Critical Reviews in Immunology最新文献_第2页

Exploring the mechanism of Isoforskolin against asthma based on network pharmacology and experimental verification 基于网络药理学和实验验证的异佛司可林抗哮喘机制探索

IF 1.3 4区医学

Critical Reviews in Immunology Pub Date : 2024-04-01 DOI: 10.1615/critrevimmunol.2024050244

Yan Fang, Shibo Sun, Chuang Xiao, Min Li, Yuanyuan Zheng, Anju Zu, Zhuang Luo

{"title":"Exploring the mechanism of Isoforskolin against asthma based on network pharmacology and experimental verification","authors":"Yan Fang, Shibo Sun, Chuang Xiao, Min Li, Yuanyuan Zheng, Anju Zu, Zhuang Luo","doi":"10.1615/critrevimmunol.2024050244","DOIUrl":"https://doi.org/10.1615/critrevimmunol.2024050244","url":null,"abstract":"Objective: In this study, network pharmacology combined with biological experimental verification was utilized to screen the targets of Isoforskolin (ISOF) and investigate the potential underlying mechanism of ISOF acting on asthma.\u0000Methods: Asthma-related targets were screened from the Genecards and DisGeNET databases. SEA and Super-PRED databases were used to obtain the targets of ISOF. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis were employed to identify key targets and enriched regulatory pathways of ISOF acting on asthma. Then, a protein-protein interaction (PPI) network was constructed via STRING database and hub genes of ISOF against asthma were further screened using molecular docking. Finally, CCK-8, qPCR, and western blotting were performed to assess the targets of ISOF in treating asthma.\u0000Results: A total of 96 drug-related-disease targets from the relevant databases were screened out. KEGG pathway enrichment analysis predicted that the target genes might be involved in the PI3K-Akt pathway. The core targets of ISOF in treating asthma were identified by the PPI network and molecular docking, including MAPK1, mTOR, and NFKB1. Consistently, in vitro experiments showed that ISOF acting on asthma was involved in inflammatory response by reducing the expression of MAPK1, mTOR, and NFKB1.\u0000Conclusions: The present study reveals that MAPK1, mTOR, and NFKB1 might be key targets of Isoforskolin in asthma treatment and the anti-asthma effect might be related to the PI3K-AKT signaling pathway.","PeriodicalId":55205,"journal":{"name":"Critical Reviews in Immunology","volume":"38 1","pages":""},"PeriodicalIF":1.3,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140570011","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Anoikis and Mitophagy-Related Gene Signature for Predicting the Survival and Tumor Cell Progression in Colon Cancer 预测结肠癌存活率和肿瘤细胞进展的无丝分裂和有丝分裂相关基因特征

IF 1.3 4区医学

Critical Reviews in Immunology Pub Date : 2024-04-01 DOI: 10.1615/critrevimmunol.2024053203

Jian Shen, Minzhe Li

{"title":"Anoikis and Mitophagy-Related Gene Signature for Predicting the Survival and Tumor Cell Progression in Colon Cancer","authors":"Jian Shen, Minzhe Li","doi":"10.1615/critrevimmunol.2024053203","DOIUrl":"https://doi.org/10.1615/critrevimmunol.2024053203","url":null,"abstract":"Background: Anoikis is a specialized form of programmed cell death and is also related mitophagy process.\u0000Objective:We aimed to identify an anoikis and mitophagy-related genes (AMRGs) prognostic model and explore the role of SPHK1 in colon cancer (CC).\u0000Methods: Bioinformatic methods were used to screen the AMRGs. Based on these genes, all the samples were divided into different subtypes. Furthermore, LASSO was conducted to optimized the AMRGs. Based on the optimal genes, a prognostic risk score model was established and evaluated. Finally, the effects of downregulated SPHK1 on the CC cell proliferation, migration, invasion, and anoikis were investigated.\u0000Results: Based on the AMRGs, all the CC samples were divided into subtype 1 and subtype 2. An AMRGs signature containing three key genes (SPHK1, CDC25C, and VPS37A) that exhibiting predicting ability in CC survival is confirmed. Subtype2 and low-risk groups exhibited better survival and higher immune cell infiltration. Moreover, down-regulated SPHK1 is related to lower cell proliferation, migration, and invasion ability, as well as higher anoikis in CC cell line (P < 0.01).\u0000Conclusion: The AMRGs risk score model exhibits promising predicting ability on patients with CC. SPHK1 might inhibit CC cell growth, migration, and invasion through stimulating anoikis.","PeriodicalId":55205,"journal":{"name":"Critical Reviews in Immunology","volume":"10 1","pages":""},"PeriodicalIF":1.3,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140570105","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification of key chondrocyte apoptosis-related genes in osteoarthritis based on weighted gene co-expression network analysis and experimental verification 基于加权基因共表达网络分析和实验验证的骨关节炎软骨细胞凋亡相关关键基因的鉴定

