Cytopathology最新文献

{"title":"Impact of Cold Ischemic Time on Nucleic Acid Quality in Cytology Samples for Cancer Gene Analysis","authors":"Hideyuki Abe,&nbsp;Akihiko Kawahara,&nbsp;Ryou Makino,&nbsp;Takato Kumagae,&nbsp;Jun Akiba,&nbsp;Hironori Kusano","doi":"10.1111/cyt.13491","DOIUrl":"10.1111/cyt.13491","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Objective</h3>\u0000 \u0000 <p>Despite the growing attention on the use of cytology samples for genetic analysis, the impact of the time from sample collection to fixation, referred to as ‘cold’ ischemic time, has not been sufficiently studied. Therefore, we investigated the quality of nucleic acids prior to fixation in body cavity fluid samples, focusing on the benign/malignant status, differences in collection methods and tumour cell content.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>We analysed 49 body cavity fluid samples collected using different methods: aspiration (<i>n</i> = 21), drainage (<i>n</i> = 5), and surgery (<i>n</i> = 15). The samples were collected from 26 malignant and 23 benign cases. DNA and RNA were extracted from all samples, and their yield and quality were assessed using Agilent TapeStation. Tumour cell content was calculated as the ratio of malignant to benign cells.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>DNA and RNA yields were significantly higher in malignant than in benign cases (<i>p</i> &lt; 0.05). Regarding nucleic acid quality, there was a significant difference in RNA quality between malignant and benign cases, but no significant difference in DNA quality. Among the 26 malignant cases, there were significant differences in integrity number (RIN) and RNA percentage of nucleic acid fragments with &gt; 200 nucleotides (DV200) between samples collected via aspiration and surgery (<i>p</i> &lt; 0.05). Tumour cell content (median, 36%; range, 20%–72%) showed no correlation with nucleic acid yield or quality.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>This study's findings suggest that DNA extracted from cytology samples is highly stable, while RNA is affected by cold ischemic time. Thus, prompt fixation after collection is necessary to maintain RNA quality.</p>\u0000 </section>\u0000 </div>","PeriodicalId":55187,"journal":{"name":"Cytopathology","volume":"36 4","pages":"391-398"},"PeriodicalIF":1.2,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143756066","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Needs Assessment for an Interprofessional Module for Enhancing Competencies Required for Fine Needle Aspiration Cytology (FNAC)","authors":"Crysle Saldanha,&nbsp;Sharada Rai,&nbsp;Abhishek Chaturvedi","doi":"10.1111/cyt.13487","DOIUrl":"10.1111/cyt.13487","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <p>Fine needle aspiration cytology (FNAC) is a cost-effective, minimally invasive diagnostic tool requiring collaboration among pathologists, radiologists, nurses, and technicians for optimal outcomes. However, interprofessional collaboration remains limited, leading to diagnostic delays and reduced patient satisfaction. This study identifies the need for an interprofessional FNAC training module through a comprehensive needs assessment among the stakeholders involved in FNAC service.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>A mixed-methods approach was used, comprising a retrospective laboratory audit for the quantitative arm (2007 FNAC procedures were audited for diagnostic accuracy, turnaround time, and non-diagnostic aspirates) and focus group discussions (FGDs) and in-depth interviews (IDIs) for the qualitative arm. SPSS Version 20 was used to evaluate the quantitative data, and thematic content analysis was used to analyse the qualitative data.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Quantitative analysis of 2007 FNAC procedure revealed a diagnostic accuracy rate of 87.5%, with 12.5% discordant cases and 4.58% non-diagnostic aspirates. Key issues from qualitative research included incorrect labelling of slides and improper disposal of FNAC items. However, effective collaboration, particularly between laboratory technicians and pathologists, enhanced the smear quality.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>This needs assessment highlights critical deficiencies in FNAC services, emphasising the urgency of an interprofessional training module. Addressing gaps in guided FNACs, sample handling, waste disposal, and patient scheduling, the module can enhance diagnostic accuracy, reduce errors, and improve patient care. Implementing this initiative promotes collaboration, fostering a culture of excellence and continuous improvement in healthcare.