European Food Research and Technology最新文献

{"title":"Application of metabolomics and chemometrics for authentication of vegetable oils and adulteration detection: a review","authors":"Najwa Sulaiman,&nbsp;Maimunah Sanny,&nbsp;Chee Beng Yeoh,&nbsp;Azmil Haizam Ahmad Tarmizi,&nbsp;Noordiana Nordin,&nbsp;Farah Khuwailah Ahmad Bustamam,&nbsp;Raznim Arni Abd Razak,&nbsp;Ahmad Faizal Abdull Razis,&nbsp;Alfi Khatib","doi":"10.1007/s00217-025-04832-9","DOIUrl":"10.1007/s00217-025-04832-9","url":null,"abstract":"<div><p>The authentication and detection of adulteration in food are critical for ensuring food safety, quality and consumer protection. Vegetable oils are one of the significant diets in human nutrition, and hence its authenticity as well as safety are of utmost importance to ensure safe consumption and fairness of international trade. Metabolomics coupled with chemometrics, have been widely used as an accurate measurement of complex metabolite profiles in vegetable oils. This integrated methodology allows for the full identification of chemical compositions of vegetable oils and the detection of potential adulterants. This review outlines recent advances and applications of metabolomics in combination with chemometrics for the authentication and adulteration detection in vegetable oils. Various chromatographic and spectroscopic techniques combined with chemometrics such as multivariate statistical analysis for interpretation of big data sets enables the discovery of potential characteristic markers for authentication and adulteration detection in vegetable oils. Identifying the most suitable analytical technique and chemometrics tool helps in the marker discovery for authentication purposes, and can be further explored for the development of rapid detection technique for safety assessment by authorized regulatory bodies.</p></div>","PeriodicalId":549,"journal":{"name":"European Food Research and Technology","volume":"251 10","pages":"3175 - 3198"},"PeriodicalIF":3.2,"publicationDate":"2025-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145227907","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Discordance between in silico and in vitro results on proteolytic stability of milk protein-derived DPP-4 inhibitory peptides","authors":"Amila Turalić,&nbsp;Zsófia Hegedüs,&nbsp;Fabio Altieri,&nbsp;Tamás A. Martinek,&nbsp;Jasmina Đeđibegović","doi":"10.1007/s00217-025-04833-8","DOIUrl":"10.1007/s00217-025-04833-8","url":null,"abstract":"<div><p>The resistance of food-derived bioactive peptides to gastrointestinal enzymatic degradation remains a key challenge to address before considering their in vivo activity. While many in vitro studies explored peptide activity, proteolytic stability studies are limited. The proteolytic stability of some of the most potent proline-containing, milk protein-derived DPP-4 inhibitory peptides (IPI, IPA, IPM, LPQNIPPL, and INNQFLPYPY) from the BIOPEP-UWM database was initially screened using BIOPEP-UWM, ExPASy PeptideCutter, and ProsperousPlus in silico webservers. The peptides were synthesized and incubated separately in vitro with pepsin, chymotrypsin, or pancreatic elastase. The peptides were proteolytically stable during 3 h incubation, except INNQFLPYPY, which was partially degraded by chymotrypsin, supporting previous findings on hydrolysis resistance. However, the cleavage sites predicted by in silico tools were not fully confirmed by in vitro experiment. Misclassified cleavage sites were observed for proline-, asparagine-, or glutamine-containing peptide bonds, highlighting potential limitations of the commonly used in silico tools. To assess the observed differences, a statistical analysis was performed to evaluate predictive accuracy using a validation dataset of 897 previously tested cleavage sites. The in silico and in vitro agreement on cleavage sites was below 75%. Negative predictive values, supported by specificity data, indicate that in silico tools may miss 10–30% of the cleavage sites. The obtained likelihood ratio values (LR+ &lt; 10, LR- &gt; 0.1) did not meet the expected standards of accuracy. Analysis of the underlying methodology of the in silico tools clarified the potential causes of conflicting results and identified areas for improvement.