Genetic diversity and occurrence of the F129L substitutions among isolates of Alternaria solani in south-eastern Sweden.

IF 2.1 3区生物学 Q3 GENETICS & HEREDITY

Hereditas Pub Date : 2016-09-23 eCollection Date: 2016-01-01 DOI:10.1186/s41065-016-0014-0

Firuz Odilbekov, Eva Edin, Larisa Garkava-Gustavsson, Helena Persson Hovmalm, Erland Liljeroth

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引用次数: 0

Abstract

Background: Early blight, caused by the fungus Alternaria solani, occurs on potato mainly in the south-eastern part of Sweden, but also in other parts of the country. The aim of this study was to investigate the genetic diversity of A. solani populations from different potato growing regions in south-eastern Sweden using AFLP marker analysis. In addition, the cultured isolates were examined for substitutions in the gene encoding cytochrome b, associated with loss of sensitivity against QoI fungicides.

Results: Nei's gene diversity index for the Swedish populations of A. solani revealed a gene diversity of up to 0.20. Also genetic differentiation was observed among populations of A. solani from different locations in south-eastern Sweden. The mitochondrial genotype of the isolates of A. solani was determined and both known genotypes, GI (genotype 1) and GII (genotype 2), were found among the isolates. The occurrence of the F129L substitution associated with a loss of sensitivity to strobilurins was confirmed among the GII isolates. In vitro conidial germination tests verified that isolates containing the F129L substitution had reduced sensitivity to azoxystrobin and, at a lower extent, to pyraclostrobin.

Conclusions: Genetic diversity was relatively high among isolates of A. solani in south-eastern part of Sweden. F129L substitutions, leading to reduced sensitivity to strobilurins, have been established in field populations, which may have implications for the future efficacy of QoI fungicides.

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瑞典东南部Alternaria solani分离物的遗传多样性和F129L置换的发生。

背景：由Alternaria solani真菌引起的马铃薯早疫病主要发生在瑞典的东南部地区，但也发生在瑞典的其他地区。本研究的目的是利用 AFLP 标记分析法研究来自瑞典东南部不同马铃薯种植区的 A. solani 群体的遗传多样性。此外，还检测了培养分离物中编码细胞色素 b 的基因是否发生了替代，这与对 QoI 杀菌剂丧失敏感性有关：瑞典种群的内氏基因多样性指数（Nei's gene diversity index）显示基因多样性高达 0.20。瑞典东南部不同地区的 A. solani 种群之间也存在基因差异。对 A. solani 分离物的线粒体基因型进行了测定，在分离物中发现了两种已知的基因型：GI（基因型 1）和 GII（基因型 2）。在 GII 型分离物中证实了 F129L 替代的发生，它对石硫合剂失去了敏感性。体外分生孢子萌发试验证实，含有 F129L 替代的分离株对唑菌酰胺的敏感性降低，对吡唑醚菌酯的敏感性也有所降低：结论：瑞典东南部的茄属真菌分离物的遗传多样性相对较高。在田间种群中已经发现了 F129L 替代，这种替代会导致对石硫合剂的敏感性降低，这可能会对 QoI 杀菌剂的未来药效产生影响。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Hereditas 生物-遗传学

CiteScore

4.30

自引率

3.70%

发文量

审稿时长

6 weeks

期刊介绍： For almost a century, Hereditas has published original cutting-edge research and reviews. As the Official journal of the Mendelian Society of Lund, the journal welcomes research from across all areas of genetics and genomics. Topics of interest include human and medical genetics, animal and plant genetics, microbial genetics, agriculture and bioinformatics.