Human Immunology最新文献

Possible significance of anti-HLA DQ antibodies after lung transplantation – Single centre study 肺移植后抗hla - DQ抗体的可能意义-单中心研究

IF 2.2 4区医学

Human Immunology Pub Date : 2025-10-10 DOI: 10.1016/j.humimm.2025.111597

Anna Dukat-Mazurek , Wojtek Karolak , Hanna Zielińska , Patrycja Specjał , Grażyna Moszkowska , Jacek Wojarski , Karolina Lipka , Justyna Fercho , Marta Gallas , Dariusz Rystwiej , Fanny Sunesson , Lin Akily , William Karlsen , Marcin Sawczuk , Piotr Trzonkowski , Sławomir Żegleń

{"title":"Possible significance of anti-HLA DQ antibodies after lung transplantation – Single centre study","authors":"Anna Dukat-Mazurek , Wojtek Karolak , Hanna Zielińska , Patrycja Specjał , Grażyna Moszkowska , Jacek Wojarski , Karolina Lipka , Justyna Fercho , Marta Gallas , Dariusz Rystwiej , Fanny Sunesson , Lin Akily , William Karlsen , Marcin Sawczuk , Piotr Trzonkowski , Sławomir Żegleń","doi":"10.1016/j.humimm.2025.111597","DOIUrl":"10.1016/j.humimm.2025.111597","url":null,"abstract":"<div><div>The role of anti-HLA antibodies in solid organ transplantation has long been recognized, particularly with respect to graft rejection and patient survival. This study aimed to evaluate the impact of <em>de novo</em> donor-specific antibodies (DSA) on graft failure and mortality in Polish lung transplant recipients. The study included 112 patients who had a well-defined immune status prior to transplantation. The presence and specificity of anti-HLA antibodies were assessed using the Luminex platform. We identified 26 patients (23%) as having increased immunological risk. Pre-transplant DSAs were detected in 13 recipients. Post-transplantation, the development of <em>de novo</em> DSAs was evaluated, with a cumulative incidence of 11%. Our findings confirmed that lung transplant recipients with donor-specific immunization frequently develop antibodies targeting antigens at the HLA-DQ locus. Notably, 90% (9 out of 10) of immunized patients exhibited high mean fluorescence intensity (MFI) levels of anti-DQ antibodies. These antibodies were shown to be harmful, with mortality significantly higher in immunized patients: 50% of patients with anti-DQ antibodies died, compared to only 15% in those without DSAs. This is the first study of its kind in the Polish population, confirming that strategies to prevent and treat DQ DSAs may improve outcomes for lung transplant recipients.</div></div>","PeriodicalId":55047,"journal":{"name":"Human Immunology","volume":"86 6","pages":"Article 111597"},"PeriodicalIF":2.2,"publicationDate":"2025-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145269610","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

miR-302d-3p expression and genotype are associated with CMV replication after allogeneic haematopoietic stem cell transplantation miR-302d-3p的表达和基因型与同种异体造血干细胞移植后巨细胞病毒的复制有关。

IF 2.2 4区医学

Human Immunology Pub Date : 2025-10-08 DOI: 10.1016/j.humimm.2025.111599

Piotr Łacina , Rachel E Crossland , Jagoda Siemaszko , Agnieszka Szeremet , Maciej Majcherek , Anna Czyż , Małgorzata Sobczyk-Kruszelnicka , Wojciech Fidyk , Iwona Solarska , Barbara Nasiłowska-Adamska , Patrycja Skowrońska , Maria Bieniaszewska , Agnieszka Tomaszewska , Grzegorz W Basak , Sebastian Giebel , Tomasz Wróbel , Katarzyna Bogunia-Kubik

