Biotechnology & Genetic Engineering Reviews最新文献_第3页

Effect of interventional embolization based on absolute ethanol for peripheral arteriovenous malformations. 基于绝对乙醇的介入栓塞治疗外周动静脉畸形的效果。

IF 6.5 3区工程技术

Biotechnology & Genetic Engineering Reviews Pub Date : 2024-12-01 Epub Date: 2023-05-29 DOI: 10.1080/02648725.2023.2217617

Yanlin Wang, Dakan Liu, Li Xiao, Song Zuo, Tengfei Ren, Changxian Dong, Tianxiao Li

{"title":"Effect of interventional embolization based on absolute ethanol for peripheral arteriovenous malformations.","authors":"Yanlin Wang, Dakan Liu, Li Xiao, Song Zuo, Tengfei Ren, Changxian Dong, Tianxiao Li","doi":"10.1080/02648725.2023.2217617","DOIUrl":"10.1080/02648725.2023.2217617","url":null,"abstract":"Background: Interventional embolization schedules based on absolute ethanol are usually used for peripheral arteriovenous malformations (PAVMs), and clinicians often choose the scheme according to the classification.Aim: To evaluate different interventional embolization schedules based on absolute ethanol for PAVMs.Methods: A retrospective study was performed of 165 patients with PAVMs treated with interventional embolization based on absolute ethanol in Henan Provincial People's Hospital from January 2018 to May 2021. PAVMs were classified as type II (n = 67), type III (n = 81) and type IV (n = 17) according to the Yakes classification system, including 123 maxillofacial, 13 trunk and 29 limbs. Effectiveness of embolization was based on PAVM devascularization on angiography: 100% (total), 90%~99% (near-total), 70%~90% (substantial), 30%~70% (partial) and 0%~30% (failure).Results: PAVMs were classified as type II (n = 67), type III (n = 81) and type IV (n = 17) according to the Yakes classification system, including 123 maxillofacial (74.55%), 13 trunk (7.88%) and 29 limbs (17.58%). There are statistical differences in the angiographic outcomes among different Yakes classification and between different methods (P < 0.05), and there was a statistical difference in the failure rates among different Yakes classification (P < 0.05).Conclusions: PAVMs occur maxillofacial usually, and Type II can achieve better effect by spring coil and absolute ethanol, while Type III and Type IV have no ideal effect by Pingyangmycin + iodized oil + PVA + absolute ethanol and spring coil + absolute ethanol, respectively. Both the two happen to be complications, and wound accounts the highest.","PeriodicalId":55355,"journal":{"name":"Biotechnology & Genetic Engineering Reviews","volume":" ","pages":"4899-4910"},"PeriodicalIF":6.5,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9533707","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effectiveness of psychological interventions on pregnancy rates in infertile women undergoing assisted reproductive technologies: a meta-analysis of randomised controlled trials. 心理干预对接受辅助生殖技术治疗的不孕妇女怀孕率的影响：随机对照试验荟萃分析。

IF 6.5 3区工程技术

Biotechnology & Genetic Engineering Reviews Pub Date : 2024-12-01 Epub Date: 2023-05-18 DOI: 10.1080/02648725.2023.2213080

