Biology BulletinPub Date : 2024-09-10DOI: 10.1134/s1062359024607432
O. E. Mazur, I. A. Kutyrev, L. V. Tolochko
{"title":"Leukocyte Composition of the Organs of the Immune System of Coregonus migratorius Infected with Dibothriocephalus dendriticus","authors":"O. E. Mazur, I. A. Kutyrev, L. V. Tolochko","doi":"10.1134/s1062359024607432","DOIUrl":"https://doi.org/10.1134/s1062359024607432","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3><p>For the first time, an analysis of leukocyte changes in the organs of the immune system (pronephros, mesonephros, and spleen) of the Baikal omul <i>Coregonus migratorius</i> (Georgi, 1775) (Salmoniformes: Coregonidae) infected with <i>Dibothriocephalus dendriticus</i> (Nitsch, 1824) (syn. <i>Diphyllobothrium dendriticum</i>) (Cestoda: Diphyllobothriidea) is presented in natural habitat conditions. In the pronephros of infected fish, the number of poorly differentiated neutrophils (myelocytes and metamyelocytes) was significantly higher than in uninfected fish, which indicated inflammatory processes. <i>D. dendriticus</i> triggered the immunoregulatory processes in the body of infected fish, as evidenced by the decrease in the total number of lymphocytes and prolymphocytes (B-lymphocytes) in the pronephros and the increase in prolymphocytes in the spleen. The other effector elements (cells of the monocyte–macrophage lineage, basophils, and eosinophils) were barely involved in antiparasitic defense.</p>","PeriodicalId":55366,"journal":{"name":"Biology Bulletin","volume":"23 1","pages":""},"PeriodicalIF":0.5,"publicationDate":"2024-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142187678","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Biology BulletinPub Date : 2024-09-10DOI: 10.1134/s1062359024607444
O. V. Chubukova, L. R. Khakimova, R. T. Matnyazov, Z. R. Vershinina
{"title":"Heavy Metal-Resistant PGPR Strains of Pseudomonas sp. Stimulating the Growth of Alfalfa under Cadmium Stress","authors":"O. V. Chubukova, L. R. Khakimova, R. T. Matnyazov, Z. R. Vershinina","doi":"10.1134/s1062359024607444","DOIUrl":"https://doi.org/10.1134/s1062359024607444","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3><p>Three bacteria strains of <i>Pseudomonas</i> sp. resistant to heavy metals were isolated from chemically contaminated soil. An analysis of the nucleotide sequences of the 16S rRNA and <i>rpoD</i> genes identified the <i>Pseudomonas</i> sp. 17 НМ strain as <i>Pseudomonas capeferrum,</i> and the strains of <i>Pseudomonas</i> sp. 65 НМ and 67 НМ were most closely related to the type strains of <i>Pseudomonas silesiensis</i> and <i>Pseudomonas umsongensis</i>, respectively. It was shown that the strains of <i>Pseudomonas</i> sp. 17 НМ, 65 НМ, and 67 НМ were characterized by different levels of resistance to heavy metals, with the maximum tolerable concentration (MTC) of zinc being 1 mМ for all strains, that of cadmium, 1, 1.5, and 1 mМ; of lead, 5, 5, and 4 mМ; and of nickel, 7, 9, and 7 mМ, respectively. All <i>Pseudomonas</i> strains can form biofilms and have the properties of PGPR bacteria. Treatment of alfalfa seeds (<i>Medicago sativa</i> L.) with the strains of <i>Pseudomonas</i> sp. 17 НМ, 65 НМ, and 67 НМ under cadmium stress increased the dry weight of alfalfa seedlings up to 40% and the content of chlorophyll <i>a</i> and <i>b</i> in the leaves by 25–33% relative to the control.</p>","PeriodicalId":55366,"journal":{"name":"Biology Bulletin","volume":"59 1","pages":""},"PeriodicalIF":0.5,"publicationDate":"2024-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142187796","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Biology BulletinPub Date : 2024-09-10DOI: 10.1134/s1062359024608206
E. I. Naumova, G. K. Zharova, T. Yu. Chistova
{"title":"Dynamics of Food Passing through the Digestive Tract in the Nocturnal Rodent Meriones crassus as a Response to the Rhythm of Feeding Activity","authors":"E. I. Naumova, G. K. Zharova, T. Yu. Chistova","doi":"10.1134/s1062359024608206","DOIUrl":"https://doi.org/10.1134/s1062359024608206","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3><p>The dependence of the food passing through the digestive tract (DT) on feeding activity in the nocturnal rodent <i>Meriones crassus</i> was studied. Two groups of gerbils were housed in the laboratory under artificial lighting at a photoperiod of 12 : 12 L : D for 36 h. The groups were named “day” (L : D = 20 : 16) and “night” (L : D = 12 : 24) according to the predominance light or dark time during the experiment. The mean retention time of markers in the stomach in these groups was 20 and 30 h, respectively. The kinetics of the contents changed depending on the specific time of consumption of the markers and the saturation level of the rodents with food. During the daylight hours, sequential evacuation of markers entering the DT begins from the stomach after two hours, and at night, during a period of higher feeding activity, part of the food located in the fornix of the stomach and in the cecum stays here for a longer time. In the “day” group, by the end of the experiment, 84% of the markers were removed from the DT, and in the “night” group, about 55%.