Biology Bulletin最新文献_第4页

Reference Gene Selection for Expression Analysis of Hepatic Genes Responding to Fasting via Transcriptomic Analysis 通过转录组分析为空腹反应的肝脏基因表达分析选择参考基因

IF 0.5 4区生物学

Biology Bulletin Pub Date : 2024-07-13 DOI: 10.1134/s1062359024607110

Tianheng Zhao, Meng Tie, Mengyue Wu, Anyuan He, Yali Chen

{"title":"Reference Gene Selection for Expression Analysis of Hepatic Genes Responding to Fasting via Transcriptomic Analysis","authors":"Tianheng Zhao, Meng Tie, Mengyue Wu, Anyuan He, Yali Chen","doi":"10.1134/s1062359024607110","DOIUrl":"https://doi.org/10.1134/s1062359024607110","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3>The liver of mammals is a critical organ that houses numerous physiological processes, including macronutrient metabolism. Regarding nutrient metabolism, the liver shows a quick response to nutrient status fluctuation, such as feeding and fasting. Indeed, fasting is a powerful tool to uncover the molecular mechanism through which the expression of hepatic genes is regulated. The most widely applied approach to test gene expression at the RNA level is relative quantitative polymerase chain reaction (qPCR) using stably expressed genes as an internal reference. In this study, we leveraged transcriptomic analysis to screen the hepatic genes whose expression levels were stable in response to fasting. Our data suggested that expression levels of several well-known reference genes such as beta-actin and Eef1a1 are dramatically altered upon fasting. Further assessment indicated that Itm2b is the most stably expressed hepatic gene showing no alteration to fasting. Compared to beta-actin and Eef1a1, similar results and conclusions as transcriptomic analysis were obtained using Itm2b as an internal reference in qPCR when we assessed the effect of fasting on the expression of genes involved in peroxisomal fatty acid oxidation, fatty acid synthesis, and gluconeogenesis.","PeriodicalId":55366,"journal":{"name":"Biology Bulletin","volume":null,"pages":null},"PeriodicalIF":0.5,"publicationDate":"2024-07-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141609211","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biodiversity of Epibenthic Invertebrates in the Subtidal Mekong Estuaries, Southern Vietnam with Remarks on Decapod Assemblages 越南南部湄公河潮下河口底栖无脊椎动物的生物多样性及有关十足目动物群落的说明

IF 0.5 4区生物学

Biology Bulletin Pub Date : 2024-07-13 DOI: 10.1134/s1062359024606542

Thai T. Tran, Tho V. Le, Hieu H. Ha, Hoai. N. Pham, Hien T. Nguyen, To Uyen T. Nguyen, Quang X. Ngo, Luu T. Pham

{"title":"Biodiversity of Epibenthic Invertebrates in the Subtidal Mekong Estuaries, Southern Vietnam with Remarks on Decapod Assemblages","authors":"Thai T. Tran, Tho V. Le, Hieu H. Ha, Hoai. N. Pham, Hien T. Nguyen, To Uyen T. Nguyen, Quang X. Ngo, Luu T. Pham","doi":"10.1134/s1062359024606542","DOIUrl":"https://doi.org/10.1134/s1062359024606542","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3>Epibenthic invertebrates in three estuaries of the Mekong River, namely Co Chien, Ba Lai, and Cua Dai, were sampled for ecological analysis in November 2022. The objectives of this research were to provide the first baseline survey of epibenthic communities in the Mekong estuaries and to describe more precisely the relationships between epibenthic characteristics and environmental parameters. In total, seven major taxa, including Bivalvia, Decapoda, Gastropoda, Isopoda, Molpadida, Stomatopoda, and Xiphosura were recorded in this estuarine system. The abundances of the epibenthic invertebrates range from 48 to 176 inds/1000 m2. Decapos always occupy the highest numbers with a percentage ranging from 86‒95%. There are 24 decapod species were recorded in this study with the following as dominant families Palaemonidae, Penaeidae, and Paguridae. Our findings highlight that the diversity of epibenthic communities, particularly for decapods, is notably high; however, the epibenthic density is considerably low. Epibenthic density and diversity were significantly correlated with salinity, TDS, depth, and pH but also other factors may play a role. The assessment of epibenthic assemblage diversity and its correlation with environmental factors holds significant importance in the formulation of conservation strategies.","PeriodicalId":55366,"journal":{"name":"Biology Bulletin","volume":null,"pages":null},"PeriodicalIF":0.5,"publicationDate":"2024-07-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141609075","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of Bacillus thuringiensis var. israelensis Strains and Toxins on the Pine Processionary Moth Thaumetopoea wilkinsoni (Lepidoptera: Notodontidae) 苏云金芽孢杆菌变种 Israelensis 菌株和毒素对松树游蛾 Thaumetopoea wilkinsoni（鳞翅目：夜蛾科）的影响

