Cytopathology最新文献

{"title":"Thank You to Reviewers 2024","authors":"Philippe Vielh","doi":"10.1111/cyt.13470","DOIUrl":"https://doi.org/10.1111/cyt.13470","url":null,"abstract":"","PeriodicalId":55187,"journal":{"name":"Cytopathology","volume":"36 2","pages":"216-219"},"PeriodicalIF":1.2,"publicationDate":"2025-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143379872","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Bridge: Supernatant Derived From Cytological Sample Preparations.","authors":"Sinchita Roy-Chowdhuri","doi":"10.1111/cyt.13475","DOIUrl":"https://doi.org/10.1111/cyt.13475","url":null,"abstract":"<p><p>The scope and extent of molecular cytopathology in the era of precision medicine has been expanding in recent years. The versatility of cytology specimen preparations has provided ample opportunity for the cytopathology community to evolve, innovate and 'do more with less' using limited amounts of tissue. More recently, cytology-derived supernatant liquid biopsy samples have been identified as a substantial source of high-quality genomic material that can be interrogated for genotyping for therapeutic decision-making, as well as other roles in cancer screening for early-stage disease, longitudinal monitoring for therapeutic response and disease prognostication. These novel substrates, including supernatants from body fluids such as urine, pleural effusion, ascitic fluid, cerebrospinal fluid, as well as fine-needle aspiration (FNA) specimens, serve as a bridge between tissue-based testing and conventional liquid biopsy testing from the patient's plasma. Cytologically derived liquid biopsy samples can only be used in situations where the tissue sample is inadequate for genotyping, or when plasma-based liquid biopsy fails to identify an oncogenic driver alteration, but they can be used as a stand-alone complementary specimen source that can provide reliable genomic information for therapeutic decisions. This review aims to highlight some of the advances in the field and the clinical applications of the cytology-derived supernatant specimen.</p>","PeriodicalId":55187,"journal":{"name":"Cytopathology","volume":" ","pages":""},"PeriodicalIF":1.2,"publicationDate":"2025-02-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143375040","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Automated Description Generation of Cytologic Findings for Lung Cytological Images Using a Pretrained Vision Model and Dual Text Decoders: Preliminary Study.","authors":"Atsushi Teramoto, Ayano Michiba, Yuka Kiriyama, Tetsuya Tsukamoto, Kazuyoshi Imaizumi, Hiroshi Fujita","doi":"10.1111/cyt.13474","DOIUrl":"https://doi.org/10.1111/cyt.13474","url":null,"abstract":"<p><strong>Objective: </strong>Cytology plays a crucial role in lung cancer diagnosis. Pulmonary cytology involves cell morphological characterisation in the specimen and reporting the corresponding findings, which are extremely burdensome tasks. In this study, we propose a technique to generate cytologic findings from for cytologic images to assist in the reporting of pulmonary cytology.</p><p><strong>Methods: </strong>For this study, 801 patch images were retrieved using cytology specimens collected from 206 patients; the findings were assigned to each image as a dataset for generating cytologic findings. The proposed method consists of a vision model and dual text decoders. In the former, a convolutional neural network (CNN) is used to classify a given image as benign or malignant, and the features related to the image are extracted from the intermediate layer. Independent text decoders for benign and malignant cells are prepared for text generation, and the text decoder switches according to the CNN classification results. The text decoder is configured using a transformer that uses the features obtained from the CNN for generating findings.</p><p><strong>Results: </strong>The sensitivity and specificity were 100% and 96.4%, respectively, for automated benign and malignant case classification, and the saliency map indicated characteristic benign and malignant areas. The grammar and style of the generated texts were confirmed correct, achieving a BLEU-4 score of 0.828, reflecting high degree of agreement with the gold standard, outperforming existing LLM-based image-captioning methods and single-text-decoder ablation model.