Chaorong Bian, Jie Cao, Kejin Chen, Xiyang Xia, Xuexia Yu

{"title":"Effectiveness of psychological interventions on pregnancy rates in infertile women undergoing assisted reproductive technologies: a meta-analysis of randomised controlled trials.","authors":"Chaorong Bian, Jie Cao, Kejin Chen, Xiyang Xia, Xuexia Yu","doi":"10.1080/02648725.2023.2213080","DOIUrl":"10.1080/02648725.2023.2213080","url":null,"abstract":"The purpose of this study was to assess the effects of psychological interventions on the pregnancy rates of infertile women undergoing assisted reproductive technology (ART). Using the electronic databases PubMed, EM Base, Cochrane Library, WOS, CNKI, WanFang Data, CSTJ, and CBM, a systematic literature search was conducted in the second week of August 2019. Randomized controlled trials (RCTs) on the effect of psychological interventions on the pregnancy rate of infertile women undergoing assisted reproductive technology were collected. There is no time limit for this search setting. The language is limited to Chinese or English. Two investigators independently screened the literature, extracted data, and assessed the risk of bias of the included studies, and then used Revman5.3 and STATA16.0 software for meta-analysis. A total of 25 randomized controlled trials were included in this meta-analysis, including 2098 patients in the experimental group and 2075 patients in the control group. There was a significant difference in the pregnancy rate between the two groups [RR=1.31, 95%CI(1.22,1.40)]. Subgroup analysis showed that this is also true of infertile women of different nationalities, different intervention timing and format. However, different psychological interventions may indeed have different effects. Current evidence suggests that psychological interventions may improve pregnancy rates in infertile women undergoing assisted reproductive technology. Limited by the quantity and quality of included studies, the above conclusions need to be verified by more high-quality studies. Our PROSPERO registration number is: CRD42019140666.","PeriodicalId":55355,"journal":{"name":"Biotechnology & Genetic Engineering Reviews","volume":" ","pages":"4512-4531"},"PeriodicalIF":6.5,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9541801","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Role of gut microbiota in tumorigenesis and antitumoral therapies: an updated review. 肠道微生物群在肿瘤发生和抗肿瘤疗法中的作用：最新综述。

IF 6.5 3区工程技术

Biotechnology & Genetic Engineering Reviews Pub Date : 2024-12-01 Epub Date: 2023-01-12 DOI: 10.1080/02648725.2023.2166268

Shivani Singhal, Renu Bhadana, Buddhi Prakash Jain, Akash Gautam, Shweta Pandey, Vibha Rani

{"title":"Role of gut microbiota in tumorigenesis and antitumoral therapies: an updated review.","authors":"Shivani Singhal, Renu Bhadana, Buddhi Prakash Jain, Akash Gautam, Shweta Pandey, Vibha Rani","doi":"10.1080/02648725.2023.2166268","DOIUrl":"10.1080/02648725.2023.2166268","url":null,"abstract":"Gut microbiota plays a prominent role in regulation of host nutrientmetabolism, drug and xenobiotics metabolism, immunomodulation and defense against pathogens. It synthesizes numerous metabolites thatmaintain the homeostasis of host. Any disbalance in the normalmicrobiota of gut can lead to pathological conditions includinginflammation and tumorigenesis. In the past few decades, theimportance of gut microbiota and its implication in various diseases, including cancer has been a prime focus in the field of research. Itplays a dual role in tumorigenesis, where it can accelerate as wellas inhibit the process. Various evidences validate the effects of gutmicrobiota in development and progression of malignancies, wheremanipulation of gut microbiota by probiotics, prebiotics, dietarymodifications and faecal microbiota transfer play a significant role.In this review, we focus on the current understanding of theinterrelationship between gut microbiota, immune system and cancer,the mechanisms by which they play dual role in promotion andinhibition of tumorigenesis. We have also discussed the role ofcertain bacteria with probiotic characteristics which can be used tomodulate the outcome of the various anti-cancer therapies under theinfluence of the alteration in the composition of gut microbiota.Future research primarily focusing on the microbiota as a communitywhich affect and modulate the treatment for cancer would benoteworthy in the field of oncology. This necessitates acomprehensive knowledge of the roles of individual as well asconsortium of microbiota in relation to physiology and response ofthe host.","PeriodicalId":55355,"journal":{"name":"Biotechnology & Genetic Engineering Reviews","volume":" ","pages":"3716-3742"},"PeriodicalIF":6.5,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10525375","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Expression of ALDH1 plays the important role during generation and progression in human cervical cancer. ALDH1 的表达在人类宫颈癌的发生和发展过程中发挥着重要作用。

IF 6.5 3区工程技术

Biotechnology & Genetic Engineering Reviews Pub Date : 2024-12-01 Epub Date: 2022-12-25 DOI: 10.1080/02648725.2022.2161211

