Annals of Agricultural Science最新文献

{"title":"Exogenous γ-aminobutyric acid enhances heat tolerance of kiwifruit plants by protecting photosynthetic system and promoting heat shock proteins expression","authors":"Liuqing Huo ,&nbsp;Yunyun Chen ,&nbsp;Yuman Zhang,&nbsp;Hao Zhang,&nbsp;Hujing Wang,&nbsp;Kai Xu,&nbsp;Xuepeng Sun","doi":"10.1016/j.aoas.2023.12.003","DOIUrl":"https://doi.org/10.1016/j.aoas.2023.12.003","url":null,"abstract":"<div><p>Kiwifruit plants are highly susceptible to increased atmospheric temperature, and the extreme high temperature often causes the loss of production and quality of the fruit. The γ-aminobutyric acid (GABA) has been widely proved to play important roles in plants in response to abiotic stresses, showing an important potential for application in agricultural industry to overcome environmental challenges. However, application of GABA in kiwifruit plants to resist external stresses has not been reported yet. This study found that pre-irrigation of kiwifruit plants with 0.5 mM GABA was effective in alleviating the heat damage on them. GABA treatment led to better antioxidant capacity and reduced ROS production in kiwifruit plants under high temperature. Moreover, exogenous GABA protected the photosynthetic system of kiwifruit plants when exposed to high temperature. Particularly, we found that endogenous ABA, Glu, Pro metabolisms were participated in the GABA-mediated heat resistance of kiwifruit plants. Furthermore, GABA treatment induced higher expression of <em>AdHsps</em> in kiwifruit plants, being partly responsible for their better performance under heat stress. In summary, this research first demonstrated that exogenous GABA treatment plays a positive role in kiwifruit plants to response to extreme heat stress.</p></div>","PeriodicalId":54198,"journal":{"name":"Annals of Agricultural Science","volume":"68 2","pages":"Pages 137-147"},"PeriodicalIF":5.9,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0570178323000301/pdfft?md5=f0b83b67236591d50bfc08f693cc2ed6&pid=1-s2.0-S0570178323000301-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138577815","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Long-term fermented organic fertilizer application reduce urea nitrogen-15 loss from plastic shed agricultural soils","authors":"Hou Maomao ,&nbsp;Tang Songyan ,&nbsp;Zhu Qinyuan ,&nbsp;Chen Jingnan ,&nbsp;Xiao Ying ,&nbsp;Jin Qiu ,&nbsp;Zhong Fenglin","doi":"10.1016/j.aoas.2023.11.002","DOIUrl":"https://doi.org/10.1016/j.aoas.2023.11.002","url":null,"abstract":"<div><p>Continuous application of fermented organic fertilizer can improve soil quality, while the performance of nitrogen (N) in the improved soils is rarely investigated. To investigate the fate of applied N in the soils with organic management history, the ¹⁵NH₂CO¹⁵NH₂ (¹⁵N abundance of 19.6 %) was employed as the exogenous N source to conduct an experiment in the Chinese cabbage and tomato rotation system under plastic shed condition. The cultivated soils have received 15-year of effective microorganism (EM) fermented organic fertilizer (EM-OF), N, P, K inorganic fertilizer (NPK-IF) and no fertilizer (No<img>F). The ¹⁵N use by cabbage and tomato, the soil ¹⁵N forms, as well as the ¹⁵N distribution were observed. Results showed that the ¹⁵N use efficiency of cabbage in the EM-OF, NPK-IF and No<img>F soils were 55.1 %, 37.3 % and 26.6 % respectively, showing significant (<em>p</em> ≤ 0.05) differences. The succeeding crop tomato could take up the soil residual ¹⁵N, and the highest ¹⁵N reuse efficiency was 7.1 % that detected in the No<img>F soil. The total ¹⁵N loss (6.0 %) from the rotation system was the lowest in the EM-OF soil, compared to that in the NPK-IF and No<img>F soils. It was concluded that the long-term fermented organic fertilizer applied soils can reduce urea ¹⁵N loss from plastic shed agriculture, mainly through improving the in-season crop ¹⁵N use efficiency.