IF 1.3 4区医学

Critical Reviews in Immunology Pub Date : 2024-04-01 DOI: 10.1615/critrevimmunol.2024051935

Wei Wang, Junyi Hong, Tianyi Cao, Fusheng Ye, Junwei Gao, Shumei Qin

{"title":"Identification of key chondrocyte apoptosis-related genes in osteoarthritis based on weighted gene co-expression network analysis and experimental verification","authors":"Wei Wang, Junyi Hong, Tianyi Cao, Fusheng Ye, Junwei Gao, Shumei Qin","doi":"10.1615/critrevimmunol.2024051935","DOIUrl":"https://doi.org/10.1615/critrevimmunol.2024051935","url":null,"abstract":"Objective: Osteoarthritis (OA) is the primary cause of disability worldwide. Chondrocyte apoptosis has important implications for OA onset and progression. This work was designed to explore the mechanisms of chondrocyte apoptosis in OA and identify key chondrocyte apoptosis-related genes (CARGs).\u0000Methods: GSE32317 and GSE55235 datasets were acquired from the Gene Expression Omnibus (GEO) database. OA-associated module genes were determined via weighted gene co-expression network analysis (WGCNA) in GSE32317. CARGs were acquired from public databases. ClusterProfiler was employed for GO and KEGG analyses. Protein-protein interaction (PPI) network establishment was realized via STRING database and Cytoscape, and the hub genes were screened by MCC, MNC, and DMNC algorithms of cytoHubba. The diagnostic values of the hub CARGs in OA in GSE55235 was verified via receiver operating characteristic (ROC) curve analysis. C28/I2 cells were stimulated with IL-1β to establish the in vitro OA model.\u0000Results: WGCNA identified 9,141 OA-related genes and 248 CARGs, resulting in 75 CARGs in OA. GO and KEGG analyses demonstrated that the 75 CARGs were primarily enriched in response to lipopolysaccharide, transcription regulator complex, DNA-binding transcription factor binding, along with NF-kappa B and TNF signaling pathways. NFKB1 and ICAM1 were identified as the hub CARGs in OA through the three algorithms, which showed favorable prognostic values for OA. Notably, both bioinformatics analysis and in vitro assays revealed upregulated NFKB1 and ICAM1 expression in OA.\u0000Conclusions: NFKB1 and ICAM1 were the hub CARGs in OA, which might serve as the diagnostic signatures and therapeutic","PeriodicalId":55205,"journal":{"name":"Critical Reviews in Immunology","volume":"28 1","pages":""},"PeriodicalIF":1.3,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140569998","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Advances in Vaccines, Checkpoint Blockade and Chimeric Antigen Receptor based Cancer Immunotherapeutics 基于疫苗、检查点阻断和嵌合抗原受体的癌症免疫疗法的进展

IF 1.3 4区医学

Critical Reviews in Immunology Pub Date : 2024-04-01 DOI: 10.1615/critrevimmunol.2024053025

Disha Agarwal, Gaurav Sharma, Alka Khadwal, Devinder Toor, Pankaj Malhotra

引用次数: 0

Electroacupuncture Alleviates Ischemic Stroke by Activating the mTOR/SREBP1 Pathway 电针通过激活 mTOR/SREBP1 通路缓解缺血性中风

IF 1.3 4区医学

Critical Reviews in Immunology Pub Date : 2024-03-01 DOI: 10.1615/critrevimmunol.2024051934

Jiawang Lang, Jianchang Luo, Luodan Wang, Wenbin Xu, Jie Jia, Zhipeng Zhao, Boxu Lang

