Plant Omics最新文献_第2页

Agronomic performance of soybean genotypes in Brazil sub-subtropical climate 巴西亚热带气候下大豆基因型的农艺性状

Plant Omics Pub Date : 2020-05-20 DOI: 10.21475/poj.13.01.20.p1051

G. H. Demari, V. J. Szareski, I. Carvalho, V. Bubans, J. G. D. Silva, A. B. N. Martins, Leomir Morizzo, J. A. Cavalcante, E. Matter, Devid Araujo Magano, Í. Dubal, J. R. Pimentel, R. A. V. Escalera, C. E. S. Pedroso, V. Q. Souza, T. Z. Aumonde, T. Pedó

{"title":"Agronomic performance of soybean genotypes in Brazil sub-subtropical climate","authors":"G. H. Demari, V. J. Szareski, I. Carvalho, V. Bubans, J. G. D. Silva, A. B. N. Martins, Leomir Morizzo, J. A. Cavalcante, E. Matter, Devid Araujo Magano, Í. Dubal, J. R. Pimentel, R. A. V. Escalera, C. E. S. Pedroso, V. Q. Souza, T. Z. Aumonde, T. Pedó","doi":"10.21475/poj.13.01.20.p1051","DOIUrl":"https://doi.org/10.21475/poj.13.01.20.p1051","url":null,"abstract":"Soybean is the main economic crop of Brazilian agribusiness, and the extreme south of the country is considered a new agricultural frontier. The objective of this study was to evaluate the components of the yield and agronomic performance of soybean genotypes in the south of Rio Grande do Sul as well as to verify the linear relationships of the measured characters. The study was carried out in the municipality of Pelotas, Rio Grande do Sul, Brazil, in the crop harvest 2016. The experimental design was a randomized complete block design, containing 25 soybean genotypes arranged in four replicates. The experimental units were composed of five sowing lines, spaced by 0.45 meters and three meters in length. The measured characters were: first pod insertion height, plant height, number of branches, length of branches, number of pods in the main stem, number of pods in the branches, number of pods with one, two, three and four seeds, contributing to the pod mass with one, two, three and four seeds. The data were submitted to analysis of variance at 5% of probability, where for the significant characters the averages were compared by the Duncan’s test and Pearson's linear correlation was performed in order to identify the tendency of association among the measured characters. The genotypes DM 6159IPRO, BS2606 IPRO, BMX Magna, BMX Potência, TMG7062 IPRO, LG 60163 IPRO revealed superior characters, which can positively influence grain yield. There is a correlation among the morphological characters of the soybean cultivated in southern Brazil between the number of pods in the main stem and the number of pods in the branches, as well as between the number of pods with two seeds and the number of pods in the branches.","PeriodicalId":54602,"journal":{"name":"Plant Omics","volume":"1 1","pages":"1-6"},"PeriodicalIF":0.0,"publicationDate":"2020-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48219480","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Plant parasitic nematodes occurrence and genetic diversity of banana cultivars grown in Tanzania 坦桑尼亚香蕉品种植物寄生线虫的发生及遗传多样性

Plant Omics Pub Date : 2020-05-20 DOI: 10.21475/poj.13.01.20.p2085

Doreen M. Mgonja, G. Temu, S. Lyantagaye, Abdalah Makaranga, J. Ndunguru, N. Luambano

