Fungal Genetics and Biology最新文献_第9页

Update on the state of research to manage Fusarium head blight 枯萎病防治研究进展。

IF 3 3区生物学

Fungal Genetics and Biology Pub Date : 2023-09-02 DOI: 10.1016/j.fgb.2023.103829

Soumya Moonjely , Malaika Ebert , Drew Paton-Glassbrook , Zachary A. Noel , Ludmila Roze , Rebecca Shay , Tara Watkins , Frances Trail

引用次数: 0

A reference transcriptome for walnut anthracnose pathogen, Ophiognomonia leptostyla, guides the discovery of candidate virulence genes 核桃炭疽病病原菌细端线虫(Ophiognomonia leptostyla)的参考转录组指导候选毒力基因的发现

IF 3 3区生物学

Fungal Genetics and Biology Pub Date : 2023-08-31 DOI: 10.1016/j.fgb.2023.103828

Fatemeh Khelghatibana , Mohammad Javan-Nikkhah , Naser Safaie , Ahmad Sobhani , Somayeh Shams , Ehsan Sari

{"title":"A reference transcriptome for walnut anthracnose pathogen, Ophiognomonia leptostyla, guides the discovery of candidate virulence genes","authors":"Fatemeh Khelghatibana , Mohammad Javan-Nikkhah , Naser Safaie , Ahmad Sobhani , Somayeh Shams , Ehsan Sari","doi":"10.1016/j.fgb.2023.103828","DOIUrl":"10.1016/j.fgb.2023.103828","url":null,"abstract":"<div>Despite the economic losses due to the walnut anthracnose, Ophiognomonia leptostyla is an orphan fungus with respect to genomic resources. In the present study, the transcriptome of O. leptostyla was assembled for the first time. RNA sequencing was conducted for the fungal mycelia grown in a liquid media, and the inoculated leaf samples of walnut with the fungal conidia sampled at 48, 96 and 144 h post inoculation (hpi). The completeness, correctness, and contiguity of the de novo transcriptome assemblies generated with Trinity, Oases, SOAPdenovo-Trans and Bridger were compared to identify a single superior reference assembly. In most of the assessment criteria including N50, Transrate score, number of ORFs with known description in gene bank, the percentage of reads mapped back to the transcript (RMBT), BUSCO score, Swiss-Prot coverage bin and RESM-EVAL score, the Bridger assembly was the superior and thus used as a reference for profiling the O. leptostyla transcriptome in liquid media vs. during walnut infection. The k-means clustering of transcripts resulted in four distinct transcription patterns across the three sampling time points. Most of the detected CAZy transcripts had elevated transcription at 96 hpi that is hypothetically concurrent with the start of intracellular growth. The in-silico analysis revealed 103 candidate effectors of which six were members of Necrosis and Ethylene Inducing Like Protein (NLP) gene family belonging to three distinct k-means clusters. This study provided a complex and temporal pattern of the CAZys and candidate effectors transcription during six days post O. leptostyla inoculation on walnut leaves, introducing a list of candidate virulence genes for validation in future studies.</div>","PeriodicalId":55135,"journal":{"name":"Fungal Genetics and Biology","volume":"169 ","pages":"Article 103828"},"PeriodicalIF":3.0,"publicationDate":"2023-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10283788","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Bcvic1 and Bcvic2 vegetative incompatibility genes in Botrytis cinerea encode proteins with domain architectures involved in allorecognition in other filamentous fungi 灰葡萄孢中的Bcvic1和Bcvic2营养不亲和基因编码的蛋白质具有与其他丝状真菌中的同种异体识别有关的结构域结构

IF 3 3区生物学

Fungal Genetics and Biology Pub Date : 2023-08-26 DOI: 10.1016/j.fgb.2023.103827

Saadiah Arshed , Murray P. Cox , Ross E. Beever , Stephanie L. Parkes , Michael N. Pearson , Joanna K. Bowen , Matthew D. Templeton