</p>\u0000 </section>\u0000 </div>","PeriodicalId":55187,"journal":{"name":"Cytopathology","volume":"36 4","pages":"380-385"},"PeriodicalIF":1.2,"publicationDate":"2025-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143756067","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"FNA Diagnosis of Adult Rhabdomyoma of the Tongue and Floor of the Mouth","authors":"Roobashri Murugan,&nbsp;Rashmi P. Joshi,&nbsp;Pooja Vijayakumar,&nbsp;Debasis Gochhait,&nbsp;Arun Alexender","doi":"10.1111/cyt.13486","DOIUrl":"10.1111/cyt.13486","url":null,"abstract":"<div>\u0000 \u0000 <p>Adult rhabdomyoma is a rare benign tumour of skeletal muscle origin and is rarely reported on fine needle aspiration cytology. This tumour has a preference for males older than 40 years of age. The most common sites include the parapharyngeal space, larynx, salivary glands, mouth, and soft tissue of the head and neck region. Fine needle aspiration cytology (FNAC), a less invasive alternative diagnostic method than incisional biopsy, can accurately diagnose this benign neoplasm and avoid aggressive treatment. To the best of our knowledge, only 11 cases of adult rhabdomyoma are diagnosed on FNAC. We herein report one such rare case of adult rhabdomyoma of the floor of the mouth in a 39-year-old male diagnosed with FNAC, emphasising its utility in making a definite diagnosis of adult rhabdomyoma.</p>\u0000 </div>","PeriodicalId":55187,"journal":{"name":"Cytopathology","volume":"36 5","pages":"529-534"},"PeriodicalIF":1.1,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143744488","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimising Axillary Staging in Resource-Constrained Settings: A Prospective Validation of Axillary Ultrasound and Touch Imprint Cytology in Predicting Pathologically Negative Axillae in cT2-3 Breast Cancer","authors":"Balmik Chaturvedi,&nbsp;Muktesh Khandare,&nbsp;Devashish Mishra,&nbsp;Sanjay Kumar Yadav,&nbsp;Pawan Agarwal,&nbsp;Dhananjaya Sharma","doi":"10.1111/cyt.13484","DOIUrl":"10.1111/cyt.13484","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>Management of axillary lymph nodes (ALNs) in breast cancer patients remains pivotal for staging and planning therapeutic strategies. However, In low-resource settings, achieving accurate axillary staging while avoiding overtreatment remains a challenge as the majority of patients present with advanced stage. In this prospective validation study, we assessed the efficacy of axillary ultrasound (AUS) combined with touch imprint cytology (TIC) for predicting negative axillary status in cT2-3 breast cancer patients.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>This study was a prospective, single-centre validation study conducted in the Breast and Endocrine Unit of the Department of Surgery and the Department of Pathology in a tertiary teaching hospital in central India from September 2022 to April 2024. Eligible participants included adult female patients (aged ≥ 18 years) with core needle biopsy-proven invasive breast cancer classified as cT2-3, cN0, and scheduled for primary surgical treatment. The primary outcomes were the Sensitivity, specificity, positive predictive value (PPV), negative predictive value (NPV) and accuracy of the AUS + TIC approach in predicting pathologically negative axillary status.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>AUS + TIC had a sensitivity of 100% (95% CI: 47.82%–100%), a specificity of 100% (95% CI: 91.19%–100%) and an overall accuracy of 100% (95% CI: 92.13%–100%). There were no false negatives.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>Our findings suggest that the combination of AUS + TIC provides a reliable technique with high diagnostic accuracy, sensitivity, and specificity for assessing ALN in low resource settings.</p>\u0000 </section>\u0000 </div>","PeriodicalId":55187,"journal":{"name":"Cytopathology","volume":"36 4","pages":"386-390"},"PeriodicalIF":1.2,"publicationDate":"2025-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143733321","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"WHO Reporting System for Lung Cytopathology: Insights Into the Insufficient/Inadequate/Non-Diagnostic, Atypical and Suspicious for Malignancy Categories and How to Use Them","authors":"Zahra Maleki,&nbsp;Sule Canberk,&nbsp;Andrew Field","doi":"10.1111/cyt.13479","DOIUrl":"10.1111/cyt.13479","url":null,"abstract":"<p>The World Health Organization Reporting System for Lung Cytopathology (WHO System) is an international effort aiming to serve patients worldwide