{"title":"Plant parasitic nematodes occurrence and genetic diversity of banana cultivars grown in Tanzania","authors":"Doreen M. Mgonja, G. Temu, S. Lyantagaye, Abdalah Makaranga, J. Ndunguru, N. Luambano","doi":"10.21475/poj.13.01.20.p2085","DOIUrl":"https://doi.org/10.21475/poj.13.01.20.p2085","url":null,"abstract":"The genetic diversity of bananas (Musa spp.), one of the most economically important crops in Tanzania, is underestimated with scarce reports available. In addition, cultivation of banana is severely constrained by plant parasitic nematodes (PPN). We assessed genetic diversity and population structure of 159 banana varieties from four agro-ecological zones (Northern, Southern highlands, Lake and Zanzibar islands) of Tanzania using 20 SSR markers analysed with UPGMA method. We also, assessed the PPN occurrence through isolation and counting of PPN from each banana genotype where leaf samples were collected. SSR primer pairs were polymorphic; and generated 63 distinct reproducible bands. The polymorphic information content values of each SSR marker ranged from 0.50 to 0.75 indicating high level of genetic diversity among banana varieties. The 159 banana varieties were grouped into two clusters: A and B, based on UPGMA cluster and population structure analysis. A total of 128 independent and 31 overlapping genotypes were identified. Higher kinship among genotypes was observed in cluster A compared with cluster B. Some of the clusters in A and B genotypes showed high genetic distance. The most prevalent and abundant nematode species was Pratylenchus goodeyi with a mean count of 63%, followed by Radopholus similis (31%) and P. coffeae (6%). The results from this study provides a foundation for understanding genetic variability of bananas existing in Tanzania and PPN occurrence that will be valuable information for breeding disease and pest-resistant bananas with high yield traits.","PeriodicalId":54602,"journal":{"name":"Plant Omics","volume":"1 1","pages":"21-29"},"PeriodicalIF":0.0,"publicationDate":"2020-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45417010","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Response of some wheat genotypes to different salinity levels of Irrigated water 几种小麦基因型对不同盐度灌溉水的响应

Plant Omics Pub Date : 2020-05-20 DOI: 10.21475/poj.13.01.20.p2268

Soleman M. Al Otayk

{"title":"Response of some wheat genotypes to different salinity levels of Irrigated water","authors":"Soleman M. Al Otayk","doi":"10.21475/poj.13.01.20.p2268","DOIUrl":"https://doi.org/10.21475/poj.13.01.20.p2268","url":null,"abstract":"Salinity is one of the major constraints for crop production across the world. Saudi Arabia is dominated with desert environment with high salinity in the central region of the country. Therefore, salinity has a limiting factor for cereal crops in this region. The objective of this study was to assess the productivity and quality characters for some wheat (Triticum aestivum L) genotypes under different salinity levels of Irrigated water (control, 4000, 8000 ppm NaCl). The experiment was conducted during 2011 and 2012 seasons. The trial was conducted under greenhouse environmental condition at Qassim University Agricultural Research and Experimental Station during 2011 and 2012 seasons. Twenty wheat genotypes including: 5 genotypes from ICARDA, 7 genotypes from Pakistan, 5 Australian genotypes, one American genotype (Yocora Rojo), one Egyptian genotype (Sakha 93) and one local genotype (Sama) were used in this study. Results from wheat genotype trial showed a significant difference (p<0.05) for all traits due to increased salinity in irrigation water from 4000 to 8000 ppm. There was a significant difference between the varieties for plant height, 1000-kernel weight, number of kernels spike -1 and Na for grain and straw. The interaction effect was significant in number of spikes, 1000-kernel weight and Na for grain and hay and Na / K cereal. The results showed that Auqab 2000, Bhan 2000 and Shaka 93 have the highest yield at high level of salt and Sis 13, P2 and Local were the least in yield. Moreover, the results of principle component analysis (PCA) indicated that the superior wheat genotypes for grain yield under salt stress in the two seasons (Auqab 2000, Bhan 2000, Yocara Rojo and Sakha 93) are clustered in group D. These genotypes can be considered as salinity resistant varieties. The maximum reduction over control under salt stress was recorded in Australian genotypes (P6 and P9) and local genotype 'Sama'.","PeriodicalId":54602,"journal":{"name":"Plant Omics","volume":"1 1","pages":"37-45"},"PeriodicalIF":0.0,"publicationDate":"2020-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49535741","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

NMR-based metabolomics reveals effect of Ganoderma boninense infection on oil palm leaf at 30 days post-infection 基于NMR的代谢组学揭示了感染后30天灵芝对油棕榈叶的影响

