, endemic fluorosis, endemic arsenism, plague, epidemic hemorrhagic fever, brucellosis, parasite diseases and the diseases related to local natural and socioeconomic conditions; and reports researches in the basic science, etiology, epidemiology, clinical practice, control as well as multidisciplinary studies on the diseases.