Performance of MammaTyper in Fine Needle Aspiration Cytology, Core Needle Biopsy and Surgical Breast Cancer Samples.

IF 1.1 4区医学 Q4 CELL BIOLOGY

Cytopathology Pub Date : 2025-09-24 DOI:10.1111/cyt.70026

Maurizio Pinamonti, Daniela Santon, Cristina Bottin, Elisa Ober, Clara Rizzardi, Claudia Di Lecce, Fabiola Giudici, Jerry Polesel, Fabrizio Zanconati

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引用次数: 0

Abstract

Objective: To evaluate the performance and concordance of the MammaTyper molecular test across cytological, biopsy and surgical samples from breast carcinomas, emphasising the applicability of cytology in molecular subtyping.

Methods: 29 breast carcinoma cases, each represented by cytological, biopsy and surgical samples, were retrospectively collected from 2021 to 2025 at the Pathology Unit of Trieste. Subtyping was performed using MammaTyper on all sample types. Concordance between MammaTyper results across different sample types was assessed using Lin's concordance correlation coefficient (CCC), overall concordance rate (OCR) and kappa statistics.

Results: A total of 84 samples from breast carcinoma were analysed, including FNAC, CNB and surgical resection specimens. Molecular subtyping was technically successful in all cases. When comparing IHC and MammaTyper results retrieved in CNBs, we found almost perfect agreement for ER, substantial agreement for PgR and HER2 and moderate agreement for Ki-67. Concordance rates between cytology and FFPE samples (respectively CNB and surgical specimens) showed excellent agreement for ER (CCC 0.81 and 0.835), good agreement for PgR (0.714 and 0.78), excellent agreement for HER2 (0.855 and 0.854) and moderate agreement for Ki-67 (0.626 and 0.643). In particular, discrepancies in HER2 and Ki-67 status between cytological and FFPE samples were observed in a relevant subset of cases, potentially affecting therapeutic stratification.

Conclusions: MammaTyper demonstrated high concordance and reliability on cytological and biopsy samples, supporting its use in minimally invasive diagnostics. Despite the discrepancies in some markers, cytology offers a cost-effective, less invasive alternative to biopsies for molecular subtyping, aligning with precision medicine goals.

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MammaTyper在细针穿刺细胞学、核心针活检和手术乳腺癌样本中的表现。

目的：评价MammaTyper分子检测在乳腺癌细胞学、活检和手术样本中的表现和一致性，强调细胞学在分子分型中的适用性。方法：回顾性收集的里雅斯特病理科2021年至2025年29例乳腺癌病例，每例均有细胞学、活检和手术标本。使用MammaTyper对所有样本类型进行分型。采用Lin’s Concordance correlation coefficient （CCC）、overall Concordance rate （OCR）和kappa统计量评估MammaTyper结果在不同样本类型之间的一致性。结果：共分析84例乳腺癌标本，包括FNAC、CNB和手术切除标本。在所有病例中，分子分型在技术上都是成功的。当比较IHC和MammaTyper在CNBs中检索到的结果时，我们发现ER的结果几乎完全一致，PgR和HER2的结果基本一致，Ki-67的结果中等一致。细胞学和FFPE样本（分别为CNB和手术标本）的一致性率显示，ER的一致性很好（CCC 0.81和0.835），PgR的一致性很好（0.714和0.78），HER2的一致性很好（0.855和0.854），Ki-67的一致性中等（0.626和0.643）。特别是，在一些相关的病例中，细胞学和FFPE样本之间的HER2和Ki-67状态存在差异，这可能会影响治疗分层。结论：MammaTyper在细胞学和活检样本上显示出高度的一致性和可靠性，支持其在微创诊断中的应用。尽管在一些标记上存在差异，但细胞学为分子分型提供了一种成本效益高、侵入性较小的替代活检方法，与精准医学目标保持一致。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Cytopathology 生物-病理学

CiteScore

2.30

自引率

15.40%

发文量

107

审稿时长

6-12 weeks

期刊介绍： The aim of Cytopathology is to publish articles relating to those aspects of cytology which will increase our knowledge and understanding of the aetiology, diagnosis and management of human disease. It contains original articles and critical reviews on all aspects of clinical cytology in its broadest sense, including: gynaecological and non-gynaecological cytology; fine needle aspiration and screening strategy. Cytopathology welcomes papers and articles on: ultrastructural, histochemical and immunocytochemical studies of the cell; quantitative cytology and DNA hybridization as applied to cytological material.