Biology Bulletin最新文献_第10页

Protective Effect of Echinops persicus Extract on Oxidative Stress Enzymes in Various Rat Tissues after Carbon Tetrachloride Intoxication 棘果提取物对四氯化碳中毒后大鼠各种组织中氧化应激酶的保护作用

IF 0.5 4区生物学

Biology Bulletin Pub Date : 2024-02-09 DOI: 10.1134/s1062359023602562

Maryam Soori, Mohammad Taghi Goodarzi, Hassan Mehrad-Majd, Hossein Abbaspour, Seyed Hossein Hashemi, Zeynab Soori

{"title":"Protective Effect of Echinops persicus Extract on Oxidative Stress Enzymes in Various Rat Tissues after Carbon Tetrachloride Intoxication","authors":"Maryam Soori, Mohammad Taghi Goodarzi, Hassan Mehrad-Majd, Hossein Abbaspour, Seyed Hossein Hashemi, Zeynab Soori","doi":"10.1134/s1062359023602562","DOIUrl":"https://doi.org/10.1134/s1062359023602562","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3><p>Medicinal plants have been proposed as potential therapeutic agents for the treatment and prevention of various oxidative stress-related disorders. The purpose of this study was to investigate the effect of <i>Echinops persicus</i> (<i>Ec.P</i>) extract on catalase (CAT), superoxide dismutase (SOD), and glutathione peroxidase (GPx) activities in different rat tissues after carbon tetrachloride (CCl<sub>4</sub>) intoxication. A total of 38 rats weighing 180–210 g were randomly divided into eight groups of five. Rats in group 1 received only a standard diet and served as the control group, those in group 2 were given olive oil, and the rats in group 3 received olive oil + CCl<sub>4</sub>. Groups 4 to 8 were experimental groups. Rats of the experimental groups received olive oil + CCl<sub>4</sub> in a ratio of 1 : 1 v/v daily for 7 days. After this period, vitamins C and E were given in 10 mg/kg doses to groups 4 and 5, respectively. Rats of groups 6, 7, and 8 were also treated with 0.5, 1, and 2 g/kg body weight of <i>Ec.P</i> extract, respectively, for the next seven days. The kidney, liver, and heart supernatants of all rats were used to measure enzyme activities. Results: Decreased activity of SOD, CAT, and GPx were found in the liver, kidney, and heart after CCl<sub>4</sub> intoxication. The aqueous extract of <i>Ec.P</i> showed a significant protective effect by elevating antioxidant enzyme activities, compared to vitamins C and E in a dose and tissue-dependent manner. The obtained results of the current study highlighted the protective effect of <i>Ec.P</i> extract on antioxidant enzyme activities. The <i>Ec.P</i> could probably be significant in treating oxidative stress-related disorders.</p>","PeriodicalId":55366,"journal":{"name":"Biology Bulletin","volume":"30 1","pages":""},"PeriodicalIF":0.5,"publicationDate":"2024-02-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139766839","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Multivariate Spatial Structure of Secondary Forests in Tuyen Quang Province, Vietnam 越南敦广省次生林的多元空间结构

IF 0.5 4区生物学

Biology Bulletin Pub Date : 2024-02-09 DOI: 10.1134/s1062359023603932

{"title":"Multivariate Spatial Structure of Secondary Forests in Tuyen Quang Province, Vietnam","authors":"","doi":"10.1134/s1062359023603932","DOIUrl":"https://doi.org/10.1134/s1062359023603932","url":null,"abstract":"<span> <h3>Abstract</h3> <p>Forest management relies on forest spatial structure, but studies on the spatial structure of Vietnamese forests are limited. Therefore, two one–hectare plots were established in tropical rainforest in Tuyen Quang province to evaluate the characteristics and spatial structures of plant communities. Descriptive statistics and species importance value indices were calculated for each plot. Then, to assess differences between plots, detrended correspondence analysis was applied. Species mingling, uniform angle, diameter dominance and carbon stock dominance indices were also computed to analyze forest spatial structures. The results indicated that 65–70% of trees occurred in areas with high diversity, and 55–58% of trees were randomly distributed. The number of dominant and non–dominant individuals, in terms of diameter and carbon stock, was similar at the stand level. In all estimated patterns, units with high species mixtures predominated. As biodiversity increased, the distribution of forest trees became more random. The quadrivariate distribution illustrated considerable differences between plots, the study area and other regions. Findings from multivariate distributions provide accurate information in each group of spatial indices and these can be used to select appropriate silvicultural measures for stand management.</p> </span>","PeriodicalId":55366,"journal":{"name":"Biology Bulletin","volume":"97 1","pages":""},"PeriodicalIF":0.5,"publicationDate":"2024-02-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139771402","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