{"title":"miR-302d-3p expression and genotype are associated with CMV replication after allogeneic haematopoietic stem cell transplantation","authors":"Piotr Łacina , Rachel E Crossland , Jagoda Siemaszko , Agnieszka Szeremet , Maciej Majcherek , Anna Czyż , Małgorzata Sobczyk-Kruszelnicka , Wojciech Fidyk , Iwona Solarska , Barbara Nasiłowska-Adamska , Patrycja Skowrońska , Maria Bieniaszewska , Agnieszka Tomaszewska , Grzegorz W Basak , Sebastian Giebel , Tomasz Wróbel , Katarzyna Bogunia-Kubik","doi":"10.1016/j.humimm.2025.111599","DOIUrl":"10.1016/j.humimm.2025.111599","url":null,"abstract":"<div><div>Cytomegalovirus (CMV) reactivation is a common complication after allogeneic haematopoietic stem cell transplantation (HSCT). It occurs in over a third of alloHSCT recipients, and is a major source of post-transplant mortality. miRNAs are small non-coding RNA molecules that are important regulators of gene expression. They are often dysregulated during disease, including transplant-related complications. Due to their presence in all body fluids, they can be used as potential biomarkers. In this study, we performed miRNA profiling to find differentially expressed host miRNAs in serum that could be linked to CMV reactivation after HSCT. CMV reactivation was confirmed by detection of CMV DNA in serum. We identified two miRNAs, miR-302d-3p (p < 0.001) and miR-6721-5p (p = 0.018), as differentially expressed between day + 30 and + 90 after HSCT in patients with CMV reactivation. Of these, miR-302d-3p was confirmed as overexpressed on day + 30 in a verification analysis performed on an independent cohort of patients (p = 0.027). Furthermore, we analysed two genetic variants in the gene coding for miR-302d-3p. We found one of them, rs13136737, to be significantly associated with CMV reactivation (p = 0.015). In conclusion, our study showed that miR-302d-3p is dysregulated and that its genetic variant rs13136737 may be important in development of CMV reactivation after HSCT.</div></div>","PeriodicalId":55047,"journal":{"name":"Human Immunology","volume":"86 6","pages":"Article 111599"},"PeriodicalIF":2.2,"publicationDate":"2025-10-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145260208","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

One Action and Multiple Results: Influenza A Virus and SARS-CoV-2 Poly-Epitope-Based Hybrid Vaccine Exploration by Reverse Vaccinology and Immunoinformatic Methods 一个作用和多个结果：基于反向疫苗学和免疫信息学方法的甲型流感病毒和SARS-CoV-2多表位杂交疫苗的探索

IF 2.2 4区医学

Human Immunology Pub Date : 2025-10-04 DOI: 10.1016/j.humimm.2025.111588

Fatemeh Razmjooei , Mohammad Izadi , Abbas Dehghanian , Sina Hassani , Hamed Mir , Mirza Ali Mofazzal Jahromi , Mohammad Aref Bagherzadeh

{"title":"One Action and Multiple Results: Influenza A Virus and SARS-CoV-2 Poly-Epitope-Based Hybrid Vaccine Exploration by Reverse Vaccinology and Immunoinformatic Methods","authors":"Fatemeh Razmjooei , Mohammad Izadi , Abbas Dehghanian , Sina Hassani , Hamed Mir , Mirza Ali Mofazzal Jahromi , Mohammad Aref Bagherzadeh","doi":"10.1016/j.humimm.2025.111588","DOIUrl":"10.1016/j.humimm.2025.111588","url":null,"abstract":"<div><h3>Introduction</h3><div>Influenza A virus (IAV) and severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) coinfection have been shown to intensify symptoms and, therefore, could be considered a significant threat to susceptible populations. Although vaccination is an effective and safe strategy to be used against viruses, large amounts of mutations in these two viruses remain an issue for vaccine design, as some strains of the IAV and SARS-CoV-2 are still present worldwide, regardless of the introduction of various vaccines to protect against the viruses.</div></div><div><h3>Methods</h3><div>We employed a bioinformatic approach to design a hybrid vaccine based on the epitopes of IAV and SARS-CoV-2, which are potentially conserved against mutations and are safe in terms of toxicity and allergenicity, yet promise to induce long-lasting innate and adaptive immune responses.</div></div><div><h3>Results</h3><div>The selected peptides demonstrated a potentially effective immune response and showed promise as safe and suitable for hybrid vaccine design. We selected epitopes focusing on their binding quality to cytotoxic T lymphocyte (CTL), helper T lymphocyte (HTL), and B cell. For instance, CTL′s epitopes were checked using sequence-based prediction methods, and their entropy and antigenicity were confirmed. Similarly, all epitopes were selected based on their ability to induce robust humoral and cell-mediated immunity, respectively.</div></div><div><h3>Conclusion</h3><div>Hybrid vaccine design, utilizing our approach, can lead to next-generation hybrid vaccines that help the healthcare system establish a more consistent defense against both viruses for the susceptible population.</div></div>","PeriodicalId":55047,"journal":{"name":"Human Immunology","volume":"86 6","pages":"Article 111588"},"PeriodicalIF":2.2,"publicationDate":"2025-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145223186","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Does hydroxychloroquine intervention improve pregnancy outcomes in patients with recurrent pregnancy loss who are positive for antinuclear antibodies? 羟氯喹干预是否能改善抗核抗体阳性的复发性流产患者的妊娠结局？