</p><p><strong>Conclusion: </strong>Experimental results indicate that the proposed method is useful for pulmonary cytology classification and generation of cytologic findings.</p>","PeriodicalId":55187,"journal":{"name":"Cytopathology","volume":" ","pages":""},"PeriodicalIF":1.2,"publicationDate":"2025-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143366759","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Navigating Diagnostic Uncertainty in Thyroid Nodules: The Critical Role of Cytology and Histology in Oncocytic and Rare Patterned Lesions.","authors":"Jaiden Townsend, Miguel Perez-Machado","doi":"10.1111/cyt.13473","DOIUrl":"https://doi.org/10.1111/cyt.13473","url":null,"abstract":"","PeriodicalId":55187,"journal":{"name":"Cytopathology","volume":" ","pages":""},"PeriodicalIF":1.2,"publicationDate":"2025-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143082242","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fine-Needle Aspiration Biopsy of Adrenal Gland Lesions: The Roles of Image Guidance, Rapid On-Site Evaluation and Additional Tissue Sampling.","authors":"Sanhong Yu, Darin Dolezal, Harry R Aslanian, Guoping Cai","doi":"10.1111/cyt.13472","DOIUrl":"https://doi.org/10.1111/cyt.13472","url":null,"abstract":"<p><strong>Objective: </strong>An accurate fine-needle aspiration (FNA) diagnosis of adrenal lesions may be challenging. This study was to investigate roles of imaging guidance, rapid on-site evaluation (ROSE) and additional tissue sampling in FNA diagnosis of adrenal lesions.</p><p><strong>Methods: </strong>Adrenal FNA cases were retrieved from pathology archive. Patients' demographics, lesion size and location, imaging guidance methods, cytologic diagnoses and histopathologic diagnoses were reviewed and analysed.</p><p><strong>Results: </strong>The study cohort included 72 cases of left (86%) and right (14%) adrenal lesions. Endoscopic ultrasound (EUS) and computed tomography (CT) were used in 47 (65%) and 25 (35%) cases, respectively. Left adrenal lesions were sampled mostly by EUS-FNA (73%), whereas right adrenal lesions by CT-guided FNA (80%). There were no differences between the EUS-FNA and CT-FNA groups in terms of non-diagnostic rate and cytologic diagnostic categories. The non-diagnostic rate and cytologic diagnostic categories were the same between ROSE and non-ROSE groups. In a subset of 18 cases with concurrent core tissue biopsy, a definite diagnosis was rendered in all biopsy cases including three cases with a non-diagnostic or indeterminate cytology diagnosis.</p><p><strong>Conclusion: </strong>Our study demonstrates that FNA has great efficacy for evaluation of adrenal lesions, either via EUS or CT guidance. Incorporation of ROSE evaluation into FNA procedure does not directly affect the performance of FNA biopsy but may help direct additional tissue sampling to salvage the cases with a non-diagnostic or indeterminate cytology diagnosis, increasing diagnostic yield.</p>","PeriodicalId":55187,"journal":{"name":"Cytopathology","volume":" ","pages":""},"PeriodicalIF":1.2,"publicationDate":"2025-01-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143061571","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Thy1c Cytology Is a Reliable Method of Excluding Thyroid Cancer in Dominant Cystic Nodules.","authors":"A Regan, A Boyd, N Patel, M J Stechman, D Scott-Coombes","doi":"10.1111/cyt.13471","DOIUrl":"https://doi.org/10.1111/cyt.13471","url":null,"abstract":"<p><strong>Background: </strong>Traditional teaching dictated that patients with recurrent thyroid cysts undergo excision owing to a 12% risk malignancy. Ultrasound evaluation now determines management of these patients augmented by fine needle biopsy. In UK, a non-diagnostic category for thyroid cysts (Thy1c) exists, whereas the Bethesda system combines 'non-diagnostic-cyst fluid only' into Category I along with paucicellular and acellular results. The aim was to assess the ability of Thy1c cytology to exclude malignancy.