Biopreservation and Biobanking最新文献

{"title":"ISBER 2025 Annual Meeting and Exhibits: Celebrating the Impact of Biobanking Worldwide!","authors":"Daniel Catchpoole, Anusha Hettiaratchi, Vanessa Fonseca Tumilasci","doi":"10.1089/bio.2025.0019","DOIUrl":"10.1089/bio.2025.0019","url":null,"abstract":"","PeriodicalId":55358,"journal":{"name":"Biopreservation and Biobanking","volume":" ","pages":"65-66"},"PeriodicalIF":1.6,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143071253","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Window into Australasian Biobanking-Showcasing Innovation, Highlighting Conservation, and Embracing Diversity.","authors":"Cassandra P Griffin, Anusha Hettiaratchi, Georget Reaiche-Miller, Alison Parry Jones","doi":"10.1089/bio.2025.0014","DOIUrl":"10.1089/bio.2025.0014","url":null,"abstract":"","PeriodicalId":55358,"journal":{"name":"Biopreservation and Biobanking","volume":" ","pages":"1-2"},"PeriodicalIF":1.6,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143071250","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Framing a Biobanking Response to Myrtle Rust in Western Australia.","authors":"Emma L Dalziell, Bryn Funnekotter, Matthew D Barrett, Alyssa M Martino, Amanda Shade, Matthew Stray, David J Merritt","doi":"10.1089/bio.2024.0098","DOIUrl":"10.1089/bio.2024.0098","url":null,"abstract":"<p><p>Myrtle rust is a plant disease caused through infection by the fungus <i>Austropuccinia psidii</i> and was first detected in Australia in 2010. The disease has spread through New South Wales, Victoria, Queensland, the Northern Territory, and Tasmania. In this short timeframe, myrtle rust has had a devastating impact on many native species in the family Myrtaceae, including several rainforest species that are now at risk of extinction. In 2022, myrtle rust was first detected in the northern part of Western Australia (WA)-the largest state in Australia. WA is home to <i>ca.</i> 2000 Myrtaceae taxa (<i>ca.</i> 60% of Australia's Myrtaceae diversity), many of which form the dominant component of the vegetation across several ecosystems (e.g., <i>Eucalyptus, Corymbia, Melaleuca, Agonis, Verticordia</i> etc.). While modelling suggests that the environmental conditions in WA's north are less conducive to myrtle rust in comparison to the wet, temperate rainforests of the east coast, WA's temperate, Myrtaceae-rich south coast may be climatically suitable. Coupled with the sheer abundance of Myrtaceae species in WA, their high degree of endemism, high proportion of threatened species, and little available information on their susceptibility to myrtle rust, a pre-emptive strategy to conserve germplasm of at-risk species is warranted. This paper highlights the role of <i>ex situ</i> germplasm conservation in responding to biosecurity threats such as myrtle rust. With early intervention critical to sourcing healthy and genetically diverse germplasm, we present a prioritized list of genera and species of Myrtaceae in WA to inform strategic, coordinated, and timely <i>ex situ</i> conservation actions, along with case studies to illustrate the complementary approaches of seed banking, cryobiotechnology, and tissue culture necessary to conserve germplasm of WA's myrtaceous flora.</p>","PeriodicalId":55358,"journal":{"name":"Biopreservation and Biobanking","volume":" ","pages":"11-22"},"PeriodicalIF":1.6,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142717423","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bolstering Buck Fertility: The Impact of <i>Asparagus racemosus</i> Aqueous Extract on Semen Cryopreservation and Antioxidant Defense System.","authors":"Chetna Gangwar, Ashok Kumar, K Gururaj, Anshuman Kumar, Salauddin Qureshi, Manish Kumar, Anil Kumar Mishra, R Ranjan","doi":"10.1089/bio.2023.0117","DOIUrl":"10.1089/bio.2023.0117","url":null,"abstract":"<p><p><b><i>Importance of Study:</i></b> Semen cryopreservation results in sperm damage due to lipid peroxidation or oxidative stress, leading to a decrease in conception rate. The sperm damage during cryopreservation can be minimized with the use of suitable antioxidant supplements in semen diluent. Some herbs have potent antioxidant potential and can be used in semen diluent to protect the spermatozoa. <b><i>Objective:</i></b> Hence, the investigation was planned to evaluate the effect of <i>Asparagus racemosus</i> (<i>A. racemosus</i>) aqueous extract on buck semen quality during cryopreservation. <b><i>Methodology:</i></b> In the current study, semen was collected from eight Sirohi bucks, and from each buck, 8 ejaculates were collected. Good-quality semen samples were pooled during each collection. Pooled semen samples were then divided into four equal parts and diluted in TRIS buffer containing different concentrations of <i>A. racemosus</i> aqueous extract (different groups, i.e., G I -5 mg, G II -2.5 mg, G III -1.25 mg, and G