Annals of Agricultural Science最新文献

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Soil parameters, onion growth, physiology, biochemical and mineral nutrient composition in response to colored polythene film mulches 彩色聚乙烯薄膜覆盖对土壤参数、洋葱生长、生理生化及矿质养分组成的响应
IF 5.9 2区 农林科学
Annals of Agricultural Science Pub Date : 2019-06-01 DOI: 10.1016/j.aoas.2019.05.003
Md. Dulal Sarkar , Abul Hasnat Muhammad Solaiman , Mohammad Shah Jahan , Rojobi Nahar Rojoni , Khairul Kabir , Mirza Hasanuzzaman
{"title":"Soil parameters, onion growth, physiology, biochemical and mineral nutrient composition in response to colored polythene film mulches","authors":"Md. Dulal Sarkar ,&nbsp;Abul Hasnat Muhammad Solaiman ,&nbsp;Mohammad Shah Jahan ,&nbsp;Rojobi Nahar Rojoni ,&nbsp;Khairul Kabir ,&nbsp;Mirza Hasanuzzaman","doi":"10.1016/j.aoas.2019.05.003","DOIUrl":"10.1016/j.aoas.2019.05.003","url":null,"abstract":"<div><p>The present study evaluated the effect of different colored plastic mulching viz. olive polythene film (OPF), silver polythene film (SPF), white polythene film (WPF), black polythene film (BPF) and blue polythene film (BLPF) on soil properties, growth, physiology, biochemical and mineral nutrient composition in onion bulbs. Soil properties at 0–10 cm depth are variably affected by colored polythene film mulches. The highest values of soil moisture and pH were found when covered with a silver film, whereas black film provided the higher temperature among other types of film. The contents of nitrogen, phosphorus, potassium, and sulfur in the soils were slightly enhanced by mulching. Fresh biomass of shoot, bulb, and root was enhanced by silver colored mulching due to suitable moisture conditions, temperature and nutrients in the root zone. Plant height, number of leaves, bulb diameter and bulb length, were also remarkably increased after mulching where also the utmost values of dry biomass of shoot, bulb, and root were found. Under different colored polythene film mulches, a significant difference was found only for blue-yellow chromaticity (b*) while lightness (L*) and green-red chromaticity (a*) of onion skin did not affect significantly. With the mulching of olive colored polythene film, the skin of onion bulbs exhibited maximum b* values in compare to all mulches and control. Sugars, pyruvate and mineral nutrient concentration in onion bulbs also increased in all colored mulches whereas silver film provided the utmost value almost in all. Comparing all results, silver color polythene mulching is found to be the best choice for better onion growth, physiology, and quality.</p></div>","PeriodicalId":54198,"journal":{"name":"Annals of Agricultural Science","volume":"64 1","pages":"Pages 63-70"},"PeriodicalIF":5.9,"publicationDate":"2019-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.aoas.2019.05.003","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46536579","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 22
In vitro evaluation of pleiotropic properties of wild mushroom Laetiporus sulphureus 野生蘑菇硫生Laetiporus sulfureus多效性的体外评价
IF 5.9 2区 农林科学
Annals of Agricultural Science Pub Date : 2019-06-01 DOI: 10.1016/j.aoas.2019.05.001
Ahmed M. Younis , Mohamed Yosri , Jennifer K. Stewart
{"title":"In vitro evaluation of pleiotropic properties of wild mushroom Laetiporus sulphureus","authors":"Ahmed M. Younis ,&nbsp;Mohamed Yosri ,&nbsp;Jennifer K. Stewart","doi":"10.1016/j.aoas.2019.05.001","DOIUrl":"10.1016/j.aoas.2019.05.001","url":null,"abstract":"<div><p>In this study we extracted bioactive ingredients from fruiting bodies of <em>Laetiporus sulphureus</em> with different solvents and tested the antimicrobial and antitumor activities. The predominant lipid composition of the fruiting bodies was studied with GC–MS and revealed a high amount of oleic acid, octadecenoic acid and ergosterol derivatives. Extracts of <em>L</em>. <em>sulphureus</em> were shown to have antimicrobial activity against all fungal strains tested including <em>Candida albicans</em>, <em>Geotrichum candidum</em>, <em>Aspergillus fumigatus</em>, <em>Aspergillus niger</em>, <em>Curvularia clavata</em>, and <em>Fusarium oxysporum</em>, as well as strains of gram-positive (<em>Staphylococcus aureus</em> and <em>Streptococcus pyogenes</em>) and gram-negative bacteria (<em>Escherichia coli</em>, <em>Klebsiella pneumoniae</em>, <em>Pseudomonas aeruginosa</em> and <em>Shigella enterica</em>). Antimicrobial activity of ethanol and water extracts exceeded that of other extracts based on greater inhibitory activity at a lower minimum inhibitory concentration (MIC) range (MIC of ethanol = 15.6–62.5 μg/ml and of water = 15.6–125 μg/ml). The ethanol extract also exhibited superior anti-proliferative activity against three carcinoma cell lines and did not differ from a water extract against HeLa cells with half maximal inhibitory concentrations (IC<sub>50</sub>) of 3.1 ± 1.5 and 5.1 ± 1.2 μg/ml against HCT 116 and MCF-7 cells, respectively and IC<sub>50</sub> of 11.5 ± 2.1 and 12.1 ± 1.7 μg/ml against Hep G2 and HeLa cells, respectively. The extract inhibited growth of isolated normal mouse hepatocytes by &lt;12%. Scanning electron microscopy was applied to characterize the structures of the microbes treated with the ethanol semi-purified fraction. These findings suggest potential use <em>L. sulphureus</em> extract as a natural resource with anti-proliferative activity against tumor cells and microbial infections.</p></div>","PeriodicalId":54198,"journal":{"name":"Annals of Agricultural Science","volume":"64 1","pages":"Pages 79-87"},"PeriodicalIF":5.9,"publicationDate":"2019-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.aoas.2019.05.001","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48532042","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 19
Allelopathic potential of essential oils isolated from peels of three citrus species 三种柑橘果皮精油的化感作用
IF 5.9 2区 农林科学
Annals of Agricultural Science Pub Date : 2019-06-01 DOI: 10.1016/j.aoas.2019.04.003
Salma A. El Sawi , Mohamed E. Ibrahim , Kowthar Gad El-Rokiek , Samia Amin Saad El-Din
{"title":"Allelopathic potential of essential oils isolated from peels of three citrus species","authors":"Salma A. El Sawi ,&nbsp;Mohamed E. Ibrahim ,&nbsp;Kowthar Gad El-Rokiek ,&nbsp;Samia Amin Saad El-Din","doi":"10.1016/j.aoas.2019.04.003","DOIUrl":"10.1016/j.aoas.2019.04.003","url":null,"abstract":"<div><p>In this study the essential oils were extracted from the peels of <em>Citrus sinensis</em> (sweet orange), <em>Citrus aurantium</em> (bitter orange) and <em>Citrus reticulata</em> (mandarins) by hydrodistillation using a Clevenger- type apparatus for 4 h. The essential oils of all samples were subjected to Gas chromatography–mass spectrometry. The results indicated that the major compounds were <span>d</span>-limonene, terpinene and myrcene. Essential oils from these species were tested against seed germination and seedling growth of <em>Heliantus annus</em> (sunflower), <em>Portulaca oleracea</em> (purslane), <em>Lupinus albus</em> (field lupine) and <em>Malva parviflora</em> (Egyptian mallow) in laboratory experiments at different concentrations. The assay results showed that the essential oil extracted from <em>C</em>. <em>reticulata</em> peels was the most inhibitory followed by the essential oil extracted from <em>C</em>. <em>aurantium</em> and <em>C</em>. <em>sinensis</em> peels. Essential oils of <em>C</em>. <em>reticulata</em> and <em>C</em>. <em>aurantium</em> peels inhibited completely seed germination and seedling growth of <em>H</em>. <em>annus</em> at all concentrations. <em>Portulaca oleracea</em> seed germination and seedling growth were inhibited completely by essential oil of <em>C</em>. <em>aurantium</em> (2%). The essential oil of <em>C</em>. <em>sinensis</em> (3%) inhibited 100% of germination and growth of <em>Lupinus albus</em>. And. <em>M</em>. <em>parviflora</em> germination was 100% inhibited by <em>C</em>. <em>aurantium</em> (3%) and <em>C</em>. <em>reticulata</em> (1%) peel oils. The results obtained suggested that citrus peel essential oils present significant growth inhibitory property of the listed species and has potential to be used in allelopathic processes.</p></div>","PeriodicalId":54198,"journal":{"name":"Annals of Agricultural Science","volume":"64 1","pages":"Pages 89-94"},"PeriodicalIF":5.9,"publicationDate":"2019-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.aoas.2019.04.003","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43317358","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 16
Distillates composition obtained of fermented Arbutus unedo L. fruits from different seedlings and clonal plants 从不同幼苗和无性系植物中获得的发酵的Undutus unedo L.果实的蒸馏物组成
IF 5.9 2区 农林科学
Annals of Agricultural Science Pub Date : 2019-06-01 DOI: 10.1016/j.aoas.2019.05.009
Ilda Caldeira , Filomena Gomes , Helena Mira , Goreti Botelho
