Atla-Alternatives To Laboratory Animals最新文献_第10页

Editorial. 社论

IF 2.7 4区医学

Atla-Alternatives To Laboratory Animals Pub Date : 2024-01-01 Epub Date: 2023-12-08 DOI: 10.1177/02611929231220133

Judith C Madden

引用次数: 0

Spotlight on Three Rs Progress. 聚焦三个 R 的进展。

IF 2.7 4区医学

Atla-Alternatives To Laboratory Animals Pub Date : 2024-01-01 Epub Date: 2023-12-05 DOI: 10.1177/02611929231218600

引用次数: 0

The 3Ranker: An AI-based Algorithm for Finding Non-animal Alternative Methods. The 3Ranker:一种基于人工智能的算法，用于寻找非动物的替代方法。

IF 2.7 4区医学

Atla-Alternatives To Laboratory Animals Pub Date : 2023-11-01 Epub Date: 2023-10-21 DOI: 10.1177/02611929231210777

Niels van Beuningen, Sinne Alkema, Nils Hijlkema, Brun Ulfhake, Rafael Frias, Merel Ritskes-Hoitinga, Wynand Alkema

{"title":"The 3Ranker: An AI-based Algorithm for Finding Non-animal Alternative Methods.","authors":"Niels van Beuningen, Sinne Alkema, Nils Hijlkema, Brun Ulfhake, Rafael Frias, Merel Ritskes-Hoitinga, Wynand Alkema","doi":"10.1177/02611929231210777","DOIUrl":"10.1177/02611929231210777","url":null,"abstract":"The search for existing non-animal alternative methods for use in experiments is currently challenging because of the lack of both comprehensive structured databases and balanced keyword-based search strategies to mine unstructured textual databases. In this paper we describe 3Ranker, which is a fast, keyword-independent algorithm for finding non-animal alternative methods for use in biomedical research. The 3Ranker algorithm was created by using a machine learning approach, consisting of a Random Forest model built on a dataset of 35 million abstracts and constructed with weak supervision, followed by iterative model improvement with expert curated data. We found a satisfactory trade-off between sensitivity and specificity, with Area Under the Curve (AUC) values ranging from 0.85-0.95. Trials showed that the AI-based classifier was able to identify articles that describe potential alternatives to animal use, among the thousands of articles returned by generic PubMed queries on dermatitis and Parkinson's disease. Application of the classification models on time series data showed the earlier implementation and acceptance of Three Rs principles in the area of cosmetics and skin research, as compared to the area of neurodegenerative disease research. The 3Ranker algorithm is freely available at www.open3r.org; the future goal is to expand this framework to cover multiple research domains and to enable its broad use by researchers, policymakers, funders and ethical review boards, in order to promote the replacement of animal use in research wherever possible.","PeriodicalId":55577,"journal":{"name":"Atla-Alternatives To Laboratory Animals","volume":" ","pages":"376-386"},"PeriodicalIF":2.7,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49685433","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Spotlight on Three Rs Progress. 聚焦Three Rs Progress。

IF 2.7 4区医学

Atla-Alternatives To Laboratory Animals Pub Date : 2023-11-01 Epub Date: 2023-10-15 DOI: 10.1177/02611929231206974

引用次数: 0

Optimisation of a Method for the Differentiation of Human Umbilical Cord-derived Mesenchymal Stem Cells Toward Renal Epithelial-like Cells. 人脐带来源的间充质干细胞向肾上皮样细胞分化的方法的优化。

IF 2.7 4区医学

Atla-Alternatives To Laboratory Animals Pub Date : 2023-11-01 Epub Date: 2023-10-13 DOI: 10.1177/02611929231207774

