Iranian Journal of Immunology最新文献_第10页

Introducing a New Method for Purification of Human IL-4 by Substitution of a Single Amino Acid in IL-4 Protein Sequence. 介绍了一种通过替换IL-4蛋白序列中单个氨基酸纯化人IL-4的新方法。

IF 0.9 4区医学

Iranian Journal of Immunology Pub Date : 2022-12-01 DOI: 10.22034/IJI.2022.94985.2336

Mohsen Mazloomrezaei, Mahsa Sadat Hosseini, Nahid Ahmadi, Elham Mahmoudi Maymand, Ebrahim Eftekhar, Amir Asgari, Amin Ramezani

{"title":"Introducing a New Method for Purification of Human IL-4 by Substitution of a Single Amino Acid in IL-4 Protein Sequence.","authors":"Mohsen Mazloomrezaei, Mahsa Sadat Hosseini, Nahid Ahmadi, Elham Mahmoudi Maymand, Ebrahim Eftekhar, Amir Asgari, Amin Ramezani","doi":"10.22034/IJI.2022.94985.2336","DOIUrl":"https://doi.org/10.22034/IJI.2022.94985.2336","url":null,"abstract":"Background: It is advantageous to develop an effective purification procedure to produce recombinant protein drugs (rPDs) without any tags. To remove N- or C-terminus tags from the rPDs, several cleavage site-based endopeptidases were used. Separating the endopeptidase enzyme from the rPDs is a time-consuming and costly process.Objective: To design and develop a new method for the purification of human interleukin (IL)-4 with potential application for other cytokines.Methods: Met-like amino acids were substituted at position 120 to reduce the possibility of alteration in the structure of IL-4 and its biological activity. Based on the in silico analysis, isoleucine was chosen as an alternative amino acid, and the M120I mutant IL-4 (mIL-4) model was selected for the downstream analysis. Recombinant mIL-4 was produced in the E.coli BL21 host and purified with CNBr. Then in vitro evaluations of the native and mutant IL-4 were performed.Results: The results showed that both the native and mutant IL-4 had the same effect on TF-1 cell proliferation. On the other hand, there was no significant difference between the effects of native IL-4 (nIL-4) and mIL-4 on the expression of IL-4 and IL-10 in activated peripheral blood mononuclear cells. Native and mutant IL-4 have similar biological activities.Conclusion: Here, an efficient and straightforward system is introduced to purify IL-4 cytokine using CNBr, which could be applied to other rPDs.","PeriodicalId":54921,"journal":{"name":"Iranian Journal of Immunology","volume":"19 4","pages":"436-445"},"PeriodicalIF":0.9,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10832431","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Changes in Maternal Serum Levels of C3 and C4 Complement Components in Different Delivery Methods and Postpartum Hemorrhage. 不同分娩方式及产后出血孕妇血清C3、C4补体成分水平的变化

IF 0.9 4区医学

Iranian Journal of Immunology Pub Date : 2022-12-01 DOI: 10.22034/IJI.2022.93655.2242

Guangxia Cui, Tingyue Zhang, Hongjiang Tian, Hui Zhang, Jin Zhang, Xi Wang, Xiaoyan Zhang, Wenpei Bai

