Iranian Journal of Immunology最新文献_第8页

The Generation and Application of Monoclonal Antibodies to Detect SAPCD2 Expression in Precancerous and Malignant Gastric Lesions. 单克隆抗体检测胃癌前及恶性病变中SAPCD2表达的制备及应用

IF 0.9 4区医学

Iranian Journal of Immunology Pub Date : 2023-05-31 DOI: 10.22034/iji.2023.97498.2519

Fang Gao, Yuan Chen, Wenwei Zhang, Xiaolong Wang, Jingchun Li, Feng Feng, Chunli Liu, Wenxi He, Wei Sun

{"title":"The Generation and Application of Monoclonal Antibodies to Detect SAPCD2 Expression in Precancerous and Malignant Gastric Lesions.","authors":"Fang Gao, Yuan Chen, Wenwei Zhang, Xiaolong Wang, Jingchun Li, Feng Feng, Chunli Liu, Wenxi He, Wei Sun","doi":"10.22034/iji.2023.97498.2519","DOIUrl":"https://doi.org/10.22034/iji.2023.97498.2519","url":null,"abstract":"Background: Suppressor APC domain containing 2 (SAPCD2) is involved in cell cycle regulation and its mRNA levels are higher in cancer tissues. But, the function of SAPCD2 in cancer development remains unclear.Objective: To generate mouse monoclonal antibodies (mAbs) specific to SAPCD2 and thus clarify the function of SAPCD2 in the development of gastric carcinoma (GC). Methods: Purified SAPCD2-GST immunized BALB/c mouse spleen cells were collected and fused with myeloma cells to obtain monoclonal antibody hybridoma. A group of monoclonal antibodies exhibiting high specificity and sensitivity against SAPCD2 has been generated and characterized by IHC, WB, IP, IF, and ELISA. By immunohistochemical analysis, the SAPCD2 expression was evaluated in 228 clinical samples of gastric mucosal lesions, including precancerous lesions and GC samples.Results: We identified a highly specific and sensitive clone of s12 in eukaryotic cells and performed an IHC analysis. We found that 81.3% of 107 GC tissues were SAPCD2-positive, compared with the 26.2% in the matched adjacent normal-appearing tissues (P<0.001). Furthermore, among the 121 gastritis tissues, SAPCD2 was overexpressed in precancerous gastric lesions such as dysplasia (Dys, 78.9%), intestinal metaplasia (IM, 44.7%), and chronic atrophic gastritis (CAG, 6.1%) compared with that in chronic non-atrophic gastritis (CNAG, 3.2%) (P<0.001). The SAPCD2-positivity rate was 81.3% in GC, suggesting that the expression of SAPCD2 increased with the severity of the lesion (P<0.001).Conclusion: In summary, we have described novel monoclonal antibodies against SAPCD2, which are highly expressed in GC tissues and may serve as the basis for an early clinical marker for GC development.","PeriodicalId":54921,"journal":{"name":"Iranian Journal of Immunology","volume":"20 2","pages":"190-201"},"PeriodicalIF":0.9,"publicationDate":"2023-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9973138","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Long-Term Effects of Low-Dose Naltrexone on Immunomodulatory Properties of Human Adipose-Derived Mesenchymal Stem Cells. 低剂量纳曲酮对人脂肪源性间充质干细胞免疫调节特性的长期影响。

IF 0.9 4区医学

Iranian Journal of Immunology Pub Date : 2023-05-31 DOI: 10.22034/iji.2023.95659.2385

Romina Kazemi, Mobin Mohammadi, Samira Salimiyan, Sara Aliakbari, Moslem Ahmadi, Rahmani Mohammad Reza

