CRISPR Journal最新文献_第6页

Generation of a Commercial-Scale Founder Population of Porcine Reproductive and Respiratory Syndrome Virus Resistant Pigs Using CRISPR-Cas. 利用 CRISPR-Cas 生成猪繁殖与呼吸综合征病毒抗性猪的商业规模创始人种群。

IF 3.7 4区生物学

CRISPR Journal Pub Date : 2024-02-01 DOI: 10.1089/crispr.2023.0061

Brian T Burger, Benjamin P Beaton, Matthew A Campbell, Benjamin T Brett, Melissa S Rohrer, Sarah Plummer, Dylan Barnes, Ke Jiang, Sudhir Naswa, Jeremy Lange, Alina Ott, Elizabeth Alger, Gonzalo Rincon, Steven Rounsley, Jeff Betthauser, Namdori R Mtango, Joshua A Benne, Jessica Hammerand, Codie J Durfee, Marisa L Rotolo, Peter Cameron, Alexandra M Lied, Matthew J Irby, David B Nyer, Chris K Fuller, Scott Gradia, Steven B Kanner, Ki-Eun Park, Jerel Waters, Sean Simpson, Bhanu P Telugu, Brianna C Salgado, Alberto Brandariz-Nuñez, Raymond R R Rowland, Matt Culbertson, Elena Rice, A Mark Cigan

{"title":"Generation of a Commercial-Scale Founder Population of Porcine Reproductive and Respiratory Syndrome Virus Resistant Pigs Using CRISPR-Cas.","authors":"Brian T Burger, Benjamin P Beaton, Matthew A Campbell, Benjamin T Brett, Melissa S Rohrer, Sarah Plummer, Dylan Barnes, Ke Jiang, Sudhir Naswa, Jeremy Lange, Alina Ott, Elizabeth Alger, Gonzalo Rincon, Steven Rounsley, Jeff Betthauser, Namdori R Mtango, Joshua A Benne, Jessica Hammerand, Codie J Durfee, Marisa L Rotolo, Peter Cameron, Alexandra M Lied, Matthew J Irby, David B Nyer, Chris K Fuller, Scott Gradia, Steven B Kanner, Ki-Eun Park, Jerel Waters, Sean Simpson, Bhanu P Telugu, Brianna C Salgado, Alberto Brandariz-Nuñez, Raymond R R Rowland, Matt Culbertson, Elena Rice, A Mark Cigan","doi":"10.1089/crispr.2023.0061","DOIUrl":"10.1089/crispr.2023.0061","url":null,"abstract":"Disease resistance genes in livestock provide health benefits to animals and opportunities for farmers to meet the growing demand for affordable, high-quality protein. Previously, researchers used gene editing to modify the porcine CD163 gene and demonstrated resistance to a harmful virus that causes porcine reproductive and respiratory syndrome (PRRS). To maximize potential benefits, this disease resistance trait needs to be present in commercially relevant breeding populations for multiplication and distribution of pigs. Toward this goal, a first-of-its-kind, scaled gene editing program was established to introduce a single modified CD163 allele into four genetically diverse, elite porcine lines. This effort produced healthy pigs that resisted PRRS virus infection as determined by macrophage and animal challenges. This founder population will be used for additional disease and trait testing, multiplication, and commercial distribution upon regulatory approval. Applying CRISPR-Cas to eliminate a viral disease represents a major step toward improving animal health.","PeriodicalId":54232,"journal":{"name":"CRISPR Journal","volume":"7 1","pages":"12-28"},"PeriodicalIF":3.7,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139731029","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

"CRISPR" Mutations: Inaccurate Linguistic Variations and Misrepresentation of the CRISPR Acronym. "CRISPR "突变：不准确的语言变化和对 CRISPR 首字母缩写词的错误表述。

IF 3.7 4区生物学

CRISPR Journal Pub Date : 2024-02-01 DOI: 10.1089/crispr.2024.0005

Jaime A Teixeira da

引用次数: 0

Generation of Human Isogenic Induced Pluripotent Stem Cell Lines with CRISPR Prime Editing. 利用 CRISPR 基因编辑技术生成人类同源诱导多能干细胞系。

