Cell Communication and Adhesion最新文献

Krüppel-like factor 4 mediates cellular migration and invasion by altering RhoA activity. kr<s:1> ppel样因子4通过改变RhoA活性介导细胞迁移和侵袭。

Cell Communication and Adhesion Pub Date : 2018-12-01 DOI: 10.1080/15419061.2018.1444034

Philip R Brauer, Jee Hun Kim, Humberto J Ochoa, Elizabeth R Stratton, Kathryn M Black, William Rosencrans, Eliza Stacey, Engda G Hagos

{"title":"Krüppel-like factor 4 mediates cellular migration and invasion by altering RhoA activity.","authors":"Philip R Brauer, Jee Hun Kim, Humberto J Ochoa, Elizabeth R Stratton, Kathryn M Black, William Rosencrans, Eliza Stacey, Engda G Hagos","doi":"10.1080/15419061.2018.1444034","DOIUrl":"https://doi.org/10.1080/15419061.2018.1444034","url":null,"abstract":"Kru¨ppel like factor 4 (KLF4) is a transcription factor that regulates genes related to differentiation and proliferation. KLF4 also plays a role in metastasis via epithelial to mesenchymal transition. Here, we investigate the function of Klf4 in migration and invasion using mouse embryonic fibroblasts and the RKO human colon cancer cell line. Compared to wild-type, cells lacking Klf4 exhibited increased migration-associated phenotypes. In addition, overexpression of Klf4 in Klf4-/- MEFs attenuated the presence of stress fibers to wild-type levels. An invasion assay suggested that lack of Klf4 resulted in increased invasive capacity. Finally, analysis of RhoA showed elevated RhoA activity in both RKO and MEF cells. Taken together, our results strongly support the novel role of KLF4 in a post-translational regulatory mechanism where KLF4 indirectly modulates the actin cytoskeleton morphology via activity of RhoA in order to inhibit cellular migration and invasion.","PeriodicalId":55269,"journal":{"name":"Cell Communication and Adhesion","volume":"24 1","pages":"1-10"},"PeriodicalIF":0.0,"publicationDate":"2018-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/15419061.2018.1444034","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35876827","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 15

Establishment and characterization of a carcinoma-associated fibroblast cell line derived from a human salivary gland adenoid cystic carcinoma. 来源于人唾液腺腺样囊性癌的癌相关成纤维细胞系的建立和表征。

Cell Communication and Adhesion Pub Date : 2018-12-01 DOI: 10.1080/15419061.2018.1464000

Jing Kong, Han Zhao, Qianhui Shang, Zhifei Ma, Ni Kang, Junling Tan, Hyat Ahmed Ibrahim Alraimi, Tingjiao Liu

{"title":"Establishment and characterization of a carcinoma-associated fibroblast cell line derived from a human salivary gland adenoid cystic carcinoma.","authors":"Jing Kong, Han Zhao, Qianhui Shang, Zhifei Ma, Ni Kang, Junling Tan, Hyat Ahmed Ibrahim Alraimi, Tingjiao Liu","doi":"10.1080/15419061.2018.1464000","DOIUrl":"https://doi.org/10.1080/15419061.2018.1464000","url":null,"abstract":"Salivary gland adenoid cystic carcinoma (SACC) is one of the most common malignancies in the oral and maxillofacial region. Carcinoma-associated fibroblast (CAF) is an important component in the tumor microenvironment and participates in SACC progression. In this study, we established a CAF cell line derived from a human SACC and named it CAF-SA. It was identified that CAF-SA expressed typical CAF biomarkers. Then, we studied the cellular communications between CAF-SA, tumor cells and endothelial cells. It was found that CAF-SA promoted the migration, invasion, and proliferation of SACC tumor cells in vitro. In addition, tube formation by endothelial cells was enhanced by CAF-SA. In vivo experiment showed that SACC cells formed larger xenografts in nude mice when they were transplanted with CAF-SA. Overall, we demonstrated that CAF-SA exhibited the most important defining feature of CAF by promoting cancer progression.","PeriodicalId":55269,"journal":{"name":"Cell Communication and Adhesion","volume":"24 1","pages":"11-18"},"PeriodicalIF":0.0,"publicationDate":"2018-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/15419061.2018.1464000","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36076239","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 9

Fabrication of nanofiber coated with l-arginine via electrospinning technique: a novel nanomatrix to counter oxidative stress under crosstalk of co-cultured fibroblasts and satellite cells. 静电纺丝技术制备l-精氨酸包覆纳米纤维:一种抗成纤维细胞和卫星细胞串扰氧化应激的新型纳米基质。

Cell Communication and Adhesion Pub Date : 2018-12-01 Epub Date: 2018-09-05 DOI: 10.1080/15419061.2018.1493107

