{"title":"A comparison of bovine and porcine species in foods containing animal gelatin with RT-PCR and Q-Exactive ORBITRAP device","authors":"Nuray Gamze Yörük","doi":"10.1007/s00217-025-04836-5","DOIUrl":null,"url":null,"abstract":"<p>In this research, the performances of Real Time Polymerase Chain Reaction (RT-PCR) and Q-Exactive Orbitrap (Orbitrap MS) techniques were compared. The performance of both methods in identifying bovine and porcine gelatin components in sample categories such as chocolate, candy, biscuits, food supplements, jelly beans, etc. was considered. Results showed that Orbitrap MS achieved a high detection rate of 98.75% (100% for beef and 97.5% for pork), particularly in complex matrices. In contrast, RT-PCR showed acceptable performance only in simpler food matrices, with clear limitations in detecting degraded DNA in processed products. Overall, the results confirm the notion that Orbitrap molecular techniques should be preferred where precision and reliability are of utmost importance, such as regulatory compliance, especially halal certification.</p>","PeriodicalId":549,"journal":{"name":"European Food Research and Technology","volume":"251 10","pages":"3215 - 3224"},"PeriodicalIF":3.2000,"publicationDate":"2025-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"European Food Research and Technology","FirstCategoryId":"97","ListUrlMain":"https://link.springer.com/article/10.1007/s00217-025-04836-5","RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"FOOD SCIENCE & TECHNOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
In this research, the performances of Real Time Polymerase Chain Reaction (RT-PCR) and Q-Exactive Orbitrap (Orbitrap MS) techniques were compared. The performance of both methods in identifying bovine and porcine gelatin components in sample categories such as chocolate, candy, biscuits, food supplements, jelly beans, etc. was considered. Results showed that Orbitrap MS achieved a high detection rate of 98.75% (100% for beef and 97.5% for pork), particularly in complex matrices. In contrast, RT-PCR showed acceptable performance only in simpler food matrices, with clear limitations in detecting degraded DNA in processed products. Overall, the results confirm the notion that Orbitrap molecular techniques should be preferred where precision and reliability are of utmost importance, such as regulatory compliance, especially halal certification.
期刊介绍：
The journal European Food Research and Technology publishes state-of-the-art research papers and review articles on fundamental and applied food research. The journal''s mission is the fast publication of high quality papers on front-line research, newest techniques and on developing trends in the following sections:
-chemistry and biochemistry-
technology and molecular biotechnology-
nutritional chemistry and toxicology-
analytical and sensory methodologies-
food physics.
Out of the scope of the journal are:
- contributions which are not of international interest or do not have a substantial impact on food sciences,
- submissions which comprise merely data collections, based on the use of routine analytical or bacteriological methods,
- contributions reporting biological or functional effects without profound chemical and/or physical structure characterization of the compound(s) under research.
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