{"title":"The Bcvic1 and Bcvic2 vegetative incompatibility genes in Botrytis cinerea encode proteins with domain architectures involved in allorecognition in other filamentous fungi","authors":"Saadiah Arshed , Murray P. Cox , Ross E. Beever , Stephanie L. Parkes , Michael N. Pearson , Joanna K. Bowen , Matthew D. Templeton","doi":"10.1016/j.fgb.2023.103827","DOIUrl":"10.1016/j.fgb.2023.103827","url":null,"abstract":"<div>Vegetative incompatibility is a fungal allorecognition system characterised by the inability of genetically distinct conspecific fungal strains to form a viable heterokaryon and is controlled by multiple polymorphic loci termed vic (vegetative incompatibility) or het (heterokaryon incompatibility). We have genetically identified and characterised the first vic locus in the economically important, plant-pathogenic, necrotrophic fungus Botrytis cinerea. A bulked segregant approach coupled with whole genome Illumina sequencing of near-isogenic lines of B. cinerea was used to map a vic locus to a 60-kb region of the genome. Within that locus, we identified two adjacent, highly polymorphic open reading frames, Bcvic1 and Bcvic2, which encode predicted proteins that contain domain architectures implicated in vegetative incompatibility in other filamentous fungi. Bcvic1 encodes a predicted protein containing a putative serine esterase domain, a NACHT family of NTPases domain, and several Ankyrin repeats. Bcvic2 encodes a putative syntaxin protein containing a SNARE domain; such proteins typically function in vesicular transport. Deletion of Bcvic1 and Bcvic2 individually had no effect on vegetative incompatibility. However, deletion of the region containing both Bcvic1 and Bcvic2 resulted in mutant lines that were severely restricted in growth and showed loss of vegetative incompatibility. Complementation of these mutants by ectopic expression restored the growth and vegetative incompatibility phenotype, indicating that Bcvic1 and Bcvic2 are controlling vegetative incompatibility at this vic locus.</div>","PeriodicalId":55135,"journal":{"name":"Fungal Genetics and Biology","volume":"169 ","pages":"Article 103827"},"PeriodicalIF":3.0,"publicationDate":"2023-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10517355","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Detection and quantification of the histone code in the fungal genus Aspergillus 曲霉真菌属组蛋白密码的检测与定量

IF 3 3区生物学

Fungal Genetics and Biology Pub Date : 2023-06-01 DOI: 10.1016/j.fgb.2023.103800

Xin Zhang , Roberta Noberini , Alessandro Vai , Tiziana Bonaldi , Michael F. Seidl , Jérȏme Collemare

{"title":"Detection and quantification of the histone code in the fungal genus Aspergillus","authors":"Xin Zhang , Roberta Noberini , Alessandro Vai , Tiziana Bonaldi , Michael F. Seidl , Jérȏme Collemare","doi":"10.1016/j.fgb.2023.103800","DOIUrl":"10.1016/j.fgb.2023.103800","url":null,"abstract":"<div>In eukaryotes, the combination of different histone post-translational modifications (PTMs) – the histone code – impacts the chromatin organization as compact and transcriptionally silent heterochromatin or accessible and transcriptionally active euchromatin. Although specific histone PTMs have been studied in fungi, an overview of histone PTMs and their relative abundance is still lacking. Here, we used mass spectrometry to detect and quantify histone PTMs in three fungal species belonging to three distinct taxonomic sections of the genus Aspergillus (Aspergillus niger, Aspergillus nidulans (two strains), and Aspergillus fumigatus). We overall detected 23 different histone PTMs, including a majority of lysine methylations and acetylations, and 23 co-occurrence patterns of multiple histone PTMs. Among those, we report for the first time the detection of H3K79me1, H3K79me2, and H4K31ac in Aspergilli. Although all three species harbour the same PTMs, we found significant differences in the relative abundance of H3K9me1/2/3, H3K14ac, H3K36me1 and H3K79me1, as well as the co-occurrence of acetylation on both K18 and K23 of histone H3 in a strain-specific manner. Our results provide novel insights about the underexplored complexity of the histone code in filamentous fungi, and its functional implications on genome architecture and gene regulation.</div>","PeriodicalId":55135,"journal":{"name":"Fungal Genetics and Biology","volume":"167 ","pages":"Article 103800"},"PeriodicalIF":3.0,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9704397","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The C2H2-type zinc-finger regulator AoKap5 is required for the growth and kojic acid synthesis in Aspergillus oryzae c2h2型锌指调节剂AoKap5是米曲霉生长和合成曲酸所必需的