Sivakumar Allur Subramaniyan, Sunirmal Sheet, Saravanakumar Balasubramaniam, Swami Vetha Berwin Singh, Dileep Reddy Rampa, Sureshkumar Shanmugam, Da Rae Kang, Ho Sung Choe, Kwan Seob Shim

{"title":"Fabrication of nanofiber coated with l-arginine via electrospinning technique: a novel nanomatrix to counter oxidative stress under crosstalk of co-cultured fibroblasts and satellite cells.","authors":"Sivakumar Allur Subramaniyan, Sunirmal Sheet, Saravanakumar Balasubramaniam, Swami Vetha Berwin Singh, Dileep Reddy Rampa, Sureshkumar Shanmugam, Da Rae Kang, Ho Sung Choe, Kwan Seob Shim","doi":"10.1080/15419061.2018.1493107","DOIUrl":"https://doi.org/10.1080/15419061.2018.1493107","url":null,"abstract":"The objective of this study was to synthesize and characterize novel polyurethane (PU)-nanofiber coated with l-arginine by electrospinning technique. This study determined whether l-arginine conjugated with PU-nanofiber could stimulate cell proliferation and prevent H2O2-induced cell death in satellite cells co-cultured with fibroblasts isolated from Hanwoo (Korean native cattle). Our results showed that l-arginine conjugated with PU nanofiber could reduce cytotoxicity of co-cultured satellite cells. Protein expression levels of bcl-2 were significantly upregulated whereas those of caspase-3 and caspase-7 were significantly downregulated in co-culture of satellite cells compared to those of monoculture cells after treatment with PU-nanofiber coated with l-arginine and which confirmed by Confocal microscope. These results suggest that co-culture of satellite cells with fibroblasts might be able to counter oxidative stress through translocation/penetration of antioxidant, collagen, and molecules secreted to satellite cells. Therefore, this nanofiber might be useful as a wound dressing in animals to counter oxidative stresses.","PeriodicalId":55269,"journal":{"name":"Cell Communication and Adhesion","volume":"24 1","pages":"19-32"},"PeriodicalIF":0.0,"publicationDate":"2018-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/15419061.2018.1493107","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36458333","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 8

Phosphatidylethanolamine Deficiency Impairs Escherichia coli Adhesion by Downregulating Lipopolysaccharide Synthesis, Which is Reversible by High Galactose/Lactose Cultivation 磷脂酰乙醇胺缺乏通过下调脂多糖合成影响大肠杆菌粘附，高半乳糖/乳糖培养可逆转这一过程

Cell Communication and Adhesion Pub Date : 2017-01-01 DOI: 10.1080/15419061.2017.1282468

Chuan Yu, Ming Li, Yanan Sun, Xingguo Wang, Yong Chen

引用次数: 5

Pannexin1 Single Nucleotide Polymorphism and Platelet Reactivity in a Cohort of Cardiovascular Patients Pannexin1单核苷酸多态性与心血管患者血小板反应性

Cell Communication and Adhesion Pub Date : 2017-01-01 DOI: 10.1080/15419061.2017.1282469

F. Stierlin, F. Molica, J. Reny, B. Kwak, P. Fontana

引用次数: 11

Macrophages Aggravate Hypoxia-Induced Cardiac Microvascular Endothelial Cell Injury via Peroxynitrite: Protection by Tongxinluo 巨噬细胞通过过氧亚硝酸盐加重缺氧诱导的心肌微血管内皮细胞损伤:通心络的保护作用

Cell Communication and Adhesion Pub Date : 2015-11-02 DOI: 10.3109/15419061.2016.1155565