HMGB1 May Modulates Proliferation and Migration of Endothelial Cells by YAP1 under Hypoxia 缺氧条件下，HMGB1 可通过 YAP1 调节内皮细胞的增殖和迁移

IF 0.5 4区生物学

Biology Bulletin Pub Date : 2024-02-09 DOI: 10.1134/s1062359023602331

Liu Weiwei, Wang Yan, Li Jiao, Han Weiyu, Zhao Ranzun, Shi Bei

{"title":"HMGB1 May Modulates Proliferation and Migration of Endothelial Cells by YAP1 under Hypoxia","authors":"Liu Weiwei, Wang Yan, Li Jiao, Han Weiyu, Zhao Ranzun, Shi Bei","doi":"10.1134/s1062359023602331","DOIUrl":"https://doi.org/10.1134/s1062359023602331","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3><p>Endothelial dysfunction is a key factor in the occurrence of coronary artery disease and plays a vital role in the pathological development of myocardial infarction. Hypoxia contributing to endothelial cell survival and growth, metabolism and angiogenesis. HMGB1 has been identified as one of the factors involved in the regulation of vascular endothelial cell function. However, the mechanisms by which HMGB1 regulates endothelial cells under hypoxia remain unclear. Our study aimed to determine HMGB1 expression investigate the functions of HMGB1 on endothelial cell in hypoxia environment. The expression of extracellular high-mobility group box 1 (HMGB1) in cardiac endothelial cells treated with hypoxia was detected by western blot analysis. 5-Ethynyl-2′-deoxyuridine (EdU) assays and transwell assays were performed to determine the cell proliferation and migration, and flow cytometry assay was performed to evaluate the apoptosis of cardiac endothelial cell. Intracellular HMGB1 expression was upregulated in hypoxia-induced cardiac endothelial cells. HMGB1 silencing significantly promoted apoptosis and inhibited the proliferation and migration of hypoxia-induced cardiac endothelial cells. Moreover, silencing YAP1 on the basis of silencing HMGB1 can aggravate apoptosis and inhibit proliferation and migration of cardiac endothelial cells in hypoxic microenvironment. HMGB1 is up-regulated in hypoxic environment, which may be regulate the biological function of cardiac endothelial cells by YAP1, revealing that HMGB1/YAP1 may be partially involved in the protective mechanism of cardiac endothelial cells. To the best of our knowledge, this study provides a novel basis for the development of treatment methods for ischemic heart diseases.</p>","PeriodicalId":55366,"journal":{"name":"Biology Bulletin","volume":"48 5 1","pages":""},"PeriodicalIF":0.5,"publicationDate":"2024-02-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139771425","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Deviations in the Development of Captivity Walrus (Odobenus rosmarus) Skull 圈养海象（Odobenus rosmarus）头骨发育的偏差

IF 0.5 4区生物学

Biology Bulletin Pub Date : 2024-02-09 DOI: 10.1134/s106235902360321x

N. V. Kryukova, D. A. Manukhova, M. D. Slavina

{"title":"Deviations in the Development of Captivity Walrus (Odobenus rosmarus) Skull","authors":"N. V. Kryukova, D. A. Manukhova, M. D. Slavina","doi":"10.1134/s106235902360321x","DOIUrl":"https://doi.org/10.1134/s106235902360321x","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3><p>Currently, many aquariums and zoos around the world keep walruses in captivity. However, the presence of tusks in walruses creates difficulties in their maintenance and preservation of their health since they wear them down on the structure of the pools. In this study, the morphometric parameters of walrus skulls from captivity and wild of similar age were examined (35 skull measurements and 21 mandible measurements were taken). An examination of the skulls of three female walruses that were kept in the Moscow Zoo for 8, 11, and 22 years, respectively during the period of 1953–2005 was conducted. The tusks of one walrus, at the age of 4 years, were surgically removed, while the tusks of the others were not. There were slight deviations in the skull development of these walruses. The skull of captive walruses is slightly smaller in the mastoid processes and zygomatic arches, and facial part, and the lower jaw is smaller in width and symphysis circumference compared to wild walruses. The surface of the skull and lower jaw is smoother in comparison to wild walruses. Diseases such as odontogenic osteomyelitis, pulpitis, periodontitis, dental calculus and caries are observed. Due to frequent inflammations and surgical interventions, early fusion of sutures is noted, leading to skull deformities.</p>","PeriodicalId":55366,"journal":{"name":"Biology Bulletin","volume":"1 1","pages":""},"PeriodicalIF":0.5,"publicationDate":"2024-02-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139771437","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In Vitro Cytotoxic, Genotoxic, Embryotoxic and Oxidative Damage Potentials by Empagliflozin 恩格列净的体外细胞毒性、基因毒性、胚胎毒性和氧化损伤潜力