IF 3.7 4区生物学

CRISPR Journal Pub Date : 2024-02-01 DOI: 10.1089/crispr.2023.0066

Lori L Bonnycastle, Amy J Swift, Erin C Mansell, Angela Lee, Elizabeth Winnicki, Elizabeth S Li, Catherine C Robertson, Victoria A Parsons, Trung Huynh, Chad Krilow, Karen L Mohlke, Michael R Erdos, Narisu Narisu, Francis S Collins

{"title":"Generation of Human Isogenic Induced Pluripotent Stem Cell Lines with CRISPR Prime Editing.","authors":"Lori L Bonnycastle, Amy J Swift, Erin C Mansell, Angela Lee, Elizabeth Winnicki, Elizabeth S Li, Catherine C Robertson, Victoria A Parsons, Trung Huynh, Chad Krilow, Karen L Mohlke, Michael R Erdos, Narisu Narisu, Francis S Collins","doi":"10.1089/crispr.2023.0066","DOIUrl":"10.1089/crispr.2023.0066","url":null,"abstract":"We developed an efficient CRISPR prime editing protocol and generated isogenic-induced pluripotent stem cell (iPSC) lines carrying heterozygous or homozygous alleles for putatively causal single nucleotide variants at six type 2 diabetes loci (ABCC8, MTNR1B, TCF7L2, HNF4A, CAMK1D, and GCK). Our two-step sequence-based approach to first identify transfected cell pools with the highest fraction of edited cells significantly reduced the downstream efforts to isolate single clones of edited cells. We found that prime editing can make targeted genetic changes in iPSC and optimization of system components and guide RNA designs that were critical to achieve acceptable efficiency. Systems utilizing PEmax, epegRNA modifications, and MLH1dn provided significant benefit, producing editing efficiencies of 36-73%. Editing success and pegRNA design optimization required for each variant differed depending on the sequence at the target site. With attention to design, prime editing is a promising approach to generate isogenic iPSC lines, enabling the study of specific genetic changes in a common genetic background.","PeriodicalId":54232,"journal":{"name":"CRISPR Journal","volume":"7 1","pages":"53-67"},"PeriodicalIF":3.7,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10880268/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139731030","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Whole-Genome Sequencing Reveals Rare Off-Target Mutations in MC1R-Edited Pigs Generated by Using CRISPR-Cas9 and Somatic Cell Nuclear Transfer. 全基因组测序揭示了利用 CRISPR-Cas9 和体细胞核移植技术生成的 MC1R 编辑猪的罕见脱靶突变。

IF 3.7 4区生物学

CRISPR Journal Pub Date : 2024-02-01 DOI: 10.1089/crispr.2023.0034

Zhenyang Li, Jin Lan, Xuan Shi, Tong Lu, Xiaoli Hu, Xiaohong Liu, Yaosheng Chen, Zuyong He

引用次数: 0

Easy-to-Use CRISPR-Cas9 Genome Editing in the Cultured Pacific Abalone (Haliotis discus hannai). 在养殖的太平洋鲍鱼（Haliotis discus hannai）中进行易于使用的 CRISPR-Cas9 基因组编辑。