</p><p><strong>Methods: </strong>We undertook a retrospective study of patients undergoing thyroidectomy with Thy1c cytology from analysis of the Unit database. Additionally, we made a retrospective search in our pathology database for reports combining 'papillary thyroid carcinoma' with 'cyst' or 'cystic' and compared histology with cytology.</p><p><strong>Results: </strong>Between 2003 and 2023 115 patients (97 women, median age 44y, range 16-72) underwent thyroidectomy following Thy1c cytology. Indications for surgery included recurrent cyst (90%); compressive symptoms (9%) and one thyrotoxicosis. In no patient was the primary diagnosis malignant; multinodular goitre was commonest (87.76%); benign cyst (19.16%); follicular adenoma (5.4%) and coincidental pT1a classical papillary thyroid carcinoma (PTC) (4.3%). In the retrospective audit, 61 pathology reports contained 'PTC' and 'cyst'/'cystic'. The cystic element was in primary tumour (31.51%); nodal metastasis (17.28%) and adjacent benign disease (13.21%). Only 5 (8%) patients had Thy1c cytology; 4 had pT1a tumours and one a benign cyst and a 19 mm PTC.</p><p><strong>Conclusion: </strong>Thy1c category reliably excludes significant malignancy. For patients with symptomatic cysts, ultrasound assessment and Thy1c cytology can guide the clinician to treat with either ablation or resection without the fear of mistreating a thyroid cancer.</p>","PeriodicalId":55187,"journal":{"name":"Cytopathology","volume":" ","pages":""},"PeriodicalIF":1.2,"publicationDate":"2025-01-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143025760","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Proposal for Clinical Management of Nodules Diagnosed as Atypia of Undetermined Significance via Thyroid Fine-Needle Aspiration Cytology in the Absence of Molecular Testing","authors":"Ayana Suzuki,&nbsp;Mitsuyoshi Hirokawa,&nbsp;Makoto Kawakami,&nbsp;Takumi Kudo,&nbsp;Akira Miyauchi,&nbsp;Takashi Akamizu","doi":"10.1111/cyt.13467","DOIUrl":"10.1111/cyt.13467","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Objective</h3>\u0000 \u0000 <p>Molecular testing is recommended for risk stratification of atypia of undetermined significance (AUS) nodules in the USA; however, it is not routinely performed in some countries owing to limited availability and affordability. Here, we propose a risk stratification algorithm for AUS nodules when molecular testing is unavailable.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>We examined 304 (4.3%) AUS nodules among 7073 thyroid fine-needle aspiration cytology specimens examined at Kuma Hospital from January 2020 to December 2020. Clinical data were obtained from the medical records of Kuma Hospital.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>AUS with nuclear atypia and AUS-other each accounted for half of the total AUS nodules. The repeat aspiration rate was 19.7%; 61.7% of the nodules were reclassified as benign or malignant upon repeat aspiration. Resection rate and overall risk of malignancy (ROM) were 32.6% and 12.8%, respectively. Architectural atypia showed the lowest (1.1%) overall ROM in the AUS nodules. For AUS with nuclear atypia, nodules ≤ 10 mm in size showed significantly lower overall ROM than those of &gt; 10 mm, and nodules with ultrasonographically low suspicion showed significantly lower overall ROM than those with intermediate to high suspicion. AUS nodules with atypical lymphoid cells, possible medullary thyroid carcinoma, or possible parathyroid lesion were confirmed using flow cytometry, biochemical testing using needle washout fluid or immunocytochemistry, respectively.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>Our proposed clinical management algorithm for each subdivision according to cytological findings, based on repeat aspiration rates, ROM, ultrasound findings and results of ancillary tests except for molecular testing, should be useful for the clinical management of AUS nodules.