Iranian Journal of Immunology最新文献_第2页

Interleukin-14 Prevents Cytarabine or Irradiation Induced Neutropenia via JAK/STAT3 Signaling. 白细胞介素-14通过JAK/STAT3信号阻止阿糖胞苷或辐照诱导的中性粒细胞减少。

IF 1.1 4区医学

Iranian Journal of Immunology Pub Date : 2025-06-23 DOI: 10.22034/iji.2025.105019.2925

Shengnan Xu, Chaoqiong Ye, Zhihua Wu, Chuying Wen, Wenhui Huang, Cong Deng, Jiaye Hua, Wei Xie, Min Xiao

{"title":"Interleukin-14 Prevents Cytarabine or Irradiation Induced Neutropenia via JAK/STAT3 Signaling.","authors":"Shengnan Xu, Chaoqiong Ye, Zhihua Wu, Chuying Wen, Wenhui Huang, Cong Deng, Jiaye Hua, Wei Xie, Min Xiao","doi":"10.22034/iji.2025.105019.2925","DOIUrl":"10.22034/iji.2025.105019.2925","url":null,"abstract":"Background: Severe neutropenia significantly increases the risk of bacterial infections. Recent studies have shown that the cytokine interleukin 14 (IL-14) plays an important role in immune cells, but its potential role in neutropenia induced by cytarabine (ara-c) or irradiation is unclear.Objective: To investigate the role of IL-14 in ara-c or irradiation-induced neutropenia.Methods: Two neutropenia models were induced by ara-c or irradiation. Neutrophil count was confirmed through flow cytometry and routine blood tests. IL-14 was used to assess the impact on neutropenia. IL-14 expression was analyzed using qPCR, Western blotting and ELISA. A IL-14 receptor (IL-14R) knockout mice model was utilized to confirm the role of IL-14R/STAT3 signaling in vivo.Results: The results indicated that IL-14 treatment promoted proliferation and increased neutrophil counts in both bone marrow and peripheral blood, while IL-14R knockout suppressed this process. Furthermore, the downstream molecule of IL-14R, STAT3, showed enhanced phosphorylation levels in the presence of IL-14. Finally, we explored the source of IL-14 in the bone marrow, and found that lymphocytes secreted the highest levels of IL-14. Serum levels of IL-14 were significantly reduced in patients after chemotherapy.Conclusions: These results indicate that IL-14 prevents ara-c or irradiation-induced neutropenia by regulating lymphocytes and activating the IL-14R/STAT3 pathway in neutrophils. This evidence suggests that IL-14 is a potent cytokine for treating ara-c or irradiation-induced neutropenia.","PeriodicalId":54921,"journal":{"name":"Iranian Journal of Immunology","volume":"22 2","pages":"106-118"},"PeriodicalIF":1.1,"publicationDate":"2025-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144369597","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Hypomethylation of OAS2 and OAS3 Gene Promoters: Insights into the ‎Pathogenesis of Systemic Lupus Erythematosus. OAS2和OAS3基因启动子的低甲基化：对系统性红斑狼疮发病机制的见解。

IF 1.1 4区医学

Iranian Journal of Immunology Pub Date : 2025-06-23 DOI: 10.22034/iji.2025.105409.2944

Esmat Rigi Yousefabadi, Zahra Ourang, Farhad Gharibdoost, Seyedeh Tahereh Faezi, Mohammad Saatchi, Delnya Gholami, Emran Esmaeilzadeh, Hamid Reza Khorram Khorshid