{"title":"Changes in Maternal Serum Levels of C3 and C4 Complement Components in Different Delivery Methods and Postpartum Hemorrhage.","authors":"Guangxia Cui, Tingyue Zhang, Hongjiang Tian, Hui Zhang, Jin Zhang, Xi Wang, Xiaoyan Zhang, Wenpei Bai","doi":"10.22034/IJI.2022.93655.2242","DOIUrl":"https://doi.org/10.22034/IJI.2022.93655.2242","url":null,"abstract":"Background: Activation of the complement system may play a role in the pathophysiology of human labor. Yet no unanimous conclusion has been drawn.Objective: To compare the differences in maternal complement components C3 and C4 serum levels in cesarean section and the vaginal delivery at term and in the postpartum hemorrhage.Methods: One hundred and sixty six women delivered at term were enrolled in this study. Maternal blood samples were obtained from 47 cases of elective cesarean section and 119 cases of the vaginal delivery. Serum complement levels were measured subsequently by immuno-scatter turbidimetry.Results: The maternal complement levels declined significantly during delivery by both the cesarean section and the vaginal delivery (p＜0.01) in comparison with the baseline. A much larger drop of C3 serum level was found in the postpartum hemorrhage and in the vaginal delivery, and the incidence of the postpartum hemorrhage has a positive correlation with the complement decline rate.Conclusion: The complement system may be involved in the delivery process and represents a predictive value in postpartum hemorrhage.","PeriodicalId":54921,"journal":{"name":"Iranian Journal of Immunology","volume":"19 4","pages":"378-384"},"PeriodicalIF":0.9,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10457294","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Effect of Oral Administration of Silymarin on Serum Levels of Tumor Necrosis Factor-α and Interleukin-1ß in Patients with Rheumatoid Arthritis. 口服水飞蓟素对类风湿关节炎患者血清肿瘤坏死因子-α和白细胞介素-1ß水平的影响。

IF 0.9 4区医学

Iranian Journal of Immunology Pub Date : 2022-12-01 DOI: 10.22034/IJI.2022.90456.2007

Mehrdad Shavandi, Yasaman Yazdani, Shirin Asar, Arash Mohammadi, Ehsan Mohammadi-Noori, Amir Kiani

{"title":"The Effect of Oral Administration of Silymarin on Serum Levels of Tumor Necrosis Factor-α and Interleukin-1ß in Patients with Rheumatoid Arthritis.","authors":"Mehrdad Shavandi, Yasaman Yazdani, Shirin Asar, Arash Mohammadi, Ehsan Mohammadi-Noori, Amir Kiani","doi":"10.22034/IJI.2022.90456.2007","DOIUrl":"https://doi.org/10.22034/IJI.2022.90456.2007","url":null,"abstract":"Background: Rheumatoid Arthritis (RA) is a systemic chronic autoimmune disease. Several inflammatory agents play key roles in RA pathogenesis, among which tumor necrosis factor-alpha (TNF-α) and interleukin 1 beta (IL-1β) are of great importance. Silymarin is a potent anti-oxidant extracted from Silybummarianum L. seeds.Objective: To study the effect of silymarin on serum levels of TNF-α and IL-1β in patients with RA.Methods: Patients with stable RA received 140 mg of silymarin, 3 times a day, for 3 months. Serum samples were collected before and after the treatment. Both TNF-α and IL-1β serum levels were measured by ELISA.Results: 42 patients (14.3% male, and 85.7% female, with a mean age of 47.59±12.8 years old) completed the treatment course. There was no significant difference in the overall mean concentration of either TNF-α (p=0.14) or IL-1β (p=0.27) in all 42 patients after the treatment with silymarin.Conclusion: The addition of silymarin to the treatment regimen of patients with stable RA has no significant effect on the serum levels of TNF-α and IL-1β, however, this study needs further evaluation with a larger sample size.","PeriodicalId":54921,"journal":{"name":"Iranian Journal of Immunology","volume":"19 4","pages":"427-435"},"PeriodicalIF":0.9,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10457289","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

The Impact of HLA-G and HLA-E Polymorphisms on CMV Reinfection in Liver Transplant Recipients. 肝移植受者中 HLA-G 和 HLA-E 多态性对 CMV 再感染的影响

IF 0.9 4区医学

Iranian Journal of Immunology Pub Date : 2022-12-01 DOI: 10.22034/IJI.2022.95345.2359

Masumeh Darai, Saeede Soleimanian, Ramin Yaghobi, Kourosh Kazemi, Saman Nikeghbalian, Negar Azarpira

