Biotechnology & Genetic Engineering Reviews最新文献

In vitro/ in vivo evaluation of double crosslinked bone glue with different degrees. 对不同程度的双交联骨胶进行体外/体内评估。

IF 6.5 3区工程技术

Biotechnology & Genetic Engineering Reviews Pub Date : 2024-12-01 Epub Date: 2023-04-20 DOI: 10.1080/02648725.2023.2203008

Wei Fan, Yijie Liu, Fushan Hou, Feng Zhao, Binqiang Wu, Weimin Jiang

{"title":"In vitro/ in vivo evaluation of double crosslinked bone glue with different degrees.","authors":"Wei Fan, Yijie Liu, Fushan Hou, Feng Zhao, Binqiang Wu, Weimin Jiang","doi":"10.1080/02648725.2023.2203008","DOIUrl":"10.1080/02648725.2023.2203008","url":null,"abstract":"Successful bone fragment fixation is a crucial factor in bone fracture healing, the fixation of crushed bone fragments could hinder bone fracture healing. Thus, ideal bone glues to effectively adhere and splice comminuted bone fragments are needed in clinical. Herein, an osteoinductive and biodegradable double cross-linked bone glue (GelMA-oDex-AMBGN) was constructed through Schiff's base reaction between commercial GelMA (with different substitution degrees of amino groups) and Odex mixed with amine-modified mesoporous bioactive glass nanoparticles (AMBGN), followed by crosslink with blue light irradiation. The GelMA-oDex-AMBGN bone glue successfully adhered and spliced the comminuted bone fragments of isolated rat skulls. GelMA-oDex-AMBGN promoted the proliferation of 3T3 cells and enhanced the expression of osteogenic proteins Runx2 and OCN in vitro. In rat cranial critical-sized defect models, GelMA-oDex-AMBGNs with different substitution degrees significantly increased the new bone contents at the fracture defect sites and promoted bone tissue regeneration in vivo. In conclusion, the double cross-linked bone glue (GelMA-oDex-AMBGN) was successfully constructed and can induce bone regeneration. Additionally, there was no significant difference in osteogenic activity among GelMA-oDex-AMBGNs with different substitution degrees and the equal content of AMBGN.","PeriodicalId":55355,"journal":{"name":"Biotechnology & Genetic Engineering Reviews","volume":" ","pages":"3047-3063"},"PeriodicalIF":6.5,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9384474","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Hsa_circ_0057104, by competitive adsorption of miR-627-5p, mediates CCND2 expression to promote malignant proliferation and Warburg effect of colorectal cancer. Hsa_circ_0057104 通过竞争性吸附 miR-627-5p，介导 CCND2 的表达，促进结直肠癌的恶性增殖和沃伯格效应。

IF 6.5 3区工程技术

Biotechnology & Genetic Engineering Reviews Pub Date : 2024-12-01 Epub Date: 2023-05-02 DOI: 10.1080/02648725.2023.2199243

Lin Zhang, Jian Xu, Dequan Jiang, Jing Zhang, Hongyuan Li, Zhengzhong Zhao, Zhechuan Mei

{"title":"Hsa_circ_0057104, by competitive adsorption of miR-627-5p, mediates CCND2 expression to promote malignant proliferation and Warburg effect of colorectal cancer.","authors":"Lin Zhang, Jian Xu, Dequan Jiang, Jing Zhang, Hongyuan Li, Zhengzhong Zhao, Zhechuan Mei","doi":"10.1080/02648725.2023.2199243","DOIUrl":"10.1080/02648725.2023.2199243","url":null,"abstract":"Objective: hsa_circ_0057104 (circPDK1) has been elucidated to regulate malignant behavior in pancreatic and renal cell carcinoma. The study functionally aimed at how circPDK1 modifies colorectal cancer (CRC) progression, along with its potential molecular mechanism.Methods: circPDK1 expression patterns in CRC tissues and cell lines were analyzed by RT-qPCR. circPDK1/miR-627-5p/CCND2 expression levels were changed by transient transfection. CCK-8 assay, flow cytometry, Transwell, immunoblotting, and commercial kits were utilized to measure CRC cell proliferation, apoptosis, invasion/migration, and glycolysis processes. Dual luciferase reporting assay and RIP assay were employed to evaluate the targeting relationship between circPDK1/miR-627-5p/CCND2.Results: circPDK1 was highly expressed in CRC. circPDK1 knockdown inhibited CRC cell proliferation, invasion/migration, and warburg effect and forced apoptosis. Overexpressing circPDK1 had the opposite effect. The effects of circPDK1 knockdown or circPDK1 overexpression on CRC cells were mitigated by downregulating miR-627-5p or CCND2, respectively. CircPDK1, by competitive adsorption of miR-627-5p, mediated CCND2 expression.Conclusion: CircPDK1 induces the malignant behavior of CRC by competitive adsorption of miR-627-5p mediating CCND2 expression, offering new insights into the future development of CRC-targeted drugs.","PeriodicalId":55355,"journal":{"name":"Biotechnology & Genetic Engineering Reviews","volume":" ","pages":"3839-3855"},"PeriodicalIF":6.5,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9399899","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Analysis of risk factors of lower extremity deep venous thrombosis in patients undergoing hepatobiliary surgery. 肝胆外科手术患者下肢深静脉血栓形成的风险因素分析。