IF 3 3区生物学

Fungal Genetics and Biology Pub Date : 2023-06-01 DOI: 10.1016/j.fgb.2023.103813

Yuzhen Li , Ziming Chen , Feng Zhang , Tianming Chen , Junxia Fan , Xin Deng , Xiaocui Lei , Bin Zeng , Zhe Zhang

{"title":"The C2H2-type zinc-finger regulator AoKap5 is required for the growth and kojic acid synthesis in Aspergillus oryzae","authors":"Yuzhen Li , Ziming Chen , Feng Zhang , Tianming Chen , Junxia Fan , Xin Deng , Xiaocui Lei , Bin Zeng , Zhe Zhang","doi":"10.1016/j.fgb.2023.103813","DOIUrl":"https://doi.org/10.1016/j.fgb.2023.103813","url":null,"abstract":"<div>Aspergillus oryzae is an important filamentous fungus widely used for the industrial production of fermented foods and secondary metabolites. The clarifying of the mechanism of the growth and secondary metabolites in A. oryzae is important for its industrial production and utilization. Here, the C2H2-type zinc-finger protein AoKap5 was characterized to be involved in the growth and kojic acid production in A. oryzae. The Aokap5-disrupted mutants were constructed by the CRISPR/Cas9 system, which displayed increased colony growth but decreased conidial formation. Deletion of Aokap5 enhanced the tolerance to cell-wall and oxidative but not osmotic stress. The transcriptional activation assay revealed that AoKap5 itself didn’t have transcriptional activation activity. Disruption of Aokap5 resulted in the reduced production of kojic acid, coupled with the reduced expression of the kojic acid synthesis genes kojA and kojT. Meanwhile, overexpression of kojT could rescue the decreased production of kojic acid in Aokap5-deletion strain, indicating that Aokap5 serves upstream of kojT. Furthermore, the yeast one-hybrid assay demonstrated that AoKap5 could directly bind to the kojT promoter. These findings suggest that AoKap5 regulates kojic acid production through binding to the kojT promoter. This study provides an insight into the role of zinc finger protein in the growth and kojic acid biosynthesis of A. oryzae.</div>","PeriodicalId":55135,"journal":{"name":"Fungal Genetics and Biology","volume":"167 ","pages":"Article 103813"},"PeriodicalIF":3.0,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49860062","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Whole genome resequencing reveal patterns of genetic variation within Colletotrichum acutatum species complex from rubber trees in China 全基因组重测序揭示了中国橡胶树炭疽菌(Colletotrichum actuatum)种复合体的遗传变异模式

IF 3 3区生物学

Fungal Genetics and Biology Pub Date : 2023-06-01 DOI: 10.1016/j.fgb.2023.103801

Xianbao Liu, Boxun Li, Jimiao Cai, Tao Shi, Yang Yang, Yanli Feng, Guixiu Huang

{"title":"Whole genome resequencing reveal patterns of genetic variation within Colletotrichum acutatum species complex from rubber trees in China","authors":"Xianbao Liu, Boxun Li, Jimiao Cai, Tao Shi, Yang Yang, Yanli Feng, Guixiu Huang","doi":"10.1016/j.fgb.2023.103801","DOIUrl":"10.1016/j.fgb.2023.103801","url":null,"abstract":"<div>The Colletotrichum acutatum species complex possesses a diverse number of important traits, such as a wide host range and host preference, different modes of reproduction, and different strategies of host infection. Research using comparative genomics has attempted to find correlations between these traits. Here, we used multi-locus techniques and gene genealogical concordance analysis to investigate the phylogenetic relationships and taxonomic status of the Colletotrichum acutatum species complex using field isolates obtained from rubber trees. The results revealed that the dominant species was C. australisinense, followed by C. bannaense, while strain YNJH17109 was identified as C. laticiphilum. The taxonomic status of strains YNLC510 and YNLC511 was undetermined. Using whole-genome single nucleotide polymorphism data to analyze population structure, 18 strains of C. australisinense were subsequently divided into four populations, one of which was derived from an admixture of two populations. In addition, the strains LD1687, GD1628, and YNLC516, did not belong to any populations, and were considered to be admixtures of two or more populations. A split decomposition network analysis also provided evidence for genetic recombination within Colletotrichum acutatum species complex from rubber trees in China. Overall, a weak phylogeographic sub-structure was observed. Analysis also revealed significant differences in morphological characters and levels of virulence between populations.</div>","PeriodicalId":55135,"journal":{"name":"Fungal Genetics and Biology","volume":"167 ","pages":"Article 103801"},"PeriodicalIF":3.0,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9650633","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Redox perturbations in yeast cells lacking glutathione reductase 缺乏谷胱甘肽还原酶的酵母细胞中的氧化还原扰动

