4区生物学

Biocell Pub Date : 2024-01-01 DOI: 10.32604/biocell.2024.054364

Pinghua Tu, Shanshan Wang, KELAN DENG, Xinjun Li, ZHANLING WU

引用次数: 0

SOX1 promotes osteosarcoma metastasis by modulating TSPAN12 expression SOX1通过调节TSPAN12表达促进骨肉瘤转移

4区生物学

Biocell Pub Date : 2024-01-01 DOI: 10.32604/biocell.2024.052670

HEYI LIU, WENHAO CHENG, Jingliang He, Luyao Zhang, Kadirya Asan, Y.- L. Chen, JIAYUN WANG, Qi Gao, SENG WANG, ZIEN YU, Shaojie Ma, Zhu Lan, Jing Ji

引用次数: 0

Oleanolic acid inhibits colon cancer cell stemness and reverses chemoresistance by suppressing JAK2/STAT3 signaling pathway 齐墩果酸通过抑制JAK2/STAT3信号通路抑制结肠癌细胞的干细胞性并逆转化疗耐药

4区生物学

Biocell Pub Date : 2024-01-01 DOI: 10.32604/biocell.2024.051074

RUOYU CHEN, YIMAN WU, FENG WANG, JUNTAO ZHOU, HUAZHANG ZHUANG, Li Wei

{"title":"Oleanolic acid inhibits colon cancer cell stemness and reverses chemoresistance by suppressing JAK2/STAT3 signaling pathway","authors":"RUOYU CHEN, YIMAN WU, FENG WANG, JUNTAO ZHOU, HUAZHANG ZHUANG, Li Wei","doi":"10.32604/biocell.2024.051074","DOIUrl":"https://doi.org/10.32604/biocell.2024.051074","url":null,"abstract":"Background: Oleanolic acid (OA), a pentacyclic triterpenoid exhibiting specific anti-cancer properties and highly effective antioxidant activity, was isolated from traditional Chinese medicinal herbs. Conversely, the OA that impacts colon cancer (CC) cells and its underlying mechanisms remain poorly understood. Methods: The cytotoxic effect of OA alone or OA-5-Fluorouracil (5-FU) combination on normal and CC cells was analyzed by methyl thiazolyl diphenyl-tetrazolium bromide (MTT). Then, the impact of OA on CC cell lines (LoVo and HT-29) proliferation and stemness were measured using colon formation and tumorsphere formation assays. Octamer-binding transcription factor 4 (Oct4), Prominin-1 (CD133), Nanog, and transcription factor SOX-2 (SOX2) are cell stemness-related indicators whose expression was assessed using fluorescence qPCR assay, Western blotting, and immunohistochemistry. The effect of OA on the proliferative potency of CC cells was evaluated using an in vivo model. Results: The stem-like characteristics and clone production of colon cancer cells were markedly reduced by OA alone or in combination with OA-5-FU. Moreover, OA increases the susceptibility of CC cells to 5-FU by blocking the cell stemness-related markers (CD133, Nanog, SOX2, and Oct4) expression levels both in vitro and in vivo, as well as by inactivating the activator of transcription 3 (STAT3 signaling) and Janus kinase 2/signal transducer (JAK2). Conclusion: These findings imply that oleanolic acid, both in vitro and in vivo, suppresses the JAK2/STAT3 pathway, which in turn reverses chemoresistance and decreases colon cancer cell stemness. Therefore, by reducing the recommended amount of 5-FU, this strategy may improve chemotherapeutic effectiveness and minimize undesired side effects.","PeriodicalId":55384,"journal":{"name":"Biocell","volume":"48 7","pages":"1037-1046"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.techscience.com/biocell/online/detail/20622/pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147895580","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

OPA3 overexpression modulates lipid droplet production and sensitizes colorectal cancer cells to bevacizumab treatment OPA3过表达调节脂滴的产生并使结直肠癌细胞对贝伐单抗治疗敏感

4区生物学

Biocell Pub Date : 2024-01-01 DOI: 10.32604/biocell.2024.049466

Hongbiao Wu, DONGFANG LIU

引用次数: 0

LA-D-B1, a novel Abemaciclib derivative, exerts anti-breast cancer effects through CDK4/6 LA-D-B1是一种新的Abemaciclib衍生物，通过CDK4/6发挥抗乳腺癌作用