{"title":"Hypomethylation of OAS2 and OAS3 Gene Promoters: Insights into the ‎Pathogenesis of Systemic Lupus Erythematosus.","authors":"Esmat Rigi Yousefabadi, Zahra Ourang, Farhad Gharibdoost, Seyedeh Tahereh Faezi, Mohammad Saatchi, Delnya Gholami, Emran Esmaeilzadeh, Hamid Reza Khorram Khorshid","doi":"10.22034/iji.2025.105409.2944","DOIUrl":"10.22034/iji.2025.105409.2944","url":null,"abstract":"Background: DNA methylation plays a key role in systemic lupus erythematosus (SLE) by regulating gene expression and impacting immune system functions. In SLE, abnormal DNA methylation patterns can lead to the overexpression of pro-inflammatory genes and downregulation of the regulatory genes, contributing to autoimmunity. This dysregulation can increase susceptibility to SLE. Understanding these methylation changes could help discover new therapeutic strategies for managing SLE.Objective: To evaluate methylation levels of OAS2 and OAS3 in peripheral blood mononuclear cells (PBMCs) in volunteers with SLE were evaluated.Methods: In this case-control study, we collected 207 peripheral blood samples from 102 SLE patients and 105 healthy subjects. After isolating the PBMCs, methylation analysis was performed using the methylation-quantification of endonuclease-resistant DNA (MethyQESD) method.Results: The control group had an average OAS2 methylation percentage of 40.02% ± 24.59%, whereas the SLE group had a significantly lower average of 19.46% ± 21.98%. This finding indicates a significant hypomethylation of OAS2 in the SLE cohort (P<0.001). Additionally, a significant difference was observed in the mean methylation levels of OAS3, with SLE patients exhibiting 14.11% ± 19.50% compared to healthy controls at 25.32% ± 20.82% (P<0.001). Patients with renal damage also showed significantly lower OAS2 methylation levels than SLE individuals without renal damage (P<0.001). Furthermore, a negative connection was found between the OAS2 methylation level and creatinine (r= -0.266, P= 0.007).Conclusion: The pattern of methylation levels observed in OAS2 and OAS3 within PBMCs may provide valuable insights into the mechanisms underlying SLE development.","PeriodicalId":54921,"journal":{"name":"Iranian Journal of Immunology","volume":"22 2","pages":"155-164"},"PeriodicalIF":1.1,"publicationDate":"2025-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144369596","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Modulation of the CD200/CD200R Axis by IFN-β Treatment in a Mouse Model of Experimental Autoimmune Encephalomyelitis. IFN-β治疗实验性自身免疫性脑脊髓炎小鼠模型中CD200/CD200R轴的调节

IF 1.1 4区医学

Iranian Journal of Immunology Pub Date : 2025-06-09 DOI: 10.22034/iji.2025.104603.2910

Dariush Haghmorad, Arman Rahimmi, Alireza Pazoki, Fatemeh Namazi, Mohammad Reza Rahmani, Abbas Ali Amini

{"title":"Modulation of the CD200/CD200R Axis by IFN-β Treatment in a Mouse Model of Experimental Autoimmune Encephalomyelitis.","authors":"Dariush Haghmorad, Arman Rahimmi, Alireza Pazoki, Fatemeh Namazi, Mohammad Reza Rahmani, Abbas Ali Amini","doi":"10.22034/iji.2025.104603.2910","DOIUrl":"10.22034/iji.2025.104603.2910","url":null,"abstract":"Background: Interferon-b (IFN-β), a glycoprotein released during viral infections, plays a crucial role in modulating T cells involved in multiple sclerosis (MS). CD200 is an immunomodulatory molecule expressed in many cell types, including neurons. It reduces the progression of MS and experimental autoimmune encephalomyelitis (EAE) by interacting with CD200R, mainly expressed on myeloid lineage cells. This interaction prevents brain damage and slows the progression of the disease.Objective: This study investigated changes in the expression of CD200 and CD200R genes in the brains of mice induced with EAE.Methods: Female C57B/L6 mice were divided into three distinct groups: 1) EAE-induced and treated with IFN-b, 2) EAE-induced and treated with phosphate-buffered saline (PBS), and 3) a healthy control group. Two weeks after treatment, the mice were euthanized, and whole-brain tissues were used for mRNA extraction. After cDNA synthesis, the expression of CD200 and CD200R genes was evaluated using Taqman Real-Time PCR. Leukocyte infiltration and demyelination were assessed using Hematoxylin and Eosin staining (H&E) as well as Luxol fast blue (LFB).Results: IFN-β treatment significantly reduced disease progression and demyelination. Furthermore, mice treated with IFN-β showed improved weight gain. The findings also indicated no notable change in CD200 gene expression across the groups examined. However, the expression of CD200R decreased in the IFN-β-treated group, but significantly increased in the untreated group.Conclusion: Our findings suggest that IFN-β treatment may decrease CD200R expression by reducing inflammation. Additionally, the elevated expression in the untreated group may explain why EAE is self-limiting.","PeriodicalId":54921,"journal":{"name":"Iranian Journal of Immunology","volume":"22 2","pages":"131-141"},"PeriodicalIF":1.1,"publicationDate":"2025-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144250968","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Altered Serum IL-35 Levels and IL-37 Gene Expression in Patients with Parkinson's Disease: Focus on Emerging Cytokines. 帕金森病患者血清IL-35水平和IL-37基因表达的改变：关注新兴细胞因子

