CRISPR Journal最新文献_第4页

CRISPR Momentum in the Clinic and the Field. CRISPR 在临床和科研领域的发展势头。

IF 3.7 4区生物学

CRISPR Journal Pub Date : 2024-02-01 DOI: 10.1089/crispr.2024.29172.editorial

Rodolphe Barrangou, Kevin Davies

引用次数: 0

Warrior Spirit: An Interview with Victoria Gray, Sickle Cell Pioneer. 勇士精神：镰状细胞先驱维多利亚-格雷访谈录。

IF 3.7 4区生物学

CRISPR Journal Pub Date : 2024-02-01 DOI: 10.1089/crispr.2024.29171.vgr

Victoria Gray, Uduak Thomas, Kevin Davies

引用次数: 0

Generation of a Commercial-Scale Founder Population of Porcine Reproductive and Respiratory Syndrome Virus Resistant Pigs Using CRISPR-Cas. 利用 CRISPR-Cas 生成猪繁殖与呼吸综合征病毒抗性猪的商业规模创始人种群。

IF 3.7 4区生物学

CRISPR Journal Pub Date : 2024-02-01 DOI: 10.1089/crispr.2023.0061

Brian T Burger, Benjamin P Beaton, Matthew A Campbell, Benjamin T Brett, Melissa S Rohrer, Sarah Plummer, Dylan Barnes, Ke Jiang, Sudhir Naswa, Jeremy Lange, Alina Ott, Elizabeth Alger, Gonzalo Rincon, Steven Rounsley, Jeff Betthauser, Namdori R Mtango, Joshua A Benne, Jessica Hammerand, Codie J Durfee, Marisa L Rotolo, Peter Cameron, Alexandra M Lied, Matthew J Irby, David B Nyer, Chris K Fuller, Scott Gradia, Steven B Kanner, Ki-Eun Park, Jerel Waters, Sean Simpson, Bhanu P Telugu, Brianna C Salgado, Alberto Brandariz-Nuñez, Raymond R R Rowland, Matt Culbertson, Elena Rice, A Mark Cigan

{"title":"Generation of a Commercial-Scale Founder Population of Porcine Reproductive and Respiratory Syndrome Virus Resistant Pigs Using CRISPR-Cas.","authors":"Brian T Burger, Benjamin P Beaton, Matthew A Campbell, Benjamin T Brett, Melissa S Rohrer, Sarah Plummer, Dylan Barnes, Ke Jiang, Sudhir Naswa, Jeremy Lange, Alina Ott, Elizabeth Alger, Gonzalo Rincon, Steven Rounsley, Jeff Betthauser, Namdori R Mtango, Joshua A Benne, Jessica Hammerand, Codie J Durfee, Marisa L Rotolo, Peter Cameron, Alexandra M Lied, Matthew J Irby, David B Nyer, Chris K Fuller, Scott Gradia, Steven B Kanner, Ki-Eun Park, Jerel Waters, Sean Simpson, Bhanu P Telugu, Brianna C Salgado, Alberto Brandariz-Nuñez, Raymond R R Rowland, Matt Culbertson, Elena Rice, A Mark Cigan","doi":"10.1089/crispr.2023.0061","DOIUrl":"10.1089/crispr.2023.0061","url":null,"abstract":"Disease resistance genes in livestock provide health benefits to animals and opportunities for farmers to meet the growing demand for affordable, high-quality protein. Previously, researchers used gene editing to modify the porcine CD163 gene and demonstrated resistance to a harmful virus that causes porcine reproductive and respiratory syndrome (PRRS). To maximize potential benefits, this disease resistance trait needs to be present in commercially relevant breeding populations for multiplication and distribution of pigs. Toward this goal, a first-of-its-kind, scaled gene editing program was established to introduce a single modified CD163 allele into four genetically diverse, elite porcine lines. This effort produced healthy pigs that resisted PRRS virus infection as determined by macrophage and animal challenges. This founder population will be used for additional disease and trait testing, multiplication, and commercial distribution upon regulatory approval. Applying CRISPR-Cas to eliminate a viral disease represents a major step toward improving animal health.","PeriodicalId":54232,"journal":{"name":"CRISPR Journal","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139731029","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

"CRISPR" Mutations: Inaccurate Linguistic Variations and Misrepresentation of the CRISPR Acronym. "CRISPR "突变：不准确的语言变化和对 CRISPR 首字母缩写词的错误表述。