Rakhshinda Habib, Shumaila Fahim, Mohsin Wahid, Jahanara Ainuddin

{"title":"Optimisation of a Method for the Differentiation of Human Umbilical Cord-derived Mesenchymal Stem Cells Toward Renal Epithelial-like Cells.","authors":"Rakhshinda Habib, Shumaila Fahim, Mohsin Wahid, Jahanara Ainuddin","doi":"10.1177/02611929231207774","DOIUrl":"10.1177/02611929231207774","url":null,"abstract":"Human umbilical cord-derived mesenchymal stem cells (hucMSCs) can differentiate into multiple cell lineages, but few methods have been developed to generate kidney lineage cells. Due to their human origin, pluripotent nature and immunomodulatory properties, these stem cells are attractive candidates for clinical applications such as the repair or regeneration of damaged organs. This study evaluated the renal differentiation potential of hucMSCs, when exposed for 10 days to optimised concentrations of retinoic acid, activin-A and bone morphogenetic protein-7 (BMP-7) in various combinations, with and without the priming of the cells with a Wnt signalling pathway activator (CHIR99021). The hucMSCs were isolated and characterised according to surface marker expression (CD73, CD90, CD44, CD146 and CD8) and tri-lineage differentiation potential. The expression of key marker genes (OSR1, TBXT, HOXA13, SIX2, PAX2, KRT18 and ZO1) was examined by qRT-PCR. Specific marker protein expression (E-cadherin, cytokeratin-8 and cytokeratin-19) was analysed by immunocytochemistry. CHIR99021-primed cells treated with the retinoic acid, activin-A and BMP-7 cocktail showed epithelial cell-like differentiation - i.e. distinct phenotypic changes, as well as upregulated gene and protein expression, were observed that were consistent with an epithelial cell phenotype. Thus, our results showed that hucMSCs can efficiently differentiate into renal epithelial-like cells. This work may help in the development of focused therapeutic strategies, in which lineage-defined human stem cells can be used for renal regeneration.","PeriodicalId":55577,"journal":{"name":"Atla-Alternatives To Laboratory Animals","volume":" ","pages":"363-375"},"PeriodicalIF":2.7,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41221023","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Barriers to the Use of Recombinant Bacterial Endotoxins Test Methods in Parenteral Drug, Vaccine and Device Safety Testing. 肠外药物、疫苗和器械安全性测试中使用重组细菌内毒素测试方法的障碍。

IF 2.7 4区医学

Atla-Alternatives To Laboratory Animals Pub Date : 2023-11-01 Epub Date: 2023-10-19 DOI: 10.1177/02611929231204782

Elizabeth Baker, Jessica Ponder, Johannes Oberdorfer, Ingo Spreitzer, Jay Bolden, Marine Marius, Thierry Bonnevay, Kristie Sullivan

{"title":"Barriers to the Use of Recombinant Bacterial Endotoxins Test Methods in Parenteral Drug, Vaccine and Device Safety Testing.","authors":"Elizabeth Baker, Jessica Ponder, Johannes Oberdorfer, Ingo Spreitzer, Jay Bolden, Marine Marius, Thierry Bonnevay, Kristie Sullivan","doi":"10.1177/02611929231204782","DOIUrl":"10.1177/02611929231204782","url":null,"abstract":"The Bacterial Endotoxins Test (BET) is a critical safety test that is used to detect bacterial endotoxins, which are the major contributor to fever-inducing contamination risks known as pyrogens. All parenteral therapies, including every lot of injected drugs, vaccines, medical devices, must be tested for pyrogens to ensure patient safety. Bacterial endotoxins test methods were developed as a highly sensitive detection method for bacterial endotoxins, after the discovery of a clotting cascade in horseshoe crab blood. However, horseshoe crab species are limited to some inshore coastal habitats along the Atlantic coast of the USA and others throughout Asia. Fully functional horseshoe crab clotting factors can be manufactured via recombinant protein production, and several BET methods featuring recombinant horseshoe crab proteins have now been developed for commercial use. Recombinant Bacterial Endotoxins Test (rBET) methods based on the use of recombinant Factor C (rFC) were established in the European Pharmacopoeia - however, these methods have not yet been granted compendial status in the United States Pharmacopoeia (USP). In order to facilitate dialogue between stakeholders, the Physicians Committee for Responsible Medicine hosted two virtual roundtable discussions on the perceived barriers to the use of rBET methods for US FDA requirements. Stakeholders agreed that multiple rFC-based methods have been demonstrated to have suitable analytical performance, as described in ICH Q2 on the Validation of Analytical Procedures and USP <1225> on the Validation of Compendial Procedures. United States Pharmacopoeia compendial inclusion of the rFC-based and other rBET methods was favoured, in order to reduce the additional burdens created by a lack of global harmonisation on BET testing requirements.","PeriodicalId":55577,"journal":{"name":"Atla-Alternatives To Laboratory Animals","volume":" ","pages":"401-410"},"PeriodicalIF":2.7,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49685432","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Development of an In Vitro Sensitisation Test Using a Coculture System of Human Bronchial Epithelium and Immune Cells. 使用人支气管上皮和免疫细胞的共培养系统开发体外致敏试验。