IF 6.5 3区工程技术

Biotechnology & Genetic Engineering Reviews Pub Date : 2024-12-01 Epub Date: 2023-04-27 DOI: 10.1080/02648725.2023.2205199

Dan Song, Wenyuan Song, Peng Li, Hongyan Zhao, Xiaoting Lv

{"title":"Analysis of risk factors of lower extremity deep venous thrombosis in patients undergoing hepatobiliary surgery.","authors":"Dan Song, Wenyuan Song, Peng Li, Hongyan Zhao, Xiaoting Lv","doi":"10.1080/02648725.2023.2205199","DOIUrl":"10.1080/02648725.2023.2205199","url":null,"abstract":"This study analyzed records of 200 patients who underwent hepatobiliary surgery to identify factors that contribute to lower extremity venous thromboembolism (VTE). 19 patients (9.50%) developed lower extremity deep vein thrombosis. Univariate analysis revealed significant differences between the study group and the control group in terms of age, body mass index, previous thromboembolic history, hypertension, type 2 diabetes, hyperlipidemia, smoking history, times of lower extremity venipuncture, operation time, postoperative bedrest time, postoperative platelet count, postoperative D-dimer level, and postoperative C-reactive protein level (P<0.05). Multivariable logistic regression analysis identified age ≥60 years, body mass index ≥24 kg/m2, previous history of thromboembolism, hypertension, type 2 diabetes mellitus, hyperlipidemia, smoking history, number of lower extremity venipunctures ≥5, operation time ≥2 hours, postoperative bedrest time ≥48 hours, postoperative blood platelet count ≥300×109/L, postoperative D-dimer level ≥200 g/L, and postoperative C-reactive protein ≥8.0 mg/L as significant predisposing factors for lower extremity VTE. The study concludes that patients undergoing hepatobiliary surgery are at an increased risk of developing lower extremity VTE, and prevention strategies must be tailored to each patient's unique set of risk factors. This includes careful management of postoperative bed rest, monitoring of platelet count, D-dimer and C-reactive protein levels, controlling hypertension, type 2 diabetes mellitus, hyperlipidemia, and cessation of smoking. This study highlights the importance of early identification of patients at high risk of lower extremity VTE following hepatobiliary surgery and comprehensive prevention measures.","PeriodicalId":55355,"journal":{"name":"Biotechnology & Genetic Engineering Reviews","volume":" ","pages":"4108-4119"},"PeriodicalIF":6.5,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9400134","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Predictive value of serum SCCA and CYFRA21-1 levels on radiotherapy efficacy and prognosis in patients with non-small cell lung cancer. 血清SCCA和CYFRA21-1水平对非小细胞肺癌患者放疗疗效和预后的预测价值

IF 6.5 3区工程技术

Biotechnology & Genetic Engineering Reviews Pub Date : 2024-12-01 Epub Date: 2023-05-08 DOI: 10.1080/02648725.2023.2208449

