Role of apoptosis in hepatic injury of rat and patients with Clonorchis sinensis infection

Q3 Medicine

中华地方病学杂志 Pub Date : 2011-07-20 DOI:10.3760/CMA.J.ISSN.1000-4955.2011.04.005

Xiao-li Zhang, Yun-xia Dong, Su Han, Rong Da, Yihong Li, J. Shu, Fengmin Zhang

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引用次数: 0

Abstract

Objective To investigate the liver injury and pathological changes of rat and patients with Clonorchis sinensis(C, sinensis) infection, and to clarify the role of apoptosis in the injury induced by C. sinensis.Methods Wistar rats were divided into two group: 60 in infection group and 20 in control. The rats in infection group were infected with C. sinensis via oral feeding encysted cercaria;rats in control group were fed with normal saline. The rats were sacrificed 4, 6, 8 and 12 weeks after infection, respectively. Liver tissue specimens of the patients infected with C. sinensis were collected. The pathological changes of liver tissue were observed by light microscopy and the apoptofic rate of hepatocyte was detected by terminal deoxynucleotidyl transferase dUTP nick end labeling(TUNEL) assay. Results Parasites and eggs could he seen around the bile duct, and the duct was associated with mucosa and adenoma papillary hyperplasia, wall thickening, inflammatory cell infiltration, a small amount of fibrous tissue hyperplasia, and periportal liver cells surrounded by a number of nuclear condensation, all these changes meant morphological characteristics of apoptosis. Apoptotic rates of liver cells in infection group 4, 6,8 and 12 weeks after infection were (7.15 ± 1.50)%,(11.61 ± 3.09)%,(13.21 ± 3.47)% and (11.26 ± 4.06)%,respectively, which was significantly higher than that in control group [(2.57 ± 0.72)%, (3.17 + 0.77)%, (3.67 ±0.96)% and (2.84 ± 0.87)%, t values were 4.45, 5.49, 5.95 and 4.74, respectively, all P < 0.01]. Conclusions These findings indicate that C, sinensis can stimulate both hepatoeytic apoptosis and degeneration which may he related to clinical manifestations and liver lesions in patients with clonorchiasis. Key words: Clonorchis sinensis; Infection; Apoptosis; Hepatoeyte

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细胞凋亡在华支睾吸虫感染大鼠肝损伤中的作用

目的观察中华支睾吸虫(C, sinensis)感染大鼠及患者的肝脏损伤及病理变化，阐明细胞凋亡在中华支睾吸虫(C, sinensis)损伤中的作用。方法Wistar大鼠随机分为感染组60只，对照组20只。感染组大鼠采用囊状尾蚴灌胃感染中华梭菌，对照组大鼠灌胃生理盐水。分别于感染后4、6、8、12周处死。采集中华梭菌感染患者的肝组织标本。光镜下观察大鼠肝组织病理变化，末端脱氧核苷酸转移酶dUTP缺口末端标记法(TUNEL)检测肝细胞凋亡率。结果胆管周围可见寄生虫、虫卵，胆管伴粘膜及腺瘤乳头状增生，壁增厚，炎性细胞浸润，少量纤维组织增生，门静脉周围肝细胞周围有大量核凝聚，提示细胞凋亡的形态学特征。感染后4、6、8、12周感染组肝细胞凋亡率分别为(7.15±1.50)%、(11.61±3.09)%、(13.21±3.47)%、(11.26±4.06)%，均显著高于对照组[(2.57±0.72)%、(3.17 + 0.77)%、(3.67±0.96)%、(2.84±0.87)%，t值分别为4.45、5.49、5.95、4.74,P均< 0.01]。结论华支睾吸虫病患者的肝细胞凋亡和变性可能与临床表现和肝脏病变有关。关键词:华支睾吸虫;感染;细胞凋亡;Hepatoeyte

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来源期刊

中华地方病学杂志我国对人类健康危害特别严重的地方性疾病：克山病、大骨节病、碘缺乏病、地方性氟中毒、地方性砷中毒、鼠疫、布鲁氏菌病、寄生虫、新冠肺炎等疾病，同时还报道多发性自然疫源性疾病。

CiteScore

1.60

自引率

0.00%

发文量

8714

期刊介绍： The Chinese Journal of Endemiology covers predominantly endemic diseases threatening health of the people in the areas affected by the diseases including Keshan disease, Kaschin-Beck Disease, iodine deficiency disorders, endemic fluorosis, endemic arsenism, plague, epidemic hemorrhagic fever, brucellosis, parasite diseases and the diseases related to local natural and socioeconomic conditions; and reports researches in the basic science, etiology, epidemiology, clinical practice, control as well as multidisciplinary studies on the diseases.