</p>\u0000 </section>\u0000 </div>","PeriodicalId":55187,"journal":{"name":"Cytopathology","volume":"36 2","pages":"115-122"},"PeriodicalIF":1.2,"publicationDate":"2025-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/cyt.13467","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142967459","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Diagnostic Insighhts Into JC Virus-Infected Glial Cells: Intraoperative Cytopathological Analysis of Dot-Shaped Inclusions Associated With PML-NBs.","authors":"Nobuko Ujihira, Hideki Doi, Yukiko Shishido-Hara","doi":"10.1111/cyt.13469","DOIUrl":"https://doi.org/10.1111/cyt.13469","url":null,"abstract":"<p><p>This is the first case report describing the diagnostic value of dot-shaped inclusions associated with promyelocytic leukaemia nuclear bodies (PML-NBs) to define JC virus-infected glial cells in an intraoperative cytopathological diagnosis for progressive multifocal leukoencephalopathy (PML).</p>","PeriodicalId":55187,"journal":{"name":"Cytopathology","volume":" ","pages":""},"PeriodicalIF":1.2,"publicationDate":"2025-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142959038","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Study on the Transformation Process of Thyroid Fine-Needle Aspiration Liquid-Based Cytology to Whole-Slide Image","authors":"Yuanyuan Lei,&nbsp;Dongcun Wang,&nbsp;Yanlin Wen,&nbsp;Jinhui Liu,&nbsp;Jian Cao","doi":"10.1111/cyt.13468","DOIUrl":"10.1111/cyt.13468","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Objective</h3>\u0000 \u0000 <p>Analyse and summarise the reasons for failure in the digital acquisition of thyroid liquid-based cytology (LBC) slides and the technical challenges, and explore methods to obtain reliable and reproducible whole digital slide images for clinical thyroid cytology.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Method</h3>\u0000 \u0000 <p>Use the glass slide scanning imaging system to acquire whole-slide image (WSI) of thyroid LBC in sdpc format through different. Statistical analysis was conducted on the different acquisition methods, the quality of the glass slides, clinical and pathological characteristics of the case, TBSRTC grading and the quality of WSI.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>The WSI obtained by different scanning methods showed a high level of consistency in quality (<i>W</i> = 0.325, <i>p</i> &lt; 0.001), especially between fully automatic scanning with different focus densities (<i>W</i> = 0.9, <i>p</i> &lt; 0.001). A total of 2114 images were obtained through different methods of multi-layer fusion and multi-point focusing scanning, with scan success rates of 100.0%, 100.0%, 100.0% and 23.6%, respectively. The correlation between the quality of thyroid LBC glass slides and the image quality of thyroid LBC WSI was statistically significant (<i>p</i> &lt; 0.001). The correlation between TBSRTC grading and the quality of thyroid LBC digital WSI was statistically significant (<i>p</i> &lt; 0.001).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>Although the quality of glass slides has a significant impact, the success rate and image quality of malignant tumour scanning are both high. Overall, the risk of missed diagnosis of malignant tumours is low. In the future, we also need to improve the performance and algorithm of the scanner in cases of sparse cells.</p>\u0000 </section>\u0000 </div>","PeriodicalId":55187,"journal":{"name":"Cytopathology","volume":"36 2","pages":"106-114"},"PeriodicalIF":1.2,"publicationDate":"2025-01-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/cyt.13468","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142959052","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cytological Features of Sialoblastoma: A Case Report With Summary of Prior Published Cases","authors":"Firdous Wasim Sk,&nbsp;Debalina Karmakar,&nbsp;Urvee Sarkar,&nbsp;Dipak Ghosh,&nbsp;Uttara Chatterjee","doi":"10.1111/cyt.13466","DOIUrl":"10.1111/cyt.13466","url":null,"abstract":"<p>Cytological diagnosis of sialoblastoma can be challenging. This report describes describe the cytological findings of sialoblastoma in an infant along with its cytological differentials and a summary of prior published cases. Cellular smears composed of small round basaloid cells with occasional ductal formation were the main features.</p>","PeriodicalId":55187,"journal":{"name":"Cytopathology","volume":"36 2","pages":"175-179"},"PeriodicalIF":1.2,"publicationDate":"2024-12-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142883673","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}