IV -0 mg of <i>A. racemosus</i> aqueous extract in 1 mL TRIS buffer). All the diluted semen samples were kept at equilibration temperature (5°C) for 2 hours and then cryopreserved by the manual method. Semen samples were evaluated for various sperm characteristics and antioxidant status before and after cryopreservation. <b><i>Results:</i></b> <i>Asparagus racemosus</i> aqueous extract showed significant (<i>p</i> < 0.05) enhancement of sperm viability, sperm motility, acrosomal integrity, and plasma membrane integrity, whereas it reduced sperm abnormality. Furthermore, in the experimental groups, the antioxidant gene expression was found to be increased compared to that of the treatment group. G III (<i>p</i> < 0.05) showed significantly better results in terms of sperm viability, sperm motility, acrosomal integrity, and plasma membrane integrity. <b><i>Conclusion:</i></b> <i>Asparagus racemosus</i> aqueous extract has the antioxidant potential to protect buck spermatozoa during semen cryopreservation.</p>","PeriodicalId":55358,"journal":{"name":"Biopreservation and Biobanking","volume":" ","pages":"53-61"},"PeriodicalIF":1.6,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141199975","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cryoprotective Property of Ethylene Glycol in Regard to the Quality and Mitochondrial Status of Frozen Indian Red Jungle Fowl (<i>Gallus Gallus Murghi</i>) Semen.","authors":"Fiza Khursheed, Bushra Allah Rakha, Sumiyyah Zuha, Muhammad Sajjad Ansari, Shamim Akhter","doi":"10.1089/bio.2024.0063","DOIUrl":"https://doi.org/10.1089/bio.2024.0063","url":null,"abstract":"<p><p><b><i>Aim:</i></b> Ethylene glycol (EG) has been employed as a cryoprotectant for many years in mammalian semen cryopreservation but not assessed for birds except for its recently illustrated beneficial effects on commercial chicken lines. The Indian red jungle fowl is facing trouble in its native range due to human encroachment. Therefore, the present study was designed to elucidate the cryoprotective effect of different EG concentrations (5%, 10%, 15%, and 20%) on frozen Indian red jungle fowl semen. <b><i>Materials and Methods:</i></b> Semen was collected from 20 cocks, and qualifying ejaculates (>70% motility) were pooled and diluted (15) with red fowl extender. EG was added to the four samples and 20% glycerol in control at 4°C. Samples were equilibrated and cryopreserved in LN₂. Semen quality and biochemical activity were assessed at various stages of cryopreservation. <b><i>Results:</i></b> Sperm motility, viability, plasma membrane and acrosomal integrity, chromatin integrity, and mitochondrial activity were recorded highest (<i>p</i> < 0.05) with 20% EG at the post-equilibration and post-thaw stages. Lipid peroxidation was recorded lowest (<i>p</i> < 0.05) with 20% EG compared with other concentrations and control at the post-equilibration and post-thaw stages. <b><i>Conclusions:</i></b> It is concluded that 20% EG exhibits cryoprotective properties in terms of regulating morphological and biochemical traits of frozen Indian red jungle fowl sperm.</p>","PeriodicalId":55358,"journal":{"name":"Biopreservation and Biobanking","volume":" ","pages":""},"PeriodicalIF":1.6,"publicationDate":"2025-01-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142980062","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metabolic Mode of Alginate-Encapsulated Human Mesenchymal Stromal Cells as a Background for Storage at Ambient Temperature.","authors":"Natalia Trufanova, Oleksandra Hubenia, Yurii Kot, Oleh Trufanov, Ihor Kovalenko, Kateryna Kot, Oleksandr Petrenko","doi":"10.1089/bio.2024.0103","DOIUrl":"https://doi.org/10.1089/bio.2024.0103","url":null,"abstract":"<p><p><b><i>Introduction:</i></b> Human mesenchymal stromal cells (MSCs) are attractive for both medical practice and biomedical research. Nonfreezing short-term storage may provide safe and simple transportation and promote the practical use of MSCs. <b><i>Objectives:</i></b> We aimed to determine the duration of efficient storage at ambient temperature (22°C) of human dermal MSCs in different three-dimensional organization and to investigate the role of cell metabolic mode in the resistance to the ambient storage damaging factors. <b><i>Methods:</i></b> MSCs in monolayer, suspension, and encapsulated in alginate microspheres (AMS) were stored in sealed containers at 22°С in culture medium. Viability (fluorescein diacetate /ethidium bromide) and metabolic activity (Alamar Blue assay) were assessed at 0, 3, 7, 10, and 14 days of the storage. Mitochondrial membrane potential (JC-1 test), cell cycle analysis, reactive oxygen species level, and resistance to hydrogen peroxide were analyzed under culture conditions. <b><i>Results:</i></b> Alginate encapsulation was shown to maintain viability (about 85%), metabolic activity, and adhesion ability during storage for 7 days. The storage of MSCs in both monolayer and suspension was less efficient. Culture of MSCs in AMS decreased basal metabolic activity, mitochondrial activity, and led to reversible cell cycle arrest compared to standard two-dimensional culture. MSCs in AMS have a lower basal level of reactive oxygen species and higher resistance to hydrogen peroxide compared with those in monolayer culture. <b><i>Conclusion:</i></b> Revealed shift into quiescent metabolic mode is essential for alginate-encapsulated MSCs resistance to storage at ambient temperature.