{"title":"The Impact of HLA-G and HLA-E Polymorphisms on CMV Reinfection in Liver Transplant Recipients.","authors":"Masumeh Darai, Saeede Soleimanian, Ramin Yaghobi, Kourosh Kazemi, Saman Nikeghbalian, Negar Azarpira","doi":"10.22034/IJI.2022.95345.2359","DOIUrl":"10.22034/IJI.2022.95345.2359","url":null,"abstract":"Background: Cytomegalovirus (CMV) reinfection in transplant patients has been associated with graft loss and decreased patient survival. In this regard, the HLA-G molecule has the immunomodulatory characteristic and its soluble isoforms have important roles in immunity to viruses. The 14bp insertion/deletion polymorphism impacts HLA-G mRNA stability. Regarding the HLA-E molecule, two nonsynonymous alleles, HLA-E*0101, and HLA-E*0103 are different in their functions including the affinity of the relative peptide.Objective: To explore the possible link between HLA-G and HLA-E polymorphisms with CMV reinfection among liver transplant recipients (LTRs).Methods: In this study, a total of 140 liver transplantations were performed; of which 70 CMV-reactivated LTRs and 70 CMV non-reactivated ones were recruited. The cut-off value of CMV DNA was determined to be 100 copies/mL. PCR evaluated different genotypes for HLA-G and ARMS-PCR for HLA-E*0101 and *0103.Results: Neither the HLA-G genotypes (-14 bp/-14bp and +14bp/+14 bp homozygous genotypes with the p-values: 0.43, and 0.13, respectively +14 bp⁄-14 bp heterozygous genotype with p-value: 0.49) nor the HLA-E genotypes (HLA-E*0101/0103, HLA-E*0101/0101, and HLA-E*0103/0103 with the p-values: 0.152, 0.249, and 0.391, respectively) had any association with CMV reinfection in the LTRs.Conclusion: No difference was observed in the HLA-E and HLA-G genotype frequencies between our studied groups. Further studies are needed to explore other genetic variations and evaluate soluble HLA-G and HLA-E levels in the transplant population.","PeriodicalId":54921,"journal":{"name":"Iranian Journal of Immunology","volume":"19 4","pages":"404-413"},"PeriodicalIF":0.9,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10832430","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Niclosamide Modulates the Cellular and Humoral Immune Response in Balb/c Mice. 氯胺调节Balb/c小鼠的细胞和体液免疫反应。

IF 0.9 4区医学

Iranian Journal of Immunology Pub Date : 2022-12-01 DOI: 10.22034/IJI.2022.90653.2022

Bilal Mahmood Beg, Aqeel Javeed, Muhammad Ashraf, Arfan Ahmad, Adeel Sattar, Mehmood Ahmad

{"title":"Niclosamide Modulates the Cellular and Humoral Immune Response in Balb/c Mice.","authors":"Bilal Mahmood Beg, Aqeel Javeed, Muhammad Ashraf, Arfan Ahmad, Adeel Sattar, Mehmood Ahmad","doi":"10.22034/IJI.2022.90653.2022","DOIUrl":"https://doi.org/10.22034/IJI.2022.90653.2022","url":null,"abstract":"Background: Niclosamide, a STAT3 inhibitor, is widely under investigation due to its anti-cancer properties. STAT3 also exhibits an exciting role in the immune responses.Objective: This study aimed to evaluate the impact of niclosamide on immune response of mice.Methods: Niclosamide was administered to balb/c mice. To evaluate cell-mediated immune response, a contact-hypersensitivity (CHS) test, cyclophosphamide-induced neutropenic assay, and carbon clearance test were performed, whereas a humoral immune response was evaluated by hemagglutination assay (HA) and mice lethality test. The concentration of TGF-β1 was determined by enzyme-linked immunosorbent assay (ELISA) on murine peritoneal macrophages.Results: In the CHS test, niclosamide caused a decrease in skin thickness, significantly exhibiting a decrease in inflammation. A highly significant decrease in overall leukocyte count (lymphocytes and neutrophils) was observed before and after cyclophosphamide injection as compared with the control group. However, only a highly significant decrease in the neutrophil percentage was observed. Niclosamide has decreased the phagocytic process immensely compared with the control. In the HA titer, niclosamide was found to reduce the antibodies' titer compared with the negative control group. In the mice lethality test, the treatment groups have shown an increase in the percentage of mortality. TGF-β1 elevated in peritoneal macrophages when treated with niclosamide, in a dose-dependent manner.Conclusion: Niclosamide exerts potent immunomodulatory effects by significantly suppressing cell-mediated and humoral immune responses and increasing the levels of TGF-β1 in mice. Niclosamide might be added as an adjuvant to immunosuppressive drugs for the treatment of autoimmune diseases.","PeriodicalId":54921,"journal":{"name":"Iranian Journal of Immunology","volume":"19 4","pages":"414-426"},"PeriodicalIF":0.9,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10457295","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Letter to the Editor in Response to the "Comparison of the Antibody Responses Following Vaccination with AstraZeneca and Sinopharm". 就“阿斯利康与国药疫苗接种后抗体反应的比较”致编辑的信。

