Atla-Alternatives To Laboratory Animals最新文献_第4页

An Evaluation of the Replacement of Animal-derived Biomaterials in Human Primary Cell Culture. 评估人类原代细胞培养中动物源生物材料的替代性。

IF 2.4 4区医学

Atla-Alternatives To Laboratory Animals Pub Date : 2024-09-01 Epub Date: 2024-08-09 DOI: 10.1177/02611929241269004

Laura R Bramwell, Samantha J Gould, Merlin Davies, Conor McMullan, Emily C Trusler, Lorna W Harries

{"title":"An Evaluation of the Replacement of Animal-derived Biomaterials in Human Primary Cell Culture.","authors":"Laura R Bramwell, Samantha J Gould, Merlin Davies, Conor McMullan, Emily C Trusler, Lorna W Harries","doi":"10.1177/02611929241269004","DOIUrl":"10.1177/02611929241269004","url":null,"abstract":"The likelihood that potential new drugs will successfully navigate the current translational pipeline is poor, with fewer than 10% of drug candidates making this transition successfully, even after their entry into clinical trials. Prior to this stage, candidate drugs are typically evaluated by using models of increasing complexity, beginning with basic in vitro cell culture studies and progressing through to animal studies, where many of these candidates are lost due to lack of efficacy or toxicology concerns. There are many reasons for this poor translation, but interspecies differences in functional and physiological parameters undoubtedly contribute to the problem. Improving the human-relevance of early preclinical in vitro models may help translatability, especially when targeting more nuanced species-specific cell processes. The aim of the current study was to define a set of guidelines for the effective transition of human primary cells of multiple lineages to more physiologically relevant, translatable, animal-free in vitro culture conditions. Animal-derived biomaterials (ADBs) were systematically replaced with non-animal-derived alternatives in the in vitro cell culture systems, and the impact of the substitutions subsequently assessed by comparing the kinetics and phenotypes of the cultured cells. ADBs were successfully eliminated from primary human dermal fibroblast, uterine fibroblast, pulmonary fibroblast, retinal endothelial cell and peripheral blood mononuclear cell culture systems, and the individual requirements of each cell subtype were defined to ensure the successful transition toward growth under animal-free culture conditions. We demonstrate that it is possible to transition ('humanise') a diverse set of human primary cell types by following a set of simple overarching principles that inform the selection, and guide the evaluation of new, improved, human-relevant in vitro culture conditions.","PeriodicalId":55577,"journal":{"name":"Atla-Alternatives To Laboratory Animals","volume":" ","pages":"247-260"},"PeriodicalIF":2.4,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141910166","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Conference Diary. 会议日记。

IF 2.4 4区医学

Atla-Alternatives To Laboratory Animals Pub Date : 2024-09-01 DOI: 10.1177/02611929241274428

引用次数: 0

Development of Sensitive In Vitro Protocols for the Biocompatibility Testing of Medical Devices and Pharmaceuticals Intended for Contact with the Eyes: Acute Irritation and Phototoxicity Assessment. 开发用于与眼睛接触的医疗设备和药品生物兼容性测试的敏感体外协议：急性刺激和光毒性评估。

IF 2.4 4区医学

Atla-Alternatives To Laboratory Animals Pub Date : 2024-09-01 Epub Date: 2024-08-21 DOI: 10.1177/02611929241270095

Peter Pôbiš, Júlia Kubalcová, Tatiana Milasová, Helena Kandárová

{"title":"Development of Sensitive In Vitro Protocols for the Biocompatibility Testing of Medical Devices and Pharmaceuticals Intended for Contact with the Eyes: Acute Irritation and Phototoxicity Assessment.","authors":"Peter Pôbiš, Júlia Kubalcová, Tatiana Milasová, Helena Kandárová","doi":"10.1177/02611929241270095","DOIUrl":"10.1177/02611929241270095","url":null,"abstract":"This study introduces a novel in vitro methodology that employs the 3-D reconstructed tissue model, EpiOcular, to assess the irritation and phototoxicity potential of medical devices and drugs in contact with the eye. Our study evaluated diverse test materials, including medical devices, ophthalmological solutions and an experimental drug (cemtirestat), for their potential to cause eye irritation and phototoxicity. The protocols used in this study with the EpiOcular tissue model were akin to those used in the ultra-mildness testing of cosmetic formulations, which is challenging to predict with standard in vivo rabbit tests. To design these protocols, we leveraged experience gained from the validation project on the EpiDerm skin irritation test for medical devices (ISO 10993-23:2021) and the OECD TG 498 method for photo-irritation testing. The predictions were based on the tissue viability and inflammatory response, as determined by IL-1α release. By developing and evaluating these protocols for medical devices, we aimed to expand the applicability domain of the tests referred to in ISO 10993-23. This will contribute to the standardisation and cost-effective safety evaluation of ophthalmic products, while reducing reliance on animal testing in this field. The findings obtained from the EpiOcular model in the photo-irritation test could support its implementation in the testing strategies outlined in OECD TG 498.","PeriodicalId":55577,"journal":{"name":"Atla-Alternatives To Laboratory Animals","volume":" ","pages":"261-275"},"PeriodicalIF":2.4,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142019743","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Editorial. 社论