in all medical resource settings and improve patient care globally. It is an evidence-based standardised reporting system applicable to all respiratory cytopathology specimens. The WHO System consists of five diagnostic categories including Insufficient/Inadequate/Non-diagnostic, Benign, Atypical, Suspicious for malignancy and Malignant. Each category has an associated risk of malignancy established from the current literature and recommendations for further management to establish as precise a diagnosis as possible. The key diagnostic cytopathological criteria for each entity are established, a differential diagnosis based on cytopathological features that is globally applicable is discussed, and best practices in appropriate ancillary studies are presented. The Insufficient/Inadequate/Non-diagnostic, Atypical and Suspicious for malignancy are diagnostic categories that are particularly challenging for cytopathologists and clinicians and patients due to the organ's diversity in sampling methods, cell types and the wide variety of neoplasms. Herein, we have reviewed the role of these three categories, discussed their definitions and cytopathological criteria, appropriate applications, risk of malignancy, clinical recommendations for patient's further diagnostic workup and application of ancillary studies. The aim was to increase cytopathologists and clinicians understanding of the three categories and provide a framework for the essential discussions that should follow.</p>","PeriodicalId":55187,"journal":{"name":"Cytopathology","volume":"36 5","pages":"434-442"},"PeriodicalIF":1.1,"publicationDate":"2025-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/cyt.13479","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143722518","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"ALK+ Anaplastic Large Cell Lymphoma Diagnosed on Paediatric Pericardial Effusion Cytology","authors":"Connor Hartzell,&nbsp;Huiying Wang,&nbsp;Emily F. Mason,&nbsp;Christopher J. O'Conor","doi":"10.1111/cyt.13481","DOIUrl":"10.1111/cyt.13481","url":null,"abstract":"<p>While rare, malignant paediatric pericardial effusions are crucial to recognise. The effusion will reaccumulate without proper treatment and, when it is the first presentation of cancer, misdiagnosis risks disease progression or acute decompensation. ALK-positive anaplastic large cell lymphoma (ALK+ ALCL), a rare cause of pericardial effusion, is a CD30-positive T-cell lymphoma driven by an <i>ALK</i> translocation, most commonly t(2;5)(p23;q35), with <i>NPM1</i> as the partner gene [<span>1</span>]. The constitutively activated ALK kinase stimulates the RAS, JAK/STAT, AKT and other pathways, driving tumorigenesis [<span>1</span>]. ALK+ ALCL presents most frequently in patients &lt; 1–30 years of age as Stage III–IV disease with bulky lymphadenopathy, mediastinal masses or skin lesions [<span>1</span>]. In children, ALK+ ALCL comprises 10%–30% of all lymphomas and responds well to cytotoxic chemotherapy (5-year event-free survival of 70%) [<span>1, 2</span>].</p><p>Here, we describe pericardial effusion as the primary presentation of paediatric ALK+ ALCL. This emphasises the diagnostic value of pericardiocentesis and highlights ALK+ ALCL in the differential diagnosis of malignant paediatric pericardial effusions.</p><p>An 11-month-old male with no significant past medical history was admitted from the emergency department to the intensive care unit with hypoxic respiratory failure following 2 weeks of cough and congestion unresponsive to steroids and albuterol. Oxygen saturation measured ~85%, and the physical exam demonstrated tachypnoea and subcostal retractions. Palpable lymphadenopathy was absent. Complete blood count showed 25.1 k/μL leucocytes (normal range 4.0–14.6 k/μL; 79.1% neutrophils; 14.7% lymphocytes; 4.4% monocytes; 0.5% basophils; 1.3% immature granulocytes), 545 k/μL platelets (normal range 150–400 k/μL) and 10.7 g/dL haemoglobin (normal range 10.5–13.5 g/dL). A complete metabolic panel was largely unremarkable. Laboratory tests for viral, fungal and bacterial infections were negative. Chest radiography demonstrated bilateral pleural effusions and cardiomegaly (Figure 1A). Echocardiogram showed a large circumferential pericardial effusion and mild right atrial collapse in diastole. EKG showed preventricular contractions and questionable right ventricular hypertrophy. Computed tomography scan, performed after ALK+ ALCL was diagnosed, demonstrated an indeterminate mass in the cervical paraspinal soft tissue and innumerable enlarged intrathoracic lymph nodes, some demonstrating encasement of surrounding vascular structures (Figure 1B). No cardiac mass was identified.