IF 0.9 4区医学

Iranian Journal of Immunology Pub Date : 2022-12-01 DOI: 10.22034/IJI.2022.96908.2466

Rujittika Mungmunpuntipantip, Viroj Wiwanitkit

引用次数: 0

LncRNA-GAS5 and its Promoter Region Polymorphism Associate with Helper Th17 Polarization and Predict Postoperative Pain and the Prognosis of the Patients with Hepatocellular Carcinoma Undergoing Hepatectomy. LncRNA-GAS5及其启动子区多态性与辅助性Th17极化相关并预测肝细胞癌切除术患者术后疼痛和预后

IF 0.9 4区医学

Iranian Journal of Immunology Pub Date : 2022-12-01 DOI: 10.22034/IJI.2022.92678.2168

Zhanming Sha, Panpan Zhang

{"title":"LncRNA-GAS5 and its Promoter Region Polymorphism Associate with Helper Th17 Polarization and Predict Postoperative Pain and the Prognosis of the Patients with Hepatocellular Carcinoma Undergoing Hepatectomy.","authors":"Zhanming Sha, Panpan Zhang","doi":"10.22034/IJI.2022.92678.2168","DOIUrl":"https://doi.org/10.22034/IJI.2022.92678.2168","url":null,"abstract":"Background: The relationship between genetic polymorphism and postoperative pain and the prognosis of patients with hepatocellular carcinoma (HCC) undergoing hepatectomy is not fully understood.Objective: To examine whether lncRNA-GAS5 and its promoter region rs145204276 polymorphism can predict postoperative pain and prognosis of the patients with HCC undergoing hepatectomy.Methods: Seventy patients with HCC undergoing hepatectomy were enrolled. The lncRNA-GAS5 levels in CD4+ T cells from peripheral blood mononuclear cells (PBMC-CD4+ T cells) and tumor tissues were measured by qRT-PCR. Genotyping analysis of rs145204276 was performed using the TaqMan platform. PBMC-CD4+ T cells were isolated and the cytokine levels in helper T (Th) cells were determined by flow cytometry. Patients with Ins/Ins genotype carrying the rs145204276 polymorphism were allocated into the Ins group, and others were allocated into the Del group.Results: The lncRNA-GAS5 level decreased significantly in PBMC-CD4+ T cells and tumor tissues compared with the healthy controls and corresponding adjacent non-tumor tissues. The patients with Del/Del genotype showed significantly higher lncRNA-GAS5 expression in PBMC-CD4+ T cells, lower postoperative pain scores, and better overall survival. LncRNA-GAS5 expression in PBMC-CD4+ T cells was negatively associated with IL-6, IL-17, and the RORγT/CD3 ratio (an indicator of Th17 polarization).Conclusion: LncRNA-GAS5 expression and its promoter region rs145204276 polymorphism are prognostic biomarkers that can predict postoperative pain of patients with HCC undergoing hepatectomy.","PeriodicalId":54921,"journal":{"name":"Iranian Journal of Immunology","volume":"19 4","pages":"349-357"},"PeriodicalIF":0.9,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10466814","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification of the CD16low CD56low CD38pos Natural Killer Cell as a Key Subset in Patients with Multiple Myeloma. CD16low CD56low CD38pos自然杀伤细胞在多发性骨髓瘤患者中的关键亚群鉴定