{"title":"Long-Term Effects of Low-Dose Naltrexone on Immunomodulatory Properties of Human Adipose-Derived Mesenchymal Stem Cells.","authors":"Romina Kazemi, Mobin Mohammadi, Samira Salimiyan, Sara Aliakbari, Moslem Ahmadi, Rahmani Mohammad Reza","doi":"10.22034/iji.2023.95659.2385","DOIUrl":"https://doi.org/10.22034/iji.2023.95659.2385","url":null,"abstract":"Background: Low-dose naltrexone (LDN) is involved in the treatment of inflammatory and immune system diseases and can affect immune cells. Mesenchymal stem cells (MSCs) are known for their immunomodulatory effects and the potential for the treatment of certain types of autoimmune diseases.Objective: To investigate the long-term effects of LDN on human adipose-derived mesenchymal stem cells (ASCs) to see how their immunomodulatory properties are affected and also how LDN-treated ASCs interact with other immune cells present in peripheral blood mononuclear cells (PBMCs).Methods: After 14 days of treatment, the ability of LDN-treated ASCs to modulate PBMC proliferation in a two-way mixed lymphocyte reaction (MLR) model was assessed using XTT. The relative expression of IDO, PD-L1, COX-2, HGF genes, and the level of IL-6 and TGF-β cytokines were measured in IFN-γ stimulated and unstimulated ASCs (treated and not treated cells) using real-time PCR and ELISA respectively.Results: Unstimulated ASCs treated with 10-8 M Naltrexone (10-8 M NTX) showed higher levels of TGF-β, compared with the controls (P<0.05). Stimulated ASCs treated with 10-6 M NTX showed elevated expression of IDO, PD-L1 genes, and IL-6 level (P<0.05).Conclusion: Our results demonstrated that various LDN concentrations have dissimilar effects on ASCs' immunomodulatory properties. A higher LDN concentration induced an alteration in the immunomodulatory features of ASCs.","PeriodicalId":54921,"journal":{"name":"Iranian Journal of Immunology","volume":"20 2","pages":"219-231"},"PeriodicalIF":0.9,"publicationDate":"2023-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9551265","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pulmonary Arterial Hypertension Induced by Immune Checkpoint Inhibitor Combined Therapy in a Patient with Intrahepatic Cholangiocarcinoma: A Case Report. 免疫检查点抑制剂联合治疗肝内胆管癌致肺动脉高压1例

IF 0.9 4区医学

Iranian Journal of Immunology Pub Date : 2023-05-31 DOI: 10.22034/iji.2023.96898.2464

Jingjing Zhang, Shasha Zhang, Shuo Xu, Xiaoyun Zhang, Jiasong Li, Zhengzheng Ji, Qingyi Liu, Zhanjun Guo

{"title":"Pulmonary Arterial Hypertension Induced by Immune Checkpoint Inhibitor Combined Therapy in a Patient with Intrahepatic Cholangiocarcinoma: A Case Report.","authors":"Jingjing Zhang, Shasha Zhang, Shuo Xu, Xiaoyun Zhang, Jiasong Li, Zhengzheng Ji, Qingyi Liu, Zhanjun Guo","doi":"10.22034/iji.2023.96898.2464","DOIUrl":"https://doi.org/10.22034/iji.2023.96898.2464","url":null,"abstract":"Case: Immune Checkpoint Inhibitors (ICIs) have dramatically revolutionized the therapeutic approaches by which we treat a series of cancers accompanied by immune-related adverse events (irAEs). Herein, we reported an intrahepatic cholangiocarcinoma male patient with a history of ankylosing spondylitis developing pulmonary arterial hypertension (PAH) under ICI combined therapy with pembrolizumab and lenvatinib. The indirect measurement of cardiac ultrasound showed a pulmonary artery pressure (PAP) of 72mmHg after 21 three-week cycles of ICI combined therapy. The patient partially responded to the treatment of glucocorticoid and mycophenolate mofetil. The PAP decreased to 55mmHg 3 months after the ICI combined therapy was discontinued, but increased to 90mmHg after the ICI combined therapy was rechallenged. We treated him with adalimumab -an antitumor necrosis factor-alpha (ani-TNF-α) antibody- combined with glucocorticoid and immunosuppressants under lenvatinib monotherapy. The patient responded again with PAP decreasing to 67mmHg after 2 two-week cycles of adalimumab. Accordingly, we diagnosed him to have irAE-related PAH. Our findings supported the use of glucocorticoid disease-modifying antirheumatic drugs (DMARDs) as a treatment option in refractory PAH.","PeriodicalId":54921,"journal":{"name":"Iranian Journal of Immunology","volume":"20 2","pages":"240-246"},"PeriodicalIF":0.9,"publicationDate":"2023-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9543750","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of CD137 and CD137L Transcript Levels and the Serum sCD137 in Immune-mediated Polyneuropathy. 免疫介导的多发性神经病中CD137和CD137L转录物水平及血清sCD137的评价