IF 2.2 4区医学

Human Immunology Pub Date : 2025-09-22 DOI: 10.1016/j.humimm.2025.111585

Li Yang , Yueqiu Shen , Minqin Xu , Ling Zhou , Hong Zhang

{"title":"Does hydroxychloroquine intervention improve pregnancy outcomes in patients with recurrent pregnancy loss who are positive for antinuclear antibodies?","authors":"Li Yang , Yueqiu Shen , Minqin Xu , Ling Zhou , Hong Zhang","doi":"10.1016/j.humimm.2025.111585","DOIUrl":"10.1016/j.humimm.2025.111585","url":null,"abstract":"<div><div>Whether hydroxychloroquine intervention can improve pregnancy outcomes in patients with recurrent pregnancy loss patients (RPL) with antinuclear antibodies (ANAs) positive is still unknown.</div><div>RPL patients who were positive for ANAs without rheumatism were recruited from the Reproductive Medicine Center of the Second Affiliated Hospital of Soochow University from January 2016 to December 2023 (N = 104). Normal healthy pregnant women were selected as the Control group (N = 100). ANA-positive pregnant women who had experienced one sporadic abortion were included in the SA group (N = 42). Patients in both RPL and SA groups received 0.2–0.4 g hydroxychloroquine daily. General data and pregnancy outcomes were compared among the three groups. Patients with RPL were divided into three subgroups according to ANA titers: 1:100 (n = 81), 1:320 (n = 18) and 1:1000 (n = 5). The differences in the pregnancy outcomes among the subgroups were compared.</div><div>We found the abortion rates in RPL and SA groups were greater than that in the control group (<em>P</em> < 0.05). The incidence of pregnancy complications was greater in the RPL group than in the control group (<em>P</em> < 0.05). The full-term live birth rates in RPL and SA groups were lower than that in the control group (<em>P</em> < 0.05). There were significant differences in the abortion rate among patients with RPL with different ANA titers (<em>P</em> < 0.05).</div><div>We concluded that adverse pregnancy outcomes, such as abortion and complications during pregnancy, are still more common in ANA-positive RPL patients than in normal pregnant women despite treatment. The ANA titer is closely related to the abortion rate among RPL patients during sequent pregnancy.</div></div>","PeriodicalId":55047,"journal":{"name":"Human Immunology","volume":"86 6","pages":"Article 111585"},"PeriodicalIF":2.2,"publicationDate":"2025-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145108650","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dual liver-heart transplantation for highly allosensitized heart failure patients 高度异体化心力衰竭患者的双肝心脏移植

IF 2.2 4区医学

Human Immunology Pub Date : 2025-09-22 DOI: 10.1016/j.humimm.2025.111587

Shin Lin , Elaine Chou-Wu , Mark Sturdevant , Yiing Lin , Jay Pal , Idoia Gimferrer

引用次数: 0

Innate alloimmunity: SIRPA mismatches between the donor and recipient are associated with kidney allograft outcome 先天性异体免疫：供体和受体之间的SIRPA不匹配与肾移植的结果有关

IF 2.2 4区医学

Human Immunology Pub Date : 2025-09-01 DOI: 10.1016/j.humimm.2025.111354

A. Senev , A. Tambur , D. Metes , F. Lakkis , A. Cherukuri

引用次数: 0

A noninvasive urine biomarker CXCL10 bead-based detection assay for monitoring renal transplants 一种无创尿液生物标志物CXCL10头部检测方法用于监测肾脏移植