</p>","PeriodicalId":55366,"journal":{"name":"Biology Bulletin","volume":"8 1","pages":""},"PeriodicalIF":0.5,"publicationDate":"2024-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142188025","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Biology BulletinPub Date : 2024-09-10DOI: 10.1134/s1062359024607924
N. N. Kruglova, I. R. Galin, N. A. Yegorova
{"title":"The Content of Endogenous Hormones in Explants and Calluses of Lavandula angustifolia Mill. at the Initial Stages of an In Vitro Culture","authors":"N. N. Kruglova, I. R. Galin, N. A. Yegorova","doi":"10.1134/s1062359024607924","DOIUrl":"https://doi.org/10.1134/s1062359024607924","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3><p>The content of endogenous hormones (auxin IAA, cytokinines, ABA) in explants of various types (segments of the leaf, bud, and stem), primary calluses induced from them, and morphogenic and nonmorphogenic calluses at the initial stages of in vitro culture by the immunoassay method was studied for the first time for <i>Lavandula angustifolia</i> Mill. The maximum value of the hormone levels in explants such as segments of a bud was shown. An increase in the content of hormones in primary calluses was revealed in comparison with similar characteristics in all types of explants. The higher level of the active form of cytokinin (<i>trans</i>-zeatin) and ABA, as well as the lower level of the inactive form of cytokinin (zeatin-nucleotide) and auxin IAA, were identified in the morphogenic callus compared with the nonmorphogenic one. It is suggested that the content of endogenous hormones in explants and calluses of <i>L. angustifolia</i> is due to their histological status. A conclusion is made about the unified histophysiological mechanisms of callusogenesis and morphogenesis in vitro in the studied plant.</p>","PeriodicalId":55366,"journal":{"name":"Biology Bulletin","volume":"9 1","pages":""},"PeriodicalIF":0.5,"publicationDate":"2024-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142187794","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Biology BulletinPub Date : 2024-09-10DOI: 10.1134/s1062359023606687
D. N. Fedorin, A. T. Eprintsev
{"title":"Molecular Aspects of Regulation of the Expression of Homologic Glutamate Dehydrogenase Genes in Wheat Leaves under Hypoxia","authors":"D. N. Fedorin, A. T. Eprintsev","doi":"10.1134/s1062359023606687","DOIUrl":"https://doi.org/10.1134/s1062359023606687","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3><p>An increase in GDH activity has been established in the first six hours after plants are exposed to hypoxic conditions, which ensures the formation of an adaptive response of cellular metabolism to the lack of oxygen in the cell. A difference in the transcriptional activity of homologous <i>GDH-1</i> genes in wheat leaves under stress conditions was shown. An increase in the mRNA content of the <i>GDH-1</i>(<i>5A</i>) gene in wheat leaves after plants are exposed to hypoxic conditions is observed in the first hours of the experiment, which correlates with a change in the catalytic activity of glutamate dehydrogenase. Regulation of this gene is carried out by the transcription factor HIF, the specific landing site of which is found in the transcription initiation site of the promoter of this gene.</p>","PeriodicalId":55366,"journal":{"name":"Biology Bulletin","volume":"12 1","pages":""},"PeriodicalIF":0.5,"publicationDate":"2024-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142187798","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Phylogenetic Inference from 16S rRNA Gene Sequencing in Chromosome Races of the Genus Nannospalax Species (Rodentia: Spalacidae)","authors":"Teoman Kankılıç, Habibe Didem Çelikbilek, Tolga Kankılıç, Perinçek Seçkinozan Şeker, Engin Selvi, İlkay Civelek","doi":"10.1134/s1062359023605281","DOIUrl":"https://doi.org/10.1134/s1062359023605281","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3><p>In this study, we aimed to develop a species-level phylogeny for the <i>Nannospalax</i> genus, identify cryptic species that are difficult to differentiate using standard methods, and