IF 0.5 4区生物学

Biology Bulletin Pub Date : 2024-07-13 DOI: 10.1134/s1062359024607250

Elif-Fatma Topkara, Oğuzhan Yanar, Tuğrul Doruk, Yüksel Terzi

{"title":"Effects of Bacillus thuringiensis var. israelensis Strains and Toxins on the Pine Processionary Moth Thaumetopoea wilkinsoni (Lepidoptera: Notodontidae)","authors":"Elif-Fatma Topkara, Oğuzhan Yanar, Tuğrul Doruk, Yüksel Terzi","doi":"10.1134/s1062359024607250","DOIUrl":"https://doi.org/10.1134/s1062359024607250","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3>The pine processionary moth Thaumetopoea wilkinsoni (Tams, 1926) (Lepidoptera: Notodontidae) is one of the most harmful species that causes destruction in pine ecosystems and also causes critical skin reactions in humans and animals due to its urticating hairs. This study aimed to evaluate the efficacy of different strains [Bti ATCC 35646 (wild-type strain), Bti pHT315, and Bti pHTppk (mutant strain)] of Diptera-targeted Bacillus thuringiensis subsp. israelensis (Bti) and toxins (spore/crystal mixtures) obtained from these strains against the 4th larval stage of T. wilkinsoni under laboratory conditions. T. wilkinsoni eggs were collected from pine trees at Ondokuz Mayıs University in Samsun, Turkey, in 2022. Pine needles were contaminated with bacterial strains of different concentrations (1 × 107, 1 × 108, 1 × 109 cfu/mL) in 5 mL and toxins in different concentrations (0.15, 0.3, 0.6, 1.25, 2.5, 5 mg/mL) in 2 mL. Larvae were placed in containers with 30 larvae in each group and were observed for 25 days. As a result of the study, Bti ATCC 35646 with 63.4% mortality and the lowest LC50 value of 1.2 × 109 cfu/mL among the three strains of Bti and Bti pHTppk with 76.7% mortality and the lowest LC50 value of 1.2 mL among the toxins from the Bti strains had found to be virulent for T. wilkinsoni. The results obtained from our study suggest that Diptera-targeted Bti is virulent to T. wilkinsoni and that this strain and spore/crystal mixture can be used in the biological control of this species.","PeriodicalId":55366,"journal":{"name":"Biology Bulletin","volume":null,"pages":null},"PeriodicalIF":0.5,"publicationDate":"2024-07-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141609079","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

DNA Barcoding of Tree Frogs: Testing the Existence of Two Mitochondrial Lineages of Hyla savignyi 树蛙的 DNA 条形码：测试 Hyla savignyi 是否存在两个线粒体系谱

IF 0.5 4区生物学

Biology Bulletin Pub Date : 2024-07-13 DOI: 10.1134/s1062359024606980

T. Ergül Kalaycı

引用次数: 0

The Status and Distributions of the Wild Olive (Olea europaea subsp. europaea var. sylvestris) in Muğla Province and Its Islands under Global Climate Change 全球气候变化下穆拉省及其岛屿野生橄榄（Olea europaea subsp.