Li Zhang, Bingying Dong, Xiaoli Yuan

{"title":"Expression of ALDH1 plays the important role during generation and progression in human cervical cancer.","authors":"Li Zhang, Bingying Dong, Xiaoli Yuan","doi":"10.1080/02648725.2022.2161211","DOIUrl":"10.1080/02648725.2022.2161211","url":null,"abstract":"Cervical cancer which is caused by persistent infection with oncogenic human papillomavirus (HPV), is the third most common cancer. HPV infection causes the progression of the normal cervix to cervical intraepithelial neoplasia (CIN) because it often occurs at the function conversion of the cervical squamous epithelium and columnar epithelium zone, further to invasive carcinoma. The difference in the ALDH1 expression was very significant. With the progression of cervical cancer, reports explained obviously increased nuclear and cytoplasm ALDH1 staining in comparisons of cervical carcinomas and normal cervix (P < 0.0001), cervical carcinomas compared with CIN (P = 0.0002). Therefore, ALDH1 as a stem marker, not only resists cervical cancer but also resists in normal cervix and CIN tissues. Developing an experimental method to discover cervical cancer earlier is feasible. Furthermore, the ALDH1 was expressed in human cervical cancer cell lines (Hela, SiHa, CaSki, HT-3, and C33A) together with western blot and immunocytochemical analysis. ALDH1 plays a significant role in nuclear and cytoplasm staining by immunochemistry in single or clustered HT-3 and C33A cells. However, western blot and immunochemical analysis did not detect ALDH1 in HeLa or CaSki, SiHa cells. We also discovered that there were no remarkable differences in age, tumor size, clinical TNM staging, multiple pelvic lymph node metastasis, or histological staging (p > 0.05) between the ALDH1-positive groups in 100 cervical cancer tissues. But after the control variable age, different ALDH rating survival function contrasted, it can be concluded that the higher ALDH1 scores with the survival of patients with the worse condition.","PeriodicalId":55355,"journal":{"name":"Biotechnology & Genetic Engineering Reviews","volume":" ","pages":"3768-3779"},"PeriodicalIF":6.5,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9720129","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Integrated bioinformatics analysis for exploring hub genes and related mechanisms affecting the progression of gastric cancer. 综合生物信息学分析，探索影响胃癌进展的枢纽基因和相关机制。

IF 6.5 3区工程技术

Biotechnology & Genetic Engineering Reviews Pub Date : 2024-12-01 Epub Date: 2023-05-27 DOI: 10.1080/02648725.2023.2218201

Yu Wang, Di Li, Dan Li, Honglei Wang, Yu Wu

{"title":"Integrated bioinformatics analysis for exploring hub genes and related mechanisms affecting the progression of gastric cancer.","authors":"Yu Wang, Di Li, Dan Li, Honglei Wang, Yu Wu","doi":"10.1080/02648725.2023.2218201","DOIUrl":"10.1080/02648725.2023.2218201","url":null,"abstract":"Objective Gastric cancer (GC) is a high-risk tumor disease worldwide. The goal of the current study was to explore new diagnostic and prognostic indicators for gastric cancer. Methods Database GSE19826 and GSE103236 were gained from the Gene Expression Omnibus (GEO) to screen for differentially expressed genes (DEGs), which were then grouped together as co-DEGs. GO and KEGG pathway analysis were used to investigate the function of these genes. The protein-protein interaction (PPI) network of DEGs was constructed by STRING. Results GSE19826 selected 493 DEGs in GC and gastric normal tissues, including 139 up-regulated genes and 354 down-regulated genes. A total of 478 DEGs were selected by GSE103236, including 276 up-regulated genes and 202 downregulated genes. 32 co-DEGs were overlapped from two databasesand involved in digestion, regulation of response to wounding, wound healing, potassium ion imports across plasma membrane, regulation of wound healing, anatomical structure homeostasis, and tissue homeostasis. KEGG analysis revealed that co-DEGs were mainly involved in ECM-receptor interaction, tight junction, protein digestion and absorption, gastric acid secretion and cell adhesion molecules. Twelve hub genes were screened by Cytoscape, including cholecystokinin B receptor (CCKBR), Collagen type I alpha 1 (COL1A1), COL1A2, COL2A1, COL6A3, COL11A1, matrix metallopeptidase 1 (MMP1), MMP3, MMP7, MMP10, tissue inhibitor of matrix metalloprotease 1 (TIMP1) and secreted phosphoprotein 1 (SPP1). Conclusions Twelve key genes affecting the progression of gastric cancer were obtained by bioinformatics, which may be potential biomarkers for the diagnosis and prognosis of GC.","PeriodicalId":55355,"journal":{"name":"Biotechnology & Genetic Engineering Reviews","volume":" ","pages":"4911-4922"},"PeriodicalIF":6.5,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9898487","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A review on influence of biochar amendment on soil processes and environmental remediation. 生物炭改良对土壤过程和环境修复的影响综述。