Fei Chen, Xia Zhang

{"title":"Predictive value of serum SCCA and CYFRA21-1 levels on radiotherapy efficacy and prognosis in patients with non-small cell lung cancer.","authors":"Fei Chen, Xia Zhang","doi":"10.1080/02648725.2023.2208449","DOIUrl":"10.1080/02648725.2023.2208449","url":null,"abstract":"Monitoring changes in serum tumor marker concentrations can help in the early diagnosis of non-small cell lung cancer (NSCLC). However, there are few methods to monitor the efficacy and prognosis of radiotherapy in NSCLC patients. The present research aimed to explore the correlation between radiotherapy efficacy and squamous cell carcinoma antigen (SCCA) and cytokeratin 19 soluble fragment (CYFRA21-1) levels in NSCLC patients. Serum CYFRA21-1 and SCCA were detected with an automatic chemiluminescence immunoassay analyzer. Patients with NSCLC were followed up by telephone at regular intervals for 35 months. The χ2 test was used to compare clinical characteristics such as age, gender, smoking history and other count data between groups. Predictive value of serum SCCA and CYFRA21-1 on the efficacy of radiotherapy was analyzed by Receiver Operating Characteristic (ROC) curves. The survival of the patients was analyzed by Kaplan-Meier method. The serum SCCA and CYFRA21-1 levels in the NSCLC group were apparently higher by comparison with control group. The SCCA and CYFRA21-1 concentration were both positive relevant to Tumor Node Metastasis (TNM) stage. The Area Under Curve (AUC) of serum SCCA and CYFRA21-1 were 0.732 and 0.721, respectively. In addition, high serum SCCA and CYFRA21-1 levels could predict poor radiotherapy outcomes. Patients with high serum concentration of SCCA and CYFRA21-1 have shorter survival times. High serum SCCA and CYFRA21-1 levels could predict poor prognosis and unfavorable efficacy of radiotherapy in invalids with NSCLC.","PeriodicalId":55355,"journal":{"name":"Biotechnology & Genetic Engineering Reviews","volume":" ","pages":"4205-4214"},"PeriodicalIF":6.5,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9433533","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Influencing factors of prognosis in children with pulmonary atresia with intact ventricle septum after transthoracic balloon dilation of pulmonary valve and construction of a nomograph prediction model. 经胸肺动脉瓣球囊扩张术后肺动脉闭锁伴室间隔完整儿童预后的影响因素及提名图预测模型的构建。

IF 6.5 3区工程技术

Biotechnology & Genetic Engineering Reviews Pub Date : 2024-12-01 Epub Date: 2023-05-08 DOI: 10.1080/02648725.2023.2210448

Mengxuan Zou, Shuo Dong, Shun Liu, Chuhao Du, Yangxue Sun, Jie Dong, Haitao Xu, Jun Yan

{"title":"Influencing factors of prognosis in children with pulmonary atresia with intact ventricle septum after transthoracic balloon dilation of pulmonary valve and construction of a nomograph prediction model.","authors":"Mengxuan Zou, Shuo Dong, Shun Liu, Chuhao Du, Yangxue Sun, Jie Dong, Haitao Xu, Jun Yan","doi":"10.1080/02648725.2023.2210448","DOIUrl":"10.1080/02648725.2023.2210448","url":null,"abstract":"This study aimed to identify factors that affect the prognosis of children with pulmonary valve atresia and intact ventricular septum treated with transthoracic balloon dilation of the pulmonary valve. The study included 148 participants who were followed up for 5 years. Of these, 10 died, while 138 survived. Independent sample t-test and χ2 test were used to analyze clinical data of children in the death and survival groups. It was found that height, weight, body surface area, arterial oxygen saturation, degree of tricuspid regurgitation, pulmonary valve cross valve pressure difference, ICU length of stay, length of stay, reoperation intervention, and complications were statistically significant (P<0.05). ROC curve analysis of the measurement indicators with statistically significant differences showed that height, weight, body surface area, arterial oxygen saturation, ICU length of stay, and length of stay had AUCs ranging from 0.723 to 0.870. Logistic regression analysis revealed that the degree of tricuspid regurgitation, pulmonary valve cross valvular pressure difference, ICU length of stay, reoperation intervention, and complications were independent risk factors that affect the prognosis of patients with PA/IVS undergoing transthoracic balloon dilation of pulmonary valve. The study proposed a nomogram prediction model using R language software 4.0 \"rms\" package, which was validated using calibration curve and decision curve. The model had a C-index of 0.667 (95% CI: 0.643-0.786) and high degree of fit. This study provides clinicians with a prediction model to identify children with poor prognosis after treatment with transpulmonary valve balloon dilatation. .","PeriodicalId":55355,"journal":{"name":"Biotechnology & Genetic Engineering Reviews","volume":" ","pages":"4328-4340"},"PeriodicalIF":6.5,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9437022","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Differential differentiation of B cell lymphopoiesis in lethal and non-lethal murine malaria models. 致命性和非致命性小鼠疟疾模型中 B 细胞淋巴造血的差异分化。

IF 6.5 3区工程技术

Biotechnology & Genetic Engineering Reviews Pub Date : 2024-12-01 Epub Date: 2023-05-05 DOI: 10.1080/02648725.2023.2205200

Madhumita Panda, Bidyut Das, Farkad Bantun, Aditya K Panda, Mohd Wahid, Raju K Mandal, Naeem F Qusty, Shafiul Haque, Balachandran Ravindran