IF 0.5 4区生物学

Biology Bulletin Pub Date : 2024-02-09 DOI: 10.1134/s1062359023603919

Kenan Cadirci, Hasan Turkez, Ozlem Ozdemir Tozlu, Omer Erkan Yapca, Muharrem Bayrak, Bugrahan Emsen, Adil Mardinoglu

{"title":"In Vitro Cytotoxic, Genotoxic, Embryotoxic and Oxidative Damage Potentials by Empagliflozin","authors":"Kenan Cadirci, Hasan Turkez, Ozlem Ozdemir Tozlu, Omer Erkan Yapca, Muharrem Bayrak, Bugrahan Emsen, Adil Mardinoglu","doi":"10.1134/s1062359023603919","DOIUrl":"https://doi.org/10.1134/s1062359023603919","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3><p>Empagliflozin (EMPA) is a potent, competitive and selective sodium glucose cotransporter-2 (SGLT-2) inhibitor that ameliorates blood glucose with the insulin-independent manner. EMPA reduces weight and blood pressure of patients with type 2 diabetes mellitus (T2DM) without developing hypoglycemic risk. To the best of our knowledge, its safety profiling has not been evaluated on human blood cell cultures yet. Again, the embryotoxicity potential by EMPA is still unclear. Therefore, in this investigation we aimed to evaluate the in vitro cytotoxic, genotoxic and embryotoxic damage potential as well as antioxidative/oxidative effects by EMPA in cultured human blood and human pluripotent embryonal carcinoma NT2 cells for the first time. Cell cultures (<i>n</i> = 5) were exposed to different concentrations ranging from 3.25 to 100 mg/L of EMPA for 48 and 72 h. Cell viability was measured by using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and lactate dehydrogenase (LDH) release assays. The alterations in antioxidant/oxidant activity were monitored via measuring the total antioxidant capacity (TAC) and total oxidative stress (TOS) levels. For evaluating the genotoxicity of EMPA chromosomal aberration (CA) assay was performed. The present results revealed that EMPA did not induce cytotoxic or genotoxic damage on healthy human blood cells. Moreover, EMPA exerted non-embryotoxic property and supported antioxidative capacity and decreased the oxidative stress in cultured human blood cells. Our results supported the safe and advantageous use of EMPA for the treatment of T2DM.</p>","PeriodicalId":55366,"journal":{"name":"Biology Bulletin","volume":"15 1","pages":""},"PeriodicalIF":0.5,"publicationDate":"2024-02-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139771411","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Intraspecific Variability of Quercus pubescens Willd. Leaves from the Outstanding Natural Landscape “Kosmaj” in Serbia 塞尔维亚 "科斯马伊 "杰出自然景观中栎树叶片的种内变异性

IF 0.5 4区生物学

Biology Bulletin Pub Date : 2024-02-09 DOI: 10.1134/s1062359023603877

M. Jovanović, I. Kerkez Janković, J. Milovanović, M. Nonić, M. Šijačić-Nikolić

引用次数: 0

Investigation of the Lactic Acid Bacteria in Different Traditional Cheeses of Azerbaijan 调查阿塞拜疆不同传统奶酪中的乳酸菌

IF 0.5 4区生物学

Biology Bulletin Pub Date : 2024-02-09 DOI: 10.1134/s1062359023603427

{"title":"Investigation of the Lactic Acid Bacteria in Different Traditional Cheeses of Azerbaijan","authors":"","doi":"10.1134/s1062359023603427","DOIUrl":"https://doi.org/10.1134/s1062359023603427","url":null,"abstract":"<span> <h3>Abstract</h3> <p>Lactic acid bacteria (LAB) are probiotic bacteria that have many health benefits. The aim of study was to determinate LAB from traditional cheeses (Gedebey, Ivanovka, Karabakh) consumed in Azerbaijan. The microbial content and loading of three traditional samples were explored using counting methods and a culture-independent approach. The average count of total bacteria in both of samples ranged between 10<sup>5</sup> and 10<sup>7</sup> cfu/g. In all samples, aerobic bacteria and lactic acid bacteria were more >4.9 × 10<sup>5</sup> cfu/g. The V3-V4 region of 16S rDNA was amplified from total DNA extracted from traditional cheese samples and microbial communities and lactic acid bacteria were determined. In general, the diversity of microbial populations in cheese samples were <em>Lactobacillus</em>, <em>Lactococcus</em>, <em>Streptococcus</em>. Among the three samples, Gedebey sample showed the highest richness. Obtained data can be use in the food industry. In addition, lactic acid bacteria analysis could help the determination of potential strains for traditional cheese production. In conclusion, determination and identification of LAB strains in traditional cheeses will reveal the traditional food microbiota and will help to find potential probiotic bacteria for health and industry.</p> </span>","PeriodicalId":55366,"journal":{"name":"Biology Bulletin","volume":"21 1","pages":""},"PeriodicalIF":0.5,"publicationDate":"2024-02-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139771432","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Floral Polychromatism and Female Fitness in Hermaphrodites of Garcinia gummi-gutta (L.) Roxb. 雌雄同体的花多色性和雌性体质

