Hereditas最新文献_第5页

Effect on the expression of drd2 and drd3 after neonatal lesion in the lymphocytes, nucleus accumbens, hippocampus and prefrontal cortex: comparative analysis between juvenile and adult Wistar rats 新生儿损伤后淋巴细胞、伏隔核、海马和前额皮质中drd2和drd3表达的影响:幼鼠与成年Wistar大鼠的比较分析

IF 2.7 3区生物学

Hereditas Pub Date : 2016-11-22 DOI: 10.1186/s41065-016-0018-9

A. Genis-Mendoza, C. Tovilla-Zárate, Lilia López-Narváez, P. Mendoza-Lorenzo, P. Ostrosky-Wegman, H. Nicolini, T. González-Castro, Yazmín Hernández-Díaz

引用次数: 5

Efficient callus formation and plant regeneration are heritable characters in sugar beet (Beta vulgaris L.) 甜菜(Beta vulgaris L.)愈伤组织形成和植株再生的遗传特性

IF 2.7 3区生物学

Hereditas Pub Date : 2016-11-15 DOI: 10.1186/s41065-016-0015-z

Hiroyo Kagami, K. Taguchi, Takumi Arakawa, Y. Kuroda, H. Tamagake, T. Kubo

引用次数: 7

Whole genome SNP typing to investigate methicillin-resistant Staphylococcus aureus carriage in a health-care provider as the source of multiple surgical site infections 全基因组SNP分型调查医疗保健提供者中耐甲氧西林金黄色葡萄球菌携带作为多个手术部位感染的来源

IF 2.7 3区生物学

Hereditas Pub Date : 2016-11-14 DOI: 10.1186/s41065-016-0017-x

Chandler C. Roe, Kim Horn, E. Driebe, J. Bowers, J. Terriquez, P. Keim, D. Engelthaler

引用次数: 10

Genetic diversity and occurrence of the F129L substitutions among isolates of Alternaria solani in south-eastern Sweden. 瑞典东南部Alternaria solani分离物的遗传多样性和F129L置换的发生。

IF 2.7 3区生物学

Hereditas Pub Date : 2016-09-23 eCollection Date: 2016-01-01 DOI: 10.1186/s41065-016-0014-0

Firuz Odilbekov, Eva Edin, Larisa Garkava-Gustavsson, Helena Persson Hovmalm, Erland Liljeroth

{"title":"Genetic diversity and occurrence of the F129L substitutions among isolates of Alternaria solani in south-eastern Sweden.","authors":"Firuz Odilbekov, Eva Edin, Larisa Garkava-Gustavsson, Helena Persson Hovmalm, Erland Liljeroth","doi":"10.1186/s41065-016-0014-0","DOIUrl":"10.1186/s41065-016-0014-0","url":null,"abstract":"Background: Early blight, caused by the fungus Alternaria solani, occurs on potato mainly in the south-eastern part of Sweden, but also in other parts of the country. The aim of this study was to investigate the genetic diversity of A. solani populations from different potato growing regions in south-eastern Sweden using AFLP marker analysis. In addition, the cultured isolates were examined for substitutions in the gene encoding cytochrome b, associated with loss of sensitivity against QoI fungicides.Results: Nei's gene diversity index for the Swedish populations of A. solani revealed a gene diversity of up to 0.20. Also genetic differentiation was observed among populations of A. solani from different locations in south-eastern Sweden. The mitochondrial genotype of the isolates of A. solani was determined and both known genotypes, GI (genotype 1) and GII (genotype 2), were found among the isolates. The occurrence of the F129L substitution associated with a loss of sensitivity to strobilurins was confirmed among the GII isolates. In vitro conidial germination tests verified that isolates containing the F129L substitution had reduced sensitivity to azoxystrobin and, at a lower extent, to pyraclostrobin.Conclusions: Genetic diversity was relatively high among isolates of A. solani in south-eastern part of Sweden. F129L substitutions, leading to reduced sensitivity to strobilurins, have been established in field populations, which may have implications for the future efficacy of QoI fungicides.","PeriodicalId":55057,"journal":{"name":"Hereditas","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2016-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5226105/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"65774505","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Increasing the efficiency of homologous recombination vector-mediated end joining repair by inhibition of Lig4 gene using siRNA in sheep embryo fibroblasts. 利用siRNA抑制Lig4基因提高羊胚胎成纤维细胞同源重组载体介导的末端连接修复效率。