4区生物学

Biocell Pub Date : 2024-01-01 DOI: 10.32604/biocell.2024.050868

LING MA, ZIRUI JIANG, Xiao Hou, YUTING XU, ZIYUN CHEN, SIYI ZHANG, HANXUE LI, Shaojie Ma, Geng Zhang, X. Wang, Jing Ji

引用次数: 3

The regulatory role of melatonin in pituitary thyroid-stimulating hormone synthesis through casein kinase 1α 褪黑素通过酪蛋白激酶1α对垂体促甲状腺激素合成的调节作用

4区生物学

Biocell Pub Date : 2024-01-01 DOI: 10.32604/biocell.2023.044630

BINGJIE WANG, YEWEN ZHOU, Tongjuan Niu, Mengqing Yin, Sheng Cui

引用次数: 1

Reversal of maternal obesity attenuates hypoxia and improves placental development in the preeclamptic-like BPH/5 mouse model. 在先兆子痫样BPH/5小鼠模型中，逆转母体肥胖可减轻缺氧并改善胎盘发育。

IF 1.2 4区生物学

Biocell Pub Date : 2023-09-28 DOI: 10.32604/biocell.2023.029644

Daniella M Adams, Kalie F Beckers, Juliet P Flanagan, Viviane C L Gomes, Chin-Chi Liu, Jenny L Sones

{"title":"Reversal of maternal obesity attenuates hypoxia and improves placental development in the preeclamptic-like BPH/5 mouse model.","authors":"Daniella M Adams, Kalie F Beckers, Juliet P Flanagan, Viviane C L Gomes, Chin-Chi Liu, Jenny L Sones","doi":"10.32604/biocell.2023.029644","DOIUrl":"10.32604/biocell.2023.029644","url":null,"abstract":"Background: Women with obesity have higher risk of adverse pregnancy outcomes, including preeclampsia (PE). Late-gestational hypertension, aberrant fetoplacental development, and fetal growth restriction (FGR), hallmarks of PE, are observed spontaneously in BPH/5 mice. Similar to obese preeclamptic women, BPH/5 mice have higher visceral white adipose tissue (WAT) and circulating leptin. We hypothesized that attenuation of maternal obesity and serum leptin in pregnant BPH/5 mice will improve fetoplacental development by decreasing hypoxia markers and leptin expression at the maternal-fetal interface.Methods: To test this hypothesis, BPH/5 mice were fed ad libitum (lib) and pair-fed (PF) to C57 ad lib controls beginning at embryonic day (e) 0.5. Hypoxia-related genes, hypoxia inducible factor (Hif) 1α, stem cell factor (Scf), heme oxygenase-1 (Ho-1), leptin (Lep), and leptin receptor (LepR) were assessed in e7.5 implantation sites.Results: BPH/5 ad lib had 1.5 to 2-fold increase in Hif1α, Scf, and Ho-1 mRNA and a greater than 3-fold increase in leptin mRNA vs. C57 that was attenuated with PF. Exogenous leptin promoted Hif1α and Ho-1 mRNA expression in e7.5 decidua in vitro. While hypoxic conditions in vitro did not change decidual leptin mRNA. Furthermore, BPH/5 PF mice demonstrated improved fetal and placental outcomes later in gestation, with greater placental vascular area by e18.5 and attenuation of FGR.Conclusion: In conclusion, pair-feeding BPH/5 mice beginning at conception may improve placental vasculature formation via decreased leptin and hypoxia-associated markers in this model. Future investigations are needed to better determine the effect of hypoxia and leptin on pregnancy outcomes in obese pregnant women.","PeriodicalId":55384,"journal":{"name":"Biocell","volume":"47 9","pages":"2051-2058"},"PeriodicalIF":1.2,"publicationDate":"2023-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10569287/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41220811","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Anti-proliferative effect of Annona extracts on breast cancer cells. 番红花提取物对乳腺癌症细胞的抗增殖作用。

IF 0.8 4区生物学

Biocell Pub Date : 2023-08-28

Maria-Luisa Veisaga, Mariam Ahumada, Stacy Soriano, Leonardo Acuna, Wei Zhang, Ivy Leung, Robert Barnum, Manuel A Barbieri