Xiujuan Wang, Kun Liu, Bin Li, Yanning Li, Kaiwei Ye, J. Qi, Yu Wang

{"title":"Macrophages Aggravate Hypoxia-Induced Cardiac Microvascular Endothelial Cell Injury via Peroxynitrite: Protection by Tongxinluo","authors":"Xiujuan Wang, Kun Liu, Bin Li, Yanning Li, Kaiwei Ye, J. Qi, Yu Wang","doi":"10.3109/15419061.2016.1155565","DOIUrl":"https://doi.org/10.3109/15419061.2016.1155565","url":null,"abstract":"Abstract Activated macrophages contribute to endothelial dysfunction; however, it is unclear how peroxynitrite contributes to macrophage-mediated human cardiac microvascular endothelial cell (HCMEC) injury in hypoxia. In macrophage-HCMEC co-cultures subjected to hypoxia, there was an increase in hypoxia-inducible factor (HIF)-1α, HIF-2α, inducible nitric oxide synthase (iNOS), endothelin-converting enzyme (ECE)-1 and cyclooxygenase-2 (COX-2), and concomitant decrease in prostacyclin synthase (PGIS). This was mimicked by a peroxynitrite donor and attenuated by its decomposition catalyst. Tongxinluo (TXL) could decrease HIF-2α, iNOS, ECE-1 and COX-2 and increase PGIS in a dose-dependent manner, with increase of vascular endothelial growth factor. The protein alterations verified the remarkably affected mRNAs, indicating that the effects of TXL were similar to but better than that of peroxynitrite decomposition catalyst. Furthermore, TXL inhibited macrophage-mediated nitrotyrosine accumulation and attenuated HCMEC injury. The results suggest that peroxynitrite contributes to macrophage-mediated HCMEC injury in hypoxia, and TXL attenuates HCMEC injury mainly by inhibiting peroxynitrite.","PeriodicalId":55269,"journal":{"name":"Cell Communication and Adhesion","volume":"1 1","pages":"39 - 47"},"PeriodicalIF":0.0,"publicationDate":"2015-11-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89120511","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 10

Structure, Regulation and Function of Gap Junctions in Liver 肝脏间隙连接的结构、调节和功能

Cell Communication and Adhesion Pub Date : 2015-11-02 DOI: 10.3109/15419061.2016.1151875

Joost Willebrords, Sara Crespo Yanguas, M. Maes, E. Decrock, Nan Wang, L. Leybaert, T. C. da Silva, Isabel Veloso Alves Pereira, H. Jaeschke, B. Cogliati, M. Vinken

引用次数: 19

Regulation of Endothelial Cell Adherence and Elastic Modulus by Substrate Stiffness 基质刚度对内皮细胞粘附和弹性模量的调节

Cell Communication and Adhesion Pub Date : 2015-11-02 DOI: 10.1080/15419061.2016.1265949

Sharareh Jalali, M. Tafazzoli-Shadpour, N. Haghighipour, Ramin Omidvar, F. Safshekan

{"title":"Regulation of Endothelial Cell Adherence and Elastic Modulus by Substrate Stiffness","authors":"Sharareh Jalali, M. Tafazzoli-Shadpour, N. Haghighipour, Ramin Omidvar, F. Safshekan","doi":"10.1080/15419061.2016.1265949","DOIUrl":"https://doi.org/10.1080/15419061.2016.1265949","url":null,"abstract":"Abstract Although substrate stiffness has been previously reported to affect various cellular aspects, such as morphology, migration, viability, growth, and cytoskeletal structure, its influence on cell adherence has not been well examined. Here, we prepared three soft, medium, and hard polyacrylamide (PAAM) substrates and utilized AFM to study substrate elasticity and also the adhesion and mechanical properties of endothelial cells in response to changing substrate stiffness. Maximum detachment force and cell stiffness were increased with increasing substrate stiffness. Maximum detachment force values were 0.28 ± 0.14, 0.94 ± 0.27, and 1.99 ± 0.59 nN while Young’s moduli of cells were 218.85 ± 38.73, 385.58 ± 131.67, and 933.20 ± 428.92 Pa for soft, medium, and hard substrates, respectively. Human umbilical vein endothelial cells (HUVECs) showed round to more spread shapes on soft to hard substrates, with the most organized and elongated actin structure on the hard hydrogel. Our results confirm the importance of substrate stiffness in regulating cell mechanics and adhesion for a successful cell therapy.","PeriodicalId":55269,"journal":{"name":"Cell Communication and Adhesion","volume":"2011 1","pages":"79 - 89"},"PeriodicalIF":0.0,"publicationDate":"2015-11-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86324127","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 46

Cytokine Release Patterns in Mixed Lymphocyte Culture (MLC) of T-Cells with Dendritic Cells (DC) Generated from AML Blasts Contribute to Predict anti-Leukaemic T-Cell Reactions and Patients' Response to Immunotherapy. 混合淋巴细胞培养(MLC) t细胞与AML母细胞产生的树突状细胞(DC)的细胞因子释放模式有助于预测抗白血病t细胞反应和患者对免疫治疗的反应。

Cell Communication and Adhesion Pub Date : 2015-04-01 Epub Date: 2016-09-07 DOI: 10.1080/15419061.2016.1223634

Dorothea Fischbacher, Marion Merle, Anja Liepert, Christine Grabrucker, Tanja Kroell, Andreas Kremser, Julia Dreyßig, Markus Freudenreich, Friedhelm Schuster, Arndt Borkhardt, Doris Kraemer, Claus-Henning Koehne, Hans-Jochem Kolb, Christoph Schmid, Helga Maria Schmetzer