IF 6.5 3区工程技术

Biotechnology & Genetic Engineering Reviews Pub Date : 2024-12-01 Epub Date: 2023-02-06 DOI: 10.1080/02648725.2022.2122288

Sadia Aziz, Shabana Bibi, Mohammad Mehedi Hasan, Partha Biswas, Muhammad Ishtiaq Ali, Muhammad Bilal, Hitesh Chopra, Nobendu Mukerjee, Swastika Maitra

{"title":"A review on influence of biochar amendment on soil processes and environmental remediation.","authors":"Sadia Aziz, Shabana Bibi, Mohammad Mehedi Hasan, Partha Biswas, Muhammad Ishtiaq Ali, Muhammad Bilal, Hitesh Chopra, Nobendu Mukerjee, Swastika Maitra","doi":"10.1080/02648725.2022.2122288","DOIUrl":"10.1080/02648725.2022.2122288","url":null,"abstract":"Biochar is the thermal degradation product of biomass generated in an oxygen-limited environment under different pyrolysis conditions. Biochar characteristics are functions of the feedstock material and pyrolysis temperature. Depending on pyrolysis conditions biochar concentrates varying quantities of recalcitrant and labile carbon along with nutrients which in turn affect soil physiochemical properties and microbial processes. Biochar in soil balances carbon content encourages nitrogen fixation and solubilize phosphorus along with enhancing soil enzyme activity. It serves as a microhabitat for microorganisms present in soil thus influences the diversity, composition, and distribution of soil microbial communities by affecting their intra- and interspecific communication. This review provides an overview of the current knowledge about biochar characteristics, its interactions with soil, and associated biota and its role in soil remediation. In addition, this paper also discussed the factors affecting the capacity of biochar to adsorb organic pollutants following different mechanisms. Being an effective adsorbent due its high specific surface area, large porosity, and numerous surface functional groups biochar has been explored extensively in field of environment to remediate contaminated soils.","PeriodicalId":55355,"journal":{"name":"Biotechnology & Genetic Engineering Reviews","volume":" ","pages":"3270-3304"},"PeriodicalIF":6.5,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10668137","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Clinical effect of laparoscopic cholecystectomy in the treatment of chronic cholecystitis with gallstones. 腹腔镜胆囊切除术治疗慢性胆囊炎伴胆结石的临床效果。

IF 6.5 3区工程技术

Biotechnology & Genetic Engineering Reviews Pub Date : 2024-12-01 Epub Date: 2023-04-26 DOI: 10.1080/02648725.2023.2204710

Shigang Yu, Shucheng Shi, Xuefeng Zhu

{"title":"Clinical effect of laparoscopic cholecystectomy in the treatment of chronic cholecystitis with gallstones.","authors":"Shigang Yu, Shucheng Shi, Xuefeng Zhu","doi":"10.1080/02648725.2023.2204710","DOIUrl":"10.1080/02648725.2023.2204710","url":null,"abstract":"Chronic cholecystitis is a common disease that causes inflammation in the gallbladder and is usually associated with gallstones. Laparoscopic cholecystectomy has been widely used as a minimally invasive surgical technique to treat this condition. However, the clinical effect of laparoscopic cholecystectomy in the treatment of chronic cholecystitis with gallstones needs further investigation. This study aimed to investigate the clinical effect of laparoscopic cholecystectomy in treating chronic cholecystitis with gallstones. 90 patients with chronic cholecystitis and gallstones were randomly divided into control and research groups. The control group underwent traditional open cholecystectomy, while the research group received laparoscopic cholecystectomy. Perioperative indexes, oxidative stress indexes, serum inflammatory factors, liver function indexes and the incidence of complications were observed and compared. Results showed that laparoscopic cholecystectomy significantly reduced the operation time, blood loss, anal exhaust time, abdominal pain duration, and hospital stay compared to traditional open cholecystectomy (P < 0.05). Moreover, laparoscopic cholecystectomy significantly reduced the levels of oxidative stress indexes (GSH-Px), inflammatory factors (IL-6, TNF-α, and CRP), and liver function indexes (TBIL, AST, and ALT) compared to traditional open cholecystectomy. Moreover, the complication rate of the research group was significantly lower than that of the control group (P < 0.05). In conclusion, laparoscopic cholecystectomy for chronic cholecystitis with gallstones is a safe and effective procedure that reduces the perioperative stress response and promotes the rapid recovery of the postoperative body. The findings of this study provide a basis for the clinical promotion of laparoscopic cholecystectomy as the preferred surgical treatment for chronic cholecystitis with gallstones.","PeriodicalId":55355,"journal":{"name":"Biotechnology & Genetic Engineering Reviews","volume":" ","pages":"4000-4012"},"PeriodicalIF":6.5,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9353515","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Protective effect of dihydromyricetin against lipopolysaccharide-induced HK2 cells by upregulating HIF-1α. 二氢杨梅素通过上调 HIF-1α 对脂多糖诱导的 HK2 细胞有保护作用