IF 0.5 4区生物学

Biology Bulletin Pub Date : 2024-01-18 DOI: 10.1134/s1062359023600332

{"title":"Floral Polychromatism and Female Fitness in Hermaphrodites of Garcinia gummi-gutta (L.) Roxb.","authors":"","doi":"10.1134/s1062359023600332","DOIUrl":"https://doi.org/10.1134/s1062359023600332","url":null,"abstract":"<span> <h3>Abstract</h3> <p><em>Garcinia gummi-gutta</em> (L.) Roxb. is a tropical tree crop widely gaining attention due to the plenteousness of hydroxycitric acid (HCA), known for its anti-obesity and appetite-reducing activity. The declining male population, coupled with sexual complexity and floral diversity in fruit bearer, are challenging for natural pollination and hence require conservation of the germplasm. The investigation in this study focused on reproductive characterization, resource allocation, reproductive success, and male functionality could better explain the existing sexual system and its mode of reproduction. Great diversity in floral color of <em>G. gummi-gutta</em> specimens ranging from sulphur-yellow to crimson red in hermaphrodite compared to yellow-shaded male flowers was noticed in South Western Ghats. Analysis of variance (ANOVA) revealed significant variations in floral quantitative characters across the sexual forms. However, no direct association was evident with reproductive success explicitly across male and hermaphrodite flowers. The Student’s <em>t</em>-test among hermaphrodite and male sex morphs unveiled a significant mean difference in pollen germination, and pollination was further defined with correlation analysis. The negative association of pollen germination with the female trait, viz., seed production, shows a trade-off to female reproductive fitness in hermaphrodite flowers. The androdiecious nature of the species is agreed upon the presence of fertile pollen grains by the hermaphrodite trees, and the report is the first of our knowledge.</p> </span>","PeriodicalId":55366,"journal":{"name":"Biology Bulletin","volume":"198 1","pages":""},"PeriodicalIF":0.5,"publicationDate":"2024-01-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139507687","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of Interactions of Triterpenes in M. charantia with Proteins Involved in Vascularization in In Silico 三萜类化合物与参与血管形成的蛋白质相互作用的硅学评估

IF 0.5 4区生物学

Biology Bulletin Pub Date : 2024-01-18 DOI: 10.1134/s1062359022602646

B. Sevim, O. Eroğlu

{"title":"Evaluation of Interactions of Triterpenes in M. charantia with Proteins Involved in Vascularization in In Silico","authors":"B. Sevim, O. Eroğlu","doi":"10.1134/s1062359022602646","DOIUrl":"https://doi.org/10.1134/s1062359022602646","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3><p>Angiogenesis is an important process that plays an active role in tumorigenesis. VEGFRs, a member of the tyrosine kinase receptor family involved in this process, is known as the receptor for VEGF ligands in tumor cells. c-Src is an adapter protein located downstream of VEGFRs and plays a role in angiogenic signaling. SPARC protein has recently been shown to play a role in metastasis in various types of cancer. In this study, inhibition of angiogenesis via extracellular matrix and VEGF/VEGFRs is aimed. <i>Momordica charantia</i>; is a valuable plant used quite often in traditional medicine. Triterpenes from various regions of plant appear to be promising in in vitro cancer-related studies. In our study; literature was searched to identify possible triterpenes in this plant; triterpenes in fruit and seed were selected. The 2D and 3D structure files of these triterpenes were obtained from PubChem. The structure files of the ligands were prepared with various programs and converted to the appropriate file format. X-ray diffraction structures of proteins were obtained from RCSB PDB. These structure files were made suitable for molecular docking studies. Docking and scoring were performed with the Vina program to select the appropriate poses. According to the <i>in silico</i> analysis; It has been found that various triterpenes that can be obtained from <i>M. charantia</i> plant may inhibit VEGFRs, SPARC, and c-Src proteins. These results show that these triterpenes are promising in terms of new natural therapeutic routes and drug candidates for aggressive cancer therapy.</p>","PeriodicalId":55366,"journal":{"name":"Biology Bulletin","volume":"108 1","pages":""},"PeriodicalIF":0.5,"publicationDate":"2024-01-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139507697","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Influence of Bio-AgNP on Growth and Biochemical Composition of Spirulina platensis 生物氮化镁对螺旋藻生长和生化成分的影响

IF 0.5 4区生物学

Biology Bulletin Pub Date : 2024-01-18 DOI: 10.1134/s1062359023601015

P. Nartop, E. Kuşku

引用次数: 0