further evaluate the relationships across chromosomal forms of several species. <i>16S rRNA</i> gene sequences from 81 Turkish regions were evaluated and statistical analyzes were conducted. Results are as follows; (i) <i>Nannospalax cilicicus</i> was found to be monophyletic and sister in relation to <i>Nannospalax xanthodon</i>; (ii) We suggested for the first time that the Central-Anatolian populations should be classified under the species name <i>Nannospalax cilicicus</i> (stat. n.); (iii) the unidentified 2<i>n</i> = 52 cytotypes from Bolu and <i>Nannospalax leucodon</i> cytotypes were reciprocally monophyletic, these data support the notion that the 2<i>n</i> = 52 cytotypes from Bolu belong to an unidentified taxon; (iv) study also offered evidence for the monophyly of <i>N. tuncelicus</i> for the first time; (v) <i>Nannospalax xanthodon</i> (2<i>n</i> = 36, 38, and 40) and <i>Nannospalax nehringi</i> composed several species-specific clades, which form polytomy structure, so relationships between these species are still unclear.</p>","PeriodicalId":55366,"journal":{"name":"Biology Bulletin","volume":"39 1","pages":""},"PeriodicalIF":0.5,"publicationDate":"2024-07-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141609073","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Biology BulletinPub Date : 2024-07-13DOI: 10.1134/s1062359024607699
Archana Bachheti, Pratibha Naithani, Ashish Kumar Arya, Kamal Kant Joshi
{"title":"Avian Diversity: A Comprehensive Bird Checklist of Graphic Era (Deemed to be University), Dehradun (Western Himalaya), India","authors":"Archana Bachheti, Pratibha Naithani, Ashish Kumar Arya, Kamal Kant Joshi","doi":"10.1134/s1062359024607699","DOIUrl":"https://doi.org/10.1134/s1062359024607699","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3><p>The present study aims to provide a comprehensive bird checklist of Graphic Era (Deemed to be University), Dehradun, India. The study was conducted in from April 2020–March 2022. A total of 85 bird species belonging to 43 families were observed during the study period. The most abundant families were Motacillidae with Six species, followed by Cisticolidae, Columbidae, Muscicapidae, Sturnidae with five species each. Out of total 73 residents and 10 winters migratory and 2 summer migratory species were identified. The percentage of resident, winter visitor and Summer Migratory avian species was found to be 85.58, 11.76 and 2.35% respectively. The Analysis of RDi (relative diversity) results indicated that the family Motacillidae was the most diverse family, with the highest RDi value of 7.1. Total three avian species namely Red-vented bulbul (<i>Pycnonotus cafer</i>) and Rock Pigeons (<i>Columba livia</i>) and Rose-ringed parakeet (<i>Psittacula krameri</i>) were dominantly spared in the University Campus. One species Alexandrine Parakeet (<i>Psittacula eupatria</i>) which observed during the study falls under the Near Threatened (NT) category. The results of the study reflect the importance of such areas as important bird habitats where various local and migratory birds are found to rest and feed.</p>","PeriodicalId":55366,"journal":{"name":"Biology Bulletin","volume":"48 1","pages":""},"PeriodicalIF":0.5,"publicationDate":"2024-07-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141609217","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Biology BulletinPub Date : 2024-07-13DOI: 10.1134/s1062359024608401
D. Nihal, K. V. Aneesh Kumar, P. Saha, M. P. Rajeeshkumar, H. Manjebrayakath
{"title":"Growth Characteristics of 10 Deep-Sea Demersal Scorpaenoid Fish Species from the Arabian Sea: Insights for Database Update and Fisheries Management","authors":"D. Nihal, K. V. Aneesh Kumar, P. Saha, M. P. Rajeeshkumar, H. Manjebrayakath","doi":"10.1134/s1062359024608401","DOIUrl":"https://doi.org/10.1134/s1062359024608401","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3><p>The present study provides length-weight relationships (LWRs) and condition factors (Kn) estimates for ten deep-sea scorpaeniform fishes based on data consisting of new maximum size. The samples were collected from Sakthikulangara Harbour, located on the southwest coast of India, from September 2021 to December 2022. These samples were obtained as bycatch from deep-sea shrimp trawlers operating in the Arabian Sea at depths ranging from 200 to 420 m. The estimated <i>b</i> values ranged from 2.92 (<i>Brachypterois serrulata</i>) to 3.76 (<i>Pterygotrigla arabica</i>). The Kn ranged from 0.56 (<i>Satyrichthys laticeps</i>) to 1.53 (<i>Neomerinthe erostris</i>), with four species exhibiting Kn values higher than 1. The LWR estimates and Kn provided in this study are helpful to understand the growth characteristics of these data limited species and also to revalidate the modeled growth parameters using the Bayesian approach.</p>","PeriodicalId":55366,"journal":{"name":"Biology Bulletin","volume":"20 1","pages":""},"PeriodicalIF":0.5,"publicationDate":"2024-07-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141609215","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Biology BulletinPub Date : 2024-07-13DOI: 10.1134/s1062359024607171