Plant Omics Pub Date : 2020-05-20 DOI: 10.21475/poj.13.01.20.p2071

A. Isha, N. Yusof, R. Osman, M. Wong, S. Abdullah

{"title":"NMR-based metabolomics reveals effect of Ganoderma boninense infection on oil palm leaf at 30 days post-infection","authors":"A. Isha, N. Yusof, R. Osman, M. Wong, S. Abdullah","doi":"10.21475/poj.13.01.20.p2071","DOIUrl":"https://doi.org/10.21475/poj.13.01.20.p2071","url":null,"abstract":"Basal stem rot is the major disease in oil palm industry that caused by a fungal named Ganoderma boninense (G. boninense) species. Infected palms are symptomless at the early stage of this disease which imposes difficulties in detecting the disease. Therefore, this study was carried out to obtain the 1 H NMR metabolomic profiling of both non-infected and G. boninense infected oil palm leaf at 30 days post-infection (dpi). This combination has provided a rapid approach in investigating the changes in the compound variations of non-infected and G. boninense infected oil palm leaf. Non-infected and G. boninense infected oil palm leaf at 30 dpi was extracted using aqueous methanol (methanol: water, 80: 20 v/v). The crude extracts obtained were analyzed by 1 H NMR-based metabolomics approach. Analysis of metabolomics data from 1 H NMR was conducted by multivariate data analysis of principal component analysis (PCA). Significant differences were found between the two groups. Compared to the non-infected leaf, the G. boninense infected leaf had higher relative abundance of choline, asparagine, alanine, succinic acid, gallic acid, epicatechin, trimethylamine, N-acetylglucosamine, N-acetyltyrosine, β-sitosterol, 2,3-butanediol, lactic acid, caffeic acid, phydroxybenzoic acid, α-tocopherol, β-cryptoxanthin and kaempferol. The non-infected leaf showed higher level of sucrose, xylose, α-glucose, S-sulfocysteine and indole-3-acetic acid. NMR-based metabolomics applied in this study reveals that G. boninense alters a manifold of primary and secondary compounds in oil palm leaf.","PeriodicalId":54602,"journal":{"name":"Plant Omics","volume":"1 1","pages":"15-20"},"PeriodicalIF":0.0,"publicationDate":"2020-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44048143","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Genome-wide identification of miRNAs targets involved in cold response in cassava 木薯冷反应中miRNA靶点的全基因组鉴定

Plant Omics Pub Date : 2020-05-20 DOI: 10.21475/poj.13.01.20.p2337

Shuxia Li, Zhihao Cheng, M. Peng

{"title":"Genome-wide identification of miRNAs targets involved in cold response in cassava","authors":"Shuxia Li, Zhihao Cheng, M. Peng","doi":"10.21475/poj.13.01.20.p2337","DOIUrl":"https://doi.org/10.21475/poj.13.01.20.p2337","url":null,"abstract":"MicroRNAs (miRNAs) are recognized as essential transcriptional or post-transcriptional regulators, and play versatile roles in plants, functioning in processes such as growth, development and stress responses. Cassava (Manihot esculenta) is a major root crop widely grown as a staple food, animal feed, and also as an important source of bioethanol worldwide. Cold stress seriously affects cassava plants growth, development and yield. MiRNAs and their targets have been extensively studied in model plants, but a genomewide identification of miRNAs’ targets is still lacking in cassava. In this study, to identify the roles of miRNAs and their targets in response to cold stress, two degradome libraries were constructed using cold-treated and non-cold-treated cassava seedlings. Degradome data allowed us to identify a total of 151 non-redundant miRNA-target pairs from the degradome data. We reveal that approximately 42% of miRNA targets are conserved across plant species. However, 83 novel miRNA targets were identified in the two libraries, suggesting a specific role for these genes in response to cold stress. Gene ontology analyses showed that many target genes involved in “cellular process” and “metabolic process”. In addition, 12 miRNAs and 31 corresponding targets of them were further found to be involved in cold stress response. Particularly, miR159, miR164 and miR396 participated in cold stress response by up-regulating certain MYBs, NACs and GRFs genes that were involved in the regulation of downstream gene expression. Meantime, three miRNAs and five target genes were validated by quantitative real-time PCR (qRT-PCR). The work helps identify cold-responsive miRNA targets in cassava and increases the number of novel targets involved in cold stress response. Furthermore, the findings of this study might provide valuable reference and new insights for understanding the functions of miRNA in stress response in plants.","PeriodicalId":54602,"journal":{"name":"Plant Omics","volume":"1 1","pages":"57-64"},"PeriodicalIF":0.0,"publicationDate":"2020-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47606205","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Path analysis and canonical variables of intervarietal maize hybrids 玉米品种间杂交种的通径分析及典型变量