IF 2.7 3区生物学

Hereditas Pub Date : 2016-09-01 DOI: 10.16288/j.yczz.16-074

Wei Wang, Yu-shuang Wang, Lan-lan Huang, Zi-jian Jian, Xin-Hua Wang, Shou-ren Liu, Wen-hui Pi

{"title":"Increasing the efficiency of homologous recombination vector-mediated end joining repair by inhibition of Lig4 gene using siRNA in sheep embryo fibroblasts.","authors":"Wei Wang, Yu-shuang Wang, Lan-lan Huang, Zi-jian Jian, Xin-Hua Wang, Shou-ren Liu, Wen-hui Pi","doi":"10.16288/j.yczz.16-074","DOIUrl":"https://doi.org/10.16288/j.yczz.16-074","url":null,"abstract":"In animal cells, inhibition of non-homologous end joining (NHEJ) pathway improves the efficiency of homologous recombination (HR)-mediated double-strand brakes (DSBs) repair. To improve the efficiency of HR in sheep embryo fibroblasts, the NHEJ key molecule DNA ligase 4 (Lig4) was suppressed by siRNA interference. Four pairs of siRNA targeting Lig4 were designed and chemically synthesized. These siRNA were electro-transferred into sheep embryo fibroblasts respectively. Compared with the control groups, two pairs of siRNA were identified to effectively inhibit the expression of sheep Lig4 gene by qRT-PCR and Western blotting. The plasmid rejoining assay was adopted for examining the efficiency of HR-mediated DSB repair. I-SceⅠ endonuclease linearized vector and siRNA were co-transfected into sheep embryo fibroblasts. Flow cytometry analysis of cells after transfection for 72 h showed that suppression of Lig4 using siRNAs increased the rejoining efficiency of HR vector by 3-4 times compared with the control groups. Therefore, enhanced HR vector rejoining frequency by instant inhabition of Lig4 gene provides theoretical basis for improving gene targeting efficiency of sheep embryo fibroblasts.","PeriodicalId":55057,"journal":{"name":"Hereditas","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2016-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67512674","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Research advances on microbial genetics in China in 2015. 2015年中国微生物遗传学研究进展。

IF 2.7 3区生物学

Hereditas Pub Date : 2016-09-01 DOI: 10.16288/J.YCZZ.16-305

X. Jianping, H. Yubo, L. Gang, B. Linquan

{"title":"Research advances on microbial genetics in China in 2015.","authors":"X. Jianping, H. Yubo, L. Gang, B. Linquan","doi":"10.16288/J.YCZZ.16-305","DOIUrl":"https://doi.org/10.16288/J.YCZZ.16-305","url":null,"abstract":"In 2015, there are significant progresses in many aspects of the microbial genetics in China. To showcase the contribution of Chinese scientists in microbial genetics, this review surveys several notable progresses in microbial genetics made largely by Chinese scientists, and some key findings are highlighted. For the basic microbial genetics, the components, structures and functions of many macromolecule complexes involved in gene expression regulation have been elucidated. Moreover, the molecular basis underlying the recognition of foreign nucleic acids by microbial immune systems was unveiled. We also illustrated the biosynthetic pathways and regulators of multiple microbial compounds, novel enzyme reactions, and new mechanisms regulating microbial gene expression. And new findings were obtained in the microbial development, evolution and population genetics. For the industrial microbiology, more understanding on the molecular basis of the microbial factory has been gained. For the pathogenic microbiology, the genetic circuits of several pathogens were depicted, and significant progresses were achieved for understanding the pathogen-host interaction and revealing the genetic mechanisms underlying antimicrobial resistance, emerging pathogens and environmental microorganisms at the genomic level. In future, the genetic diversity of microbes can be used to obtain specific products, while gut microbiome is gathering momentum.","PeriodicalId":55057,"journal":{"name":"Hereditas","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2016-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.16288/J.YCZZ.16-305","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67512879","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Insight into the genetic variability analysis and cultivar identification of tall fescue by using SSR markers 利用SSR标记分析高羊茅的遗传变异及品种鉴定