</p></div>","PeriodicalId":54198,"journal":{"name":"Annals of Agricultural Science","volume":"68 2","pages":"Pages 108-117"},"PeriodicalIF":5.9,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S057017832300026X/pdfft?md5=1a200c69d441a55a459b110ea9eb25fc&pid=1-s2.0-S057017832300026X-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138472658","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Regulation of litter size in sheep (Ovis aries) by the GDF9 and BMP15 genes","authors":"Yang Chen ,&nbsp;Xuesong Shan ,&nbsp;Huaizhi Jiang ,&nbsp;Limin Sun ,&nbsp;Zhenhua Guo","doi":"10.1016/j.aoas.2023.12.004","DOIUrl":"10.1016/j.aoas.2023.12.004","url":null,"abstract":"<div><p>The mutation types <em>GDF9</em> S395F and S427R increase the ovulation rate in heterozygotes, while the homozygous form leads to infertility. This study presents a meta-analysis of <em>GDF9</em> and <em>BMP15</em> SNPs related to sheep litter size (LS). The results showed that simulation of the molecular recognition of <em>GDF9</em> R453H, S395F, and S427R was performed to study the effect on the GDF9 dimer. S395F and S427R dimer binding ability of mutant heterozygotes was improved, thereby improving LS. The binding ability of mutant homozygous dimers was reduced, resulting in infertility. The results of this study will provide important references for animal breeding.</p></div>","PeriodicalId":54198,"journal":{"name":"Annals of Agricultural Science","volume":"68 2","pages":"Pages 148-158"},"PeriodicalIF":5.9,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0570178323000313/pdfft?md5=e533ed63422341bca1bc74576484e674&pid=1-s2.0-S0570178323000313-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138993932","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Utilizing tiger nut milk to make a novel, healthy, functional ice milk","authors":"Ola M.A.K. Shalabi","doi":"10.1016/j.aoas.2023.11.003","DOIUrl":"https://doi.org/10.1016/j.aoas.2023.11.003","url":null,"abstract":"<div><p>The study aimed to examine the effect of tiger nut milk on the quality characteristics of ice milk when fresh skim milk was partially replaced. At levels of substitution of 0, 25, 50, 75, and 100 %, the chemical and physical composition of the samples, the antioxidant and total phenolic content, the cytotoxic activity, the sensory properties, and the microstructure were evaluated. The results indicated that increasing the substitution levels of fresh skim milk for tiger nut milk significantly increased fat, fiber, antioxidant, total phenolic content, and hardness of the ice milk treatments (<em>P</em> &lt; 0.05), whereas their ash content, overrun and viscosity decreased. Furthermore, total solids, protein concentrations, or pH values between all treatments did not have significant effects (<em>P</em> &gt; 0.05). The IM1 treatment (ice milk without tiger nut milk) melted slower than the other treatments. Additionally, increasing the substitution levels of fresh skim milk for tiger nut milk decreased CaCo-2 cell viability (colorectal cancer cells). Furthermore, sensory properties were significantly affected (<em>P</em> &lt; 0.05) when fresh skim milk was replaced with tiger nut milk. The compact structure can be observed in the images of the treatments IM2 (75 % cow's skim milk +25 % tiger nut milk), IM3 (50 % cow's skim milk +50 % tiger nut milk), IM4 (25 % cow's skim milk +75 % tiger nut milk), and IM5 (100 % tiger nut milk), which were made with different ratios of tiger milk using scanning electron microscopy (SEM). On the other hand, the IM1 treatment image displays larger voids. Therefore, tiger nut milk offers a safe, plant-based alternative to cow's skim milk, provides a source of total phenolics and a natural antioxidant to make functional ice milk with good sensory acceptance, and can be used alongside colon anticancer therapy.</p></div>","PeriodicalId":54198,"journal":{"name":"Annals of Agricultural Science","volume":"68 2","pages":"Pages 118-125"},"PeriodicalIF":5.9,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0570178323000271/pdfft?md5=30ef59977bd4d31f92ef426f5e7ed1ca&pid=1-s2.0-S0570178323000271-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138502041","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multiple cutting increases forage productivity and enhances legume pasture stability in a rainfed agroecosystem","authors":"Tengfei Li ,&nbsp;Luxi Peng ,&nbsp;Hua Wang ,&nbsp;Yu Zhang ,&nbsp;Yingxin Wang ,&nbsp;Yunxiang Cheng ,&nbsp;Fujiang Hou","doi":"10.1016/j.aoas.2023.12.002","DOIUrl":"10.1016/j.aoas.2023.12.002","url":null,"abstract":"<div><p>Appropriate harvesting management is essential for sustainable forage production and improving nutritional quality. In this study, four forage crops, including <em>Medicago sativa</em> (Ms), <em>Vicia sativa</em> (Vs), <em>Avena sativa</em> (As), and <em>Sorghum Sudangrass</em> (Ss) were evaluated under two harvesting modes, i.e. single harvest and multiple cuttings. Results showed that multiple cuttings increased the forage yield of Ms., As, and Ss by 82 %–114 %, 7.5 %–51 %, and 21 %–150 % compared to the single harvest. The acid and neutral detergent fiber concentration decreased, while crude protein contents increased