Regulation of Endothelial Cell Adherence and Elastic Modulus by Substrate Stiffness

Q2 Biochemistry, Genetics and Molecular Biology

Cell Communication and Adhesion Pub Date : 2015-11-02 DOI:10.1080/15419061.2016.1265949

Sharareh Jalali, M. Tafazzoli-Shadpour, N. Haghighipour, Ramin Omidvar, F. Safshekan

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引用次数: 46

Abstract

Abstract Although substrate stiffness has been previously reported to affect various cellular aspects, such as morphology, migration, viability, growth, and cytoskeletal structure, its influence on cell adherence has not been well examined. Here, we prepared three soft, medium, and hard polyacrylamide (PAAM) substrates and utilized AFM to study substrate elasticity and also the adhesion and mechanical properties of endothelial cells in response to changing substrate stiffness. Maximum detachment force and cell stiffness were increased with increasing substrate stiffness. Maximum detachment force values were 0.28 ± 0.14, 0.94 ± 0.27, and 1.99 ± 0.59 nN while Young’s moduli of cells were 218.85 ± 38.73, 385.58 ± 131.67, and 933.20 ± 428.92 Pa for soft, medium, and hard substrates, respectively. Human umbilical vein endothelial cells (HUVECs) showed round to more spread shapes on soft to hard substrates, with the most organized and elongated actin structure on the hard hydrogel. Our results confirm the importance of substrate stiffness in regulating cell mechanics and adhesion for a successful cell therapy.

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基质刚度对内皮细胞粘附和弹性模量的调节

虽然之前有报道称底物刚度会影响细胞的各个方面，如形态、迁移、活力、生长和细胞骨架结构，但其对细胞粘附的影响尚未得到很好的研究。在这里，我们制备了三种软、中、硬聚丙烯酰胺(PAAM)基质，并利用AFM研究了基质弹性以及内皮细胞对基质刚度变化的粘附和力学性能。最大剥离力和细胞刚度随基质刚度的增加而增加。软、中、硬基质的最大分离力分别为0.28±0.14、0.94±0.27和1.99±0.59 nN，细胞的杨氏模量分别为218.85±38.73、385.58±131.67和933.20±428.92 Pa。人脐静脉内皮细胞(HUVECs)在软硬基质上呈圆形或更分散的形状，其中硬水凝胶上的肌动蛋白结构最整齐、细长。我们的研究结果证实了基质硬度在调节细胞力学和粘附方面的重要性，这对于成功的细胞治疗至关重要。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Cell Communication and Adhesion 生物-生化与分子生物学

CiteScore

2.50

自引率

0.00%

发文量

审稿时长

>12 weeks

期刊介绍： Cessation Cell Communication and Adhesion is an international Open Access journal which provides a central forum for research on mechanisms underlying cellular signalling and adhesion. The journal provides a single source of information concerning all forms of cellular communication, cell junctions, adhesion molecules and families of receptors from diverse biological systems. The journal welcomes submission of original research articles, reviews, short communications and conference reports.