Flora最新文献

{"title":"Standardization of two forms of the CRENATE (Pooideae Benth.) phytolith morphotype and their potential for environmental reconstructions","authors":"Marina Solomonova ,&nbsp;Mikhail Blinnikov ,&nbsp;Natalya Speranskaya ,&nbsp;Alena Lyashchenko ,&nbsp;Sergey Kotov ,&nbsp;Natalya Elesova ,&nbsp;Maksim Borisenko ,&nbsp;Marina Silantyeva","doi":"10.1016/j.flora.2024.152646","DOIUrl":"10.1016/j.flora.2024.152646","url":null,"abstract":"<div><div>The separation of two forms of <span>Crenate</span> phytoliths from the epidermal short cells of Pooideae subfamily is proposed here for the first time, based on vegetation composition and climatic data. We examined 210 topsoil samples from 70 vegetation plots in about 12 plant communities from the Russian Altai mountains to detect differences in the frequencies of occurrence of <span>Crenate sinuate</span> (CRE_SIN) and C<span>renate lobate</span> (CRE_LOB) submorphotypes. A ratio of the frequencies of these two phytoliths (s/l) is proposed. We clarified the difference between C<span>renate sinuate</span> and C<span>renate lobate</span> using morphometry of two target grass species. C<span>renate lobate</span> has been studied in the leaves of <em>Dactylis glomerata</em> L. C<span>renate sinuate</span> has been studied in the leaves of <em>Koeleria cristata</em> L. Our study has shown that the frequencies of CRE_SIN, CRE_LOB and s/l ratio depend on the temperature of the coldest and warmest months of the year and the amount of annual precipitation. When counting <span>Crenate</span> as a single morphotype, however, the relationship with climate variables is much weaker. Two submorphotypes have different associations with moisture conditions. Morphometric studies of CRE_SIN and CRE_LOB and data analysis revealed that the most important parameters for separating these two morphotypes are aspect ratio, elongation, convexity and solidity. Distinction between these two morphotypes may aid paleoenvironmental and ecological research in the temperate regions of the world, where <span>Crenate</span> phytoliths of Pooideae are very common.</div></div>","PeriodicalId":55156,"journal":{"name":"Flora","volume":"322 ","pages":"Article 152646"},"PeriodicalIF":1.7,"publicationDate":"2024-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142746119","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Heteroblasty in Conchocarpus heterophyllus (A.St.-Hil.) Kallunki & Pirani (Rutaceae): An approach of leaf development from the unifoliolate leaves","authors":"Carlos E.V. Raymundo,&nbsp;José R. Pirani,&nbsp;Gladys F.A. Melo-de-Pinna","doi":"10.1016/j.flora.2024.152645","DOIUrl":"10.1016/j.flora.2024.152645","url":null,"abstract":"<div><div>Heteroblasty is a phenomenon marked by abrupt changes in the development of organs of certain species. In our research on <em>Conchocarpus heterophyllus</em>, a shrub with palmately arranged leaflets ranging from 3(4) to 5(<strong>–</strong>7), we observed significant variations in leaf patterns between seedlings, young plants, and plants in the reproductive phase, indicating transition events during plant development. The focus of this study is to thoroughly investigate these events of heteroblasty and heterophylly in <em>C. heterophyllus</em>, with new evidence of the unifoliolate condition in Rutaceae. Morphological and anatomical observations were conducted from mature leaves of individuals at different stages of development<em>.</em> The results show that the heteroblasty in <em>C. heterophyllus</em> is marked by changes in leaf patterns across three stages. In stage 1, seedlings exclusively produce unifoliolate leaves from the first foliage leaf. After the eighth to tenth node from the cotyledons, heterophylly becomes evident with the emergence of leaves containing 2<strong>–</strong>3 leaflets, in addition to unifoliolate leaves, characterizing the second stage. The third stage is defined by the onset of the reproductive phase, marked by the presence of inflorescences. In this stage, the leaf complexity varies, with the number of leaflets ranging from 2 to 5, and unifoliolate leaves are lacking. Furthermore, our study identified the occurrence of vascular traces of leaflets in the swollen region located at the apex of the petiole of all unifoliolate leaves, occurring in stages 1 and 2. These vascular traces are interpreted as remnants of the lost lateral leaflets in unifoliolate leaves and represent novel evidence of this special condition in compound leaves. Heteroblasty in <em>C. heterophyllus</em> is a genetically controlled phenomenon, and consequently, prospective investigations involving microRNAs, particularly microRNAs 156 and 172, have the potential to provide valuable insights into the genetic mechanisms underlying the transition between different phases.