IF 3.7 4区生物学

CRISPR Journal Pub Date : 2024-02-01 DOI: 10.1089/crispr.2024.0005

Jaime A Teixeira da

引用次数: 0

Generation of Human Isogenic Induced Pluripotent Stem Cell Lines with CRISPR Prime Editing. 利用 CRISPR 基因编辑技术生成人类同源诱导多能干细胞系。

IF 3.7 4区生物学

CRISPR Journal Pub Date : 2024-02-01 DOI: 10.1089/crispr.2023.0066

Lori L Bonnycastle, Amy J Swift, Erin C Mansell, Angela Lee, Elizabeth Winnicki, Elizabeth S Li, Catherine C Robertson, Victoria A Parsons, Trung Huynh, Chad Krilow, Karen L Mohlke, Michael R Erdos, Narisu Narisu, Francis S Collins

{"title":"Generation of Human Isogenic Induced Pluripotent Stem Cell Lines with CRISPR Prime Editing.","authors":"Lori L Bonnycastle, Amy J Swift, Erin C Mansell, Angela Lee, Elizabeth Winnicki, Elizabeth S Li, Catherine C Robertson, Victoria A Parsons, Trung Huynh, Chad Krilow, Karen L Mohlke, Michael R Erdos, Narisu Narisu, Francis S Collins","doi":"10.1089/crispr.2023.0066","DOIUrl":"10.1089/crispr.2023.0066","url":null,"abstract":"We developed an efficient CRISPR prime editing protocol and generated isogenic-induced pluripotent stem cell (iPSC) lines carrying heterozygous or homozygous alleles for putatively causal single nucleotide variants at six type 2 diabetes loci (ABCC8, MTNR1B, TCF7L2, HNF4A, CAMK1D, and GCK). Our two-step sequence-based approach to first identify transfected cell pools with the highest fraction of edited cells significantly reduced the downstream efforts to isolate single clones of edited cells. We found that prime editing can make targeted genetic changes in iPSC and optimization of system components and guide RNA designs that were critical to achieve acceptable efficiency. Systems utilizing PEmax, epegRNA modifications, and MLH1dn provided significant benefit, producing editing efficiencies of 36-73%. Editing success and pegRNA design optimization required for each variant differed depending on the sequence at the target site. With attention to design, prime editing is a promising approach to generate isogenic iPSC lines, enabling the study of specific genetic changes in a common genetic background.","PeriodicalId":54232,"journal":{"name":"CRISPR Journal","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10880268/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139731030","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Whole-Genome Sequencing Reveals Rare Off-Target Mutations in MC1R-Edited Pigs Generated by Using CRISPR-Cas9 and Somatic Cell Nuclear Transfer. 全基因组测序揭示了利用 CRISPR-Cas9 和体细胞核移植技术生成的 MC1R 编辑猪的罕见脱靶突变。

IF 3.7 4区生物学

CRISPR Journal Pub Date : 2024-02-01 DOI: 10.1089/crispr.2023.0034

Zhenyang Li, Jin Lan, Xuan Shi, Tong Lu, Xiaoli Hu, Xiaohong Liu, Yaosheng Chen, Zuyong He

引用次数: 0

Easy-to-Use CRISPR-Cas9 Genome Editing in the Cultured Pacific Abalone (Haliotis discus hannai). 在养殖的太平洋鲍鱼（Haliotis discus hannai）中进行易于使用的 CRISPR-Cas9 基因组编辑。