IF 2.7 4区医学

Atla-Alternatives To Laboratory Animals Pub Date : 2023-11-01 Epub Date: 2023-10-05 DOI: 10.1177/02611929231204823

Ikuya Tanabe, Kunitaka Yoshida, Shinkichi Ishikawa, Kanae Ishimori, Tsuneo Hashizume, Takayuki Yoshimoto, Takao Ashikaga

{"title":"Development of an In Vitro Sensitisation Test Using a Coculture System of Human Bronchial Epithelium and Immune Cells.","authors":"Ikuya Tanabe, Kunitaka Yoshida, Shinkichi Ishikawa, Kanae Ishimori, Tsuneo Hashizume, Takayuki Yoshimoto, Takao Ashikaga","doi":"10.1177/02611929231204823","DOIUrl":"10.1177/02611929231204823","url":null,"abstract":"Chemical respiratory sensitisation is a serious health problem. However, to date, there are no validated test methods available for identifying respiratory sensitisers. The aim of this study was to develop an in vitro sensitisation test by modifying the human cell line activation test (h-CLAT) to detect respiratory sensitisers and distinguish them from skin sensitisers. THP-1 cells were exposed to the test chemicals (two skin sensitisers and six respiratory sensitisers), either as monocultures or as cocultures with air-liquid interface-cultured reconstructed human bronchial epithelium. The responses were analysed by measuring the expression levels of surface markers on THP-1 cells (CD86, CD54 and OX40L) and the concentrations of cytokines in the culture media (interleukin (IL)-8, IL-33 and thymic stromal lymphopoietin (TSLP)). The cocultures exhibited increased CD54 expression on THP-1 cells; moreover, in the cocultures but not in the monocultures, exposure to two uronium salts (i.e. respiratory sensitisers) increased CD54 expression on THP-1 cells to levels above the criteria for a positive h-CLAT result. Additionally, exposure to the respiratory sensitiser abietic acid, significantly increased IL-8 concentration in the culture medium, but only in the cocultures. Although further optimisation of the method is needed to distinguish respiratory from skin sensitisers by using these potential markers (OX40L, IL-33 and TSLP), the coculture of THP-1 cells with bronchial epithelial cells offers a potentially useful approach for the detection of respiratory sensitisers.","PeriodicalId":55577,"journal":{"name":"Atla-Alternatives To Laboratory Animals","volume":" ","pages":"387-400"},"PeriodicalIF":2.7,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41158362","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Conference Diary. 会议日记。

IF 2.7 4区医学

Atla-Alternatives To Laboratory Animals Pub Date : 2023-11-01 Epub Date: 2023-10-25 DOI: 10.1177/02611929231206952

引用次数: 0

Resources Round-up. 资源汇总。

IF 2.7 4区医学

Atla-Alternatives To Laboratory Animals Pub Date : 2023-11-01 Epub Date: 2023-10-16 DOI: 10.1177/02611929231206955

引用次数: 0

Editorial. 社论

IF 2.7 4区医学

Atla-Alternatives To Laboratory Animals Pub Date : 2023-11-01 Epub Date: 2023-10-15 DOI: 10.1177/02611929231206692

Judith C Madden

引用次数: 0