IF 0.9 4区医学

Iranian Journal of Immunology Pub Date : 2023-03-14 DOI: 10.22034/iji.2023.96695.2453

Shirin Torkestani, Alireza Zamani, Mehrdokht Mazdeh, Elahe Talebi-Ghane, Ghodratoallah Roshanaie, Mohammad Mahdi Eftekharian

{"title":"Evaluation of CD137 and CD137L Transcript Levels and the Serum sCD137 in Immune-mediated Polyneuropathy.","authors":"Shirin Torkestani, Alireza Zamani, Mehrdokht Mazdeh, Elahe Talebi-Ghane, Ghodratoallah Roshanaie, Mohammad Mahdi Eftekharian","doi":"10.22034/iji.2023.96695.2453","DOIUrl":"https://doi.org/10.22034/iji.2023.96695.2453","url":null,"abstract":"Background: Abnormal humoral and cellular immune responses have been reported in immune-mediated polyneuropathies. CD137, as a costimulatory molecule and a TNF receptor superfamily member, has been demonstrated to have a key role in the pathogenesis of many autoimmune as well as inflammatory disorders.Objective: To evaluate the transcripts levels of CD137, its ligand (CD137L), and the serum levels of soluble CD137 (sCD137) in patients with immune-mediated polyneuropathy.Methods: A total of 45 patients and 46 sex and age-matched healthy individuals were enrolled in the study. CD137 and CD137L transcript levels were assessed by the Real-Time PCR, and the serum level of sCD137 was measured using the ELISA technique. The Bayesian regression model was used for statistical analysis at the 0.05 significance level in R 4.1.0 statistical environment.Results: Transcript levels of the CD137 and CD137L were higher in polyneuropathy patients in comparison with the healthy subjects (P=0.006 for both). Conversely, the mean level of sCD137 was significantly lower in the sera of patients compared to the controls (P<0.001).Conclusion: Our findings point to the possible role of CD137 and CD137L in immune-mediated polyneuropathy pathogenesis. More investigations are required to clarify the exact contributions of the mentioned molecules to the pathogenesis of immune-mediated polyneuropathies.","PeriodicalId":54921,"journal":{"name":"Iranian Journal of Immunology","volume":"20 1","pages":"104-113"},"PeriodicalIF":0.9,"publicationDate":"2023-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9239074","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Anti-inflammatory Mechanisms of IL-38 in Chinese Patients with Allergic Rhinitis. 中国变应性鼻炎患者IL-38的抗炎机制。

IF 0.9 4区医学

Iranian Journal of Immunology Pub Date : 2023-03-14 DOI: 10.22034/iji.2023.94361.2305