Plant Omics Pub Date : 2019-12-20 DOI: 10.21475/POJ.12.01.19.PT848

M. H. Barbosa, I. Carvalho, V. J. Szareski, G. G. Conte, A. J. D. Pelegrin, M. Ferrari, T. Rosa, R. Mambrin, M. Nardino, A. Oliveira, L. C. Maia, V. Q. Souza

{"title":"Path analysis and canonical variables of intervarietal maize hybrids","authors":"M. H. Barbosa, I. Carvalho, V. J. Szareski, G. G. Conte, A. J. D. Pelegrin, M. Ferrari, T. Rosa, R. Mambrin, M. Nardino, A. Oliveira, L. C. Maia, V. Q. Souza","doi":"10.21475/POJ.12.01.19.PT848","DOIUrl":"https://doi.org/10.21475/POJ.12.01.19.PT848","url":null,"abstract":"Maize (Zea mays L.) is the cereal most produced in the world, due to its wide scope and utilization in human and animal diet. This study aims to evaluate the agronomic performance of intervarietal maize hybrids, as well as the linear associations, interrelations of cause and effect, and the genotypes dispersion through canonical variates. The experiment was conducted in the agricultural year of 2014/2015. The crosses that originated the hybrids were carried out on the growing season of 2013/2014 and hybrids evaluated on 2014/2015. The hybrids were arranged in randomized blocks, being 13 treatments with five repetitions. The measured characters were: plant height, spike insertion height, stem diameter, spike diameter, spike length, spike mass, number of rows of grains per spike, number of grains per row, cob diameter, cob mass, spike grains mass, mass of a thousand grains, gra in length and grain yield. The data were submitted to analysis of variance and mean values compared by the Tukey test at 5% of probability. The Pearson’s linear correlation analysis and path analysis were performed using grain yield as a dependent character. Furthermore, the analysis of canonical variables was carried out. The hybrid H5: G3 X G4 revealed higher grain yield, spike grains mass, number of grains per row and spike diameter. Grain yield of intervarietal hybrids presented positive correlations with the traits such as stem diameter, spike diameter, spike length, number of grains per row, mass of a thousand grains, grain length and spike grains mass. Spike diameter and spike length presented higher direct effects on grain yield of intervarietal hybrids. The canonical variates revealed the formation of five phenotypically distinct groups of intervarietal hybrids.","PeriodicalId":54602,"journal":{"name":"Plant Omics","volume":"1 1","pages":"1-8"},"PeriodicalIF":0.0,"publicationDate":"2019-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41717178","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Economic efficiency and soybean yield due to the use of different fungicide combinations 不同杀菌剂组合的经济效益和大豆产量

Plant Omics Pub Date : 2019-12-20 DOI: 10.21475/10.21475/POJ.12.01.19.PT1156

D. Knebel, Diecson Ruy Orsolin da Silva, G. H. Demari, I. Carvalho, V. J. Szareski, Lucian Alex Santos, A. J. D. Pelegrin, D. S. Muraro, M. H. Barbosa, Antonio David Bortoluzzi Silva, F. Lautenchleger, J. R. Pimentel, Cristian Troyjack, F. A. Villela, V. Q. Souza

