Iranian Journal of Immunology最新文献_第9页

rMBP-NAP Suppresses OXA-induced Allergic Dermatitis by Regulating the Th1/Th2 Balance. rMBP-NAP通过调节Th1/Th2平衡抑制oxa诱导的变应性皮炎。

IF 0.9 4区医学

Iranian Journal of Immunology Pub Date : 2023-03-14 DOI: 10.22034/iji.2023.92594.2157

Hao Zheng, Qiaozhen Kang, Chenglong Zhang, Lu Yang, Xin Liu

{"title":"rMBP-NAP Suppresses OXA-induced Allergic Dermatitis by Regulating the Th1/Th2 Balance.","authors":"Hao Zheng, Qiaozhen Kang, Chenglong Zhang, Lu Yang, Xin Liu","doi":"10.22034/iji.2023.92594.2157","DOIUrl":"https://doi.org/10.22034/iji.2023.92594.2157","url":null,"abstract":"Background: Allergic dermatitis (AD) is an inflammatory skin disease that arises from abnormal T lymphocyte activation. A recombinant fusion protein comprising Helicobacter pylori neutrophil-activating protein and maltose binding protein, rMBP-NAP, has been documented as a novel immunomodulatory TLR agonist.Objective: To explore the effect of the rMBP-NAP on the OXA-induced AD in a mouse model and clarify the possible action mechanism.Methods: The AD animal model was induced by repeated administration of oxazolone (OXA) in BALB/c mice. H&E staining was used to analyze the ear epidermis thickness and the number of infiltrating inflammatory cells. TB staining was used to detect mast cell infiltration in the ear tissue. ELISA was used to analyze the secretion of cytokines IL-4 and IFN-γ in peripheral blood. qRT-PCR was used to determine the expression levels of IL-4, IFN-γ, and IL-13 in ear tissue.Results: OXA induced the establishment of an AD model. After the rMBP-NAP treatment, the thickness of the ear tissue and the number of mast cells infiltrated in AD mice reduced, and the serum and ear tissue levels of IL-4 and IFN-γ increased, but the ratio of IFN-γ (rMBP-NAP group)/IL-4 (rMBP-NAP group) was greater than the ratio of IFN-γ (sensitized group)/IL-4 (sensitized group).Conclusion: The rMBP-NAP improved the disease symptoms including skin lesions in AD, alleviated the inflammation in ear tissue, and restored the Th1/2 balance by inducing a shift from the Th2 to the Th1 response. The results of our work support the use of rMBP-NAP as an immunomodulator for AD treatment in future investigations.","PeriodicalId":54921,"journal":{"name":"Iranian Journal of Immunology","volume":"20 1","pages":"36-44"},"PeriodicalIF":0.9,"publicationDate":"2023-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9239064","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Methyl-cpg-binding Domain Protein 2 Silencing Inhibits Th17 Differentiation of CD4+T cells Induced by Ovalbumin. 甲基-cpg结合区域蛋白2沉默抑制卵白蛋白诱导CD4+T细胞的Th17分化

IF 0.9 4区医学

Iranian Journal of Immunology Pub Date : 2023-03-14 DOI: 10.22034/iji.2023.93312.2212

Yan Jiang, Linqiao Li, Qilu Pan, Xiaojing Du, Qian Han, Feixiang Ling, Rou Li, Lin Mai, Jianwei Huang, Shuyuan Chu, Libing Ma