</div></div>","PeriodicalId":55156,"journal":{"name":"Flora","volume":"322 ","pages":"Article 152645"},"PeriodicalIF":1.7,"publicationDate":"2024-11-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142722460","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bioclimatic variables affect seed mass and seedling traits of four Gymnocalycium (Cactaceae) species along elevational gradients","authors":"Karen Bauk ,&nbsp;Nicola Rossi ,&nbsp;Diego E. Gurvich ,&nbsp;M. Laura Las Peñas","doi":"10.1016/j.flora.2024.152643","DOIUrl":"10.1016/j.flora.2024.152643","url":null,"abstract":"<div><div>Differences in climatic factors associated with elevation can lead to natural selection for local adaptation in species with a wide distribution range, with some regenerative characteristics changing along the gradient. Most Cactaceae species occur in heterogeneous environments, mainly in mountains. The increasing severity of environmental conditions along elevational gradients could affect seed size, influencing seedling growth. The main aims of the study were to analyse whether seed mass, and seedling size and shape are related to elevational gradients in different populations of four species of the genus <em>Gymnocalycium</em> and to evaluate the relationship between seed mass and bioclimatic variables. Populations of four species of <em>Gymnocalycium</em> (<em>G. andreae, G. erinaceum, G. monvillei</em> and <em>G. mostii</em>) were sampled; the populations were located along three different elevation gradients, with each population corresponding to an elevational class (extremes and intermediate sites). At each elevational site, mature fruits were collected and seed mass and seedling traits were measured. A shape index was calculated by dividing height by width. A value of 1 represents 'globose' seedlings, while increasing values indicate that the seedlings become 'columnar'. The Pearson correlation coefficient was calculated to analyse the relationship between seed mass and seedling size variables. Linear models were used to analyse seed size and seedling shape. First, with elevation as a predictor and then for seed mass, the 19 bioclimatic variables were extracted from WorldClim. The results show that seed mass was positively correlated with seedling shape index, and with seedling height and width. Differences in seed mass among populations were explained by the species and the gradient where they lived, as well as by the environmental variables mean temperature of the coldest quarter, precipitation of the wettest month, and precipitation of the driest month. In conclusion, the intraspecific analyses of the four <em>Gymnocalycium</em> species revealed that the seedling shape patterns were similar across all species in relation to elevation. However, seed mass varied with elevation differently for each species. These findings suggest that each species possesses unique characteristics that enable them to thrive in varying climatic conditions along elevational gradients.</div></div>","PeriodicalId":55156,"journal":{"name":"Flora","volume":"322 ","pages":"Article 152643"},"PeriodicalIF":1.7,"publicationDate":"2024-11-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142697446","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biosystematics studies of endemic Thlaspi violascens Boiss. and T. densiflorum Boiss. & Kotschy: Highlights on molecular and morphological identification","authors":"Mehmet Cengiz Karaismailoğlu ,&nbsp;Vahap Eldem ,&nbsp;Behcet İnal ,&nbsp;Mehmet Ali Balcı","doi":"10.1016/j.flora.2024.152644","DOIUrl":"10.1016/j.flora.2024.152644","url":null,"abstract":"<div><div>This study focuses on differentiating the endemic species <em>Thlaspi violascens</em> and <em>T. densiflorum</em>, which exhibit similar macromorphological characteristics and population appearances with utilizing molecular, anatomical, and micromorphological methods. The complete chloroplast genomes of both species have been sequenced for the first time using next-generation sequencing technologies, allowing for high-resolution analysis of their phylogenetic relationships. The isolated chloroplast genomes measure 152,693 base pairs for <em>T. violascens</em> and 152,718 base pairs for <em>T. densiflorum</em>. Phylogenetic analysis shows that species within the <em>Thlaspi</em> genus are monophyletically distinct from other genera. Although the average nucleotide diversity between the two species is low, whole-genome sequencing-based Skmer analysis has successfully enabled high-resolution species-level discrimination. Anatomically, the two species differ in several aspects, including the type of petiole tip, abaxial surface outline, epidermal cell size and layer count, petiole thickness, vascular bundle number, midrib lobes, mesophyll cell types, and collenchyma layer count. Micromorphological differences are also evident in the primary structures of the anther and ovary, as well as the secondary structures of the sepal and style. Overall, the study concludes that chloroplast genome data, petiolar anatomical features, and floral micromorphological traits can effectively distinguish between <em>T. violascens</em> and <em>T. densiflorum.