The Upregulation of HLA-G1 and miRNA-34a in Lens Epithelial Cells of Diabetic Retinopathy Patients.

IF 1.1 4区医学 Q4 IMMUNOLOGY

Iranian Journal of Immunology Pub Date : 2023-05-31 DOI:10.22034/iji.2023.97403.2510

Elnaz Taghvaei-Bijandi, Fatemeh Abedian, Ahmad Ahmadzadeh Amiri, Narjes Jafari, Saeid Abediankenari

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引用次数: 0

Abstract

Background: Retinopathy of diabetes is a chronic diabetes mellitus complication affecting retinal vessels, and some ocular complications' molecular mechanisms remain obscure.

Objective: To evaluate the expression of HLA-G1, HLA-G5, miRNA-181a, and miRNA-34a in the lens epithelial cells of patients with retinopathy of diabetes.

Methods: In a case-control study, 30 diabetic patients with retinopathy, 30 diabetic patients without retinopathy, and 30 cataract patients without diabetes mellitus as the control group were enrolled after a full description with details about the study methods and objectives. The expression of HLA G1, HLA G5, miRNA-181a, and miRNA-34a in lens epithelial cells was assessed by quantitative RT PCR. Moreover, the levels of HLA-G protein in aqueous humor were evaluated by the ELISA method.

Results: HLA-G1 expression was significantly upregulated in the retinopathy group (P=0.003). The aqueous humor of diabetic retinopathy patients contained significantly higher levels of HLA-G protein compared with the non-diabetic patients (P=0.001). miRNA-181a was significantly downregulated in the diabetic retinopathy group compared with the patients without diabetes (P=0.001). In addition, miRNA-34a was upregulated in the retinopathy group (P=0.009).

Conclusion: Taken together, the present results showed that HLA-G1 and miRNA-34a can be valuable markers for diabetic retinopathy. Our data offers new perspectives for improving the control of inflammation in the lens epithelial cells by considering HLA-G and miRNA.

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糖尿病视网膜病变患者晶状体上皮细胞中HLA-G1和miRNA-34a的上调

背景:糖尿病视网膜病变是一种影响视网膜血管的慢性糖尿病并发症，一些眼部并发症的分子机制尚不清楚。目的:探讨糖尿病视网膜病变患者晶状体上皮细胞中HLA-G1、HLA-G5、miRNA-181a、miRNA-34a的表达情况。方法:采用病例对照研究，在详细说明研究方法和目的后，选取30例合并视网膜病变的糖尿病患者、30例无视网膜病变的糖尿病患者和30例无糖尿病的白内障患者作为对照组。采用定量RT - PCR法检测晶状体上皮细胞HLA G1、HLA G5、miRNA-181a、miRNA-34a的表达。采用ELISA法测定房水中HLA-G蛋白水平。结果:视网膜病变组HLA-G1表达明显上调(P=0.003)。糖尿病视网膜病变患者房水中HLA-G蛋白水平明显高于非糖尿病患者(P=0.001)。与非糖尿病患者相比，miRNA-181a在糖尿病视网膜病变组中显著下调(P=0.001)。此外，视网膜病变组miRNA-34a表达上调(P=0.009)。结论:综上所述，HLA-G1和miRNA-34a可作为糖尿病视网膜病变有价值的标志物。我们的数据为通过考虑HLA-G和miRNA来改善晶状体上皮细胞炎症的控制提供了新的视角。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Iranian Journal of Immunology Medicine-Immunology and Allergy

CiteScore

1.60

自引率

0.00%

发文量

审稿时长

12 weeks

期刊介绍： The Iranian Journal of Immunology (I.J.I) is an internationally disseminated peer-reviewed publication and publishes a broad range of experimental and theoretical studies concerned with all aspects of immunology.