{"title":"Methyl-cpg-binding Domain Protein 2 Silencing Inhibits Th17 Differentiation of CD4+T cells Induced by Ovalbumin.","authors":"Yan Jiang, Linqiao Li, Qilu Pan, Xiaojing Du, Qian Han, Feixiang Ling, Rou Li, Lin Mai, Jianwei Huang, Shuyuan Chu, Libing Ma","doi":"10.22034/iji.2023.93312.2212","DOIUrl":"https://doi.org/10.22034/iji.2023.93312.2212","url":null,"abstract":"Background: Little is known about MBD2's epigenetic regulation in the immune pathogenesis of CD4+T cell differentiation.Objective: This study attempted to explore the mechanism of methyl-cpg-binding domain protein 2 (MBD2) in CD4+T cell differentiation stimulated by environmental allergen ovalbumin (OVA).Methods: Mononuclear cells were separated from the spleen tissues of male C57BL/6 mice. The OVA interfered with the differentiation of splenic mononuclear cells and CD4+T cells. The CD4+T cells were obtained by magnetic beads and identified by CD4 labeled antibody. CD4+T cells were transfected with lentivirus to silence MBD2 gene. A methylation quantification kit was used to detect 5-mC levels.Results: The purity of CD4+T cells reached 95.99% after magnetic beads sorting. Treatment with 200 μg/mL OVA stimulated the CD4+T cells differentiation to Th17 cells and promoted the secretion of IL-17. After being induced, the Th17 cell ratio increased. 5-Aza inhibited the Th17 cell differentiation and the IL-17 level in a dose-dependent manner. Under the intervention of the Th17 induction and 5-Aza, MBD2 silencing inhibited the differentiation of Th17 cell, and decreased the IL-17 and 5-mC levels in the cell supernatants. MBD2 silencing reduced the scale of the Th17 cell and IL-17 levels in the OVA-treated CD4+T cells.Conclusion: MBD2 affected IL-17 and 5-mC levels by mediating the Th17 cell differentiation in splenic CD4+T cells that were interfered with 5-Aza. OVA induced Th17 differentiation and increased IL-17 levels, inhibited by MBD2 silencing.","PeriodicalId":54921,"journal":{"name":"Iranian Journal of Immunology","volume":"20 1","pages":"45-56"},"PeriodicalIF":0.9,"publicationDate":"2023-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9239065","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Vaccine Adjuvants: Selection Criteria, Mechanism of Action Associated with Immune Responses and Future Directions. 疫苗佐剂:选择标准、与免疫应答相关的作用机制和未来方向。

IF 0.9 4区医学

Iranian Journal of Immunology Pub Date : 2023-03-14 DOI: 10.22034/iji.2023.94097.2284

Arslan Habib, Khalid Mahmood Anjum, Riffat Iqbal, Ghulam Jaffar, Zeeshan Ashraf, Malik ShahZaib Khalid, Muhammad Usman Taj, Syeda Wafa Zainab, Muhammad Umair, Muhammad Zohaib, Tabinda Khalid

{"title":"Vaccine Adjuvants: Selection Criteria, Mechanism of Action Associated with Immune Responses and Future Directions.","authors":"Arslan Habib, Khalid Mahmood Anjum, Riffat Iqbal, Ghulam Jaffar, Zeeshan Ashraf, Malik ShahZaib Khalid, Muhammad Usman Taj, Syeda Wafa Zainab, Muhammad Umair, Muhammad Zohaib, Tabinda Khalid","doi":"10.22034/iji.2023.94097.2284","DOIUrl":"https://doi.org/10.22034/iji.2023.94097.2284","url":null,"abstract":"The most effective method to minimize the prevalence of infectious diseases is vaccination. Vaccines enhance immunity and provide protection against different kinds of infections. Subunit vaccines are safe and less toxic, but due to their lower immunogenicity, they need adjuvants to boost the immune system. Adjuvants are small particles/molecules integrated into a vaccine to enhance the immunogenic feedback of antigens. They play a significant role to enhance the potency and efficiency of vaccines. There are several types of adjuvants with different mechanisms of action; therefore, improved knowledge of their immunogenicity will help develop a new generation of adjuvants. Many trials have been designed using different kinds of vaccine adjuvants to examine their safety and efficacy, but in practice, only a few have entered in animal and human clinical trials. However, for the development of safe and effective vaccines, it is important to have adequate knowledge of the side effects and toxicity of different adjuvants. The current review discussed the adjuvants which are available for producing modern vaccines as well as some new classes of adjuvants in clinical trials.","PeriodicalId":54921,"journal":{"name":"Iranian Journal of Immunology","volume":"20 1","pages":"1-15"},"PeriodicalIF":0.9,"publicationDate":"2023-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9134120","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Methylation Profile of Cancer Testis Antigens in Colorectal Cancer. 结直肠癌中癌睾丸抗原的甲基化谱。