Xiaoqiang Wang, Shen Yang, Xia Ke, Suling Hong

{"title":"Anti-inflammatory Mechanisms of IL-38 in Chinese Patients with Allergic Rhinitis.","authors":"Xiaoqiang Wang, Shen Yang, Xia Ke, Suling Hong","doi":"10.22034/iji.2023.94361.2305","DOIUrl":"https://doi.org/10.22034/iji.2023.94361.2305","url":null,"abstract":"Background: T-helper 17 (Th17) cell response is engaged in the onset of allergic rhinitis (AR). Moreover, interleukin (IL)-38 is thought to be involved in inhibiting cytokine secretion in the Th17 pathway.Objective: To evaluate the regulatory function of IL-38 on abnormal Th17 responses in Chinese patients with AR.Methods: Forty-five participants, divided into an AR group (n=25) and a control group (n=20), were recruited for the study. In addition, the expression of IL-38 and Th17-related cytokines was measured as well as the Th17 cell count in participants. By implementing recombinant IL-38 (rIL-38), the intervention of human peripheral blood mononuclear cells (PBMCs) was performed. Then, flow cytometry, polymerase chain reaction (PCR), and enzyme-linked immunosorbent assay (ELISA) were used to detect the Th17 milieu.Results: The expression of IL-38 in the AR group notably reduced compared with that in the control, whereas Th17 cell frequency and the expression levels of its transcription factor (RORC) and cytokines (IL-17A and IL-23) increased. The differentiation and immune function of Th17 cells in PBMCs were inhibited by rIL-38.Conclusion: Th17 responses are inhibited by IL-38 in patients with AR. Therefore, the obtained findings indicate that IL-38 is a potential therapeutic target for Chinese patients with AR.","PeriodicalId":54921,"journal":{"name":"Iranian Journal of Immunology","volume":"20 1","pages":"92-103"},"PeriodicalIF":0.9,"publicationDate":"2023-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9232954","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Exploring the Influence of Hepatitis B Immunoglobulin on the Immune Response to the Hepatitis B Vaccine in a Mouse Model. 探讨乙型肝炎免疫球蛋白对小鼠乙肝疫苗免疫应答的影响。

IF 0.9 4区医学

Iranian Journal of Immunology Pub Date : 2023-03-14 DOI: 10.22034/iji.2023.92402.2156

Tao Wei, Wei-Hong Lv, Mei-Hua Gao, Shan-Juan Tan, Ling Li, Lei Zhang

{"title":"Exploring the Influence of Hepatitis B Immunoglobulin on the Immune Response to the Hepatitis B Vaccine in a Mouse Model.","authors":"Tao Wei, Wei-Hong Lv, Mei-Hua Gao, Shan-Juan Tan, Ling Li, Lei Zhang","doi":"10.22034/iji.2023.92402.2156","DOIUrl":"https://doi.org/10.22034/iji.2023.92402.2156","url":null,"abstract":"Background: The extent to which maternal antibodies against the hepatitis B surface antigen (HBsAb) acquired transplacentally affect the immune responses to the hepatitis B vaccine (HBVac) in infants is still uncertain.Objective: To explore the impact of the HBsAb on the immune response to the HBVac in a mouse model.Methods: According to the doses of the HBVac (2, 5 μg) injected, 267 BALB/c mice were divided into two groups. Each group was subdivided into 3 subgroups based on the doses of the hepatitis B immunoglobulin (HBIG) (0, 25, 50 IU) administered. The HBsAb titers were detected 4 weeks after completing the HepB vaccination.Results: Among all the mice, 40 had an HBsAb titer <100 mIU/mL (non- or low-response to the HBVac). The rates of the HBsAb titer <100 mIU/mL in 0, 25 and 50 IU HBIG groups were 1.1%, 23.1%, and 20.7%, respectively. Multivariate logistic regression analysis showed that the risk factors for low- or non-response to the HBVac were injection with the HBIG, low HBVac dose, and hypodermic injection. The mean HBsAb titers (log10) reduced gradually in the 0, 25 and 50 IU HBIG groups (P<0.001).Conclusion: The HBIG administration has negative impacts on the peak level of the HBsAb and the rate of an effective immune response. This implies that the maternal HBsAb acquired transplacentally might inhibit the immune responses to the HBVac in infants.","PeriodicalId":54921,"journal":{"name":"Iranian Journal of Immunology","volume":"20 1","pages":"67-75"},"PeriodicalIF":0.9,"publicationDate":"2023-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9239069","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Immunogenicity of the Non-toxic Form of Staphylococcus Aureus α-Hemolysin Using a Chimeric Fusion in Mice. 利用小鼠嵌合融合研究无毒型金黄色葡萄球菌α-溶血素的免疫原性