{"title":"Differential differentiation of B cell lymphopoiesis in lethal and non-lethal murine malaria models.","authors":"Madhumita Panda, Bidyut Das, Farkad Bantun, Aditya K Panda, Mohd Wahid, Raju K Mandal, Naeem F Qusty, Shafiul Haque, Balachandran Ravindran","doi":"10.1080/02648725.2023.2205200","DOIUrl":"10.1080/02648725.2023.2205200","url":null,"abstract":"B cells in protection against malaria and need of experiencing many episodes in humans to achieve a state of immunity is largely unknown. The cellular basis of such defects in terms of B cell generation, maturation and trafficking was studied by taking Plasmodium chabaudi, a non-lethal and Plasmodium berghei, a lethal murine model. A flow cytometry (FCF) based evaluation was used to study alterations in generation and maintenance of B cells in patients with Plasmodium falciparum malaria as well as in murine malaria models. A significant accumulation of mature B cells in bone marrow and immature B cells in circulation was a feature observed only in lethal malaria. At peak parasitaemia, both the models induce a significant decrease in T2 (transitional) B cells with expansion of T1B cells. Studies in patients with acute Pf malaria showed a significant expansion of memory B cells and TB cells with a concomitant decrease in naive2 B cells as compared with healthy controls. This study clearly demonstrates that acute malarial infection induces major disturbances in B cell development in lymphoid organs and trafficking in periphery.","PeriodicalId":55355,"journal":{"name":"Biotechnology & Genetic Engineering Reviews","volume":" ","pages":"4120-4137"},"PeriodicalIF":6.5,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9466858","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Application of wireless ultrasound for guided caudal anesthesia in children undergoing concealed penis surgery. 应用无线超声引导对接受隐匿阴茎手术的儿童进行尾部麻醉。

IF 6.5 3区工程技术

Biotechnology & Genetic Engineering Reviews Pub Date : 2024-12-01 Epub Date: 2023-05-17 DOI: 10.1080/02648725.2023.2214445

Yi Zhu, Ting Xiao, Shuibing Zhang, Zheng Chen, Zhen Du, Shuangquan Qu, Qian Yang

{"title":"Application of wireless ultrasound for guided caudal anesthesia in children undergoing concealed penis surgery.","authors":"Yi Zhu, Ting Xiao, Shuibing Zhang, Zheng Chen, Zhen Du, Shuangquan Qu, Qian Yang","doi":"10.1080/02648725.2023.2214445","DOIUrl":"10.1080/02648725.2023.2214445","url":null,"abstract":"Caudal anesthesia alleviates the strong pain endured by children during surgical treatment for concealed penis. In the traditional method, anesthesiologists identify the puncture point using the 'blind probe' method, which leads to anesthesia induction failure in children. Ultrasound has recently gained wide attention for its guidance in peripheral nerve block analgesia. However, the clinical significance of wireless ultrasound - guided caudal anesthesia technology in children remains unexplored. This study investigated the clinical value of wireless ultrasound - guided caudal anesthesia in children undergoing concealed penis surgery. From April 2022 to August 2022, 120 pediatric patients aged 3-10 years were selected for concealed penis surgery. They were divided into the wireless ultrasound - guided sacral block group (group A) and the traditional sacral block group (group B), with 60 children in each group. Children in group A and group B underwent wireless ultrasound - guided caudal anesthesia and traditional caudal anesthesia, respectively. The success rates of the first puncture and total punctures, time taken for the punctures, and number of punctures were compared between the groups. The success rates of the first puncture (95% vs 68.3%) and total puncture (100% vs 90%) were significantly higher in group A than in group B (P<0.05). The average puncture time and the average number of punctures were, respectively, significantly shorter and lesser in group A than in group B (both P<0.05). Compared with the traditional method, wireless ultrasound visualization technology can effectively improve the success rate of sacral block puncture and reduce puncture time, which is worthy of clinical application.","PeriodicalId":55355,"journal":{"name":"Biotechnology & Genetic Engineering Reviews","volume":" ","pages":"4589-4598"},"PeriodicalIF":6.5,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9469706","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Application effect of prehospital-hospital integrated emergency nursing in patients with acute cerebral infarction. 院前院内一体化急救护理在急性脑梗死患者中的应用效果。

IF 6.5 3区工程技术

Biotechnology & Genetic Engineering Reviews Pub Date : 2024-12-01 Epub Date: 2023-05-09 DOI: 10.1080/02648725.2023.2210954