IF 0.9 4区医学

Iranian Journal of Immunology Pub Date : 2023-03-14 DOI: 10.22034/iji.2023.92600.2171

Nadiah Abu, Nurul Ainaa Adilah Rus Bakarurraini, Siti Nurmi Nasir, Muhiddin Ishak, Rashidah Baharuddin, Rahman Jamal, Nurul Syakima Ab Mutalib

{"title":"Methylation Profile of Cancer Testis Antigens in Colorectal Cancer.","authors":"Nadiah Abu, Nurul Ainaa Adilah Rus Bakarurraini, Siti Nurmi Nasir, Muhiddin Ishak, Rashidah Baharuddin, Rahman Jamal, Nurul Syakima Ab Mutalib","doi":"10.22034/iji.2023.92600.2171","DOIUrl":"https://doi.org/10.22034/iji.2023.92600.2171","url":null,"abstract":"Background: Cancer testis antigens (CTAs) are a class of immune-stimulating antigens often overexpressed in many types of cancers. The usage of the CTAs as immunotherapy targets have been widely investigated in different cancers including melanoma, hematological malignancies, and colorectal cancer. Studies have indicated that the epigenetic regulation of the CTAs such as the methylation status may affect the expression of the CTAs. However, the report on the methylation status of the CTAs is conflicting. The general methylation profile of the CTAs, especially in colorectal cancer, is still elusive.Objective: To determine the methylation profile of the selected CTAs in our colorectal cancer patients.Methods: A total of 54 pairs of colorectal cancer samples were subjected to DNA methylation profiling using the Infinium Human Methylation 450K bead chip.Results: We found that most of the CTAs were hypomethylated, and CCNA1 and TMEM108 genes were among the few CTAs that were hypermethylated.Conclusion: Overall, our brief report has managed to show the overall methylation profile in over the 200 CTAs in colorectal cancer and this could be used for further refining any immunotherapy targets.","PeriodicalId":54921,"journal":{"name":"Iranian Journal of Immunology","volume":"20 1","pages":"83-91"},"PeriodicalIF":0.9,"publicationDate":"2023-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9232956","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Activation of β2-adrenergic Receptor Alleviates Collagen-induced Arthritis by Ameliorating Th17/Treg Imbalance. β2-肾上腺素能受体的激活通过改善Th17/Treg失衡缓解胶原诱导关节炎。

IF 0.9 4区医学

Iranian Journal of Immunology Pub Date : 2023-03-14 DOI: 10.22034/iji.2023.94125.2286

Jian-Hua Lu, Xiao-Xiao Rui, Ting-Ting Wang, Xiao-Qin Wang, Yu-Ping Peng, Yi-Hua Qiu

{"title":"Activation of β2-adrenergic Receptor Alleviates Collagen-induced Arthritis by Ameliorating Th17/Treg Imbalance.","authors":"Jian-Hua Lu, Xiao-Xiao Rui, Ting-Ting Wang, Xiao-Qin Wang, Yu-Ping Peng, Yi-Hua Qiu","doi":"10.22034/iji.2023.94125.2286","DOIUrl":"https://doi.org/10.22034/iji.2023.94125.2286","url":null,"abstract":"Background: Recent research in our laboratory shows that CD4+ T cells express the β2 adrenergic receptor (β2-AR), and the sympathetic neurotransmitter norepinephrine regulates the function of T cells via β2-AR signaling. However, the immunoregulatory effect of β2-AR and its related mechanisms on rheumatoid arthritis is unknown.Objective: To explore the effects of β2-AR in collagen-induced arthritis (CIA) on the imbalance of T helper (Th) 17/ regulatory T (Treg) cells.Methods: In DBA1/J mice, collagen type II was injected intradermally at the tail base to prepare the CIA model. The specific β2-AR agonist, terbutaline (TBL), was administered intraperitoneally beginning on day 31 and continuing until day 47 after primary vaccination, twice a day. Magnetic beads were used to sort CD3+ T cells subsets from spleen tissues.Results: In vivo, β2-AR agonist TBL alleviated arthritis symptoms in the CIA mice including histopathology of the ankle joints, four limbs' arthritis score, the thickness of ankle joints, and rear paws. After TBL treatment, in the ankle joints, the levels of proinflammatory factors (IL-17/22) notably decreased and the levels of immunosuppressive factors (IL-10/TGF-β) significantly increased. In vitro, ROR-γt protein expression, Th17 cell number, mRNA expression and the releasing of IL-17/22 from CD3+ T cells reduced following TBL administration. Moreover, TBL enhanced the anti-inflammatory responses of Treg cells.Conclusion: These results suggest that β2-AR activation exerts anti-inflammatory effects through the amelioration of Th17/Treg imbalance in the CIA disease.","PeriodicalId":54921,"journal":{"name":"Iranian Journal of Immunology","volume":"20 1","pages":"16-25"},"PeriodicalIF":0.9,"publicationDate":"2023-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9197206","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Variations in Macrophage Activation Syndrome-associated Cardiac Diseases: A Report on Two Cases. 巨噬细胞激活综合征相关心脏病的变异:两例报告