Faouzi Horchani, Amal Bouallegue, Ahmed Namsi, Zouhaier Abbes
{"title":"Simultaneous Application of Ascorbic Acid and Proline as a Smart Approach to Mitigate the Adverse Effects of Salt Stress in Wheat (Triticum aestivum)","authors":"Faouzi Horchani, Amal Bouallegue, Ahmed Namsi, Zouhaier Abbes","doi":"10.1134/s1062359024607171","DOIUrl":"https://doi.org/10.1134/s1062359024607171","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3><p>The effects of exogenously applied ascorbic acid (AsA, 1 mM) and proline (Pro, 1 mM), were evaluated on the growth, nutritional behavior, some key biochemical attributes, oxidative damage indicators, osmolytes accumulation as well as antioxidant system in wheat seedlings (<i>Triticum aestivum</i> cv. Salammbô) grown in the presence of 100 mM NaCl. AsA or Pro supplementation ameliorated wheat seedlings’ growth and increased parameters that were reduced by salinity. Relative water content, total chlorophyll, K<sup>+</sup> and total carbohydrates contents as well as K<sup>+</sup>/Na<sup>+</sup> ratio and root-to-shoot K<sup>+</sup> translocation were significantly increased. Osmoprotectant contents were remarkably accumulated, whereas Na<sup>+</sup> content and root-to-shoot Na<sup>+</sup> translocation were significantly declined. Additionally, exogenous AsA or Pro application enhanced the antioxidant defense system components and insured better oxidative stress tolerance, as indicated by reduced H<sub>2</sub>O<sub>2</sub> production and lipid peroxidation, resulting in an increased membrane stability index. Such effects were further enhanced following the simultaneous application of AsA and Pro, resulting in a better growth, as compared to single applications of these substances. Taken together, findings of this study provide strong arguments highlighting the potential synergy between exogenously applied AsA and Pro in mitigating the adverse effects of salt stress of wheat plants at the early seedling stage.</p>","PeriodicalId":55366,"journal":{"name":"Biology Bulletin","volume":"40 1","pages":""},"PeriodicalIF":0.5,"publicationDate":"2024-07-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141609078","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Unveiling the Role of Angiosperm Pollen Morphology in Attachment to the Moth (Lepidoptera) Proboscides","authors":"Dipayan Mitra, Rajesh Lenka, Monoranjan Chowdhury, Navneet Singh","doi":"10.1134/s1062359024608322","DOIUrl":"https://doi.org/10.1134/s1062359024608322","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3><p>The current study aims to understand the role of various morphological features of pollen grains in attachment with moth proboscides. To test the hypothesis, moth samples are collected from 11 sites in three districts of Arunachal Pradesh and West Bengal, North East India. Pollen of 18 angiosperm families are isolated from the proboscides of 16 moth species in four families. We selected proboscides for the isolation of pollen because it is the most important organ of moths which interacts with flowers during moth visitation. The isolated pollen is studied for various morphological characters like pollen shape, unit, exine ornamentation, thickness, aperture numbers, and aperture types along with specialized structures i.e., viscin thread and pollenkitt, and analyzed for their attachment on the proboscides. The present study revealed the significant role of pollen shape, unit, exine characters, viscin thread, and pollenkitt in attachment with proboscides. Whereas, the aperture numbers and aperture types are found insignificant in the process of pollen attachment. Studies on the formation of viscin thread and pollenkitt may be established as an important tool to understand the specialized and generalized interactions between moths and angiosperms. The present study will open up a new horizon in pollination ecology.</p>","PeriodicalId":55366,"journal":{"name":"Biology Bulletin","volume":"2010 1","pages":""},"PeriodicalIF":0.5,"publicationDate":"2024-07-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141609214","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}