</p></div>","PeriodicalId":549,"journal":{"name":"European Food Research and Technology","volume":"251 10","pages":"3199 - 3214"},"PeriodicalIF":3.2,"publicationDate":"2025-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145227894","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A comparison of bovine and porcine species in foods containing animal gelatin with RT-PCR and Q-Exactive ORBITRAP device","authors":"Nuray Gamze Yörük","doi":"10.1007/s00217-025-04836-5","DOIUrl":"10.1007/s00217-025-04836-5","url":null,"abstract":"<p>In this research, the performances of Real Time Polymerase Chain Reaction (RT-PCR) and Q-Exactive Orbitrap (Orbitrap MS) techniques were compared. The performance of both methods in identifying bovine and porcine gelatin components in sample categories such as chocolate, candy, biscuits, food supplements, jelly beans, etc. was considered. Results showed that Orbitrap MS achieved a high detection rate of 98.75% (100% for beef and 97.5% for pork), particularly in complex matrices. In contrast, RT-PCR showed acceptable performance only in simpler food matrices, with clear limitations in detecting degraded DNA in processed products. Overall, the results confirm the notion that Orbitrap molecular techniques should be preferred where precision and reliability are of utmost importance, such as regulatory compliance, especially halal certification.</p>","PeriodicalId":549,"journal":{"name":"European Food Research and Technology","volume":"251 10","pages":"3215 - 3224"},"PeriodicalIF":3.2,"publicationDate":"2025-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145227895","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nepetanudoside B, a potential new food additive isolated from Nepeta aristata: antioxidant, antibacterial, DFT, ADME/T, PASS prediction and MTT analysis studies","authors":"Yunus Basar,&nbsp;Semiha Yenigun,&nbsp;Tevfik Ozen,&nbsp;Ibrahim Demirtas,&nbsp;Ozge Balpınar,&nbsp;Lutfi Behcet","doi":"10.1007/s00217-025-04831-w","DOIUrl":"10.1007/s00217-025-04831-w","url":null,"abstract":"<div><p>Nutritional problems and food shortages worldwide drive increased focus on research on new and highly effective food supplements. Nepetanudoside B (NNB), an iridoid derivative found in <i>Nepeta</i> species, was isolated by <i>preparative</i> HPLC from the active methanol fraction of <i>Nepeta aristata</i>. The results showed that NNB was more effective than α-tocopherol in reducing power and phosphomolybdenum reducing activities. On the other hand, NNB was more effective than ascorbic acid in H₂O₂ and superoxide anion radical scavenging activities. NNB showed effective antimicrobial activity against <i>S. aureus</i>,<i> E. faecalis</i>, and <i>K. pneumoniae</i> pathogens. Moreover, the NNB has high gastrointestinal absorption and low toxicity in ADME/T prediction. In the DFT study, NNB had low softness (0.181 eV^-1), high hardness (2.762 eV), substantial electrophilicity value (2.179 eV), and BDE value (108.79 kcal/mol). The PASS was utilized to forecast the biological activity profiles of NNB before biological testing. In the MTT test, it was observed how different doses of NNB in the human oral fibroblast cell line affected cell viability, and it was found that NNB had no harmful effects. Therefore, NNB can be used in the food industry due to its antioxidant and antibacterial effects.</p><h3>Graphical Abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":549,"journal":{"name":"European Food Research and Technology","volume":"251 10","pages":"3123 - 3139"},"PeriodicalIF":3.2,"publicationDate":"2025-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s00217-025-04831-w.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145227951","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Flavonoids from aging tangerine peel alleviate OVA-induced allergic asthma through JAK/STAT signaling pathway in balb/c mice","authors":"Xiaoming Sun,&nbsp;Kunyi Liu,&nbsp;Le yu,&nbsp;Baojun Shan,&nbsp;Leixi Ye,&nbsp;Yunqi Shan,&nbsp;Hongjie Xu,&nbsp;Quan Yang","doi":"10.1007/s00217-025-04820-z","DOIUrl":"10.1007/s00217-025-04820-z","url":null,"abstract":"<div><p>The dry pericarp of the fruit of <i>Citrus reticulata</i> Blanco cultivar ‘Chachi’ is known as Guang ChenPi (GCP) in Guangdong province in China, which should be stored for more than three years for optimal bioactivity, especially for spleen-strengthening and cough relief. However, the molecular mechanisms and the impact of aging years on bioactivity in cough relief remain poorly understood. Herein, we investigate the efficacy of GCP of different aging years using an allergic asthma Balb/c mouse model in which airway hyperreactivity was induced by ovalbumin (OVA). The results showed that aqueous extracts of GCP of different aging years could reduce lung inflammation