IF 0.9 4区医学

Iranian Journal of Immunology Pub Date : 2023-03-14 DOI: 10.22034/iji.2023.93355.2218

Na Lin, Liping Xu, Qiaoding Dai

引用次数: 0

The Evaluation of the Effect of Tolerogenic Probiotics on the Maturation of Healthy Dendritic Cells versus Immature Dendritic Cells. 耐受性益生菌对健康树突状细胞与未成熟树突状细胞成熟影响的评价。

IF 0.9 4区医学

Iranian Journal of Immunology Pub Date : 2023-03-14 DOI: 10.22034/iji.2023.91783.2131

Maryam Rastin, Mahmoud Mahmoudi, Nafiseh Tabasi, Nadia Kia, Jafar Hajavi, Seyed-Alireza Esmaeili

{"title":"The Evaluation of the Effect of Tolerogenic Probiotics on the Maturation of Healthy Dendritic Cells versus Immature Dendritic Cells.","authors":"Maryam Rastin, Mahmoud Mahmoudi, Nafiseh Tabasi, Nadia Kia, Jafar Hajavi, Seyed-Alireza Esmaeili","doi":"10.22034/iji.2023.91783.2131","DOIUrl":"https://doi.org/10.22034/iji.2023.91783.2131","url":null,"abstract":"Background: Dendritic cells, (DCs) as one of the important immune cell populations, are responsible for the initiation, development, and control of acquired immune responses. Myeloid dendritic cells can be used as a vaccine for several autoimmune diseases and cancers. Tolerogenic probiotics with regulatory properties can affect the maturation and development of immature dendritic cells (IDC) into mature DCs with certain immunomodulatory effects.Objective: To assess the immunomodulatory effect of Lactobacillus rhamnosus and Lactobacillus delbrueckii, as two tolerogenic probiotics, in the differentiation and maturation of myeloid dendritic cells.Methods: The IDCs were derived from the healthy donors in GM-CSF and IL 4 medium. Mature DCs (MDC) were produced with L. delbrueckii, L. rhamnosus, and LPS from IDCs. Real-Time PCR and flow cytometry were used to confirm the DC maturation and to determine DC markers as well as IDO, IL10, and IL12 expression levels, respectively.Results: Probiotic-derived DCs showed a significant reduction in the level of HLA-DR (P≤0.05), CD86 (P≤0.05), CD80 (P≤0.001), CD83 (P≤0.001), and CD1a. Also, the expression of IDO (P≤0.001) and IL10 increased while IL12 expression decreased (P≤0.001).Conclusion: Our findings revealed that tolerogenic probiotics could induce regulatory DCs by reducing co-stimulatory molecules along with increasing the expression of IDO and IL10 during the differentiation process. Therefore, the induced regulatory DCs probably can be used in the treatment of various inflammatory diseases.","PeriodicalId":54921,"journal":{"name":"Iranian Journal of Immunology","volume":"20 1","pages":"26-35"},"PeriodicalIF":0.9,"publicationDate":"2023-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9234084","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Predictive Value of the Serum Diamine Oxidase Level in the Diagnosis of Seasonal Allergic Rhinitis. 血清二胺氧化酶水平对季节性变应性鼻炎诊断的预测价值。