{"title":"Electroacupuncture Alleviates Ischemic Stroke by Activating the mTOR/SREBP1 Pathway","authors":"Jiawang Lang, Jianchang Luo, Luodan Wang, Wenbin Xu, Jie Jia, Zhipeng Zhao, Boxu Lang","doi":"10.1615/critrevimmunol.2024051934","DOIUrl":"https://doi.org/10.1615/critrevimmunol.2024051934","url":null,"abstract":"Objective: Ischemic stroke (IS) is one of the leading causes of death and disability worldwide. Electroacupuncture (EA) has been shown to exert a neuroprotective effect in IS. However, the specific anti-IS mechanisms of EA remain to be further elucidated. Thus, we aimed to investigate the anti-IS role of EA and its mechanism.\u0000Methods: By constructing a rat IS (middle cerebral artery occlusion, MCAO) model and performing EA treatment, the neurological deficit score, brain water content, and cerebral infarction were evaluated. ELISA was used to measure the levels of oxidative stress (OS)-related molecules (MDA, SOD, GSH, and CAT). Ferroptosis-related proteins (GPX4, SLC7A11, TfR1, L-ferritin, and hepcidin), neurological damage-related proteins (GFAP, Iba-1, and Nestin), α7nAChR, and mTOR pathway-related proteins (mTOR, p-mTOR, and SREBP1) in rat brain penumbra were assessed using western blotting.\u0000Results: Following EA treatment, the neurological deficit score, brain water content, cerebral infarction, and GFAP, Iba-1, and Nestin expression were reduced in the brain penumbra of MCAO rats. Additionally, EA treatment decreased MDA level and increased SOD, GSH, and CAT levels in the brain penumbra of MCAO rats. Moreover, MCAO rats showed elevated GPX4 and SLC7A11 expression and reduced TfR1, L-ferritin, and hepcidin in the brain penumbra following EA treatment. After EA treatment, α7nAChR, mTOR, p-mTOR, and SREBP1 expression were upregulated in the brain penumbra of MCAO rats.\u0000Conclusions: EA treatment inhibited OS and ferroptosis to exert a neuroprotective effect in IS, which might be realized via the activation of mTOR/SREBP1 signaling.","PeriodicalId":55205,"journal":{"name":"Critical Reviews in Immunology","volume":"32 1","pages":""},"PeriodicalIF":1.3,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140035956","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Function of steroid receptor coactivators (SRCs) in T cells and cancers: Implications for cancer immunotherapy 类固醇受体辅激活剂 (SRC) 在 T 细胞和癌症中的功能：癌症免疫疗法的意义

IF 1.3 4区医学

Critical Reviews in Immunology Pub Date : 2024-03-01 DOI: 10.1615/critrevimmunol.2024051613

Wencan Zhang, Xu Cao, Hongmin Wu, Xiancai Zhong, Yun Shi, Zuoming Sun

引用次数: 0

KIAA1429 induces the m6A modification of LINC01106 to enhance the malignancy of lung adenocarcinoma cell via JAK/STAT3 pathway KIAA1429 诱导 LINC01106 的 m6A 修饰，通过 JAK/STAT3 通路增强肺腺癌细胞的恶性程度

IF 1.3 4区医学

Critical Reviews in Immunology Pub Date : 2024-02-01 DOI: 10.1615/critrevimmunol.2024052728

Di Xu, Ziming Wang, Fajiu Li

{"title":"KIAA1429 induces the m6A modification of LINC01106 to enhance the malignancy of lung adenocarcinoma cell via JAK/STAT3 pathway","authors":"Di Xu, Ziming Wang, Fajiu Li","doi":"10.1615/critrevimmunol.2024052728","DOIUrl":"https://doi.org/10.1615/critrevimmunol.2024052728","url":null,"abstract":"Background: Sustained expression of LINC01106 in tumors is crucial for malignant phenotype of tumor cells; Nevertheless, LINC01106’s mechanisms and clinical effects in lung adenocarcinoma (LUAD) is confined. This report focus on reveal the effect of vir-like m6A methyltransferase associated (KIAA1429)-mediated N6-methyladenosine (m6A) modification to steady LINC01106 expression on LUAD progression.\u0000Methods: qRT-PCR was conducted to clarify the LINC01106 and KIAA1429 levels in LUAD tissues. LINC01106’s and KIAA1429’s functional roles were analyzed utilizing Transwell, EdU as well as CCK-8 assays. Xenograft was cconducted to verify the function of silencing LINC01106 in tumor growth. The regulatory role between LINC01106 was investigated using MeRIP, qRT-PCR as well as Actinomycin D assay. Key proteins of JAK/STAT3 (JAK2, STAT3) pathway were revealed via western blotting.\u0000Results: LINC01106 and KIAA1429 were high-expressed in LUAD, and LINC01106 were interconnected with high tumor grade, stage, and poor prognosis. Data revealed LINC01106 inhibition reduced LUAD cell proliferation, invasion as well as migration, and restrained LUAD cell tumorigenicity. Additionally, phosphorylated JAK2, STAT3 levels were reduced by LINC01106 silencing. LINC01106 was mediated by KIAA1429 to enhance its m6A modification and expression in LUAD cells. Moreover, KIAA1429 promotion abolished the malignant phenotypic suppression induced by LINC01106 lowexpression in LUAD cells.\u0000Conclusion: This research revealed LINC01106 m6A modification was enhanced by KIAA1429 to play the promotive role in LUAD development. The results may conduce to the comprehending of the act of KIAA1429-m6A-LINC0110","PeriodicalId":55205,"journal":{"name":"Critical Reviews in Immunology","volume":"18 1","pages":""},"PeriodicalIF":1.3,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139977763","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Oral Cancer: Past, present and future of NK cell-based cancer immunotherapy 口腔癌基于 NK 细胞的癌症免疫疗法的过去、现在和未来