</p><p>Due to concern for tamponade, emergent pericardiocentesis was performed using a 21-gauge needle and 102 mL serosanguinous fluid with a protein content of 4.4 g/dL was withdrawn. From this, cytospin slides were prepared. A cell block made from the centrifuged fluid was fixed in ethanol and post-fixed in 10% neutral-buffered formalin.</p><p>The cytospin and cell ","PeriodicalId":55187,"journal":{"name":"Cytopathology","volume":"36 3","pages":"285-289"},"PeriodicalIF":1.2,"publicationDate":"2025-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/cyt.13481","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143634987","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genetic Insights Into Recurrent Granular Cell Tumours in an Adolescent: The Role of Cytology and Next-Generation Sequencing in the Diagnosis of RASopathies","authors":"Pakesh Baishya,&nbsp;Bifica S. Lyngdoh,&nbsp;Thangjam Ruchika Devi,&nbsp;Abhilash Goyal,&nbsp;Sankappa P. Sinhasan","doi":"10.1111/cyt.13483","DOIUrl":"10.1111/cyt.13483","url":null,"abstract":"<p>Recurrent multiple benign granular cell tumours were observed in an adolescent with Noonan syndrome, a RASopathy linked to dysregulated RAS/MAPK signalling. This case suggests a potential association between aberrant pathway activation and tumour development, highlighting the need for evaluating associated fatal anomalies and conducting genetic studies.</p>","PeriodicalId":55187,"journal":{"name":"Cytopathology","volume":"36 3","pages":"290-294"},"PeriodicalIF":1.2,"publicationDate":"2025-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143617857","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Digital Cytology Combined With Artificial Intelligence Compared to Conventional Microscopy for Anal Cytology: A Preliminary Study","authors":"Renê Gerhard,&nbsp;Cioly Rivero Colmenarez,&nbsp;Corinne Selle,&nbsp;Gaël Paul Hammer","doi":"10.1111/cyt.13482","DOIUrl":"10.1111/cyt.13482","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Introduction</h3>\u0000 \u0000 <p>Recent studies have shown that digital cytology (DC) coupled with artificial intelligence (AI) algorithms is a valid approach to the diagnosis of cervico-vaginal lesions using liquid-based cytology (LBC). We evaluated the use of these methods for anal LBC specimens.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>A series of 124 anal LBC slides previously diagnosed by conventional microscopy (CC) were reviewed with a DC/AI system that generated a gallery of images. Diagnoses based on the selected images, according to the 2014 Bethesda System for Reporting Cervical Cytology, were compared to CC.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Overall, CC and DC/AI approaches detected a similar number of abnormal (ASC-US+) cases (63 and 62 cases, respectively). We observed an exact concordance between CC and DC in 70 (57.9%) cases, corresponding to a moderate agreement between the two approaches (κ = 0.41, <i>p</i> &lt; 0.001). A moderate agreement (κ = 0.48, <i>p</i> &lt; 0.001) was also found when positive cases were stratified into ‘low-grade’ (ASC-US, LSIL) and ‘high-grade’ lesions (ASC-H, HSIL). The DC/AI system detected more cases of higher severity (ASC-H, HSIL: 9 and 2 cases, respectively) than CC (3 cases classified as HSIL).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>The number of ASC-US+ cases detected by both systems was similar. The DC/AI system detected more cases of higher severity compared to the CC.</p>\u0000 </section>\u0000 </div>","PeriodicalId":55187,"journal":{"name":"Cytopathology","volume":"36 3","pages":"250-258"},"PeriodicalIF":1.2,"publicationDate":"2025-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143607229","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Polymorphous Adenocarcinoma: A Cytological Enigma Unveiled Through Uncommon Locations—A Case Study","authors":"Paruvathavarthini Thambiraj,&nbsp;Debasis Gochhait,&nbsp;Neelaiah Siddaraju","doi":"10.1111/cyt.13480","DOIUrl":"10.1111/cyt.13480","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Introduction</h3>\u0000 \u0000 <p>Polymorphous adenocarcinoma (PAC) is a type encountered in minor salivary glands (49%–87%) PAC may occasionally occur in major salivary glands, in particular the parotid gland approximately 3% (range: 0%–9%) of cases. PAC has become a challenging entity for clinicians to diagnose because of the overlap of its clinical and microscopic features with other salivary gland tumours. Fine-needle aspiration (FNA) and core biopsy findings in these tumours are limited.