{"title":"Economic efficiency and soybean yield due to the use of different fungicide combinations","authors":"D. Knebel, Diecson Ruy Orsolin da Silva, G. H. Demari, I. Carvalho, V. J. Szareski, Lucian Alex Santos, A. J. D. Pelegrin, D. S. Muraro, M. H. Barbosa, Antonio David Bortoluzzi Silva, F. Lautenchleger, J. R. Pimentel, Cristian Troyjack, F. A. Villela, V. Q. Souza","doi":"10.21475/10.21475/POJ.12.01.19.PT1156","DOIUrl":"https://doi.org/10.21475/10.21475/POJ.12.01.19.PT1156","url":null,"abstract":"In Brazil, Asian rust is the main disease that affects the soybean crop, which is responsible for the great inventiveness of financial resources with fungicides to control this pathogen. The objective of this work was to evaluate the grain yield and economic viability of the different combinations of fungicides used in the soybean crop. The experiment was conducted in the 2014/2015 agricultural year in the experimental field located in the municipality of Campo Novo-RS, Brazil. The treatments used correspond to 15 combinations of fungicides, and these were applied at different times during the soybean cycle. The characters measured weremass of 1000 seeds, grain yield, gross income, fungicide cost, total cost, net income, income gain and profitability. The grain yield of soybeans was reduced by 35% due to the absence of fungicide applications. Combinations of fungicides that provide the highest yields and profitability for soybean are based on the use of different active principles such asstrobilurins and carboxamides.","PeriodicalId":54602,"journal":{"name":"Plant Omics","volume":"1 1","pages":"9-14"},"PeriodicalIF":0.0,"publicationDate":"2019-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49427447","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Screening of beneficial bacteria associated with Calophyllum brasiliense Cambess so as to develop microbial inoculants for agriculture 巴西嫩叶相关有益菌的筛选，开发农业微生物接种剂

Plant Omics Pub Date : 2019-09-20 DOI: 10.21475/poj.12.02.19.p2025

Bruno Rogerio de Souza, Lílian Estrela Borges Baldotto, A. M. Paula, F. Campos, Klever Cristiano Silveira, P. M. Vieira, F. F. Sodré, J. Busato

{"title":"Screening of beneficial bacteria associated with Calophyllum brasiliense Cambess so as to develop microbial inoculants for agriculture","authors":"Bruno Rogerio de Souza, Lílian Estrela Borges Baldotto, A. M. Paula, F. Campos, Klever Cristiano Silveira, P. M. Vieira, F. F. Sodré, J. Busato","doi":"10.21475/poj.12.02.19.p2025","DOIUrl":"https://doi.org/10.21475/poj.12.02.19.p2025","url":null,"abstract":"Beneficial bacteria belong to a group of microorganisms that are able to enhance plant growth by several mechanisms. In this study, phosphorus and zinc solubilizing, nitrogen-fixing and indole acetic acid (IAA) producing bacteria associated with C. brasiliensis were screened to develop microbial inoculants (MI) for use in agriculture. Characterization and identification of screened bacteria were also performed. A total of thirty-one nitrogen-fixing bacteria isolates were obtained, of which 28 showed the ability to solubilize phosphorus and 25 to solubilize zinc. The isolate AP-JNFb-3-2, belonging to the genus Pseudomonas, presented the highest value of solubilized phosphorus (100.4 µg mL-1). The isolates AP-JNFb-3-2 and RHI-JMVL-3-1, associated with the genera Pseudomonas and Enterobacter, respectively, showed the highest values for zinc solubilization (147.8 and 147.7 µg mL-1). Isolate RO-LGI.P-3-2, belonging to the genus Pseudomonas, was the most promising for IAA production (0.52 µg mL-1). The results obtained showed the presence of a large diversity of beneficial bacteria associated with C. brasiliensis, which may be explored as MI in agriculture.","PeriodicalId":54602,"journal":{"name":"Plant Omics","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48919395","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Differential and developmental stage specific abundance of Zmdreb2a mRNA transcripts under drought stress and root development in Zea mays (L.) 干旱胁迫和根系发育条件下玉米Zmdreb2a mRNA转录本的差异及发育阶段特异性丰度