IF 1.1 4区医学

Iranian Journal of Immunology Pub Date : 2025-06-08 DOI: 10.22034/iji.2025.105508.2955

Fariba Akbari Gavabari, Mohsen Rastegari-Pouyani, Saied Afshar, Mehrdokht Mazdeh, Elaheh Talebi-Ghane, Mohammad Mahdi Eftekharian

{"title":"Altered Serum IL-35 Levels and IL-37 Gene Expression in Patients with Parkinson's Disease: Focus on Emerging Cytokines.","authors":"Fariba Akbari Gavabari, Mohsen Rastegari-Pouyani, Saied Afshar, Mehrdokht Mazdeh, Elaheh Talebi-Ghane, Mohammad Mahdi Eftekharian","doi":"10.22034/iji.2025.105508.2955","DOIUrl":"10.22034/iji.2025.105508.2955","url":null,"abstract":"Background: Parkinson's disease (PD) is increasingly recognized as a condition driven by both central and peripheral inflammatory responses, largely mediated by cytokine activity.Objective: To assess IL-35 (P35 and Ebi3 subunits) and IL-37 gene expression, along with the serum levels of IL-35 protein in patients with PD compared to healthy controls.Methods: Cytokine gene expression was measured using the qRT-PCR technique, while IL-35 serum levels were measured using the ELISA method. The data obtained were analyzed using a Bayesian regression model in the R software.Results: The results revealed that the expression of P35 gene, of the two subunits of IL-35, did not differ significantly between the two groups. However, Ebi3 and IL-37 transcript levels were significantly lower in patients compared to healthy individuals (p<0.001). In contrast, IL-35 serum level in patients showed a significant increase compared to the control group (p=0.016). Notably, IL-37 expression showed a negative correlation with age (p=0.004). . We also observed positive and significant correlations between the gene expression of P35 and Ebi3 (p= 0.02, r= 0.4), P35 and IL-37 (p= 0.008, r= 0.45), and Ebi3 and IL-37 (p= 0.016, r= 0.41).Conclusion: In conclusion, our study revealed a higher serum protein level of IL-35 in PD patients compared to the healthy control group. Meanwhile, gene expression levels of IL-37 and Ebi-3 were significantly reduced. These alterations in the expression of these cytokines are suggested to be partly responsible for the immune system dysregulation in this disease.","PeriodicalId":54921,"journal":{"name":"Iranian Journal of Immunology","volume":"22 2","pages":"142-154"},"PeriodicalIF":1.1,"publicationDate":"2025-06-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144250967","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Does Endoplasmic Reticulum (ER) Stress Contribute to T-cell Exhaustion in B-ALL? 内质网（ER）应激是否有助于B-ALL的t细胞衰竭？

IF 1.1 4区医学

Iranian Journal of Immunology Pub Date : 2025-05-26 DOI: 10.22034/iji.2025.105453.2949

Amir Kahrizi, Armin Akbar, Ahmad Najafi, Hossein Asgarian-Omran, Hossein Karami, Mohammad Naderisorki, Alireza Karimi, Mohsen Tehrani

{"title":"Does Endoplasmic Reticulum (ER) Stress Contribute to T-cell Exhaustion in B-ALL?","authors":"Amir Kahrizi, Armin Akbar, Ahmad Najafi, Hossein Asgarian-Omran, Hossein Karami, Mohammad Naderisorki, Alireza Karimi, Mohsen Tehrani","doi":"10.22034/iji.2025.105453.2949","DOIUrl":"10.22034/iji.2025.105453.2949","url":null,"abstract":"Background: Glucose deprivation in T lymphocytes can trigger compensatory metabolic pathways, potentially contributing to T-cell exhaustion. Additionally, it may induce the unfolded protein response (UPR), ultimately resulting in endoplasmic reticulum (ER) stress.Objective: To examine the transcriptional profiles of endoplasmic reticulum (ER) stress markers and T-cell exhaustion indicators in CD8+ T lymphocytes isolated from B-ALL patients.Methods: Peripheral blood samples were collected from 22 untreated B-ALL patients and 22 healthy controls. Magnetic Activated Cell Sorting (MACS) was used to isolate CD8+ T lymphocytes. The relative gene expression was then assessed using qRT-PCR with primers specific to XBP1, CHOP, GLUT1, and T-bet.Results: The ER stress response was significantly activated in CD8+ T lymphocytes from B-ALL patients, as evidenced by significant increase in both XBP1 and CHOP transcript levels, relative to normal donors. Although GLUT1 mRNA expression was significantly higher than in control groups, T-bet expression showed no significant difference between the two groups.Conclusion: Collectively, our gene expression data suggest ER stress activation in CD8+ T lymphocytes from B-ALL patients. These findings warrant further investigation into ER stress-related signaling pathways and their potential role in promoting T-cell exhaustion in B-ALL.","PeriodicalId":54921,"journal":{"name":"Iranian Journal of Immunology","volume":"22 2","pages":"100-105"},"PeriodicalIF":1.1,"publicationDate":"2025-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144144512","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Interaction Between Tfh/Tfr Ratio and Regulatory B Cell in Autoimmune Diseases. 自身免疫性疾病中Tfh/Tfr比值与调节性B细胞的相互作用