IF 0.9 4区医学

Iranian Journal of Immunology Pub Date : 2022-12-01 DOI: 10.22034/IJI.2022.95336.2358

Ali Aghili, Ahmad Rezaeian

{"title":"Predictive Value of the Serum Diamine Oxidase Level in the Diagnosis of Seasonal Allergic Rhinitis.","authors":"Ali Aghili, Ahmad Rezaeian","doi":"10.22034/IJI.2022.95336.2358","DOIUrl":"https://doi.org/10.22034/IJI.2022.95336.2358","url":null,"abstract":"Background: Allergic rhinitis (AR) is characterized by the increased sensitivity of the nasal mucosa to allergens and has a significant impact on life quality. There is promising evidence that biomarkers can help in the diagnosis, treatment, and follow-up of patients with AR. Diamine oxidase (DAO) is one of the enzymes responsible for the breakdown of histamine, the primary mediator of allergies.Objective: To investigate the significance of DAO as a useful biomarker for diagnosis and the severity of AR.Methods: In this case-control study, 24 patients and 24 healthy controls were recruited and their serum DAO levels, total IgE levels (using ELISA), blood eosinophil count, and percentage (using complete blood cell count) were measured. The sino-nasal outcomes test-22 (SNOT-22) questionnaire was used to assess the severity of symptoms in patients. The Receiver Operating Characteristic (ROC) analysis was used to assess the predictive power of DAO level for the diagnosis of AR. The relationship between DAO and disease severity, as well as other AR-related clinical factors, were also investigated.Results: DAO levels were lower in AR patients compared with the controls. The DAO level did not significantly correlate with the severity of AR according to the Allergic Rhinitis and its Impact on Asthma (ARIA) score, though it was lower in patients with persistent or moderate to severe symptoms. The total IgE, eosinophil percentage, and SNOT-22 score all had an inverse relationship with DAO. Moreover, DAO was significantly associated with the diagnosis of AR, with an Area under the ROC Curve (AUC) of 0.771, a sensitivity of 75%, and a specificity of 62.5%.Conclusion: DAO might be a valuable biomarker in the diagnosis of allergic rhinitis.","PeriodicalId":54921,"journal":{"name":"Iranian Journal of Immunology","volume":"19 4","pages":"395-403"},"PeriodicalIF":0.9,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10832428","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of Innate Lymphoid Cells (ILCs) Population in the Mouse Model of Colorectal Cancer. 结肠直肠癌小鼠模型中先天淋巴样细胞(ILCs)群体的评价。

IF 0.9 4区医学

Iranian Journal of Immunology Pub Date : 2022-12-01 DOI: 10.22034/IJI.2022.92467.2152

Seyed Mohammad Javadzadeh, Mohsen Keykhosravi, Mohsen Tehrani, Hossein Asgarian-Omran, Mohsen Rashidi, Hadi Hossein-Nattaj, Laleh Vahedi-Larijani, Abolghasem Ajami