IF 0.9 4区医学

Iranian Journal of Immunology Pub Date : 2023-03-14 DOI: 10.22034/iji.2023.82351.1575

Liquan Yu, Jian Dai, Fan Ziyao, Liang Chen, Daolong Liu, Zhijun Wu, Hunan Sun, Chunyu Tong, Yongzhong Yu, Baifen Song, Jinzhu Ma, Yudong Cui

{"title":"Immunogenicity of the Non-toxic Form of Staphylococcus Aureus α-Hemolysin Using a Chimeric Fusion in Mice.","authors":"Liquan Yu, Jian Dai, Fan Ziyao, Liang Chen, Daolong Liu, Zhijun Wu, Hunan Sun, Chunyu Tong, Yongzhong Yu, Baifen Song, Jinzhu Ma, Yudong Cui","doi":"10.22034/iji.2023.82351.1575","DOIUrl":"https://doi.org/10.22034/iji.2023.82351.1575","url":null,"abstract":"Background: Staphylococcus aureus is an opportunistic pathogen responsible for various infections with diverse clinical presentation and severity. The α-hemolysin is a major virulence factor in the pathogenesis of S. aureus infections.Objective: To produce a chimeric fusion protein for hemolytic detection of the S. aureus isolates and as a component of a multi-antigen vaccine.Methods: The fused strategy employed a flexible linker to incorporate the possible B cell and T cell determinants into one chimera (HlaD). The humoral and cellular response to the HlaD in mice was assessed to reveal a non-significant difference compared with the full-length α-hemolysin mutant (Hla H35L).Results: The results of the protective effect, the mimetic lung cell injury, and bacterial clearness demonstrated that the mice vaccinated with the HlaD alleviated the severity of the infection of the S. aureus, and the HlaD could similarly function with Hla H35L.Conclusion: The chimeric fusion (HlaD) provided a diagnostic antigen for hemolysis of the S. aureus strains and a potential vaccine component.","PeriodicalId":54921,"journal":{"name":"Iranian Journal of Immunology","volume":"20 1","pages":"57-66"},"PeriodicalIF":0.9,"publicationDate":"2023-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9239068","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Leukocyte Reduction Filters Are Reliable and Economic Source for Natural Killer Cell Preparation. 白细胞还原过滤器是制备自然杀伤细胞的可靠和经济的来源。

IF 0.9 4区医学

Iranian Journal of Immunology Pub Date : 2023-03-14 DOI: 10.22034/iji.2023.92527.2158

Moosa Rezwani, Abdulbaset Mazarzaei, Zahra Abbasi-Malati, Ali Akbar Pourfathollah

{"title":"Leukocyte Reduction Filters Are Reliable and Economic Source for Natural Killer Cell Preparation.","authors":"Moosa Rezwani, Abdulbaset Mazarzaei, Zahra Abbasi-Malati, Ali Akbar Pourfathollah","doi":"10.22034/iji.2023.92527.2158","DOIUrl":"https://doi.org/10.22034/iji.2023.92527.2158","url":null,"abstract":"Background: An issue that hinders researchers' access to Natural Killer (NK) cells is their low proportion in peripheral blood leukocytes. This issue is currently addressed by methods involving a series of differentiation and expansions that are time-consuming and expensive.Objective: We have investigated whether the used leukocyte reduction filters, a by-product in the blood transfusion practice that currently is considered waste, can be utilized as a source of the NK cells.Methods: Following the blood donation of 46 donors based on the Iranian Blood Transfusion Organization's protocols, a sample of peripheral blood of each donor and the leukocyte reduction filter used in their donation procedure have been obtained. The entrapped cells were flushed back from the leukocyte reduction filters. Both groups of samples were analyzed using an automatic hematological analyzer. NK cell isolation was done by the MACS negative selection method. The samples have been comparatively analyzed utilizing flow cytometry data of NK cells' subpopulation compositions, viability, degranulation patterns, and cytotoxic capacity against the K562 cell line.Results: Every major leukocyte population was abundant in the samples extracted from the used leukocyte reduction filters. The NK cells extracted from leukocyte reduction filters did not show any statistically meaningful differences (P<0.5) from peripheral blood samples in terms of subpopulation composition, viability, degranulation potency, and cytotoxic capacity.Conclusion: Used leukocyte reduction filters can be considered an economic, easy to obtain, and robust source of abundant research-grade NK cells.","PeriodicalId":54921,"journal":{"name":"Iranian Journal of Immunology","volume":"20 1","pages":"114-128"},"PeriodicalIF":0.9,"publicationDate":"2023-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9239077","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Maternal Cold-Reacting Immunoglobulin G Anti-M of MNS Blood Group System Causing Hemolytic Disease of the Fetus. 母体冷反应免疫球蛋白G抗m引起胎儿溶血性疾病的MNS血型系统。