</em></div></div>","PeriodicalId":55156,"journal":{"name":"Flora","volume":"322 ","pages":"Article 152644"},"PeriodicalIF":1.7,"publicationDate":"2024-11-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142697443","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The phylogenetic context of corolla symmetry in Canscora Lam. (Gentianaceae)","authors":"Aditi Sarawgi ,&nbsp;Milind Madhav Sardesai ,&nbsp;Ritesh Kumar Choudhary","doi":"10.1016/j.flora.2024.152641","DOIUrl":"10.1016/j.flora.2024.152641","url":null,"abstract":"<div><div>Floral symmetries are perceived as an essential structural trait for classification in angiosperms. However, the traditional view of floral symmetries considered only two states—actinomorphic or zygomorphic—often disregarding other forms. This binary view can be onerous, as floral development may involve numerous shifts in symmetry. In the Gentianaceae family, zygomorphy is predominantly regarded as a synapomorphy for <em>Canscora</em>, leading to the segregation of some actinomorphic <em>Canscora</em> species into the separate genus <em>Canscorinella</em>. Additionally, species with slightly zygomorphic forms have been reported, leaving the relationships among various floral symmetries largely unaddressed. To investigate the phylogenetic relations of floral symmetries in <em>Canscora</em>, we analyzed DNA sequence data from nuclear and plastid markers and examined the generic status of <em>Canscorinella</em>. Furthermore, an ontogenetic study was conducted to understand the ancestral floral symmetries in <em>Canscora</em>. Our analyses revealed the independent evolution of zygomorphy in Gentianaceae and the reversal of actinomorphy in <em>Canscora</em>. This study provided insights into the phylogenetic relationship of <em>Canscorinella</em> within the subtribe <em>Canscorinae</em>, suggesting that <em>Canscora</em> exhibits actinomorphy in its early growth stages, which later develops into zygomorphy.</div></div>","PeriodicalId":55156,"journal":{"name":"Flora","volume":"322 ","pages":"Article 152641"},"PeriodicalIF":1.7,"publicationDate":"2024-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142697445","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Orbicules in south American Balanophoraceae: Analysis of its possible role and its taxonomic value in the family","authors":"Virginia Cristina Gómez Villafañe ,&nbsp;Héctor Arnaldo Sato ,&nbsp;Diego Demarco ,&nbsp;Ana María Gonzalez","doi":"10.1016/j.flora.2024.152642","DOIUrl":"10.1016/j.flora.2024.152642","url":null,"abstract":"<div><div>Balanophoraceae are root parasites, lacking both chlorophyll and typical cormophytic organization. There have been few studies of pollen dispersal mechanisms in Balanophoraceae, and no studies on structures accompanying pollen, such as orbicules. Anthers were analyzed in five South American taxa of Balanophoraceae (<em>Langsdorffia hypogaea, Lophophytum pyramidale, L. mirabile</em>. subsp. <em>bolivianum, Ombrophytum subterraneum</em> and <em>Helosis cayennensis</em>), in order to: (1) determine the presence of orbicules, characterize them and relate their presence with the tapetum type, characteristics of the anther wall and dehiscence, and the pollination mode; (2) and analyze its possible taxonomic value in relation to the phylogeny of the family Balanophoraceae. In three genera (<em>Lophophytum, Langsdorffia</em> and <em>Ombrophytum</em>), the anthers were found to be dithecous, tetrasporangiate with longitudinal dehiscence. In these taxa the endothecium is present and is responsible for dehiscing the anthers. In <em>Helosis</em> the pollen sacs are welded in a synandrium with apical groves and lacking an endothecium. In all species, a secretory tapetum is responsible for nourishing the pollen grains during their formation. The presence of orbicules is variable among the species studied: it is found in <em>Lophophytum</em> and <em>Langsdorffia</em>, and is absent in <em>Ombrophytum</em> and <em>Helosis</em>. In <em>Lophophytum</em> the orbicules appear as small and spheroidal corpuscles with smooth surfaces. In <em>Langsdorffia hypogaea</em> the orbicules are conspicuous spherical, ellipsoid to ovoid forms. This study shows that there is no relationship between layers present in mature anthers, dehiscence type, pollination mode and orbicules presence. We report for the first time the presence of orbicules in the holoparasitic family Balanophoraceae belonging to the order Santalales, in which there is scarce information available regarding the presence of orbicules. Given the intergeneric variations and the consistency at generic level observed in the taxa here analyzed, orbicules may have predictive value and potential for systematically oriented research questions.