IF 3.7 4区生物学

CRISPR Journal Pub Date : 2024-02-01 DOI: 10.1089/crispr.2023.0070

Ruohui Li, Yue Xu, Fucun Wu, Zhangjie Peng, Jiulin Chan, Linlin Zhang

{"title":"Easy-to-Use CRISPR-Cas9 Genome Editing in the Cultured Pacific Abalone (Haliotis discus hannai).","authors":"Ruohui Li, Yue Xu, Fucun Wu, Zhangjie Peng, Jiulin Chan, Linlin Zhang","doi":"10.1089/crispr.2023.0070","DOIUrl":"10.1089/crispr.2023.0070","url":null,"abstract":"The Pacific abalone is an important aquaculture shellfish and serves as an important model in basic biology study. However, the study of abalone is limited by lack of highly efficient and easy-to-use gene-editing tools. In this paper, we demonstrate efficient gene knockout in Pacific abalone using CRISPR-Cas9. We developed a highly effective microinjection method by nesting fertilized eggs in a low-concentration agarose gel. We identified the cilia developmental gene β-tubulin and light-sensitive transmembrane protein r-opsin as target genes and designed highly specific sgRNAs for modifying their genomic sequences. Sanger sequencing of the genomic regions of β-tubulin and r-opsin genes from injected larvae identified various genomic long-fragment deletions. In situ hybridization showed gene expression patterns of β-tubulin and r-opsin were significantly altered in the mosaic mutants. Knocking out β-tubulin in abalone embryos efficiently affected cilia development. Scanning electron microscopy and swimming behavior assay showed defecting cilia and decreased motility. Moreover, knocking out of r-opsin in abalone embryos effectively affected the expression and development of eyespots. Overall, this work developed an easy-to-use mosaic gene knockout protocol for abalone, which will allow researchers to utilize CRISPR-Cas9 approaches to study unexploited abalone biology and will lead to novel breeding methods for this aquaculture species.","PeriodicalId":54232,"journal":{"name":"CRISPR Journal","volume":"7 1","pages":"41-52"},"PeriodicalIF":3.7,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139731028","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Germline Editing of Drosophila Using CRISPR-Cas9-Based Cytosine and Adenine Base Editors. 使用基于CRISPR-Cas9的胞嘧啶和腺嘌呤碱基编辑器编辑果蝇的种系。

IF 3.7 4区生物学

CRISPR Journal Pub Date : 2023-12-01 Epub Date: 2023-11-02 DOI: 10.1089/crispr.2023.0026

Nirav Thakkar, Adela Hejzlarova, Vaclav Brabec, David Dolezel

引用次数: 0

Correction to: Genotyping MUltiplexed-Sequencing of CRISPR-Localized Editing (GMUSCLE): An Experimental and Computational Approach for Analyzing CRISPR-Edited Cells by Zhang et al. The CRISPR Journal, 2023;6(5):462-472; DOI: 10.1089/crispr.2023.0021. 更正：CRISPR定位编辑的基因分型多重测序（GMUSCLE）：张等人分析CRISPR编辑细胞的实验和计算方法。CRISPR期刊，2023；6（5）:462-472；DOI:10.1089/crispr.2023.0021。

IF 3.7 4区生物学

CRISPR Journal Pub Date : 2023-12-01 Epub Date: 2023-11-08 DOI: 10.1089/crispr.2023.0021.correx

引用次数: 0

Special Issue: CRISPR Trials. 特刊：CRISPR 试验。

IF 3.7 4区生物学

CRISPR Journal Pub Date : 2023-12-01 DOI: 10.1089/crispr.2023.29166.cfp2

Fyodor Urnov

引用次数: 0

The Expanding Dissemination and Distribution Patterns of Diverse CRISPR Plasmids by Addgene. 利用Addgene扩大CRISPR质粒的传播和分布模式。

IF 3.7 4区生物学

CRISPR Journal Pub Date : 2023-12-01 Epub Date: 2023-11-22 DOI: 10.1089/crispr.2023.0059