IF 3 3区生物学

Fungal Genetics and Biology Pub Date : 2023-06-01 DOI: 10.1016/j.fgb.2023.103810

Agnieszka Janeczko , Michał Przywara , Roman Maslanka, Barbara Raś, Klaudia Ziaja, Magdalena Kwolek-Mirek, Renata Zadrag-Tecza, Sabina Bednarska

{"title":"Redox perturbations in yeast cells lacking glutathione reductase","authors":"Agnieszka Janeczko , Michał Przywara , Roman Maslanka, Barbara Raś, Klaudia Ziaja, Magdalena Kwolek-Mirek, Renata Zadrag-Tecza, Sabina Bednarska","doi":"10.1016/j.fgb.2023.103810","DOIUrl":"10.1016/j.fgb.2023.103810","url":null,"abstract":"<div>Cellular redox homeostasis has a major effect on cell functions and its maintenance is supported by glutathione and protein thiols which serve as redox buffers in cells. The regulation of the glutathione biosynthetic pathway is a focus of a lot of scientific research. However, still little is known about how complex cellular networks influence glutathione homeostasis. In this work was used an experimental system based on an S. cerevisiae yeast mutant with a lack of the glutathione reductase enzyme and allyl alcohol as a precursor of acrolein inside the cell to determine the cellular processes influencing glutathione homeostasis. The absence of Glr1p slows down the growth rate of the cell population, especially in the presence of allyl alcohol, but does not lead to complete inhibition of the cell's reproductive capacity. It also amends the GSH/GSSG ratio and the share of NADPH and NADP+ in the total NADP(H) pool. The obtained results show that potential pathways involved in the maintenance of redox homeostasis are based from one side on de novo synthesis of GSH as indicated by increased activity of γ-GCS and increased expression of GSH1 gene in the Δglr1 mutant, from the other hand, on increased the level of NADPH. This is because the lower ratio of GSH/GSSG can be counterbalanced with the NADPH/NADP+ alternative system. The higher level of NADPH can be used by the thioredoxin system and other enzymes requiring NADPH to reduce cytosolic GSSG and maintain glutathione redox potential.</div>","PeriodicalId":55135,"journal":{"name":"Fungal Genetics and Biology","volume":"167 ","pages":"Article 103810"},"PeriodicalIF":3.0,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9651300","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparative proteomic analysis of two divergent strains provides insights into thermotolerance mechanisms of Ganoderma lingzhi 两个不同菌株的比较蛋白质组学分析为灵芝的耐热机制提供了新的思路

IF 3 3区生物学

Fungal Genetics and Biology Pub Date : 2023-06-01 DOI: 10.1016/j.fgb.2023.103796

Manjun Cai , Xiaoxian Wu , Xiaowei Liang , Huiping Hu , Yuanchao Liu , Tianqiao Yong , Xiangmin Li , Chun Xiao , Xiong Gao , Shaodan Chen , Yizhen Xie , Qingping Wu