</div></div>","PeriodicalId":55156,"journal":{"name":"Flora","volume":"321 ","pages":"Article 152642"},"PeriodicalIF":1.7,"publicationDate":"2024-11-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142660115","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analysis of phytolith inputs from natural plant communities and crops and soil silicon availability (Southeastern Pampean region, Argentina)","authors":"María Laura Benvenuto ,&nbsp;Mara De Rito ,&nbsp;Margarita Luisa Osterrieth ,&nbsp;Mariana Fernández Honaine","doi":"10.1016/j.flora.2024.152640","DOIUrl":"10.1016/j.flora.2024.152640","url":null,"abstract":"<div><div>Silica phytoliths constitute an important source of Si to terrestrial environments. Different plant communities contribute varying amounts of phytoliths to soils due to differences in plant taxa. In Pampean region, Argentina, agriculture has largely replaced natural communities like grasslands and wetlands. This replacement affects the specific plant composition of the area, modifies the potential phytolith input from plants to soils, and as a consequence, the soil Si content. Since no study specifically measures the potential phytolith contribution from natural communities and crops in the region, we aimed to quantify and compare phytolith production in natural plant communities and typical crops from Pampean region and its relation to available soil Si content. Three natural communities and three crops were selected. Phytolith content in plant communities was estimated by the phytolith amount produced in plant tissues (calcination technique) and plant biomass, while soil Si was measured by UV–VIS spectrophotometry. Phytolith content differed between natural communities and crops. The communities with the highest phytolith content were those that contained high phytolith-producing species and / or high plant biomass (959.61 ± 95.64 and 665.27 ± 382.38 kg/ha, wheat and \"juncal\", respectively). There were no significant differences in Si content between natural and cropland soils, and phytolith production differences in plant communities were not reflected in the soils. Probably soil genesis, vegetation history and / or management can explain the lack of differences. Continuous monitoring of phytolith production, plant biomass and soil Si content could explain the Si cycle in this region.</div></div>","PeriodicalId":55156,"journal":{"name":"Flora","volume":"322 ","pages":"Article 152640"},"PeriodicalIF":1.7,"publicationDate":"2024-11-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142697444","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Journey to the west: Migration patterns of the riparian montane genus Myricaria","authors":"Kailin Sun,&nbsp;Silke Werth","doi":"10.1016/j.flora.2024.152639","DOIUrl":"10.1016/j.flora.2024.152639","url":null,"abstract":"<div><div><em>Myricaria</em> Desv. (Tamaricaceae) is a genus of riparian plants found in montane regions throughout Eurasia. We present molecular phylogenies covering the entire Eurasian range of the genus using the chloroplast interspacer region <em>psb</em>A-<em>trn</em>H, the chloroplast region <em>trn</em>L-<em>trn</em>F, and the nuclear ribosomal Internal Transcribed Spacer (ITS). Biogeographical analyses of the DNA sequencing data based on Takhtajan's Floristic Provinces using a Bayesian Binary Markov Chain Monte Carlo reconstruction show that the origin of <em>Myricaria</em> is the Tibetan Floristic Province, specifically in the Qinghai-Tibetan Plateau. A molecular clock analysis was also performed. We estimate that the crown age of <em>Myricaria</em> is around 22.8 Ma and that the common ancestor of all European <em>Myricaria</em> occurred around 8.8 Ma ago. By covering the entire native range of the genus, our analyses confirm previous hypotheses on the origin and migration patterns of <em>Myricaria</em>, namely a central Asian centre of origin, followed by a westward migration to Europe via the central Asian mountain ranges, the Caucasus, and Eastern Europe. The divergence times and migration routes of <em>Myricaria</em> are remarkably similar to that of the genus <em>Hippophae</em> L<em>.