{"title":"Anti-proliferative effect of Annona extracts on breast cancer cells.","authors":"Maria-Luisa Veisaga, Mariam Ahumada, Stacy Soriano, Leonardo Acuna, Wei Zhang, Ivy Leung, Robert Barnum, Manuel A Barbieri","doi":"","DOIUrl":"","url":null,"abstract":"Backgorund: Fruits and seed extracts of Annona montana have significant cytotoxic potential in several cancer cells. This study evaluates the effect of A. montana leaves hexane extract on several signaling cascades and gene expression in metastatic breast cancer cells upon insulin-like growth factor-1 (IGF-1) stimulation.Methods: MTT assay was performed to determine the proliferation of cancer cells. Propidium iodide staining and flow cytometry analysis of Annexin V binding was utilized to measure the progression of the cell cycle and the induction of apoptosis. Protein expression and phosphorylation were determined by western blotting analysis to examine the underlying cellular mechanism triggered upon treatment with A. montana leaves hexane extract.Results: A. montana leaves hexane (sub-fraction V) blocked the constitutive stimulation of the PI3K/mTOR signaling pathways. This inhibitory effect was associated with apoptosis induction as evidenced by the positivity with Annexin V and terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNNEL) staining, activation of caspase-3, and cleavage of PPAR. It also limited the expression of various downstream genes that regulate proliferation, survival, metastasis, and angiogenesis (i.e., cyclin D1, survivin, COX-2, and VEGF). It increased the expression of p53 and p21. Interestingly, we also observed that this extract blocked the activation of AKT and ERK without affecting the phosphorylation of the IGF-1 receptor and activation of Ras upon IGF-1 stimulation.Conclusion: Our study indicates that A. montana leaves (sub-fraction V) extract exhibits a selective anti-proliferative and proapoptotic effect on the metastatic MDA-MB-231 breast cancer cells through the involvement of PI3K/AKT/mTOR/S6K1 pathways.","PeriodicalId":55384,"journal":{"name":"Biocell","volume":"47 8","pages":"1835-1852"},"PeriodicalIF":0.8,"publicationDate":"2023-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10538365/pdf/nihms-1927735.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41152106","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of non-enzymatic glycation on collagen nanoscale mechanisms in diabetic and age-related bone fragility. 非酶糖基化对糖尿病和年龄相关骨脆性中胶原纳米级机制的影响。

IF 1.2 4区生物学

Biocell Pub Date : 2023-06-21 DOI: 10.32604/biocell.2023.028014

James L Rosenberg, William Woolley, Ihsan Elnunu, Julia Kamml, David S Kammer, Claire Acevedo

{"title":"Effect of non-enzymatic glycation on collagen nanoscale mechanisms in diabetic and age-related bone fragility.","authors":"James L Rosenberg, William Woolley, Ihsan Elnunu, Julia Kamml, David S Kammer, Claire Acevedo","doi":"10.32604/biocell.2023.028014","DOIUrl":"https://doi.org/10.32604/biocell.2023.028014","url":null,"abstract":"Age and diabetes have long been known to induce an oxidative reaction between glucose and collagen, leading to the accumulation of advanced glycation end-products (AGEs) cross-links in collagenous tissues. More recently, AGEs content has been related to loss of bone quality, independent of bone mass, and increased fracture risk with aging and diabetes. Loss of bone quality is mostly attributed to changes in material properties, structural organization, or cellular remodeling. Though all these factors play a role in bone fragility disease, some common recurring patterns can be found between diabetic and age-related bone fragility. The main pattern we will discuss in this viewpoint is the increase of fibrillar collagen stiffness and loss of collagen-induced plasticity with AGE accumulation. This study focused on recent related experimental studies and discusses the correlation between fluorescent AGEs content at the molecular and fibrillar scales, collagen deformation mechanisms at the nanoscale, and resistance to bone fracture at the macroscale.","PeriodicalId":55384,"journal":{"name":"Biocell","volume":"47 7","pages":"1651-1659"},"PeriodicalIF":1.2,"publicationDate":"2023-06-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10486207/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10293963","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The antioxidant trolox inhibits aging and enhances prostaglandin E-2 secretion in mesenchymal stem cells 抗氧化剂trolox抑制衰老，促进间充质干细胞分泌前列腺素E-2

IF 1.2 4区生物学

Biocell Pub Date : 2023-01-01 DOI: 10.32604/biocell.2023.025203

Xiaoxu Zhang, Lin Zhang, Lin Du, Huiyan Sun, Xia Zhao, Yang Sun, Wen Wang, Lisheng Wang

引用次数: 0

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