Plant Omics Pub Date : 2019-09-20 DOI: 10.21475/poj.12.02.19.p1763

Rakesh Sharma, M. Azad, V. Nunia, S. Kothari, S. Kachhwaha

{"title":"Differential and developmental stage specific abundance of Zmdreb2a mRNA transcripts under drought stress and root development in Zea mays (L.)","authors":"Rakesh Sharma, M. Azad, V. Nunia, S. Kothari, S. Kachhwaha","doi":"10.21475/poj.12.02.19.p1763","DOIUrl":"https://doi.org/10.21475/poj.12.02.19.p1763","url":null,"abstract":"Environmental stress constraints like temperature, drought and salinity affect plant growth, development and productivity negatively. In maize (Zea mays L.) water-deficiency affects flowering, pollination and embryo development. Transgenic plants having drought tolerance is a priority target in maize breeding programs worldwide. Various transcription factors play a key role in plant development and stress management. DRE-Binding Protein 2A (DREB2A) has been shown to play a central role in drought tolerance in Arabidopsis thaliana. The homologue of dreb2a in Zea mays is Zmdreb2a, a well-known transcription factor regulating genes induced under stress conditions. Studies indicated that Zmdreb2a transgenic plants show not only stress tolerance but also growth retardation. It is a major targeted gene for transgenesis to produce drought-tolerant plants. However, to make construct for transgenic plants, there is need to consider functional transcripts and regulating mechanism of Zmdreb2a mRNAs under drought condition. We have analyzed publicly available RNASeq data of leaf meristem, ovary and developing root and used a host of FastQC, Tophat, Cufflinks, Cuffmerge, Cuffdiff and ‘R’ packages to functionally characterize the differentially expressed genes (DEGs). In addition, prediction of protein structure, nuclear localization signals and transactivation domain were performed using cNLS mapper and 9aaTAD prediction tools. Alternative splicing of Zmdreb2a pre-mRNA was shown to be strongly associated with developmental stage and isoforms show fluctuating expression under stress. This study provides putative functional transcripts of Zmdreb2a, and NMD as a regulating mechanism to control their abundance.","PeriodicalId":54602,"journal":{"name":"Plant Omics","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48504831","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Expression analysis of key enzymes involved in the accumulation of iridoid in Rehmannia glutinosa 地黄环烯醚酮积累关键酶的表达分析

Plant Omics Pub Date : 2019-09-20 DOI: 10.21475/poj.12.02.19.p2221

H. Duan, Wenxiao Liu, Yunpeng Zeng, W. Jia, Huihui Wang, Yanqing Zhou

{"title":"Expression analysis of key enzymes involved in the accumulation of iridoid in Rehmannia glutinosa","authors":"H. Duan, Wenxiao Liu, Yunpeng Zeng, W. Jia, Huihui Wang, Yanqing Zhou","doi":"10.21475/poj.12.02.19.p2221","DOIUrl":"https://doi.org/10.21475/poj.12.02.19.p2221","url":null,"abstract":"As the traditional Chinese herb, Rehmannia glutinosa (R. glutinosa) has significant effects on health. The main active ingredient of R. glutinosa is iridoid. In this study, root, stem and leaf of R. glutinosa at five different growth stages were collected, the content of iridoid in R. glutinosa was determinated, and the expression of key enzymes in R. glutinosa was analyzed by quantitative real-time PCR (q-PCR). We found that the content of iridoid was increased continuously during the first three growth stages (I-III) of R. glutinosa. It reached the maximum value in root and leaf at growth stage III of R. glutinosa. However, the content of iridoid was not lower in leaf, compared with root. 1-deoxy-D-xylulose-5-phosphate reductoisomerase (DXR), Geranyl pyrophosphate synthase (GPPS), Geraniol 10-hydroxylase (G10H) and 10-hydroxygeraniol oxidoreductase (10HGO) were the key enzymes involved in the synthesis of iridoid in plant, but their expression pattern or level was different in various tissues and stages of R. glutinosa. All appeared tissue specificity and their expression was related to the accumulation of iridoid in R. glutinosa. Thus, DXR, GPPS, G10H and 10HGO might take part in the synthesis of iridoid in R. glutinosa and could be differently regulated during the growth and development of R. glutinosa, which would provide theoretical basis for the research on secondary metabolism of iridoid in R. glutinosa.","PeriodicalId":54602,"journal":{"name":"Plant Omics","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46223612","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5