</em></div></div>","PeriodicalId":55156,"journal":{"name":"Flora","volume":"321 ","pages":"Article 152639"},"PeriodicalIF":1.7,"publicationDate":"2024-11-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142659601","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Geographical and environmental influences on Boswellia elongata Balf.f. volatiles: An in situ study on Socotra Island","authors":"Jana Tulková ,&nbsp;Antonio Pompeiano ,&nbsp;Tara Joy Massad ,&nbsp;Petr Vahalík ,&nbsp;Zuzana Paschová ,&nbsp;Lucie Vaníčková ,&nbsp;Petr Maděra","doi":"10.1016/j.flora.2024.152638","DOIUrl":"10.1016/j.flora.2024.152638","url":null,"abstract":"<div><div>Frankincense, an esteemed oleo-gum resin derived from <em>Boswellia</em> species (Burseraceae), has been prized by humans for its aromatic, medicinal, and spiritual properties across millennia. Environmental factors play a crucial role in shaping the volatile compound composition of this resin, thereby influencing its quality. In this investigation, we analyzed frankincense sourced from <em>Boswellia elongata</em> Balf.f. at six distinct locations on Socotra Island. Employing thermal desorption-gas chromatography coupled with mass spectrometry (TD-GC/MS), we identified 92 terpenoid compounds, spanning monoterpenes, sesquiterpenes, and diterpenes. Predominant among these volatile organic compounds (VOCs) were α-pinene, γ-terpinene, o-cymene, and (<em>E</em>)-β-farnesene. Multivariate analyses revealed subtle differences in VOC composition, particularly pronounced at the Ma'aleh site, the most geographically isolated research locality. The flowers in this population also displayed distinct red-pink coloration compared to other populations. Integration of VOC data with environmental variables revealed correlations with temperature, elevation, and proximity to the sea, with specific compounds exhibiting associations with distinct environmental contexts. Our findings underscore the impact of microclimatic and abiotic conditions on the volatile profile of individual trees. Moreover, this study offers comprehensive insights into the VOC profile of <em>B. elongata</em> resin and its interplay with environmental factors, thereby suggesting avenues for the cultivation of new varieties or hybridization within the species.</div></div>","PeriodicalId":55156,"journal":{"name":"Flora","volume":"321 ","pages":"Article 152638"},"PeriodicalIF":1.7,"publicationDate":"2024-11-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142659603","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anatomical, histochemical and micrographic analysis of aerial parts of Aristolochia elegans Mast. (Aristolochiaceae), a potentially toxic plant used in folk medicine","authors":"Ignacio J. Agudelo ,&nbsp;María Victoria Cura ,&nbsp;Cecilia B. Dobrecky ,&nbsp;Leonardo M. Anconatani ,&nbsp;María Inés Mercado ,&nbsp;Marcelo L. Wagner ,&nbsp;Rafael A. Ricco","doi":"10.1016/j.flora.2024.152626","DOIUrl":"10.1016/j.flora.2024.152626","url":null,"abstract":"<div><div><em>Aristolochia elegans</em> (Aristolochiaceae) is a plant species indigenous to South America, widely employed in traditional folk medicine and commonly referred to as 'mil hombres'. Despite its medicinal applications, this plant contains aristolochic acid; a compound associated with the potential risk of chronic intoxications and induced nephropathy following prolonged ingestion. The absence of comprehensive microscopic identification guidelines for <em>A. elegans</em>, whether utilizing fresh, dried, or powdered specimens, highlights the necessity to establish its pharmacognostic diagnostic characteristics ensuring the recognition of the species and its safe and controlled utilization.</div><div>Mild dissociations, transversal sections and electron microscopy observations were performed on specimens collected at the Costanera Sur Ecological Reserve and the Buenos Aires Botanical Garden (CABA). In addition, histochemical tests were conducted to asess the presence of primary and secondary metabolites.</div><div>The stems exhibited secondary growth, characterized by diffuse porous wood and 7–9 medullary rays. The xylem showed wide scalariform pitted vessel units and fibrotracheids. Whereas the leaves presented hooked trichomes and rodlet-shaped epicuticular wax deposits, a common trait among <em>Aristolochia</em> species. The histochemical analysis revealed the presence of alkaloids in the cortical parenchyma and fibers of the stems and petioles, as well as phenolic compounds in the xylem and cortical parenchyma of young stem and in the lamina mesophyll and abaxial epidermis adjacent to the midrib of the leaf. These micrographic described features serve as valuable tools for identifying <em>A. elegans</em> in commercial samples.</div></div>","PeriodicalId":55156,"journal":{"name":"Flora","volume":"321 ","pages":"Article 152626"},"PeriodicalIF":1.7,"publicationDate":"2024-11-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142659602","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}