IF 0.9 4区医学

Iranian Journal of Immunology Pub Date : 2022-12-01 DOI: 10.22034/IJI.2022.93965.2269

Ting Chen, Zhenghong Yu

{"title":"Identification of the CD16low CD56low CD38pos Natural Killer Cell as a Key Subset in Patients with Multiple Myeloma.","authors":"Ting Chen, Zhenghong Yu","doi":"10.22034/IJI.2022.93965.2269","DOIUrl":"https://doi.org/10.22034/IJI.2022.93965.2269","url":null,"abstract":"Background: Natural killer (NK) cells are classified as innate immune cells which can directly recognize and kill tumor cells without antigen sensitization. NK cell-based adoptive immunotherapy for blood malignancies has attracted more attention in recent years.Objective: To analyze different NK cell subsets in the peripheral blood and bone marrow (BM) of patients with multiple myeloma (MM).Methods: Using flow cytometry we analyzed: (i) the distribution of distinct NK cell subpopulations (i.e. CD16low CD56low, CD16pos CD56high, CD16neg CD56high, CD16high CD56low, CD16neg CD56low, CD16low CD56low CD38pos) in the BM from MM patients at distinct disease stages. (ii) the expression of NKG2D, DNAM-1 and NKp30, and (iii) the expression of CD107a in CD16low CD56low CD38pos and CD16low CD56low CD38neg NK cells subsets.Results: CD16low CD56low CD38pos was the dominant subset in BM from patients with MM at the CR stage with a decreased expression of NKp30. CD16low CD56low CD38pos subset showed a higher proportion of CD107a expression compared to CD16low CD56low CD38neg cells. In vitro experiments indicated that the CD16low CD56low CD38pos NK cell subset possesses more cytotoxicity than CD16low CD56low CD38neg NK cells.Conclusion: Our data suggest that CD16low CD56low CD38pos NK cells may reflect as an effector population with the potential therapeutic target in patients with MM. This group of cells may be useful for adoptive immunotherapy in MM in the future.","PeriodicalId":54921,"journal":{"name":"Iranian Journal of Immunology","volume":"19 4","pages":"358-368"},"PeriodicalIF":0.9,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10457291","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enzyme-Assisted Extraction of Narirutin from Citri Reticulatae Pericarpium and Anti-allergic Asthma Activity. 柑橘皮中纳瑞素的酶辅助提取及抗过敏性哮喘活性研究。

IF 0.9 4区医学

Iranian Journal of Immunology Pub Date : 2022-12-01 DOI: 10.22034/IJI.2022.91640.2095