with multiple cuttings. Also, multiple cuttings increased the ether extract content of Ms. and Ss and reduced the ash content by 9.42 % to 33.04 % from 2015 to 2016. The variation range of legume forages' annual yield was 18.66 %–33.04 %, while that of gramineous forages was 9.42 %–25.98 %. The relative feeding value (RFV) of Ms. was higher than that of other forages under multiple cuttings. In general, multiple cuttings decreased the SOC content, but the effect was insignificant for the two leguminous forages. Correlations analysis showed a significant positive relationship of RFV and CP with precipitation while a negative relationship of ADF and NDF with temperature. Moreover, results of yield stability, contribution rate, and TOPSIS (Technique for Order Preference by Similarity to an Ideal Solution) analysis showed that the response of legume crops to multiple cutting was superior to that of gramineous crops. Overall, the cultivation of leguminous forage under multiple cuttings can provide a basis for the development of pastoral agriculture production and animal husbandry in arid regions of China.</p></div>","PeriodicalId":54198,"journal":{"name":"Annals of Agricultural Science","volume":"68 2","pages":"Pages 126-136"},"PeriodicalIF":5.9,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0570178323000295/pdfft?md5=05bb19acda501c3447792b7ce0c29d9f&pid=1-s2.0-S0570178323000295-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139014275","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bacillus velezensis WB invokes soil suppression of Fusarium oxysporum f. sp. niveum by inducing particular taxa","authors":"Shiqi Luo,&nbsp;Zhigang Wang,&nbsp;Weihui Xu","doi":"10.1016/j.aoas.2023.12.005","DOIUrl":"10.1016/j.aoas.2023.12.005","url":null,"abstract":"<div><p><em>Fusarium</em> wilt of watermelon caused by <em>Fusarium oxysporum</em> f. sp. <em>niveum</em> (Fon) results in a substantial economic losses in agriculture. Biological control agents (BCAs) help in controlling the infection of Fon. However, it remains unclear whether the application of BCAs can invoke soil suppression by inducing specific microbial groups concerned with disease suppression to synergistically resist fungal pathogens. The objective of this project was to determine the microbial mechanisms involved in disease suppression by BCAs. Here, we conducted pot experiments to explore <em>Fusarium</em> wilt in watermelon and soil microbial communities in response to different treatments. We found that the application of <em>Bacillus velezensis</em> WB enhanced disease suppression in nonsterilized soil. We further found that disease suppression in the WF treatment was connected to influence on the resident soil microbial communities, explicitly by causing an increase in <em>Hyphomicrobiaceae</em> and <em>Chitinophagaceae</em>. Pathogen suppression by the above two families was further studied in laboratory and pot experiments. The results showed that a synthetic community consisting of specific bacterial taxa effectively decreased the incidence of <em>Fusarium</em> wilt. <em>B. velezensis</em> WB introduction impacts the resident soil microbiome and invokes soil suppression by inducing resident microbes. Our results should help in designing synthetic microbial communities to improve soil function.</p></div>","PeriodicalId":54198,"journal":{"name":"Annals of Agricultural Science","volume":"68 2","pages":"Pages 159-170"},"PeriodicalIF":5.9,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0570178323000325/pdfft?md5=cff07c5daa32d02aa197758729ae335e&pid=1-s2.0-S0570178323000325-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139020728","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Vegetable waste extracts as enhancers of baculovirus infections","authors":"Blanca Martínez-Inda ,&nbsp;Oihane Simón ,&nbsp;Nerea Jiménez-Moreno ,&nbsp;Irene Esparza ,&nbsp;José Antonio Moler ,&nbsp;Primitivo Caballero ,&nbsp;Carmen Ancín-Azpilicueta","doi":"10.1016/j.aoas.2023.11.001","DOIUrl":"https://doi.org/10.1016/j.aoas.2023.11.001","url":null,"abstract":"<div><p>Vegetable waste extracts (VWE) contain a great variety of antioxidants such as polyphenols, which have shown to potentiate baculovirus infections, making them ingredients for pest control ingredients. In the present study, the mortality enhancement of different vegetable extracts obtained from food residues when combined with baculoviruses was evaluated. Extracts from spent coffee (E2), rosehip (E17), asparagus (E28), artichoke (E29), beet stalks (E32) and banana peel (E37) were selected