Brook Pyhtila, Seth Kasowitz, Rachel Leeson, Rodolphe Barrangou

{"title":"The Expanding Dissemination and Distribution Patterns of Diverse CRISPR Plasmids by Addgene.","authors":"Brook Pyhtila, Seth Kasowitz, Rachel Leeson, Rodolphe Barrangou","doi":"10.1089/crispr.2023.0059","DOIUrl":"10.1089/crispr.2023.0059","url":null,"abstract":"CRISPR-based technologies have rapidly enabled the democratization of genome editing in academic institutions through distribution by Addgene over the past decade. Recently, several distribution milestones have been reached, with a collection of >15,000 plasmids deposited by >1,000 laboratories spanning ∼40 countries now shipped 300,000 times to ∼5,000 organizations traversing ∼100 countries. Yet, both deposits of and requests for CRISPR plasmids continue to rise for this disruptive technology. Distribution patterns revealed robust demand for three distinct classes of CRISPR effectors, namely nucleases (e.g., Cas9 and Cas12), modulators (deactivated CRISPR nucleases fused to transcriptional regulators and epigenome modifiers), and chimeric effectors (Cas proteins fused to enzymes carrying out other activities such as deamination, reverse transcription, transposition, and integration). Yearly deposits over the past decade are requested in near-even proportions, reflecting continuous technological development and requests for novel constructs. Though it is unclear whether the slowing rate of requests is inherent to a pandemic operational lag or a transition from emerging to mature technology, it is noteworthy that the relative proportion of requests from plasmids deposited in the previous year remains stable, suggesting robust development of novel tools concurrent with continued adoption of editing, base editing, prime editing, and more. Predictably, most requested plasmids are designed for mammalian genome manipulation, presumably for medical research and human health pursuits, reflecting investments in therapeutic applications. Concurrently, requests for plant and microbial constructs are on the rise, especially in regions of the world more reliant on local agricultural inputs and focused on food and feed applications, illustrating continued diversification of genome editing applications.","PeriodicalId":54232,"journal":{"name":"CRISPR Journal","volume":" ","pages":"493-501"},"PeriodicalIF":3.7,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10753985/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138447030","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Engineered Antiviral Sensor Targets Infected Mosquitoes. 工程抗病毒传感器锁定受感染的蚊子

IF 3.7 4区生物学

CRISPR Journal Pub Date : 2023-12-01 DOI: 10.1089/crispr.2023.0056

Elena Dalla Benetta, Adam J López-Denman, Hsing-Han Li, Reem A Masri, Daniel J Brogan, Michelle Bui, Ting Yang, Ming Li, Michael Dunn, Melissa J Klein, Sarah Jackson, Kyle Catalan, Kim R Blasdell, Priscilla Tng, Igor Antoshechkin, Luke S Alphey, Prasad N Paradkar, Omar S Akbari

{"title":"Engineered Antiviral Sensor Targets Infected Mosquitoes.","authors":"Elena Dalla Benetta, Adam J López-Denman, Hsing-Han Li, Reem A Masri, Daniel J Brogan, Michelle Bui, Ting Yang, Ming Li, Michael Dunn, Melissa J Klein, Sarah Jackson, Kyle Catalan, Kim R Blasdell, Priscilla Tng, Igor Antoshechkin, Luke S Alphey, Prasad N Paradkar, Omar S Akbari","doi":"10.1089/crispr.2023.0056","DOIUrl":"10.1089/crispr.2023.0056","url":null,"abstract":"Escalating vector disease burdens pose significant global health risks, as such innovative tools for targeting mosquitoes are critical. CRISPR-Cas technologies have played a crucial role in developing powerful tools for genome manipulation in various eukaryotic organisms. Although considerable efforts have focused on utilizing class II type II CRISPR-Cas9 systems for DNA targeting, these modalities are unable to target RNA molecules, limiting their utility against RNA viruses. Recently, the Cas13 family has emerged as an efficient tool for RNA targeting; however, the application of this technique in mosquitoes, particularly Aedes aegypti, has yet to be fully realized. In this study, we engineered an antiviral strategy termed REAPER (vRNA Expression Activates Poisonous Effector Ribonuclease) that leverages the programmable RNA-targeting capabilities of CRISPR-Cas13 and its potent collateral activity. REAPER remains concealed within the mosquito until an infectious blood meal is uptaken. Upon target viral RNA infection, REAPER activates, triggering programmed destruction of its target arbovirus such as chikungunya. Consequently, Cas13-mediated RNA targeting significantly reduces viral replication and viral prevalence of infection, and its promiscuous collateral activity can even kill infected mosquitoes within a few days. This innovative REAPER technology adds to an arsenal of effective molecular genetic tools to combat mosquito virus transmission.","PeriodicalId":54232,"journal":{"name":"CRISPR Journal","volume":"6 6","pages":"543-556"},"PeriodicalIF":3.7,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11085028/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138807971","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0