IF 2.4 4区医学

Atla-Alternatives To Laboratory Animals Pub Date : 2024-09-01 Epub Date: 2024-08-07 DOI: 10.1177/02611929241274885

Judith C Madden

引用次数: 0

Progress and Remaining Opportunities to Increase the Use of Animal-free Antibodies in the USA. 美国在增加使用无动物抗体方面取得的进展和仍然存在的机遇。

IF 2.4 4区医学

Atla-Alternatives To Laboratory Animals Pub Date : 2024-09-01 Epub Date: 2024-07-24 DOI: 10.1177/02611929241266472

Katherine Groff, David Allen, Warren Casey, Amy J Clippinger

{"title":"Progress and Remaining Opportunities to Increase the Use of Animal-free Antibodies in the USA.","authors":"Katherine Groff, David Allen, Warren Casey, Amy J Clippinger","doi":"10.1177/02611929241266472","DOIUrl":"10.1177/02611929241266472","url":null,"abstract":"The scientific and ethical issues associated with the use of animal-derived antibodies in research can be overcome by the use of animal-free, sequence-defined recombinant antibodies, whose benefits are well documented. Here, we describe progress made following a 2019 expert meeting focused on improving the quality and reproducibility of biomedical research by accelerating the production and use of animal-free recombinant antibodies in the USA. In the five intervening years since the meeting, participants have established multifaceted initiatives to tackle the next steps outlined during the meeting. These initiatives include: prioritising the replacement of ascites-derived and polyclonal antibodies; distributing educational materials describing recombinant antibodies; fostering public-private partnerships to increase access to recombinant antibodies; and increasing the availability of funding for recombinant antibody development. Given the widescale use of antibodies across scientific disciplines, a transition to modern antibody production methods relies on a commitment from government agencies, universities, industry and funding organisations, to initiatives such as those outlined here.","PeriodicalId":55577,"journal":{"name":"Atla-Alternatives To Laboratory Animals","volume":" ","pages":"285-289"},"PeriodicalIF":2.4,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141753467","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Conference Diary. 会议日记。

IF 2.4 4区医学

Atla-Alternatives To Laboratory Animals Pub Date : 2024-07-01 Epub Date: 2024-06-12 DOI: 10.1177/02611929241260577

引用次数: 0

2023 Michael Balls Award. 2023 年迈克尔-鲍尔斯奖

IF 2.4 4区医学

Atla-Alternatives To Laboratory Animals Pub Date : 2024-07-01 DOI: 10.1177/02611929241262162

引用次数: 0

An Accessible Nephrectomy and Cystotomy Simulator for Use in Teaching Small Animal Surgery. 用于小动物外科教学的无障碍肾切除术和膀胱切除术模拟器。

IF 2.4 4区医学

Atla-Alternatives To Laboratory Animals Pub Date : 2024-07-01 DOI: 10.1177/02611929241265103

Matheus Barbosa Gomes Cruz, Lyana Costa, Roberta Carareto, Simone Tostes de Oliveira Stedile

{"title":"An Accessible Nephrectomy and Cystotomy Simulator for Use in Teaching Small Animal Surgery.","authors":"Matheus Barbosa Gomes Cruz, Lyana Costa, Roberta Carareto, Simone Tostes de Oliveira Stedile","doi":"10.1177/02611929241265103","DOIUrl":"10.1177/02611929241265103","url":null,"abstract":"The use of simulators in veterinary education has been increasing over the last few years. This is fundamental for surgical classes, as simulators can provide a better learning environment for the students. Two procedures commonly taught in veterinary surgical practical classes are nephrectomy and cystotomy. However, the lack of simulators for use in these classes limits the training options to the use of cadavers, which have a number of associated disadvantages, including the potential for autolysis. The aim of this study was to develop and assess the value of a simple nephrectomy and cystotomy simulator that could replace the use of cadavers in surgical practical classes. The simulator, which represented the abdominal cavity, bladder, kidneys, ureter, vessels and adipose tissue, was constructed by using synthetic materials. To evaluate its usefulness and acceptance by the students, the learning outcomes and student satisfaction, for both the simulator and an ethically sourced dog cadaver, were compared. The students completed a Likert scale questionnaire, and the answers were evaluated by using the Diagnostic Content Validation (DCV) model. The simulator was well accepted by the students, with the best scores achieved for the ureter divulsion and ligation procedures; good results were also recorded for the kidney vessel ligature and urinary bladder suture practice. The scores showed that the simulator provided an acceptable experience during the training process and increased the confidence of the students in performing the procedure.","PeriodicalId":55577,"journal":{"name":"Atla-Alternatives To Laboratory Animals","volume":"52 4","pages":"214-223"},"PeriodicalIF":2.4,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141903652","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Spotlight on Three Rs Progress. 聚焦三个 R 的进展。

IF 2.4 4区医学

Atla-Alternatives To Laboratory Animals Pub Date : 2024-07-01 Epub Date: 2024-06-13 DOI: 10.1177/02611929241260578

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Resources Round-up. 资源综述。

IF 2.4 4区医学

Atla-Alternatives To Laboratory Animals Pub Date : 2024-07-01 Epub Date: 2024-06-12 DOI: 10.1177/02611929241260579

引用次数: 0