IF 0.5 4区生物学

Biology Bulletin Pub Date : 2024-07-13 DOI: 10.1134/s1062359023606419

Belgin Gocmen Taskin, Vatan Taskin, Kenan Akbaş

{"title":"The Status and Distributions of the Wild Olive (Olea europaea subsp. europaea var. sylvestris) in Muğla Province and Its Islands under Global Climate Change","authors":"Belgin Gocmen Taskin, Vatan Taskin, Kenan Akbaş","doi":"10.1134/s1062359023606419","DOIUrl":"https://doi.org/10.1134/s1062359023606419","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3>The olive is an economically important and indispensable species of the Mediterranean basin with remarkable historic and cultural significance. Wild olives constitute a priceless reservoir of genetic diversity which has great value for olive breeding and conservation programs. However, global climate changes especially global warming is expected to threaten olive cultivation in future causing large economic loses in the region. Therefore, knowledge about distribution and abundance of wild olive populations is important to maximize their conservation, safeguard, and exploitation. Muğla province, where the wild olives make up an important part of the vegetation, is one of the main olive producing area in Turkey with many towns and villages dependent on the olive. In this study, our aim is to examine the current geographical distribution and density of wild olive populations in this province and the 82 islands around its coast. The results demonstrated that young olives, which show good adaptation to region, just have started to expand to the higher parts of the Kavaklıdere district. In addition, in most of the islands wild olives are preserved in their undisturbed natural habitats where they exist as small groves or scattered plants. The results of this study provides important insights for the conservation of wild olive genetic resources. The data reported here, are based on actual field data thus the geographic coverage tends to be complete and consistent.","PeriodicalId":55366,"journal":{"name":"Biology Bulletin","volume":null,"pages":null},"PeriodicalIF":0.5,"publicationDate":"2024-07-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141609076","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Impact of the Anatolian Diagonal on Biodiversity in Turkey: A Geometric Morphometric Study on the Wing Morphology of Platycnemis dealbata (Insecta: Odonata) 安纳托利亚对角线对土耳其生物多样性的影响：蟪蛄（昆虫纲：鸟纲）翅膀形态的几何形态计量学研究

IF 0.5 4区生物学

Biology Bulletin Pub Date : 2024-07-13 DOI: 10.1134/s1062359023605827

A. Salur, A. Miroglu, G. K. Akyıldız

引用次数: 0

Age and Body Size of the Pamphylian Green Lizard, Lacerta pamphylica (Schmidtler, 1975) 帕姆菲利亚绿蜥蜴 Lacerta pamphylica 的年龄和体型（施密特勒，1975 年）

IF 0.5 4区生物学

Biology Bulletin Pub Date : 2024-07-13 DOI: 10.1134/s1062359024600272

Hatice Özkan, Ufuk Bülbül

引用次数: 0

Histopathological and Biochemical Alterations in the Adrenal Gland of Male Rats Exposed to Bisphenol F 暴露于双酚 F 的雄性大鼠肾上腺的组织病理学和生化变化

IF 0.5 4区生物学

Biology Bulletin Pub Date : 2024-05-31 DOI: 10.1134/s1062359023606717

B. Kaptaner, A. Doğan, C. Yılmaz, H. Aykut, E. Doğan, C. Fidan Babat, F. Dönmez

{"title":"Histopathological and Biochemical Alterations in the Adrenal Gland of Male Rats Exposed to Bisphenol F","authors":"B. Kaptaner, A. Doğan, C. Yılmaz, H. Aykut, E. Doğan, C. Fidan Babat, F. Dönmez","doi":"10.1134/s1062359023606717","DOIUrl":"https://doi.org/10.1134/s1062359023606717","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3>Bisphenol F (BPF) is a bisphenol A derivative that is widely used in the manufacturing of industrial and consumer products. The presented study was conducted to explore the adverse effects of BPF on the adrenal glands of rats. Toward this aim, twenty-eight male albino Wistar rats were randomly divided into four groups, each containing seven individuals. The animals were administered a vehicle (control) or BPF at 3 different doses comprising 20, 100, and 500 mg/kg of body weight (bw) via oral gavage for 28 days. Next, the effects of BPF on the gland were evaluated in terms of histopathological alterations, serum adrenocorticotropic hormone (ACTH) and cortisol levels, and the contents of reduced glutathione (GSH) and malondialdehyde (MDA). The zona fasciculata of the adrenal cortex showed prominent histopathological lesions such as vacuolar degeneration and necrotic degeneration in the experimental groups. BPF caused noticeable elevations in the serum ACTH and cortisol levels. Moreover, decreases in levels of GSH and increments in the contents of MDA were determined, indicating oxidative stress. In conclusion, BPF showed toxic effects on the adrenal gland that may result in disruptions in its vital functions in the body. The results herein suggest that attention should be paid to the utilization of BPF in manufacturing processes due to its harmful effects and risks to the health of organisms.","PeriodicalId":55366,"journal":{"name":"Biology Bulletin","volume":null,"pages":null},"PeriodicalIF":0.5,"publicationDate":"2024-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141255367","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biosynthesis and Characterization of Silver Nanoparticles Mediated by Cistus salviifolius L. and Ferula communis L. Extracts and Evaluation of Their Antioxidant, Antibacterial, and Cytotoxic Potentials 肉苁蓉（Cistus salviifolius L.）和阿魏（Ferula communis L.）提取物介导的银纳米粒子的生物合成和表征及其抗氧化、抗菌和细胞毒性潜力评估

