Intercenter Study of Interobserver Variability With Optical and Digital Imaging in Cervicovaginal Cytology. Simulation of a Quality Control Program.

IF 1.1 4区医学 Q4 CELL BIOLOGY

Cytopathology Pub Date : 2025-09-19 DOI:10.1111/cyt.70025

Francesc Tresserra, Carme Dinarès, Olga Luque, Marta Serra, Miriam Castella, Lucia Retamales, Gemma Fabra, Santiago Valera, Ignacio Rodríguez

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引用次数: 0

Abstract

Introduction: Participation in diagnostic intercomparison programs is a mandatory requirement for accreditation of cytology laboratories. Results described by these programs in cervico vaginal cytology (CVC) show a significant number of discrepancies. We hypothesised whether the use of IA systems, such as the ThinPrep Genius system, would improve diagnostic concordance.

Methods: We simulated an intercomparison program between two centres in which four observers used the same 30 cases of CVC with negative diagnoses, ASCUS, LSIL and HSIL and observed them with light optic microscopy (OM) and two Genius (G1 and G2) systems, one from each centre.

Results: The diagnostic agreement between OM and the two Genius was similar, with overall agreement values of 82% for G1 and 78% for G2 and OM, and similar kappa values for the three techniques. However, both Genius diagnosed glandular lesions that were not included in the selected cases and were not diagnosed with OM.

Conclusion: The concordance obtained in both OM and Genius is acceptable and falls within the range of that obtained in what is described in intercomparison programs. We must consider the greater affinity for diagnosing glandular lesions with Genius and the fact that there may be discrete differences between two different devices, probably due to calibration.

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宫颈阴道细胞学中光学和数字成像的观察者间变异性的中心间研究。质量控制程序的模拟。

简介：参与诊断比较计划是细胞学实验室认证的强制性要求。结果描述了这些程序在宫颈阴道细胞学（CVC）显示显著数量的差异。我们假设使用IA系统，如ThinPrep Genius系统，是否会提高诊断一致性。方法：我们模拟了两个中心之间的相互比较程序，其中四名观察员使用相同的30例阴性诊断的CVC， ASCUS， LSIL和HSIL，并使用光学显微镜（OM）和两个Genius （G1和G2）系统进行观察，每个中心一个。结果：OM和两种Genius的诊断一致性相似，G1的总体一致性值为82%，G2和OM的总体一致性值为78%，三种技术的kappa值相似。然而，Genius诊断出的腺体病变并未包括在选定的病例中，也未被诊断为OM。结论：在OM和Genius中获得的一致性是可以接受的，并且落在相互比较程序中所描述的一致性范围内。我们必须考虑Genius在诊断腺体病变方面的更大亲和力，以及两种不同设备之间可能存在离散差异的事实，这可能是由于校准所致。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Cytopathology 生物-病理学

CiteScore

2.30

自引率

15.40%

发文量

107

审稿时长

6-12 weeks

期刊介绍： The aim of Cytopathology is to publish articles relating to those aspects of cytology which will increase our knowledge and understanding of the aetiology, diagnosis and management of human disease. It contains original articles and critical reviews on all aspects of clinical cytology in its broadest sense, including: gynaecological and non-gynaecological cytology; fine needle aspiration and screening strategy. Cytopathology welcomes papers and articles on: ultrastructural, histochemical and immunocytochemical studies of the cell; quantitative cytology and DNA hybridization as applied to cytological material.