Evaluation of Dendritic Cell Subpopulations Frequency in COVID-19 Patients and their Correlation with Disease Severity.

IF 1.1 4区 医学 Q4 IMMUNOLOGY
Vahid Asghariazar, Majid Eterafi, Somaieh Matin, Nasrin Fouladi, Rozita Abolhasani, Monireh Falsafi, Afshin Fathi, Elham Safarzadeh
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引用次数: 0

Abstract

Background: COVID-19 (2019) clearly demonstrates an imbalanced immune response. Variations in the function and subtypes of dendritic cells (DCs) may have effects on immune responses in COVID-19 patients and contribute to immunopathology.

Objective: To assess the phenotype and frequency of Plasmacytoid dendritic cells (pDCs), Conventional DCs (cDCs), and double-positive DCs in COVID-19 patients admitted to the ICU and non-ICU compared to the healthy control group.

Methods: The study included 10 healthy individuals and 25 COVID-19 patients. In the second week of their illness, Peripheral blood mononuclear cells (PBMCs) were isolated from the patients and labeled with targeted antibodies for HLA-DR, CD123, and CD11c. The samples were then analyzed using flow cytometry. The COVID-19 patients were divided into two ICU and non-ICU groups and were closely monitored throughout the study.

Results: In comparison to healthy controls, COVID-19 patients exhibited a significantly lower pDCs ratio (P=0.04). Patients were categorized into two groups: (A) the ICU group (n=11; 44%) and (B) the non-ICU group (n=14; 56%). The frequency of pDC was significantly lower in ICU patients than in non-ICU patients (P<0.01). Although not statistically significant, ICU patients had a lower frequency of cDCs and double positive DCs compared to non-ICU patients. Additionally, a significant association between the age of COVID-19 patients and cDC levels was observed (p=0.049).

Conclusion: SARS-CoV-2 can evade attacks from the immune response by reducing the number of DCs and suppressing their function of DCs, ultimately resulting in weakened development of both innate and adaptive immunity.

COVID-19患者树突状细胞亚群频率的评估及其与疾病严重程度的相关性
背景:COVID-19(2019)明显表现出不平衡的免疫反应。树突状细胞(dc)功能和亚型的变化可能对COVID-19患者的免疫反应产生影响,并导致免疫病理。目的:比较ICU和非ICU患者新冠肺炎患者浆细胞样树突状细胞(pDCs)、常规树突状细胞(cdc)和双阳性树突状细胞的表型和频率。方法:选取10例健康人群和25例新冠肺炎患者作为研究对象。在患者患病的第二周,从患者身上分离外周血单个核细胞(PBMCs),并标记HLA-DR、CD123和CD11c的靶向抗体。然后用流式细胞术分析样品。将COVID-19患者分为ICU组和非ICU组,在整个研究过程中密切监测。结果:与健康对照组相比,COVID-19患者的pDCs比值显著降低(P=0.04)。患者分为两组:(A) ICU组(n=11);44%)和(B)非icu组(n=14;56%)。结论:SARS-CoV-2可通过减少dc的数量和抑制dc的功能来逃避免疫应答的攻击,最终导致先天免疫和适应性免疫的发育减弱。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Iranian Journal of Immunology
Iranian Journal of Immunology Medicine-Immunology and Allergy
CiteScore
1.60
自引率
0.00%
发文量
50
审稿时长
12 weeks
期刊介绍: The Iranian Journal of Immunology (I.J.I) is an internationally disseminated peer-reviewed publication and publishes a broad range of experimental and theoretical studies concerned with all aspects of immunology.
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