IF 1.1 4区医学

Iranian Journal of Immunology Pub Date : 2025-03-30 DOI: 10.22034/iji.2025.103848.2859

Chunhong Zhu, Xiaoying Ni, Jiangming Xu, Hao Wang, Hongqiang Shen

{"title":"Interaction Between Tfh/Tfr Ratio and Regulatory B Cell in Autoimmune Diseases.","authors":"Chunhong Zhu, Xiaoying Ni, Jiangming Xu, Hao Wang, Hongqiang Shen","doi":"10.22034/iji.2025.103848.2859","DOIUrl":"10.22034/iji.2025.103848.2859","url":null,"abstract":"The balance between follicular helper T cells (Tfh) and follicular regulatory T cells (Tfr) is crucial for maintaining immune tolerance. Tfh cells are key in producing autoantibodies by providing essential help to germinal center (GC) B cells, while Tfr cells prevent autoimmune inflammatory processes by controling Tfh responses. However, the signals that regulate Tfh and Tfr cells are largely unknown. Due to dysregulated Tfr/Tfh balance and autoantibody production, regulatory B cells (Bregs) have emerged as a key checkpoint in the GC response. Bregs are B cells with immunosuppressive capabilities. Significant advancements have been made in understanding the roles of Bregs, particularly their capacity to produce cytokines with anti-inflammatory properties and regulate Th17, Th1, and regulatory T cells (Tregs) in the context of autoimmune conditions. Bregs also play a pivotal role in shaping the development, regulation, and localization of Tfh and Tfr cells within the immune environment. Consequently, gaining mechanistic knowledge about the interactions between Tfh-Bregs and Tfr-Bregs has the potential to establish homeostasis and suppress the development of autoantibodies in a various disorders. Within the context of autoimmune disorders, this article provides a concise summary of the dysregulation of Tfh/Tfr, highlighting the critical role of Bregs in regulating this balance. The previously unrecognized interplay between Bregs and Tfh/Tfr cells will serve as an essential basis for the comprehension and management of autoimmune illnesses. It also promises to offer invaluable knowledge of the biological mechanisms of autoantibody synthesis.","PeriodicalId":54921,"journal":{"name":"Iranian Journal of Immunology","volume":"22 1","pages":"1-12"},"PeriodicalIF":1.1,"publicationDate":"2025-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143588313","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of Dendritic Cell Subpopulations Frequency in COVID-19 Patients and their Correlation with Disease Severity. COVID-19患者树突状细胞亚群频率的评估及其与疾病严重程度的相关性

IF 1.1 4区医学

Iranian Journal of Immunology Pub Date : 2025-03-30 DOI: 10.22034/iji.2025.104236.2887

Vahid Asghariazar, Majid Eterafi, Somaieh Matin, Nasrin Fouladi, Rozita Abolhasani, Monireh Falsafi, Afshin Fathi, Elham Safarzadeh