and airway tissue lesion, and could inhibit the expression of tracheal MUC5AC protein, IL-4, IL-5, and IL-13 mRNA levels in lung tissue and bronchoalveolar lavage fluid (BALF) through the JAK/STAT pathway. Furthermore, we have identified 11 constituents absorbed in blood based on UHPLC-Orbitrap MS/MS and metabolism results showed that linoleic acid metabolism and sphingolipid signaling pathways are the primary mechanisms of the effort. The Grey correlation degree indicated the top three constituents of tangeretin, quinic acid and 2’,5,6-trimethoxyflavone, which were closely related to the effects of drying dampness and phlegm elimination. Importantly, our findings underscored that GCP of different aging years showed good efficacy in rescuing IL-13 and IL-4 levels in serum and BALF. This study provides new evidence that flavonoids in aging GCP could modulate activity towards alleviating OVA-induced allergic asthma through the JAK/STAT pathway.</p></div>","PeriodicalId":549,"journal":{"name":"European Food Research and Technology","volume":"251 10","pages":"3071 - 3086"},"PeriodicalIF":3.2,"publicationDate":"2025-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145227889","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of biochemical profiles in walnut (Juglans regia L.) genotypes and predictive modeling using advanced machine learning algorithms","authors":"Fatih Temizyürek,&nbsp;Muhammet Ali Gündeşli,&nbsp;Yavuz Canbay,&nbsp;Hacı Osman Özatar,&nbsp;Esra Gölcü,&nbsp;Vahdet Cemil Altun,&nbsp;Ömer Atagül,&nbsp;Murat Güney,&nbsp;Ebru Kafkas,&nbsp;Remzi Ugur,&nbsp;Mehmet Sütyemez","doi":"10.1007/s00217-025-04826-7","DOIUrl":"10.1007/s00217-025-04826-7","url":null,"abstract":"<div><p>Biochemical parameters such as phenolic compounds, tocopherols and polyunsaturated fatty acids (omega-6 and omega-3 etc.) found in walnuts support cardiovascular health with their high antioxidant content, reduce inflammation and improve the blood lipid profile and show positive effects such as lowering LDL cholesterol, increasing HDL and reducing oxidative stress. This study investigates the application of machine learning algorithms to predict the biochemical properties of 10 walnut (<i>Juglans regia</i> L.) genotypes selected from the Kahramanmaraş region and three commonly cultivated walnut cultivars (Maraş-18, Chandler, and Franquette). Plant material was collected between 2020–2021, and analyses were conducted on biochemical components, including polyphenols, antioxidant capacity, protein, oil, fatty acids, sugars, and organic acids using HPLC, GC, and spectrophotometric methods. The highest total phenolic content was found in genotype 46KM-5 (402.6 mg GAE/100 g), and the highest antioxidant capacity in genotype 46KM-13 (73.77%). The 46KM-9 genotype exhibited the highest oil content (71.60%) and the highest total sugar content (3.27%), while sucrose was the dominant sugar. Regarding fatty acids, genotype 46KM-19 had the highest linoleic acid content (60.88%), and genotype 46KM-21 showed the highest oleic acid content (20.88%). Malic acid emerged as the dominant organic acid, with the highest content observed in genotype 46KM-9 (6.55%). In addition, various machine learning algorithms such as such as decision tree, support vector regression, K-nearest neighbor (KNN), gradient boosting, multi-layer perceptron, and linear regression (LR) have been used to assess the biochemical properties of genotypes using hyperparameter optimization. KNN algorithm generally provided high performance in prediction processes by exhibiting very low error rates. This study's results align with previous research, highlighting walnut genotype variation and nutritional benefits. Machine learning predicts key traits, aiding breeders and consumers in health-focused production and better genotype selection for improved cultivation.</p></div>","PeriodicalId":549,"journal":{"name":"European Food Research and Technology","volume":"251 10","pages":"3105 - 3122"},"PeriodicalIF":3.2,"publicationDate":"2025-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s00217-025-04826-7.