</p>","PeriodicalId":55358,"journal":{"name":"Biopreservation and Biobanking","volume":" ","pages":""},"PeriodicalIF":1.6,"publicationDate":"2024-12-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142900641","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Seminal Plasma-Derived Exosome Preserves the Quality Parameters of the Post-Thaw Semen of Bulls with Low Freezeability.","authors":"Rahele Ranjbar Shamsi, Razi Jafari Jozani, Reza Asadpour, Maryam Rahbar, Morteza Taravat","doi":"10.1089/bio.2024.0077","DOIUrl":"https://doi.org/10.1089/bio.2024.0077","url":null,"abstract":"<p><p><b><i>Introduction:</i></b> Sperm cryopreservation is a useful storage technique in artificial insemination. Nanoparticles and nanovesicles such as exosomes are widely used in sperm cryopreservation procedures to alleviate cold-induced injury inflicted during sperm freezing. <b><i>Objective:</i></b> The objective of the present study was to examine the impact of varying concentrations of exosomes derived from seminal plasma added to a freezing extender on the quality of post-thawed bull sperm. <b><i>Methods:</i></b> Five Holstein bulls were chosen based on their samples having less than 30% progressive motility. After exosome extraction, semen samples from bulls (<i>n</i> = 5) with progressive sperm motility ≤30% were collected, diluted with different exosome concentrations (0, 25, 50, and 100 μg/mL), and aspirated into 0.5 mL straws. After the freeze-thaw process, sperm total and progressive motility, viability, morphology, plasma membrane integrity, mitochondrial activity, and apoptosis status were assessed. Furthermore, the expression levels of annexin (ANX1), dystrophy-associated Fer-1-like protein (DYSF), fibronectin 1 (FN1), and reactive oxygen species modulator 1 (ROMO1) were evaluated via real-time polymerase chain reaction (PCR). <b><i>Results:</i></b> Adding different concentrations of exosomes (25, 50, and 150 μg/mL) significantly increased the progressive motility, viability, and membrane integrity of sperm compared with the control group (<i>p</i> < 0.05). For the apoptosis index, treatment with 100 μg/mL exosomes significantly increased the percentage of live cells (<i>p</i> < 0.05), while the percentage of necrotic cells decreased significantly (<i>p</i> < 0.05) compared with 25 μg/mL exosome. The results of quantitative PCR showed that the expression levels of ANX1 were significantly (<i>p</i> < 0.05) upregulated at 50 μg/mL exosome, and the expression of ROMO1, FN1, and DYSF were downregulated upon treatment with different exosome concentrations. <b><i>Conclusions:</i></b> In conclusion, supplementing the freezing diluent with exosome-derived seminal plasma could preserve the quality parameters of the post-thaw semen of the bull with low freezeability and could be used as a helpful method for reproductive programs.</p>","PeriodicalId":55358,"journal":{"name":"Biopreservation and Biobanking","volume":" ","pages":""},"PeriodicalIF":1.6,"publicationDate":"2024-12-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142900642","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Trehalose Cryopreservation of Human Mesenchymal Stem Cells from Cord Tissue.","authors":"Nuria Izaguirre-Pérez, Gertrudis Ligero, Paula Alba Aguilar-Solana, José Antonio Carrillo-Ávila, Carmen Ruth Rodriguez-Reyes, Ida Biunno, Rocío Aguilar-Quesada, Purificación Catalina","doi":"10.1089/bio.2024.0025","DOIUrl":"https://doi.org/10.1089/bio.2024.0025","url":null,"abstract":"<p><p>Adequate hypothermic storage of human mesenchymal stem cells (hMSCs) is of fundamental importance since they have been explored in several regenerative medicine initiatives. However, the actual clinical application of hMSCs necessitates hypothermic storage for long periods, a process that requires the use of non-toxic and efficient cryo-reagents capable of maintaining high viability and differentiating properties after thawing. Current cryopreservation methods are based on cryoprotectant agents (CPAs) containing dimethylsulphoxide (DMSO), which have been shown to be toxic for clinical applications. In this study, we describe a simple and effective trehalose (TRE)-based solution to cryo-store human umbilical cord-derived MSCs (UC-MSCs) in liquid nitrogen. Cells viability, identity, chromosomal stability, proliferative