{"title":"Cytokine Release Patterns in Mixed Lymphocyte Culture (MLC) of T-Cells with Dendritic Cells (DC) Generated from AML Blasts Contribute to Predict anti-Leukaemic T-Cell Reactions and Patients' Response to Immunotherapy.","authors":"Dorothea Fischbacher, Marion Merle, Anja Liepert, Christine Grabrucker, Tanja Kroell, Andreas Kremser, Julia Dreyßig, Markus Freudenreich, Friedhelm Schuster, Arndt Borkhardt, Doris Kraemer, Claus-Henning Koehne, Hans-Jochem Kolb, Christoph Schmid, Helga Maria Schmetzer","doi":"10.1080/15419061.2016.1223634","DOIUrl":"https://doi.org/10.1080/15419061.2016.1223634","url":null,"abstract":"To enlighten interactions between autologous, allogeneic or T-cells from patients after stem cell transplantation with leukaemia-derived-dendritic-cells containing dendritic cells or blast containing mononuclear cells (n = 21, respectively), we determined cytokine-concentrations (interleukin 2, 4, 6, 10, tumor-necrosis-factor-α, interferon-γ) in supernatants of mixed-lymphocyte-culture and in serum (n = 16) of 20 patients with acute myeloid leukaemia and three patients with myelodysplastic syndromes by cytometric-bead-assay. We correlated our data with lytic capabilities of stimulated T-cells in a fluorolysis-assay and clinical data: Dendritic-cell-/mononuclear-cell-stimulation of T-cells resulted in increased cytokine-levels in culture-medium compared to serum. There were no significant differences between cytokine-patterns of cases with/without lytic T-cell-activity, response to immunotherapy (stem cell transplantation/donor-lymphocyte-infusion) or graft-versus-host-disease. However, some predictive cytokine-cut-off-values for antileukaemic T-cell-activity, patients' response to immunotherapy and graft-versus-host-disease could be defined. Cytokine-profiles alone, without functional assays, are no useful tool to predict antileukaemic T-cell-function, although they can indicate lytic T-cell-activity, patients' response to immunotherapy and graft-versus-host-disease.","PeriodicalId":55269,"journal":{"name":"Cell Communication and Adhesion","volume":"22 2-6","pages":"49-65"},"PeriodicalIF":0.0,"publicationDate":"2015-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/15419061.2016.1223634","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34424282","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7

Glycolaldehyde-derived advanced glycation end products (glycol-AGEs)-induced vascular smooth muscle cell dysfunction is regulated by the AGES-receptor (RAGE) axis in endothelium. 乙醇醛衍生的晚期糖基化终产物(glycolage)诱导的血管平滑肌细胞功能障碍受内皮中ages受体(RAGE)轴的调控。

Cell Communication and Adhesion Pub Date : 2015-04-01 Epub Date: 2016-09-07 DOI: 10.1080/15419061.2016.1225196

Mi-Hyun Nam, Won-Rak Son, Young Sik Lee, Kwang-Won Lee

{"title":"Glycolaldehyde-derived advanced glycation end products (glycol-AGEs)-induced vascular smooth muscle cell dysfunction is regulated by the AGES-receptor (RAGE) axis in endothelium.","authors":"Mi-Hyun Nam, Won-Rak Son, Young Sik Lee, Kwang-Won Lee","doi":"10.1080/15419061.2016.1225196","DOIUrl":"https://doi.org/10.1080/15419061.2016.1225196","url":null,"abstract":"Advanced glycation end-products (AGEs) are involved in the development of vascular smooth muscle cell (VSMC) dysfunction and the progression of atherosclerosis. However, AGEs may indirectly affect VSMCs via AGEs-induced signal transduction between monocytes and human umbilical endothelial cells (HUVECs), rather than having a direct influence. This study was designed to elucidate the signaling pathway underlying AGEs-RAGE axis influence on VSMC dysfunction using a co-culture system with monocytes, HUVECs and VSMCs. AGEs stimulated production of reactive oxygen species and pro-inflammatory mediators such as tumor necrosis factor-α and interleukin-1β via extracellular-signal-regulated kinases phosphorylation and nuclear factor-κB activation in HUVECs. It was observed that AGEs-induced pro-inflammatory cytokines increase VSMC proliferation, inflammation and vascular remodeling in the co-culture system. This result implies that RAGE plays a role in AGEs-induced VSMC dysfunction. We suggest that the regulation of signal transduction via the AGEs-RAGE axis in the endothelium can be a therapeutic target for preventing atherosclerosis.","PeriodicalId":55269,"journal":{"name":"Cell Communication and Adhesion","volume":"22 2-6","pages":"67-78"},"PeriodicalIF":0.0,"publicationDate":"2015-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/15419061.2016.1225196","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34726993","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 30