as they increased mortality of Spodoptera littoralis nucleopolyhedrovirus (SpliNPV) in second instar <em>S. littoralis</em> larvae, when comparing with the virus inoculation alone. Extracts were assayed at 1 % w/v. In <em>S. littoralis</em>-SpliNPV system, the selected extracts reduced the median lethal concentration (LC₅₀) of SpliNPV against second instar larvae. The E37 extract presented the highest potentiation, as it reduced the LC50 13.61 times, while the rest of the extracts presented LC50 reductions from 3.71 to 7.72-fold. In <em>Spodoptera exigua-</em>SeMNPV (Spodoptera exigua multiple nucleopolyhedrovirus) system, none of the extracts decreased the LC₅₀ of SeMNPV. In contrast, in <em>Spodoptera frugiperda</em>-SfMNPV (Spodoptera frugiperda multiple nucleopolyhedrovirus) system, E2 showed the greatest potentiating effect. In the heterologous systems, none of the extracts tested increased the effective host range of SfMNPV, AcMNPV (Autographa californica multiple nucleopolyhedrovirus), and MbMNPV (Mamestra brassicae multiple nucleopolyhedrovirus) in second instar <em>S. littoralis</em> larvae. Thus, the viral enhancing effect of VWE was host-pathogen and instar dependent. However, the potentiation effect of the extracts could not be directly related with the antioxidants content of the extracts.</p></div>","PeriodicalId":54198,"journal":{"name":"Annals of Agricultural Science","volume":"68 2","pages":"Pages 96-107"},"PeriodicalIF":5.9,"publicationDate":"2023-11-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0570178323000258/pdfft?md5=740670593759e225ec6180f0bb3fe23c&pid=1-s2.0-S0570178323000258-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138435858","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An energy-saving glasshouse film reduces seasonal, and cultivar dependent Capsicum yield due to light limited photosynthesis","authors":"Sachin G. Chavan ,&nbsp;Xin He ,&nbsp;Chelsea Maier ,&nbsp;Yagiz Alagoz ,&nbsp;Sidra Anwar ,&nbsp;Zhong-Hua Chen ,&nbsp;Oula Ghannoum ,&nbsp;Christopher I. Cazzonelli ,&nbsp;David T. Tissue","doi":"10.1016/j.aoas.2023.04.001","DOIUrl":"https://doi.org/10.1016/j.aoas.2023.04.001","url":null,"abstract":"<div><p>Glasshouse films can be used to reduce energy costs by limiting non-productive heat-generating radiation, but the impact on yield of greenhouse horticultural crops remains unknown. The effects of energy-saving film ULR-80 (ultra-low-reflectivity film with 80 % light transmission referred to as Smart Glass; SG) designed to block long wavelength light that generates heat also reduced photosynthetically active radiation (PAR) consequently affecting crop morphology, photosynthesis, leaf pigments, and yield of two hydroponically grown capsicum (<em>Capsicum annuum</em> L.) cultivars (Red and Orange). The crops were grown in four high-tech glasshouse bays over two seasons of similar daily light integrals (DLI) during ascending [Autumn Experiment (AE)] and descending [Summer Experiment (SE)] photoperiods. The Red cultivar exhibited higher photosynthetic rates (light saturated - <em>A</em>_<em>sat</em>) and yield than the Orange cultivar in control glass but displayed stronger reductions in modelled photosynthetic rates at growth light and yield in SG without changes in photosynthetic capacity. Foliar pigment ratios of chlorophyll <em>a</em>/b and carotenoid:chlorophyll remained unaffected by the SG during both seasons indicating that chloroplast homeostasis was similar between SG and control. The seasonal differences in photosynthetic pigments and xanthophyll de-epoxidation state (DPS) revealed that cultivars were able to sense the SG-altered light environment during the AE, but not SE. The SE correlated with a lower daily light level and a substantial yield reduction of 29 % and 13 % in Red and Orange cultivars, respectively. Thus, SG-induced higher reductions in yield during the SE indicate that SG may be more beneficial for capsicum crops planted during AE.