IF 6.5 3区工程技术

Biotechnology & Genetic Engineering Reviews Pub Date : 2024-12-01 Epub Date: 2023-05-25 DOI: 10.1080/02648725.2023.2216549

Jiang Chen, Chao Xie, Zhen Yu

{"title":"Protective effect of dihydromyricetin against lipopolysaccharide-induced HK2 cells by upregulating HIF-1α.","authors":"Jiang Chen, Chao Xie, Zhen Yu","doi":"10.1080/02648725.2023.2216549","DOIUrl":"10.1080/02648725.2023.2216549","url":null,"abstract":"Sepsis complicated by acute kidney injury (AKI) carries an extremely high mortality rate. The present study aimed to investigate the protective effect and underlying mechanism of dihydromyricetin (DHM) on human renal tubular epithelial cells (HK2) during AKI. To simulate an in vitro model of AKI, HK2 cells were treated with lipopolysaccharide (LPS) and divided into four groups: Control, LPS, LPS+DHM, and LPS+DHM+si-HIF-1α. The cellular viability of HK2 cells was determined by the CCK-8 assay after treatment with LPS and DHM (60 μmol/L). The expression of Bcl-2, Bax, Cleaved Caspase-3, and HIF-1α was measured by Western blotting. The expression of Bcl-2, Bax, and HIF-1α mRNA was assessed by PCR. The apoptosis rate of each group was determined by flow cytometry, while different kits were used to measure the levels of MDA, SOD, and LDH in each group of HK2 cells. DHM was found to increase the expression of HIF-1α in HK2 cells after treatment with LPS. the expression of HIF-1α mRNA and protein, Cleaved Caspase-3, Bax protein, MDA and LDH levels, and apoptosis rate were significantly decreased, while Bcl-2 protein, cell viability, and SOD activity were markedly increased in the LPS+DHM group compared with the LPS and LPS+DHM+si-HIF-1α groups. Thus, DHM can reduce apoptosis and oxidative stress damage in HK2 cells by increasing HIF-1α expression after LPS treatment. DHM may be a treatment for AKI, but in vitro studies must be validated in animal models and clinical trials before drawing conclusions. Caution must be exercised in interpreting in vitro results.","PeriodicalId":55355,"journal":{"name":"Biotechnology & Genetic Engineering Reviews","volume":" ","pages":"4798-4808"},"PeriodicalIF":6.5,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9517727","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

CDCA7 serves as a novel prognostic marker in human hepatocellular carcinoma. CDCA7 是人类肝细胞癌的新型预后标志物。

IF 6.5 3区工程技术

Biotechnology & Genetic Engineering Reviews Pub Date : 2024-12-01 Epub Date: 2023-05-29 DOI: 10.1080/02648725.2023.2216072