{"title":"Evaluation of Dendritic Cell Subpopulations Frequency in COVID-19 Patients and their Correlation with Disease Severity.","authors":"Vahid Asghariazar, Majid Eterafi, Somaieh Matin, Nasrin Fouladi, Rozita Abolhasani, Monireh Falsafi, Afshin Fathi, Elham Safarzadeh","doi":"10.22034/iji.2025.104236.2887","DOIUrl":"10.22034/iji.2025.104236.2887","url":null,"abstract":"Background: COVID-19 (2019) clearly demonstrates an imbalanced immune response. Variations in the function and subtypes of dendritic cells (DCs) may have effects on immune responses in COVID-19 patients and contribute to immunopathology.Objective: To assess the phenotype and frequency of Plasmacytoid dendritic cells (pDCs), Conventional DCs (cDCs), and double-positive DCs in COVID-19 patients admitted to the ICU and non-ICU compared to the healthy control group.Methods: The study included 10 healthy individuals and 25 COVID-19 patients. In the second week of their illness, Peripheral blood mononuclear cells (PBMCs) were isolated from the patients and labeled with targeted antibodies for HLA-DR, CD123, and CD11c. The samples were then analyzed using flow cytometry. The COVID-19 patients were divided into two ICU and non-ICU groups and were closely monitored throughout the study.Results: In comparison to healthy controls, COVID-19 patients exhibited a significantly lower pDCs ratio (P=0.04). Patients were categorized into two groups: (A) the ICU group (n=11; 44%) and (B) the non-ICU group (n=14; 56%). The frequency of pDC was significantly lower in ICU patients than in non-ICU patients (P<0.01). Although not statistically significant, ICU patients had a lower frequency of cDCs and double positive DCs compared to non-ICU patients. Additionally, a significant association between the age of COVID-19 patients and cDC levels was observed (p=0.049).Conclusion: SARS-CoV-2 can evade attacks from the immune response by reducing the number of DCs and suppressing their function of DCs, ultimately resulting in weakened development of both innate and adaptive immunity.","PeriodicalId":54921,"journal":{"name":"Iranian Journal of Immunology","volume":"22 1","pages":"70-82"},"PeriodicalIF":1.1,"publicationDate":"2025-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143702370","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

CX3CR1 Functions as a Biomarker Associated with Pathological Tumor Staging in ‎the Diagnosis and Prognosis of Prostate Cancer. CX3CR1在前列腺癌诊断和预后中作为与病理肿瘤分期相关的生物标志物

IF 1.1 4区医学

Iranian Journal of Immunology Pub Date : 2025-03-30 DOI: 10.22034/iji.2025.104018.2876

Shiquan Xu, Qing Liu, Jie Wang, Zeyun Zhang, Ying Wang, Li Li, Tao Zhang, Yu Fan

{"title":"CX3CR1 Functions as a Biomarker Associated with Pathological Tumor Staging in ‎the Diagnosis and Prognosis of Prostate Cancer.","authors":"Shiquan Xu, Qing Liu, Jie Wang, Zeyun Zhang, Ying Wang, Li Li, Tao Zhang, Yu Fan","doi":"10.22034/iji.2025.104018.2876","DOIUrl":"10.22034/iji.2025.104018.2876","url":null,"abstract":"Background: Previous research has identified several potential biomarkers associated with pathological ‎tumor (pT) staging in prostate cancer (PCa) patients. Among these biomarkers, CX3CR1 is ‎notable for its connection to the immune microenvironment.‎.Objective‎: To further investigate the significance of CX3CR1 as a key biomarker for predicting pT ‎staging and PCa progression.‎.Methods‎: Prostate cancer tissue samples were analyzed using quantitative reverse transcription ‎polymerase chain reaction (qRT-PCR) and immunohistochemical staining. The diagnostic ‎performance of CX3CR1 was evaluated using receiver operating characteristic (ROC) curves, ‎while Kaplan-Meier survival analysis was conducted to determine overall survival (OS) rates.‎.Results: A significant decrease in CX3CR1 expression was observed in PCa tissues compared to ‎adjacent normal tissues, with the lowest levels detected in pT3 tumors. CX3CR1 expression ‎showed a negative correlation with preoperative prostate-specific antigen (PSA) levels, lymph ‎node staging (N stage), Gleason score, and overall survival (OS). Additionally, CX3CR1 levels ‎were associated with the polarization of infiltrating CD4+ T cells in PCa patients.‎.Conclusion‎: CX3CR1, as a biomarker associated with pT staging, plays a role in predicting PCa prognosis, ‎potentially by modulating the immune microenvironment.‎.","PeriodicalId":54921,"journal":{"name":"Iranian Journal of Immunology","volume":"22 1","pages":"25-33"},"PeriodicalIF":1.1,"publicationDate":"2025-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143694359","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Low-dose Radiation Improves Tumor Immune Microenvironment, Enhancing the Effects of Anti-CTLA-4 Therapy. 低剂量放疗改善肿瘤免疫微环境，增强抗ctla -4治疗效果。

IF 1.1 4区医学

Iranian Journal of Immunology Pub Date : 2025-03-30 DOI: 10.22034/iji.2025.103258.2825