</p></div>","PeriodicalId":54198,"journal":{"name":"Annals of Agricultural Science","volume":"68 1","pages":"Pages 21-35"},"PeriodicalIF":5.9,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49810506","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Late nitrogen fertilization improves cotton yield through optimizing dry matter accumulation and partitioning","authors":"Yu Tian ,&nbsp;Fangyong Wang ,&nbsp;Xiaojuan Shi ,&nbsp;Feng Shi ,&nbsp;Nannan Li ,&nbsp;Junhong Li ,&nbsp;Karine Chenu ,&nbsp;Honghai Luo ,&nbsp;Guozheng Yang","doi":"10.1016/j.aoas.2023.06.001","DOIUrl":"10.1016/j.aoas.2023.06.001","url":null,"abstract":"<div><p>Cotton (<em>Gossypium hirsutum</em> L.) production efficiency is constrained by issues such as a low nitrogen (N) utilization rate (30–35 %) and high N application rate (300–350 kg ha⁻¹) in particular arid climates, such as Xinjiang, the largest irrigated cotton-producing region in China. These issues could be alleviated by allocating more N to late application to satisfy plant needs for growth and development. Over a three-year (2019–2021) field experiment, 240 kg ha⁻¹ N was applied in 3 periods (squaring, flowering to peak boll, and late peak boll). The amount of N applied in the second period was fixed at 60 %, and the remaining 40 % was split between the first and the third periods, with five ratios (treatments), i.e., 0:6:4 (N₀₆₄), 1:6:3 (N₁₆₃), 2:6:2 (N₂₆₂), 3:6:1 (N₃₆₁) and 4:6:0 (N₄₆₀), to investigate cotton response in terms of growth, biomass accumulation, and yield. Compared with the conventional treatment (N₂₆₂), the N₀₆₄ treatment narrowed the plant width by 11.7–12.0 %, increased canopy light transmittance by 6.1–56.9 %, extended the boll growth period by 8.8–9.4 %, improved defoliation by 9.3–11.7 % and increased both seed cotton yield and N partial factor productivity by 7.1–8.1 %, depending on the year. N₀₆₄ accumulated 4.3–39.5 % more biomass (K) than N₂₆₂, with the average and maximum growth rates of the reproductive organs (Vt and Vm, respectively) increasing by 8.6–89.0 % and 6.9–125.7 %, respectively, while the fast growth duration (∆t) shortened by 9.9–31.6 %. Again, N₀₆₄ partitioned 1.5–35.8 % more biomass to bolls (higher partitioning index, PI_boll). Seed cotton yield was significantly positively correlated with K, t₁, Vt, Vm and PI_boll and negatively correlated with ∆t in reproductive organs. Collectively, the data suggest that allocating more N to late application could be an efficient N fertilizer management strategy in arid areas under N-reduced cultivation and fertigation.</p></div>","PeriodicalId":54198,"journal":{"name":"Annals of Agricultural Science","volume":"68 1","pages":"Pages 75-86"},"PeriodicalIF":5.9,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48167037","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Differential proteomics highlights specific testicular proteins associated with chicken sperm motility and fertility potential","authors":"Yanyan Sun ,&nbsp;Yunlei Li ,&nbsp;Lei Shi ,&nbsp;Fuguang Xue ,&nbsp;Hong Xu ,&nbsp;Qingshi Meng ,&nbsp;Yifan Liu ,&nbsp;Yunhe Zong ,&nbsp;Hui Ma ,&nbsp;Jilan Chen","doi":"10.1016/j.aoas.2023.05.001","DOIUrl":"10.1016/j.aoas.2023.05.001","url":null,"abstract":"<div><p>Sperm motility, the percentage of sperm with forward progressions, is one of the semen quality traits that is highly related with male fertility potential in farm animals. The proportion of roosters with low sperm motility impedes the reproduction efficiency. This study aimed to determine crucial testicular proteins linked to sperm motility defects as causes of subfertility in roosters. iTRAQ was performed with the testis from five low sperm motility roosters and three high ones. A total of 2345 proteins were identified, of which, 86 were differentially expressed (DEPs), including 5 up-regulated and 88 down-regulated proteins in the low group. These DEPs were mainly enriched in cytoskeleton and cilium cell component, and biological processes related to sperm motility, and protein transport, and cellular protein localization. A cluster of 13 down-regulated proteins such as IFT88, TEKT1, ACTN2, DNAH5, RSPH9, and SPAG6, were associated with axoneme assembly. Further western blot and immunohistochemical analysis confirmed the down-regulated SPAG6 expression in low group, and indicated its expression in other cell types in testis beyond round spermatids, and that its pattern was in accordance with testis development and recession pace. Silencing transcription factor SOX5 down-regulated SPAG6 transcripts and impaired the cell proliferation and migration. In summary, this study highlights that down-regulated sperm flagellar structure associated proteins were the potential cause of low sperm motility. More specifically, the highly conserved SPAG6 protein across species is a positive regulator for testis development, spermatogenesis, and sperm motility regulation in chickens.</p></div>","PeriodicalId":54198,"journal":{"name":"Annals of Agricultural Science","volume":"68 1","pages":"Pages 36-47"},"PeriodicalIF":5.9,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43658271","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}