IF 0.9 4区医学

Iranian Journal of Immunology Pub Date : 2023-03-14 DOI: 10.22034/iji.2023.93993.2273

Yan-Lian Liang, Yu Shi, Yu-Qing Su, Fan Wu, Yanwen Liang, Xiuchu Fan, Jiansuo Lin, Yi Liu, Peng Long, Jianwei Ren, Shuang Liang

引用次数: 0

Effect of the Previous History of the SARS-CoV-2 Infections on Antibody Levels among Sputnik V Vaccinated Healthcare Workers. SARS-CoV-2感染史对接种过Sputnik V疫苗的医护人员抗体水平的影响

IF 0.9 4区医学

Iranian Journal of Immunology Pub Date : 2023-03-14 DOI: 10.22034/iji.2023.93812.2260

Atieh Yaghoubi, Samira Asli, Maryam Parhizkar, Maryam Mohammadpour, Ali Khorsand, Mehdi Yousefi, Taravat Bamdad, Saeid Amel Jamehdar

{"title":"Effect of the Previous History of the SARS-CoV-2 Infections on Antibody Levels among Sputnik V Vaccinated Healthcare Workers.","authors":"Atieh Yaghoubi, Samira Asli, Maryam Parhizkar, Maryam Mohammadpour, Ali Khorsand, Mehdi Yousefi, Taravat Bamdad, Saeid Amel Jamehdar","doi":"10.22034/iji.2023.93812.2260","DOIUrl":"https://doi.org/10.22034/iji.2023.93812.2260","url":null,"abstract":"Background: Measuring the level of antibodies produced post-vaccination in response to the SARS-CoV-2 spike protein is considered a strategy for estimating the effectiveness of the COVID-19 vaccines.Objective: To examine the antibody levels among the healthcare workers in different hospitals in Mashhad, Iran after receiving the second dose of Sputnik V.Methods: In this study, we enrolled 230 healthcare workers for evaluating the Gam-COVID-Vac or Sputnik V after the second administration in different hospitals in Mashhad. Antibody levels of spike protein were quantitatively evaluated in a sample of 230 negative RT-PCR tests for the COVID-19 individuals. The analysis has been done based on an immunological assay using enzyme-linked immunosorbent assay (ELISA). The infection history of the subjects and their families was examined through their medical records.Results: Our results demonstrated a significant association between a higher titer of IgG and a previous history of the COVID-19 infection (P<0.001). Moreover, the chance of detecting antibodies titer more than 50 AU/ml was 16.99 in these people which was significantly higher than in people without a history of infection pre-vaccination [%95CI: (7.38,39.12), P<0.001].Conclusion: This result demonstrates that the efficacy of antibody production is related to the previous history of the SARS-CoV-2 infections. Ongoing monitoring of the level of antibody among vaccinated populations will help evaluating the effect of vaccines on humoral immunity status.","PeriodicalId":54921,"journal":{"name":"Iranian Journal of Immunology","volume":"20 1","pages":"76-82"},"PeriodicalIF":0.9,"publicationDate":"2023-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9232955","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0