Jigang Dong, Ying Qi, Sha Sha

{"title":"Low-dose Radiation Improves Tumor Immune Microenvironment, Enhancing the Effects of Anti-CTLA-4 Therapy.","authors":"Jigang Dong, Ying Qi, Sha Sha","doi":"10.22034/iji.2025.103258.2825","DOIUrl":"10.22034/iji.2025.103258.2825","url":null,"abstract":"Background: Radiotherapy destroys tumor cells primarily through direct DNA damage by high-energy particles or indirect DNA damage by free radicals. High-dose radiotherapy (HDR) destroys tumor cells while also damaging normal cells and may potentially cause immunosuppression. The effect of low-dose radiotherapy (LDR) on the tumor microenvironment (TME) may differ from those of HDR.Objective: To determine if combining low-dose radiotherapy with immune checkpoint inhibitors results in synergistic effects.Methods: We established a mouse model for lung cancer and categorized mice into 4 cohorts: NC (negative control) cohort, LDR cohort, anti-CTLA-4 cohort, and LDR+anti-CTLA-4 cohort. Changes in tumor volume were observed in each group, with particular attention given to the variations in immune cells and cytokines within the mouse tumors following LDR.Results: The mice in the LDR+anti-CTLA-4 group exhibited the slowest growth in tumor volume, and low-dose radiotherapy tended to inhibit tumor growth. The proportion of infiltrating CD8+T cells increased and the proportion of infiltrating Treg cells decreased in the tumor after LDR. The levels of interferon (IFN) and the chemokines CXCL9, CXCL10 and CXCL11 were increased after low-dose radiotherapy.Conclusion: LDR has the ability to alter the immune microenvironment of tumors by promoting the production of IFN. Additionally, when combined with anti-CTLA-4, whole-body LDR can effectively suppress tumor growth in mice. The finding is of potential clinical significance and deserves further exploration.","PeriodicalId":54921,"journal":{"name":"Iranian Journal of Immunology","volume":"22 1","pages":"47-57"},"PeriodicalIF":1.1,"publicationDate":"2025-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143588314","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

circ_0001006 Promotes Immune Escape in Non-small Cell Lung Cancer by Regulating the miR-320a/PD-L1 Axis. circ_0001006通过调节miR-320a/PD-L1轴促进非小细胞肺癌的免疫逃逸。

IF 1.1 4区医学

Iranian Journal of Immunology Pub Date : 2025-03-30 DOI: 10.22034/iji.2025.102661.2792

Zhenying Geng, Guoqing Zhang

{"title":"circ_0001006 Promotes Immune Escape in Non-small Cell Lung Cancer by Regulating the miR-320a/PD-L1 Axis.","authors":"Zhenying Geng, Guoqing Zhang","doi":"10.22034/iji.2025.102661.2792","DOIUrl":"10.22034/iji.2025.102661.2792","url":null,"abstract":"Background: Circular RNAs are involved in the tumorigenesis of various tumors, including Non-small cell lung cancer (NSCLC).Objective: To investigate the expression of circ_0001006 in patients with NSCLC and its role in tumorigenesis and immune escape.Methods: A total of 115 patients with NSCLC were enrolled in the study. The expression of circ_0001006 and PD-L1 mRNA were detected using RT-qPCR. Cell proliferation activity, cell migration and invasion abilities were measured using the CCK-8 assay and Transwell chambers assay. Coculture of NSCLC cells with CD8 cytotoxic T cells was conducted to measure the levels of INF-γ, TNF-α, IL-2, and lactate dehydrogenase release in culture supernatants. Bioinformatic analysis was used to predict the target relevance among circ_0001006, miR-320a, and PD-L1.Results: The circ_0001006 and PD-L1 mRNA levels were elevated in NSCLC tissues and cells. Patients with high levels of circ_0001006 had a shorter overall survival rate. Inhibiting circ_0001006 reduced the proliferation, migration, and invasion of NSCLC cells, while increasing PD-L1 partially counteracting the inhibitory effects of si-circ_0001006. The co-culture system of NSCLC and CD8+ T cell was found to reduce the viability of activated CD8+ T cell when circ_0001006 is present. Knocking down circ_0001006 in co-culture cells led to an increase in the expression of INF-γ, TNF-α, and IL-2. The ability of si-circ_0001006 to enhance the activation of CD8+ T cells was diminished when PD-L1 was overexpressed.Conclusion: circ_0001006 may serve as a potential prognostic predictor and therapeutic target for NSCLC. Additionally, it offers insight into a novel regulatory mechanism of circ_0001006.","PeriodicalId":54921,"journal":{"name":"Iranian Journal of Immunology","volume":"22 1","pages":"58-69"},"PeriodicalIF":1.1,"publicationDate":"2025-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143588312","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0