IF 1.3 4区医学

Critical Reviews in Immunology Pub Date : 2024-02-01 DOI: 10.1615/critrevimmunol.2024052389

Kawaljit Kaur, Anahid Jewett

引用次数: 0

Effect of p-estrogen receptor at serine on its function and breast growth 对雌激素受体丝氨酸对其功能和乳腺生长的影响

IF 1.3 4区医学

Critical Reviews in Immunology Pub Date : 2024-02-01 DOI: 10.1615/critrevimmunol.2024052499

Yuan Liang, Junhui Qin, Tiancheng Ma, Tong Yang, Zhenyu Ke, Ruian Wang

{"title":"Effect of p-estrogen receptor at serine on its function and breast growth","authors":"Yuan Liang, Junhui Qin, Tiancheng Ma, Tong Yang, Zhenyu Ke, Ruian Wang","doi":"10.1615/critrevimmunol.2024052499","DOIUrl":"https://doi.org/10.1615/critrevimmunol.2024052499","url":null,"abstract":"Background: Estrogen receptor (ER) signaling plays an important role in the development and functional differentiation of breast and participates in the process of breast cancer. Activated ER can affect various aspects of the cell’s behavior, including proliferation， via modulating the expression of many downstream target genes. Phosphorylation is one of the activation pathways of ER. However, the relationship between estrogen receptor phosphorylation sites and breast development and carcinogenesis is not clear.\u0000Methods: Using Crisper-Cas9 gene editing technology, we constructed ER S309A mutant mice. Using carmine staining of mammary gland of mice at different developmental stages, we examined breast development of ER S309Amice. Using HE staining of vaginal smears of mice at the same time for 5 consecutive days, we measured the vaginal epithelial keratinocytes.\u0000Results: We established ER S309A mutant mice and observed breast defects in ER S309A mice. In addition, we observed decreased reproductive ability, estrous cycle disorder in ER S309A mice. And number of vaginal epithelial keratinocytes in the estrous cycle of ER S309A mice was decreased.\u0000Conclusion: These results suggest that phosphorylation site of ER at serine 309 is important for ER function and breast development.","PeriodicalId":55205,"journal":{"name":"Critical Reviews in Immunology","volume":"6 1","pages":""},"PeriodicalIF":1.3,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139918547","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The value of systemic immune-inflammation index and T cell subsets in the severity and prognosis of sepsis 全身免疫炎症指数和 T 细胞亚群在败血症严重程度和预后中的价值

IF 1.3 4区医学

Critical Reviews in Immunology Pub Date : 2024-02-01 DOI: 10.1615/critrevimmunol.2024051413

Hao Zhou

{"title":"The value of systemic immune-inflammation index and T cell subsets in the severity and prognosis of sepsis","authors":"Hao Zhou","doi":"10.1615/critrevimmunol.2024051413","DOIUrl":"https://doi.org/10.1615/critrevimmunol.2024051413","url":null,"abstract":"Systemic immune-inflammation index (SII) and T cell subsets show involvement in mortality risk in septic patients, and we explored their predictive value in sepsis. Subjects were categorized into the Sepsis (SP)/Septic Shock (SSP)/Septic Shock (SPS) groups. T cell subsets [T-helper (Th)1, Th2, regulatory T cells (Treg), Th17]/platelets (PLT)/neutrophils (NEU)/lymphocytes (LYM)/C-reactive protein (CRP)/procalcitonin (PCT)/interleukin (IL)-4/IL-10/fibrinogen (FIB) were measured by an automatic blood biochemical analyzer/flow cytometry/Countess II FL automatic blood cell analyzer, with SII calculated. The correlations between SII/T cell subsets with Acute Physiology and Chronic Health Evaluation (APACH) II/Sequential Organ Failure Assessment (SOFA) scores and the predictive value of SII/Th1/Th2 for septic diagnosis/prognosis were analyzed using Spearman/ROC curve/Kaplan-Meier. The three groups varied in PLT/NEU/LYM/CRP/PCT/IL-4/IL-10/FIB levels and APACH II/SOFA scores. Compared with the SP group, the other two groups showed elevated APACH II/SOFA scores and SII/Th1/Th2/Th17/Treg levels. SII/Th1/Th2 levels significantly positively correlated with APACH II/SOFA scores. SII/Th1/Th2 levels had high predictive value for septic diagnosis/prognosis, with their combination exhibiting higher predictive value. Septic patients with high SII/Th1/Th2 levels exhibited lower survival rates. Altogether, SII, Th1, and Th2 had good predictive value for the diagnosis and prognosis of patients with varying severity of sepsis, with their high levels increasing mortality in septic patients.","PeriodicalId":55205,"journal":{"name":"Critical Reviews in Immunology","volume":"2 1","pages":""},"PeriodicalIF":1.3,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139663580","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0