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Aim</h3>\u0000 \u0000 <p>The main objective is to study the cytomorphological aspects of this tumour and focus on uncommon locations.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>It was a retrospective single-institutional study from a time period of 4 years. A total of 11 cases (<i>n</i> = 11) were collected from archives. Papanicolaou (Pap) and May-Grunwald Giemsa (MGG) stained cytology slides were retrieved. All clinical details, types of procedure, cell block material, immunocytochemistry, follow-up biopsy/resection specimens and immunohistochemistry (IHC) status were analysed in detail.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Total 11 cases (<i>n</i> = 11) were collected; seven cases were located in major and four were located in minor salivary glands. Histopathological correlation was seen in eight cases (<i>n</i> = 8, 73%) remaining cases were lost for follow-up (<i>n</i> = 3). All aspirates showed similar cytomorphologic features, with hypercellular smears showing branching papillae, sheets and clusters composed of bland uniform cells with dispersed myxohyaline stroma seen. Five cases had lymph node metastasis and soft tissue infiltrative tumour deposits. Out of eight cases in histopathology, five cases were resection specimens and three cases were excision biopsy.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>Cytological evaluation of PAC, especially in uncommon anatomical sites, presents inherent challenges due to its morphological variability and overlap with other salivary gland neoplasms. However, with careful sampling, comprehensive assessment and integration of ancillary techniques, cytologists can contribute significantly to the accurate diagnosis and optimal management of PAC, thereby improving patient outcomes and enhancing our understanding of this rare malignancy.</p>\u0000 </section>\u0000 </div>","PeriodicalId":55187,"journal":{"name":"Cytopathology","volume":"36 3","pages":"266-272"},"PeriodicalIF":1.2,"publicationDate":"2025-03-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143588323","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Diagnostic Value of Liquid-Based Cervical Cytology Test in Endometrial Carcinoma","authors":"Tingting He,&nbsp;Shuiping Xie,&nbsp;Chunxia Liang,&nbsp;Yan Qin","doi":"10.1111/cyt.13477","DOIUrl":"10.1111/cyt.13477","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>Abnormal endometrial cells could be detected by liquid-based cervical cytology test due to the anatomical continuity of the uterine cavity with the cervix. Our aim was to evaluate the diagnostic value of Papanicolaou (Pap) test in endometrial cancer (ECa).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>ECa cases with liquid-based Pap smears within 6 months before the histological diagnosis were retrospectively searched from an academic women's and children's hospital pathology archive. Corresponding smears were reviewed, and clinicopathological parameters and Pap test results were recorded.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>A total of 171 cases were identified. Among these patients, 43 (25.1%) cases were interpreted as abnormal, 32 (74.4%) of which suggested glandular abnormality in the routine screening (initial Pap smear reports). After re-examining all slides, 123 (72.5%) cases showed the presence of endometrial abnormality. Among these, 87 cases (70.7%) were diagnosed as atypical endometrial cells (AEC), 33 cases (26.8%) as adenocarcinoma (ADC), and 3 cases (2.4%) as benign endometrial cells. The cases with non-endometrioid histology were more likely to be interpreted cytologically as ADC, while endometrioid subtypes were often diagnosed as AEC (<i>p</i> &lt; 0.05). Except for the tumour type (<i>p</i> &lt; 0.05), the other clinicopathological factors were not significantly related to a higher percentage of positive Pap test results, including age, histological grade, myometrial invasion, cervical involvement, lymphovascular invasion, and FIGO stage(<i>p</i> &gt; 0.05).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>Pap test plays a role in the detection of ECa. Detection could be improved in routine screening by paying more attention to endometrial lesions in high-risk populations.</p>\u0000 </section>\u0000 </div>","PeriodicalId":55187,"journal":{"name":"Cytopathology","volume":"36 3","pages":"259-265"},"PeriodicalIF":1.2,"publicationDate":"2025-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143574727","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}