IF 0.5 4区生物学

Biology Bulletin Pub Date : 2024-05-31 DOI: 10.1134/s1062359023603634

Funda Ulusu, Yakup Ulusu

{"title":"Biosynthesis and Characterization of Silver Nanoparticles Mediated by Cistus salviifolius L. and Ferula communis L. Extracts and Evaluation of Their Antioxidant, Antibacterial, and Cytotoxic Potentials","authors":"Funda Ulusu, Yakup Ulusu","doi":"10.1134/s1062359023603634","DOIUrl":"https://doi.org/10.1134/s1062359023603634","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3>In this research, we employed a microwave-assisted green synthesis method utilizing extracts from Cistus salviifolius L. and Ferula communis L. to synthesize silver nanoparticles (AgNPs). Subsequently, we characterized the biosynthesized AgNPs, both Cs-AgNPs (derived from C. salviifolius) and Fc-AgNPs (derived from F. communis), through UV-Vis, FT-IR, XRD, SEM, and TEM analyses. The AgNPs exhibited a spherical shape with sizes averaging below 11 nm. To assess their antioxidant activity, we employed the DPPH method. Furthermore, we examined the antimicrobial potential of Cs-AgNPs and Fc-AgNPs against pathogenic bacterial strains, including Bacillus subtilis, Staphylococcus aureus, Enterococcus faecalis, and Escherichia coli, using the disc diffusion assay and minimum inhibitory concentration. Additionally, we evaluated the cytotoxic effects of Cs-AgNPs and Fc-AgNPs on various cell lines, namely breast adenocarcinoma (MCF-7), colon carcinoma (HT-29), human keratinocytes (HaCaT), and embryonic kidney (HEK-293), employing the Alamar Blue assay. Our findings indicate that the biosynthesized Cs-AgNPs and Fc-AgNPs possess antioxidant, antibacterial, and anticancer activities. Therefore, the current study suggests that Cs-AgNPs and Fc-AgNPs exhibit potential for treating cancer and diseases caused by pathogenic microorganisms.","PeriodicalId":55366,"journal":{"name":"Biology Bulletin","volume":null,"pages":null},"PeriodicalIF":0.5,"publicationDate":"2024-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141195809","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Phloretin Ameliorates against Bisphenol A Induced Polycystic Ovarian Syndrome, an Endocrine Disrupting Chemical, in Prepubertal Rats 植物黄酮能缓解双酚 A 诱导的青春期前大鼠多囊卵巢综合征（一种干扰内分泌的化学物质）。

IF 0.5 4区生物学

Biology Bulletin Pub Date : 2024-05-31 DOI: 10.1134/s1062359022601173

Nilufer Coşkun, Nurhayat Barlas

{"title":"Phloretin Ameliorates against Bisphenol A Induced Polycystic Ovarian Syndrome, an Endocrine Disrupting Chemical, in Prepubertal Rats","authors":"Nilufer Coşkun, Nurhayat Barlas","doi":"10.1134/s1062359022601173","DOIUrl":"https://doi.org/10.1134/s1062359022601173","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3>Polycystic ovary syndrome (PCOS) is an endocrine disorder whose etiology is not well understood and affecting 9–18% of women in reproductive age. We investigated the protective effects of phloretin against the PCOS, induced Bisphenol A (BPA) on prepubertal rat ovaries. This study started on female rats on the postnatal 28th day via subcutaneous injection by dissolving the compounds in corn oil at 30 min intervals, starting with phloretin, and followed by BPA. The dose of BPA was 50 mg/kg/day, and the doses of phloretin were 0.5, 5, and 50 mg/kg/day. Treatments were administered every day for 15 days. Likewise corn oil, BPA and testosterone propionate were given by the same way. BPA exposed groups, it has been observed that there are many cystic follicles in the ovary and their morphology has changed. Similarly, prolonged diestrus phases was observed in the smear cycle. The CYP11A1 and CYP17A enzymes stained intensively on the BPA group whereas these enzymes were stained weakly in the BPA + 5 and 50 mg/kg phloretin groups. In PCOS, induced by BPA, the cystic follicles were recorded on the ovaries whereas it was decreased in the BPA + phloretin groups. Prepubertal exposure to BPA was associated with decreased serum luteinizing hormone (LH), and follicle stimulating hormone (FSH) levels, increased estrogen (ERRα) and dihydrotestosterone (DHT) levels in testosterone propiyonat and BPA + phloretin (0.5 mg/kg/day) groups. In conclusion, in rat model, phloretin has positive effects against BPA induced polycystic ovarian syndrome, also it caused positive effects on ovarian tissue and hormone secretion.","PeriodicalId":55366,"journal":{"name":"Biology Bulletin","volume":null,"pages":null},"PeriodicalIF":0.5,"publicationDate":"2024-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141195729","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0