Yujuan Chen, Ying Mao, Lihui Chen

{"title":"Application effect of prehospital-hospital integrated emergency nursing in patients with acute cerebral infarction.","authors":"Yujuan Chen, Ying Mao, Lihui Chen","doi":"10.1080/02648725.2023.2210954","DOIUrl":"10.1080/02648725.2023.2210954","url":null,"abstract":"This investigation aimed to elucidate the application impact of prehospital-hospital integrated emergency nursing in individuals with acute cerebral infarction (ACI). Data of 230 ACI patients admitted to our hospital from May 2021 to July 2022 were retrospectively analyzed and divided into A and B groups (AG and BG) according to different nursing methods. The inter-group comparison of the treatment time (time for physician arrival, time for completion of examination, time between admission and thrombolytic therapy, length of stay in emergency) was compared. The success rate of thrombolysis, the inter-group comparison levels of coagulation function indexes [D-dimer (D-D) and fibrinogen (Fbg)], the national Institutes of Health Stroke Scale (NIHSS) score, the Barthel score, family members' self-rating anxiety scale (SAS) score, family members' self-rating depression scale (SDS) score, family satisfaction and adverse reactions were compared between the two groups. The treatment time were reduced in the BG than in the AG (all P < 0.05). The BG had a higher success rate of thrombolysis than the AG (P < 0.05). After the therapy, the D-D level in BG group was higher than that in AG group, and Fbg was lower than that in AG group (both P < 0.05). After nursing, BG's NIHSS score was increased compared with the AG; MBI was reduced (P < 0.05); the SAS and SDS scores of the family members were also reduced (both P < 0.05). The total family satisfaction of the BG (100.00%) was greater than the AG (89.00%) (P < 0.05). The application of prehospital-hospital integrated emergency nursing in ACI patients works effectively.","PeriodicalId":55355,"journal":{"name":"Biotechnology & Genetic Engineering Reviews","volume":" ","pages":"4407-4419"},"PeriodicalIF":6.5,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9487781","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correction. 更正。

IF 6.5 3区工程技术

Biotechnology & Genetic Engineering Reviews Pub Date : 2024-12-01 Epub Date: 2023-02-02 DOI: 10.1080/02648725.2022.2126162

引用次数: 0

The influence of interventional measures on metabolic factors in fluoride and aluminum combined poisoning bones. 干预措施对氟铝联合中毒骨骼代谢因素的影响。

IF 6.5 3区工程技术

Biotechnology & Genetic Engineering Reviews Pub Date : 2024-12-01 Epub Date: 2023-05-02 DOI: 10.1080/02648725.2023.2207925

Lihua Wang, Xianghui Qin, Maojuan Yu

{"title":"The influence of interventional measures on metabolic factors in fluoride and aluminum combined poisoning bones.","authors":"Lihua Wang, Xianghui Qin, Maojuan Yu","doi":"10.1080/02648725.2023.2207925","DOIUrl":"10.1080/02648725.2023.2207925","url":null,"abstract":"This study aimed to evaluate the effects of soybean, selenium and spirulina on bone metabolic factors in rats with combined fluoride and aluminum poisoning.Methods:Sixty SD rats were allocated to five groups: A control group, a high-fluoride aluminum group, a high-fluoride aluminum plus soybean group, a high-fluoride aluminum plus selenium group, and a high-fluoride aluminum plus spirulina group. Serum samples were collected and radioimmunoassay (RIA) was used to measure osteocalcin (BGP), calcitonin (CT), parathyroid hormone (PHT) and insulin growth factor I (IGF-1) levels after 90 and 165 days of intervention. 1. When comparing the serum BGP level in the high-fluorine aluminum plus selenium group with the high-fluorine aluminum group, suggesting that the addition of selenium intervention was beneficial in reducing the effect of high-fluorine aluminum on bone homeostasis. 2. After 90 days and 165 days, the difference in serum CT levels between the intervention group and the control group was not statistically significant, suggesting an effect of the intervention factors.3. After 90 days of exposure, the three intervention groups had lower serum PTH levels (p < 0.00), inferring the efficacy of the interventions.4. After 90 days, serum IGF-1 levels were elevated in the Spirulina group compared with the high-fluorinated aluminum group, and the difference was statistically significant, suggesting that the addition of Spirulina intervention may counteract the inhibition of bone growth by high-fluorinated aluminum. The intervention of soybean, selenium, and spirulina can counterbalance the effects of fluoride and aluminum on bone metabolism and slow down the process of bone turnover.","PeriodicalId":55355,"journal":{"name":"Biotechnology & Genetic Engineering Reviews","volume":" ","pages":"4169-4180"},"PeriodicalIF":6.5,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9768965","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0