Yuan Tian, Wenwen Han, Long Fu, Kaiji Lv, Shugeng Wu

{"title":"CDCA7 serves as a novel prognostic marker in human hepatocellular carcinoma.","authors":"Yuan Tian, Wenwen Han, Long Fu, Kaiji Lv, Shugeng Wu","doi":"10.1080/02648725.2023.2216072","DOIUrl":"10.1080/02648725.2023.2216072","url":null,"abstract":"c-Myc oncogene plays an important role in tumorigenesis, cell division cycle associated 7 (CDCA7), recently found that it is a direct target gene of c-Myc, is upregulated in many tumors, but its role in tumor progression is still poorly understood. CDCA7 expression and prognosis were analyzed in hepatocellular carcinoma using TIMER2.0 and Kaplan-Meier databases, while genomic changes were studied using cbioportal. LinkedOmics identified relevant genes and WebGestalt analyzed the associated pathways. Protein interaction networks were explored using the STRING database, and the core PPI network was analyzed with the MCODE plugin of Cytoscape. CDCA7 expression was detected in 30 paired HCC specimens by real-time PCR, and its effect on HCC cell proliferation was determined in vitro. CDCA7 expression was frequently up-regulated in human hepatocellular carcinoma (HCC), and its expression was positively correlated with prognosis. The TIMER2.0 database showed that CDCA7 was differentially expressed in hepatocellular carcinoma, with high expression in tumor tissues and low expression in normal tissues. The Kaplan-Meier database shows that high CDCA7 expression has a worse prognosis. The cBioportal database showed that the genomic change rate of CDCA7 in hepatocellular carcinoma was 2.15%, including mutations, amplifications, and deep deletions. Pathway analysis of related genes showed that CDCA7-related genes were mainly focused on cell division-related pathways. The experimental results also validate our study. CDCA7 could contribute to HCC progression and raise the possibility that CDCA7 is a potential new therapeutic target for HCC treatment.","PeriodicalId":55355,"journal":{"name":"Biotechnology & Genetic Engineering Reviews","volume":" ","pages":"4696-4712"},"PeriodicalIF":6.5,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9533710","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Study on the effect and mechanisms of piperine against cervical cancer based on network pharmacology and experimental validation. 基于网络药理学和实验验证的胡椒碱抗宫颈癌作用及机制研究

IF 6.5 3区工程技术

Biotechnology & Genetic Engineering Reviews Pub Date : 2024-12-01 Epub Date: 2023-05-26 DOI: 10.1080/02648725.2023.2217611

Hui Cheng, Yanyu Li, Jie Cheng, Yanling Zhang, Bei Zhang

{"title":"Study on the effect and mechanisms of piperine against cervical cancer based on network pharmacology and experimental validation.","authors":"Hui Cheng, Yanyu Li, Jie Cheng, Yanling Zhang, Bei Zhang","doi":"10.1080/02648725.2023.2217611","DOIUrl":"10.1080/02648725.2023.2217611","url":null,"abstract":"Piperine has immunomodulatory and anti-inflammatory properties, and its potential in treating cervical cancer needs further exploration. Using data from The Cancer Genome Atlas (TCGA), we identified immune-related differentially expressed genes (IRDEGs) in cervical cancer. Predicted targets of piperine were compared with cervical cancer-associated genes from various databases. Protein-protein interaction (PPI) network analysis, enrichment of GO and KEGG pathways, and molecular docking were performed. Kaplan-Meier survival analysis was done to assess prognostic significance. In vitro and in vivo experiments were conducted to confirm findings. We obtained 403 IRDEGs, 125 piperine targets, and 7037 cervical cancer genes. PPI network analysis revealed potential targets and pathways regulated by piperine. Molecular docking showed good binding activity of piperine with specific targets. In vitro, piperine inhibited cervical cancer cell proliferation, migration, and invasion, and promoted apoptosis. In vivo, piperine suppressed tumor growth and downregulated expression of IL-1β and NLRP3 in tumor cells. Piperine also downregulated expression of IL-17A, IL-21, IL-22, and RORγt, and decreased the number of Th17 cells in tumor tissues. Piperine may inhibit cervical cancer progression through modulation of Th17 cell activation mediated by the NLRP3/IL-1β axis. Further studies are warranted to explore the potential of piperine as an immunomodulatory agent in cervical cancer treatment.","PeriodicalId":55355,"journal":{"name":"Biotechnology & Genetic Engineering Reviews","volume":" ","pages":"4875-4898"},"PeriodicalIF":6.5,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9577623","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0