Xiaolei Shi, Lina Zhao, Liyan Niu, Jihao Wei, Xuwen Li

{"title":"Enzyme-Assisted Extraction of Narirutin from Citri Reticulatae Pericarpium and Anti-allergic Asthma Activity.","authors":"Xiaolei Shi, Lina Zhao, Liyan Niu, Jihao Wei, Xuwen Li","doi":"10.22034/IJI.2022.91640.2095","DOIUrl":"https://doi.org/10.22034/IJI.2022.91640.2095","url":null,"abstract":"Background: Asthma is a heterogeneous disorder of the airways related to inflammation; it affects millions of people worldwide. Due to the side effects of inhaled corticosteroids, researchers focused on the therapeutic effects of compounds derived from natural products.Objective: To investigate the therapeutic benefits of Narirutin a valuable flavonoid in Citri Reticulatae Pericarpium for asthma.Methods: Narirutin was extracted using the enzyme-assisted method with the L9 (34) orthogonal array to optimize the temperatures, pH, and reaction time. The mechanism of action of Narirutin was investigated via ELISA, flow cytometry, and Western blot analysis in vivo.Results: Narirutin suppressed inflammatory cell infiltration in the lung tissue and decreased IgE and IgG1 levels in serum in vivo. It can also alleviate interleukin (IL)-4, IL-5, and interferon-γ concentrations in bronchoalveolar lavage fluid in mice. Moreover, it increased the ratio of CD4+/CD8+ T cells. Additionally, Narirutin significantly suppressed p-ERK1/2 and p-JNK expression in the MAPK signaling pathway.Conclusion: Narirutin affects the Th1/Th2 imbalance through the p-ERK and p-JNK suppression in the MAPK signaling pathway.","PeriodicalId":54921,"journal":{"name":"Iranian Journal of Immunology","volume":"19 4","pages":"385-394"},"PeriodicalIF":0.9,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10832429","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparison of the Antibody Responses Following Vaccination with AstraZeneca and Sinopharm. 阿斯利康与国药疫苗接种后抗体反应的比较。

IF 0.9 4区医学

Iranian Journal of Immunology Pub Date : 2022-09-01 DOI: 10.22034/iji.2022.94298.2300

Enayat Anvari, Atefe Ghamar Talepoor, Mahsa Eshkevar Vakili, Narges Karimi, MohammadReza Ataollahi, Gelareh Najafi, Dieter Kabalitz, Iraj Ahmadi, Kurosh Kalantar

{"title":"Comparison of the Antibody Responses Following Vaccination with AstraZeneca and Sinopharm.","authors":"Enayat Anvari, Atefe Ghamar Talepoor, Mahsa Eshkevar Vakili, Narges Karimi, MohammadReza Ataollahi, Gelareh Najafi, Dieter Kabalitz, Iraj Ahmadi, Kurosh Kalantar","doi":"10.22034/iji.2022.94298.2300","DOIUrl":"https://doi.org/10.22034/iji.2022.94298.2300","url":null,"abstract":"Background: Vaccines are the most effective way to prevent Coronavirus 2 severe acute respiratory syndrome (SARS-CoV-2).Objectives: To compare the antibody response of healthy individuals vaccinated with either the AstraZeneca (ChAdOx1 nCoV-19) or the Sinopharm (BBIBP-CorV) vaccine, in those who had no prior infection with SARS-CoV-2.Methods: Thirty seven participants were included, of which 17 were administered the AstraZeneca (ChAdOx1 nCoV-19) vaccine, while 20 were given the Sinopharm (BBIBP-CorV) vaccine. SARS-CoV-2 neutralizing antibody and anti-receptor-binding domain (RBD) IgG levels were checked 4 weeks after giving the first and the second dose of either vaccine using the enzyme-linked immunosorbent assay (ELISA) technique.Results: The AstraZeneca (ChAdOx1 nCoV-19) vaccine exhibited a higher levels of anti-(RBD) IgG compared with the Sinopharm (BBIBP-CorV) in both the first (14.51 μg/ml vs. 1.160 μg/ml) and the second (46.68 μg/ml vs. 11.43 μg/ml) doses. About neutralizing Abs, the titer of the antibody was higher in the AstraZeneca (ChAdOx1 nCoV-19) recipients than in the Sinopharm (BBIBP-CorV) subjects after the first (7.77 μg/ml vs. 1.79 μg/ml, p < 0.0001) and the second dose (10. 36 μg/ml vs. 4.88 μg/ml, p < 0.0001).Conclusions: Recipients vaccinated with two doses of the AstraZeneca (ChAdOx1 nCoV-19) had superior quantitative antibody levels than Sinopharm (BBIBP-CorV)-vaccinated subjects. These data suggest that a booster dose may be needed for the Sinopharm (BBIBP-CorV) recipients, to control the COVID-19 pandemic.","PeriodicalId":54921,"journal":{"name":"Iranian Journal of Immunology","volume":"19 3","pages":"321-329"},"PeriodicalIF":0.9,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9271968","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2