{"title":"Comparative proteomic analysis of two divergent strains provides insights into thermotolerance mechanisms of Ganoderma lingzhi","authors":"Manjun Cai , Xiaoxian Wu , Xiaowei Liang , Huiping Hu , Yuanchao Liu , Tianqiao Yong , Xiangmin Li , Chun Xiao , Xiong Gao , Shaodan Chen , Yizhen Xie , Qingping Wu","doi":"10.1016/j.fgb.2023.103796","DOIUrl":"10.1016/j.fgb.2023.103796","url":null,"abstract":"<div>Heat stress (HS) is a major abiotic factor influencing fungal growth and metabolism. However, the genetic basis of thermotolerance in Ganoderma lingzhi (G. lingzhi) remains largely unknown. In this study, we investigated the thermotolerance capacities of 21 G. lingzhi strains and screened the thermo-tolerant (S566) and heat-sensitive (Z381) strains. The mycelia of S566 and Z381 were collected and subjected to a tandem mass tag (TMT)-based proteome assay. We identified 1493 differentially expressed proteins (DEPs), with 376 and 395 DEPs specific to the heat-tolerant and heat-susceptible genotypes, respectively. In the heat-tolerant genotype, upregulated proteins were linked to stimulus regulation and response. Proteins related to oxidative phosphorylation, glycosylphosphatidylinositol-anchor biosynthesis, and cell wall macromolecule metabolism were downregulated in susceptible genotypes. After HS, the mycelial growth of the heat-sensitive Z381 strain was inhibited, and mitochondrial cristae and cell wall integrity of this strain were severely impaired, suggesting that HS may inhibit mycelial growth of Z381 by damaging the cell wall and mitochondrial structure. Furthermore, thermotolerance-related regulatory pathways were explored by analyzing the protein-protein interaction network of DEPs considered to participate in the controlling the thermotolerance capacity. This study provides insights into G. lingzhi thermotolerance mechanisms and a basis for breeding a thermotolerant germplasm bank for G. lingzhi and other fungi.</div>","PeriodicalId":55135,"journal":{"name":"Fungal Genetics and Biology","volume":"167 ","pages":"Article 103796"},"PeriodicalIF":3.0,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9704398","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The evolution and ecology of psilocybin in nature 裸盖菇素在自然界中的进化与生态学

IF 3 3区生物学

Fungal Genetics and Biology Pub Date : 2023-06-01 DOI: 10.1016/j.fgb.2023.103812

Matthew Meyer , Jason Slot

引用次数: 0

A transcription factor and a phosphatase regulate temperature-dependent morphogenesis in the fungal plant pathogen Zymoseptoria tritici 一种转录因子和一种磷酸酶调节真菌植物病原菌酵母的温度依赖性形态发生

IF 3 3区生物学

Fungal Genetics and Biology Pub Date : 2023-06-01 DOI: 10.1016/j.fgb.2023.103811

Carolina Sardinha Francisco, Bruce A. McDonald, Javier Palma-Guerrero

{"title":"A transcription factor and a phosphatase regulate temperature-dependent morphogenesis in the fungal plant pathogen Zymoseptoria tritici","authors":"Carolina Sardinha Francisco, Bruce A. McDonald, Javier Palma-Guerrero","doi":"10.1016/j.fgb.2023.103811","DOIUrl":"10.1016/j.fgb.2023.103811","url":null,"abstract":"<div>Naturally fluctuating temperatures provide a constant environmental stress that requires adaptation. Some fungal pathogens respond to heat stress by producing new morphotypes that maximize their overall fitness. The fungal wheat pathogen Zymoseptoria tritici responds to heat stress by switching from its yeast-like blastospore form to hyphae or chlamydospores. The regulatory mechanisms underlying this switch are unknown. Here, we demonstrate that a differential heat stress response is ubiquitous in Z. tritici populations around the world. We used QTL mapping to identify a single locus associated with the temperature-dependent morphogenesis and we found two genes, the transcription factor ZtMsr1 and the protein phosphatase ZtYvh1, regulating this mechanism. We find that ZtMsr1 regulates repression of hyphal growth and induces chlamydospore formation whereas ZtYvh1 is required for hyphal growth. We next showed that chlamydospore formation is a response to the intracellular osmotic stress generated by the heat stress. This intracellular stress stimulates the cell wall integrity (CWI) and high-osmolarity glycerol (HOG) MAPK pathways resulting in hyphal growth. If cell wall integrity is compromised, however, ZtMsr1 represses the hyphal development program and may induce the chlamydospore-inducing genes as a stress-response survival strategy. Taken together, these results suggest a novel mechanism through which morphological transitions are orchestrated in Z. tritici – a mechanism that may also be present in other pleomorphic fungi.</div>","PeriodicalId":55135,"journal":{"name":"Fungal Genetics and Biology","volume":"167 ","pages":"Article 103811"},"PeriodicalIF":3.0,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9655057","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2