{"title":"Evaluation of Innate Lymphoid Cells (ILCs) Population in the Mouse Model of Colorectal Cancer.","authors":"Seyed Mohammad Javadzadeh, Mohsen Keykhosravi, Mohsen Tehrani, Hossein Asgarian-Omran, Mohsen Rashidi, Hadi Hossein-Nattaj, Laleh Vahedi-Larijani, Abolghasem Ajami","doi":"10.22034/IJI.2022.92467.2152","DOIUrl":"https://doi.org/10.22034/IJI.2022.92467.2152","url":null,"abstract":"BACKGROUND Innate Lymphoid Cells (ILCs) promote tissue homeostasis, contribute to the immune defense mechanisms, and play important roles in the initiation of immune responses and chronic inflammation. OBJECTIVE To understand the roles of innate lymphoid cells in the pathophysiology of colorectal cancer (CRC) in the mouse model. METHODS CRC was induced using azoxymethane (AOM) and dextran sulfate sodium (DSS) in Balb/c mice (the chemically induced group=18 mice), or orthotopic injection of CT-26 cell line into the colon of another set of Balb/c mice (the orthotopic group=14mice). Normal saline was injected into 18 mice, as the sham group. After 80 days, the chemically induced group was divided into two subgroups, dysplasia (8 mice) and reparative change (10 mice), based on pathological examinations. The frequencies of ILC1, 2, and 3 were then measured in colon tissues using flow cytometry by four markers including an anti-mouse lineage cocktail (FITC anti-mCD3/FITC anti-mGr-1/FITC anti-mCD11b/ FITC anti-mCD45R (B220)/FITC anti-mTer-119), PE/Cy7 anti-mouse CD45, PE anti-mouse CD117 (c-kit), and APC anti-mouse IL-33 Rα (ST2). RESULTS The total ILC population was significantly higher in the chemically induced reparative change compared with the sham group. ILC1 percentage in the chemically induced reparative change was significantly higher compared to those in the other three groups (Sham, chemically induced dysplasia and orthotopic dysplasia). The orthotopic dysplasia group showed more ILC3 percentage than the other groups. CONCLUSION ILC1 and ILC3 subgroups increased significantly in reparative and dysplastic experimental CRC respectively. Thus ILC1 may have an inhibitory effect on tumor growth whereas ILC3 promotes tumor progression.","PeriodicalId":54921,"journal":{"name":"Iranian Journal of Immunology","volume":"19 4","pages":"339-348"},"PeriodicalIF":0.9,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10537169","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Evaluation of CD3+ T Cell Percentage, Function and its Relationship with Serum Vitamin D Levels in Women with Recurrent Spontaneous Abortion and Recurrent Implantation Failure. 反复自然流产和反复植入失败妇女CD3+ T细胞百分比、功能及其与血清维生素D水平的关系

IF 0.9 4区医学

Iranian Journal of Immunology Pub Date : 2022-12-01 DOI: 10.22034/IJI.2022.91464.2083

Abdolah Mousavi Salehi, Mehri Ghafourian, Afshin Amari, Mahvash Zargar

{"title":"Evaluation of CD3+ T Cell Percentage, Function and its Relationship with Serum Vitamin D Levels in Women with Recurrent Spontaneous Abortion and Recurrent Implantation Failure.","authors":"Abdolah Mousavi Salehi, Mehri Ghafourian, Afshin Amari, Mahvash Zargar","doi":"10.22034/IJI.2022.91464.2083","DOIUrl":"https://doi.org/10.22034/IJI.2022.91464.2083","url":null,"abstract":"Background: Women afflicted with recurrent spontaneous abortion (RSA) and repeated implantation failure (RIF) may have immune abnormalities. The role of vitamin D has been demonstrated in the function of the immune system.Objective: To assess the percentage and function of CD3+ T cells and their relationship with the level of the serum vitamin D or 1,25-dihydroxy vitamin D3 (the active form of the vitamin) in women with RSA and RIF.Methods: In this case-control study, peripheral blood was obtained from the patient and the healthy control groups. The ratio of CD3+T cell and activated CD3+ CD69+T cell was investigated using flow cytometry. The serum levels of Interferon-γ (IFN-γ) and vitamin D were measured by ELISA.Results: The mean proportion of CD3+T cells in women with RSA increased significantly compared with the healthy control group (p<0.04). However, no significant difference was observed in RIF women compared with the control group. There was no significant difference in the ratio of activated CD3+CD69+T cells between the patient and the healthy control groups. Serum IFN-γ levels in women with RSA showed a significant increase compared to the control group (p<0.031); however, no significant difference was observed between women with RIF and the control group. Serum levels of vitamin D showed a significant reduction in both RSA (p<0.01) and RIF (p<0.04) groups in comparison with the control.Conclusion: An increase in the percentage and inflammatory function of T cells was associated with RSA. Decreased vitamin D levels may contribute to immune dysfunction and pregnancy loss.","PeriodicalId":54921,"journal":{"name":"Iranian Journal of Immunology","volume":"19 4","pages":"369-377"},"PeriodicalIF":0.9,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10457290","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0