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145227950","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigation of antidiabetic, hypolipidemic and hypocholesterolemic effects of antioxidant-enriched microcrystalline cellulose","authors":"Naciye Ünver","doi":"10.1007/s00217-025-04834-7","DOIUrl":"10.1007/s00217-025-04834-7","url":null,"abstract":"<div><p>A large amount of almond shells and hulls remain as agro-industrial waste in almond processing. Since these wastes are generally not utilized, they cause environmental pollution. However, the almond hull is rich in phenolic compounds, whereas the almond shell is rich in lignocellulosic compounds. In this study, the antidiabetic, hypolipidemic and hypocholesterolemic effects of a complex of phenolic extract and microcrystalline cellulose (antioxidant-enriched microcrystalline cellulose) produced from almond hull and shell were investigated. Firstly, inhibitory activities on α-amylase and α-glucosidase were examined to determine the antidiabetic activity of phenolic extract obtained from almond hull (PE-AH), microcrystalline cellulose obtained from almond shell (MCC-AS) and antioxidant-enriched microcrystalline cellulose (AE-MCC-AS). In these examinations, it was found that PE-AH (IC₅₀:11.24 for α-amylase and IC₅₀:10.95 for α- glucosidase) had the highest activity against both enzymes, followed by AE-MCC-AS (IC₅₀: 14.10 for α-amylase and IC₅₀: 15.77 for α- glucosidase) and MCC-AS (IC₅₀:51.37 for α-amylase and IC₅₀: 28.99 for α- glucosidase), respectively. It was determined that MCC-AS and AE-MCC-AS showed glucose absorption even at low glucose concentrations and had similar effects in terms of glucose diffusion. Similar to the antidiabetic activity, PE-AH had the highest activity on lipase (IC₅₀: 3.64), followed by AE-MCC-AS (IC₅₀: 13.90) and MCC-AS (IC₅₀: 71.92), respectively. Regarding cholesterol adsorption capacity, AE-MCC-AS had approximately 8-fold more adsorption capacity than MCC-AS, indicating that phenolic compounds were more effective than MCC on cholesterol adsorption capacity. In conclusion, the study results demonstrated that AE-MCC-AS is a versatile MCC-phenolic extract complex that exhibits antidiabetic, hypolipidemic and hypocholesterolemic effects.</p></div>","PeriodicalId":549,"journal":{"name":"European Food Research and Technology","volume":"251 10","pages":"3141 - 3150"},"PeriodicalIF":3.2,"publicationDate":"2025-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s00217-025-04834-7.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145227952","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Using fluorescence spectra to quantitatively evaluate the constituent content and functionality of hydroponically grown Fragaria × Ananassa","authors":"Hidemichi Sano,&nbsp;Satoru Kawaguchi,&nbsp;Toshifumi Iimori,&nbsp;Masahiro Kuragano,&nbsp;Kiyotaka Tokuraku,&nbsp;Koji Uwai","doi":"10.1007/s00217-025-04821-y","DOIUrl":"10.1007/s00217-025-04821-y","url":null,"abstract":"<div><p>Strawberry leaves contain polyphenolic compounds such as elagitannins and flavonoids and are thought to possess antioxidant and amyloid-β (Aβ) aggregation inhibitory properties. However, strawberry leaves are usually discarded because their functional components vary with growth stages and environmental factors, and a method that immediately evaluates such functional strawberry leaf components in the field has not yet been developed. In this study, the fluorescence spectra obtained by irradiating strawberry leaves with excitation light were used to analyze strawberry leaves during hydroponic cultivation. Standard methods that assess the functional components (chlorophyll, total polyphenol and flavonoid content, ellagic acid, ferulic acid, naringenin) and functionality (free radical–scavenging activity, ferric reducing antioxidant power, oxygen radical absorptivity, and Aβ aggregation inhibition activity) of plants were performed. The correlations between fluorescence spectra and these parameters were analyzed using spectral indices analyses. The correlation between each spectral index and component amount and functionality of the obtained predictive model was 0.84 ≥ <i>R</i> ≥ 0.61. This new method facilitates the simultaneous and nondestructive prediction of both component content and functionality of strawberry leaves and allows the determination of optimal strawberry leaves for utilization as a source of functional foods and medicinal material.