IF 3.7 4区生物学

CRISPR Journal Pub Date : 2024-02-01 DOI: 10.1089/crispr.2023.0070

Ruohui Li, Yue Xu, Fucun Wu, Zhangjie Peng, Jiulin Chan, Linlin Zhang

{"title":"Easy-to-Use CRISPR-Cas9 Genome Editing in the Cultured Pacific Abalone (Haliotis discus hannai).","authors":"Ruohui Li, Yue Xu, Fucun Wu, Zhangjie Peng, Jiulin Chan, Linlin Zhang","doi":"10.1089/crispr.2023.0070","DOIUrl":"10.1089/crispr.2023.0070","url":null,"abstract":"The Pacific abalone is an important aquaculture shellfish and serves as an important model in basic biology study. However, the study of abalone is limited by lack of highly efficient and easy-to-use gene-editing tools. In this paper, we demonstrate efficient gene knockout in Pacific abalone using CRISPR-Cas9. We developed a highly effective microinjection method by nesting fertilized eggs in a low-concentration agarose gel. We identified the cilia developmental gene β-tubulin and light-sensitive transmembrane protein r-opsin as target genes and designed highly specific sgRNAs for modifying their genomic sequences. Sanger sequencing of the genomic regions of β-tubulin and r-opsin genes from injected larvae identified various genomic long-fragment deletions. In situ hybridization showed gene expression patterns of β-tubulin and r-opsin were significantly altered in the mosaic mutants. Knocking out β-tubulin in abalone embryos efficiently affected cilia development. Scanning electron microscopy and swimming behavior assay showed defecting cilia and decreased motility. Moreover, knocking out of r-opsin in abalone embryos effectively affected the expression and development of eyespots. Overall, this work developed an easy-to-use mosaic gene knockout protocol for abalone, which will allow researchers to utilize CRISPR-Cas9 approaches to study unexploited abalone biology and will lead to novel breeding methods for this aquaculture species.","PeriodicalId":54232,"journal":{"name":"CRISPR Journal","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139731028","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Benchmark Software and Data for Evaluating CRISPR-Cas9 Experimental Pipelines Through the Assessment of a Calibration Screen. 通过校准筛选评估 CRISPR-Cas9 实验流程的基准软件和数据。

IF 3.7 4区生物学

CRISPR Journal Pub Date : 2024-01-02 DOI: 10.1089/crispr.2023.0040

Raffaele M Iannuzzi, Ichcha Manipur, Clare Pacini, Fiona M Behan, Mario R Guarracino, Mathew J Garnett, Aurora Savino, Francesco Iorio

{"title":"Benchmark Software and Data for Evaluating CRISPR-Cas9 Experimental Pipelines Through the Assessment of a Calibration Screen.","authors":"Raffaele M Iannuzzi, Ichcha Manipur, Clare Pacini, Fiona M Behan, Mario R Guarracino, Mathew J Garnett, Aurora Savino, Francesco Iorio","doi":"10.1089/crispr.2023.0040","DOIUrl":"10.1089/crispr.2023.0040","url":null,"abstract":"Genome-wide genetic screens using CRISPR-guide RNA libraries are widely performed in mammalian cells to functionally characterize individual genes and for the discovery of new anticancer therapeutic targets. As the effectiveness of such powerful and precise tools for cancer pharmacogenomics is emerging, tools and methods for their quality assessment are becoming increasingly necessary. Here, we provide an R package and a high-quality reference data set for the assessment of novel experimental pipelines through which a single calibration experiment has been executed: a screen of the HT-29 human colorectal cancer cell line with a commercially available genome-wide library of single-guide RNAs. This package and data allow experimental researchers to benchmark their screens and produce a quality-control report, encompassing several quality and validation metrics. The R code used for processing the reference data set, for its quality assessment, as well as to evaluate the quality of a user-provided screen, and to reproduce the figures presented in this article is available at https://github.com/DepMap-Analytics/HT29benchmark. The reference data is publicly available on FigShare.","PeriodicalId":54232,"journal":{"name":"CRISPR Journal","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2024-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139075824","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Germline Editing of Drosophila Using CRISPR-Cas9-Based Cytosine and Adenine Base Editors. 使用基于CRISPR-Cas9的胞嘧啶和腺嘌呤碱基编辑器编辑果蝇的种系。

IF 3.7 4区生物学

CRISPR Journal Pub Date : 2023-12-01 Epub Date: 2023-11-02 DOI: 10.1089/crispr.2023.0026

Nirav Thakkar, Adela Hejzlarova, Vaclav Brabec, David Dolezel

引用次数: 0

Correction to: Genotyping MUltiplexed-Sequencing of CRISPR-Localized Editing (GMUSCLE): An Experimental and Computational Approach for Analyzing CRISPR-Edited Cells by Zhang et al. The CRISPR Journal, 2023;6(5):462-472; DOI: 10.1089/crispr.2023.0021. 更正：CRISPR定位编辑的基因分型多重测序（GMUSCLE）：张等人分析CRISPR编辑细胞的实验和计算方法。CRISPR期刊，2023；6（5）:462-472；DOI:10.1089/crispr.2023.0021。

IF 3.7 4区生物学

CRISPR Journal Pub Date : 2023-12-01 Epub Date: 2023-11-08 DOI: 10.1089/crispr.2023.0021.correx

引用次数: 0