IF 2.7 3区生物学

Hereditas Pub Date : 2016-08-11 DOI: 10.1186/s41065-016-0013-1

Kaixin Fu, Zhihui Guo, Xinquan Zhang, Yan Fan, Wendan Wu, Daxu Li, Yan Peng, Linkai Huang, Ming-Mao Sun, S. Bai, Xiao Ma

引用次数: 12

Genetic structure of Mount Huang honey bee (Apis cerana) populations: evidence from microsatellite polymorphism 黄山蜜蜂群体遗传结构:来自微卫星多态性的证据

IF 2.7 3区生物学

Hereditas Pub Date : 2016-07-06 DOI: 10.1186/s41065-016-0010-4

Fang Liu, Tengfei Shi, Sisi Huang, Linsheng Yu, S. Bi

引用次数: 10

Assessment of diversity and genetic relationships of Neonectria ditissima: the causal agent of fruit tree canker 果树溃疡病病原新树蝇的多样性及亲缘关系评价

IF 2.7 3区生物学

Hereditas Pub Date : 2016-07-01 DOI: 10.1186/s41065-016-0011-3

M. Ghasemkhani, L. Garkava-Gustavsson, E. Liljeroth, H. Nybom

引用次数: 9

eRFSVM: a hybrid classifier to predict enhancers-integrating random forests with support vector machines. eRFSVM：预测增强子的混合分类器--集成随机森林与支持向量机。

IF 2.7 3区生物学

Hereditas Pub Date : 2016-06-30 eCollection Date: 2016-01-01 DOI: 10.1186/s41065-016-0012-2

Fang Huang, Jiawei Shen, Qingli Guo, Yongyong Shi

{"title":"eRFSVM: a hybrid classifier to predict enhancers-integrating random forests with support vector machines.","authors":"Fang Huang, Jiawei Shen, Qingli Guo, Yongyong Shi","doi":"10.1186/s41065-016-0012-2","DOIUrl":"10.1186/s41065-016-0012-2","url":null,"abstract":"Background: Enhancers are tissue specific distal regulation elements, playing vital roles in gene regulation and expression. The prediction and identification of enhancers are important but challenging issues for bioinformatics studies. Existing computational methods, mostly single classifiers, can only predict the transcriptional coactivator EP300 based enhancers and show low generalization performance.Results: We built a hybrid classifier called eRFSVM in this study, using random forests as a base classifier, and support vector machines as a main classifier. eRFSVM integrated two components as eRFSVM-ENCODE and eRFSVM-FANTOM5 with diverse features and labels. The base classifier trained datasets from a single tissue or cell with random forests. The main classifier made the final decision by support vector machines algorithm, with the predicting results of base classifiers as inputs. For eRFSVM-ENCODE, we trained datasets from cell lines including Gm12878, Hep, H1-hesc and Huvec, using ChIP-Seq datasets as features and EP300 based enhancers as labels. We tested eRFSVM-ENCODE on K562 dataset, and resulted in a predicting precision of 83.69 %, which was much better than existing classifiers. For eRFSVM-FANTOM5, with enhancers identified by RNA in FANTOM5 project as labels, the precision, recall, F-score and accuracy were 86.17 %, 36.06 %, 50.84 % and 93.38 % using eRFSVM, increasing 23.24 % (69.92 %), 97.05 % (18.30 %), 76.90 % (28.74 %), 4.69 % (89.20 %) than the existing algorithm, respectively.Conclusions: All these results demonstrated that eRFSVM was a better classifier in predicting both EP300 based and FAMTOM5 RNAs based enhancers.","PeriodicalId":55057,"journal":{"name":"Hereditas","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2016-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5226099/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"65774437","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0