</p></div>","PeriodicalId":549,"journal":{"name":"European Food Research and Technology","volume":"251 10","pages":"3087 - 3103"},"PeriodicalIF":3.2,"publicationDate":"2025-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145227906","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring recent advances in carrageenan-based films for sustainable food packaging applications","authors":"Thulasidhas Dhilipkumar,&nbsp;Abdellatif M. Sadeq,&nbsp;P Karuppusamy,&nbsp;Karthik V. Shankar,&nbsp;Arun Prasad Murali,&nbsp;Abdulbasit A. Darem,&nbsp;Karuppaiah Selvakumar,&nbsp;Jayant Giri","doi":"10.1007/s00217-025-04835-6","DOIUrl":"10.1007/s00217-025-04835-6","url":null,"abstract":"<div><p>The use of non-renewable plastic packaging poses significant environmental challenges. To address these issues, researchers are focused on developing sustainable packaging materials that are environmentally friendly alternatives. The carrageenan (CG), a polysaccharide derived from seaweed, has been processed into a packaging film and studied for food packaging applications due to its distinctive physicochemical properties. CG-based films have garnered attention due to their abundant availability, ability to form films, and overall effectiveness. The current review outlines recent advances in the preparation of CG-based films for various fields of food packaging, such as active, edible, and antimicrobial packaging. Moreover, a brief insight is provided into the effect of additive inclusion on physicochemical, antimicrobial, and antioxidant properties of CG-based films. The suitability of different types of CG, such as kappa (κ), iota (ι), and lambda (λ) for food packaging applications is also discussed by considering their individual film-forming abilities, gel strength, and compatibility with functional additives. The use of CG-based films in food preservation, including their ability to extend the shelf life of fruits, vegetables, meats, and dairy products, is also reviewed. Furthermore, the review addresses the challenges and limitations associated with CG-based films, such as their sensitivity to moisture, mechanical strength, and difficulties in commercial-scale production. The consumer perception and safety aspects related to the use of CG films in food contact applications are also highlighted in the present review. Overall, this review presents a comprehensive overview of CG-based films, covering their preparation, properties, applications, safety, limitations, and future research directions for their development as eco-friendly food packaging materials.</p></div>","PeriodicalId":549,"journal":{"name":"European Food Research and Technology","volume":"251 10","pages":"3151 - 3173"},"PeriodicalIF":3.2,"publicationDate":"2025-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s00217-025-04835-6.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145227953","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Volatile flavor characteristics and microbial fermentation differences of water kefir grains from different origins","authors":"Yani Wang,&nbsp;Xiaolei Wang,&nbsp;Yonghui Xu,&nbsp;Yanlong Li,&nbsp;Yunguo Liu,&nbsp;Xiangna Lin","doi":"10.1007/s00217-025-04823-w","DOIUrl":"10.1007/s00217-025-04823-w","url":null,"abstract":"<div><p>To assess the quality differences of water kefir (WK) grains from different regions, this study investigated the volatile flavor components and microbial composition of sugar-containing water kefir fermented beverages. Understanding these variations is crucial for optimizing fermentation conditions and developing starter cultures with distinct flavor profiles. The volatile components of water kefir beverages from eight regions were analyzed using an electronic nose (E-nose) and headspace solid-phase microextraction gas chromatography-mass spectrometry (HS-SPME-GC-MS). Partial least squares discriminant analysis (PLS-DA) effectively differentiated the flavor profiles of samples from different regions. GC-MS analysis identified 41 volatile compounds, eight of which were key differential compounds, including isoamyl acetate, ethyl octanoate, ethyl 4-hydroxymandelate, ethyl decanoate, Propan-1-ol, ethanol, acetic acid, and octanoic acid. High-throughput sequencing and correlation analysis revealed that specific microbial taxa, such as <i>Pediococcus</i>, <i>Lactobacillaceae</i>, and <i>Novosphingobium resinovorum</i>, were closely associated with the production of these key volatile compounds. Based on the obtained data, this study provides new insights into the relationship between microbial composition and volatile compounds during water kefir fermentation, which will facilitate the development of water kefir products with consistent and desirable flavor characteristics.</p></div>","PeriodicalId":549,"journal":{"name":"European Food Research and Technology","volume":"251 10","pages":"3019 - 3032"},"PeriodicalIF":3.2,"publicationDate":"2025-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145227905","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}