and migration capacity, and stress response were assessed after cryopreservation in TRE as CPA, testing different concentrations by itself or in combination with ethylene glycol (EG). Here we show that TRE-stored UC-MSCs provided lower cell recovery rates compared with DMSO-based solution, but maintained good functional properties, stability, and differentiating potential. The best cell recovery was obtained using 0.5 M TRE with 10% EG showing no differences in the osteogenic, adipogenic, and chondrogenic differentiation capacity. A second cycle of cryopreservation in this TRE-based solution had no additional impact on the viability and morphology, although slightly affected cell migration. Furthermore, the expression of the stress-related genes, <i>HSPA1A</i>, <i>SOD2</i>, <i>TP53</i>, <i>BCL-2</i>, and <i>BAX</i>, did not show a higher response in UC-MSCs cryopreserved in 0.5 M TRE + 10% EG compared with DMSO. Together these results, in addition to ascertained therapeutic properties of TRE, provide sufficient evidence to consider TRE-based medium as a low-cost and efficient solution for the storage of human UC-MSCs cells and potentially substitute DMSO-based cryo-reagents.</p>","PeriodicalId":55358,"journal":{"name":"Biopreservation and Biobanking","volume":" ","pages":""},"PeriodicalIF":1.6,"publicationDate":"2024-12-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142900643","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Data Governance and Distribution of Biobank: A Case from a Chinese Cancer Hospital.","authors":"Jingjing Shi, Yan Guo, Na He, Wenbin Xia, Hongkun Liu, Haixin Li","doi":"10.1089/bio.2024.0081","DOIUrl":"https://doi.org/10.1089/bio.2024.0081","url":null,"abstract":"<p><p><b><i>Objectives:</i></b> To facilitate the regionalization, specialization, and digitization of biobanks, three issues regarding data collection and application must be addressed (1) integration and distribution of data governance, (2) efficiency and efficacy of data governance, and (3) sustainability of data governance. <b><i>Methods:</i></b> We collaborated with stakeholders to identify priorities and assess infrastructure needs through the continuous evaluation and analysis of projects. We developed data management solutions, catalogs, and data models to optimize and support data collection, distribution, and application. Furthermore, ontologies were used to facilitate data integration from multiple sources, and Minimum Information About BIobank Data Sharing (MIABIS) was defined as accessible to all patients. To enhance data integrity, we conducted retrospective and prospective follow-up studies. <b><i>Results:</i></b> We completed infrastructure upgrades to match technical solutions and research demands. An information management software with six primary functional divisions was developed for data governance. We optimized the database structure and changed the biospecimen accumulation model from biospecimen-based to patient-centered and service-oriented. Subsequently, we specified 85 attributes of MIABIS to describe the biobank contents. A dual-pillar approach was adopted to expand the biobank's data in collaboration with other institutions, and MIABIS served as a bridge for both vertical and horizontal networks. From 2003 to 2021, we collected a total of 156,997 patient biospecimens/data from 20 cancer types, matching 53,113 cases from follow-up surveys. In addition, we supplied more than 40,000 biospecimens/data points for above 300 scientific research projects. <b><i>Conclusions:</i></b> An appropriate information platform for a biobank is fundamental to data collection, distribution, and application, particularly in the context of data-intensive research. We implemented a standardized scientific data structure to fulfill the research requirements. The sustainable development of a biobank depends on a scientific, standardized, and service-oriented data governance approach, along with the efficient utilization of emerging technologies.</p>","PeriodicalId":55358,"journal":{"name":"Biopreservation and Biobanking","volume":" ","pages":""},"PeriodicalIF":1.6,"publicationDate":"2024-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142820128","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bridging Financial Challenges in Young Biobanks-Funding Strategies from the Central Biobank Regensburg.","authors":"Deborah Seidler, Lina Winter, Marie Karlíková, Ondrej Topolčan, Katja Steiger, Kateřina Nováková, Ralph Burkhardt, Tanja Niedermair, Christoph Brochhausen","doi":"10.1089/bio.2023.0129","DOIUrl":"https://doi.org/10.1089/bio.2023.0129","url":null,"abstract":"","PeriodicalId":55358,"journal":{"name":"Biopreservation and Biobanking","volume":" ","pages":""},"PeriodicalIF":1.6,"publicationDate":"2024-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142820127","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}