{"title":"Distillates composition obtained of fermented Arbutus unedo L. fruits from different seedlings and clonal plants","authors":"Ilda Caldeira ,&nbsp;Filomena Gomes ,&nbsp;Helena Mira ,&nbsp;Goreti Botelho","doi":"10.1016/j.aoas.2019.05.009","DOIUrl":"10.1016/j.aoas.2019.05.009","url":null,"abstract":"<div><p>The fruits of <em>Arbutus unedo</em> L., a shrub that grows spontaneously in countries around the Mediterranean basin, are traditionally harvested and used for the production of a distilled spirit. The main objective of this work is to compare the chemical profile of distillates obtained from fermented <em>A. unedo</em> fruits. Fifteen distinct distillates were obtained from ten clones and five seedlings, and they were evaluated, based on their physicochemical characteristics. An evident effect of plant propagation type (clone or seeds) on correspondent spirits analytical profile was not clearly achieved. However, the distillate composition variability seems connected to the plants variability. Furthermore, high significant effect of distillation time in all the variables measured was found, corroborating the importance of the splitting of the distillation fractions, as a good manufacturing practice. Regarding methanol, a harmful volatile compound, all the distillates obtained from the different clones or seedling fruits respected the legal limitations. From the principal component analysis, the first component seems to separate the distillates based on the pH and isobutanol, isoamyl alcohols and 2-phenylethanol which are the variables that presented the highest contributes to this component. The differences between the legal requirements and the amounts determined in these distillates could result from differences in the alcoholic fermentation microbiota.</p><p>The high analytical quality, that some of the distillates showed, can be used by producers as a criterion to be considered in the future choice of plants to be implemented in new <em>A. unedo</em> orchards.</p></div>","PeriodicalId":54198,"journal":{"name":"Annals of Agricultural Science","volume":"64 1","pages":"Pages 21-28"},"PeriodicalIF":5.9,"publicationDate":"2019-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.aoas.2019.05.009","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42572762","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 7
The identification of allergen proteins in two different varieties of strawberry by two different approaches: Proteomic and western blotting method 用蛋白质组学和western blotting两种不同的方法对两种不同品种草莓的过敏原蛋白进行鉴定
IF 5.9 2区 农林科学
Annals of Agricultural Science Pub Date : 2018-12-01 DOI: 10.1016/j.aoas.2018.11.003
Carmine Guarino, Rosaria Sciarrillo
{"title":"The identification of allergen proteins in two different varieties of strawberry by two different approaches: Proteomic and western blotting method","authors":"Carmine Guarino,&nbsp;Rosaria Sciarrillo","doi":"10.1016/j.aoas.2018.11.003","DOIUrl":"10.1016/j.aoas.2018.11.003","url":null,"abstract":"<div><p>Strawberry (<em>Fragaria ananassa</em> Duch.) is one of most important fruit crops cultivated widely in world. In the present study, we have investigated the proteome variation to different commercially available red strawberry varieties, to gain better insight into the variation in allergen content between the different varieties, as well as their proteome variation. In fact, a combination of proteomic tools have been used to build a complete allergen map of strawberry. The water-soluble fraction of a strawberry extract was precipitated using a phenol-based procedure and separated by 2-DE. Further, all known strawberry allergens were localized on a 2-DE map and they were matched with spots recognized by sera of patients with different allergic patterns. A number of constitutive or differentially accumulated proteins were found. The role of the identified proteins, in particular of the allergen proteins, is discussed in relation to the different variety of strawberry.</p></div>","PeriodicalId":54198,"journal":{"name":"Annals of Agricultural Science","volume":"63 2","pages":"Pages 181-189"},"PeriodicalIF":5.9,"publicationDate":"2018-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.aoas.2018.11.003","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46499688","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 7
Formulation of slow release NPK fertilizer (cellulose-graft-poly(acrylamide)/nano-hydroxyapatite/soluble fertilizer) composite and evaluating its N mineralization potential 缓释氮磷钾(纤维素-接枝-聚丙烯酰胺/纳米羟基磷灰石/可溶性肥料)复合肥料配方及氮矿化潜力评价
IF 5.9 2区 农林科学
Annals of Agricultural Science Pub Date : 2018-12-01 DOI: 10.1016/j.aoas.2018.11.001
Kiplangat Rop , George N. Karuku , Damaris Mbui , Immaculate Michira , Njagi Njomo
{"title":"Formulation of slow release NPK fertilizer (cellulose-graft-poly(acrylamide)/nano-hydroxyapatite/soluble fertilizer) composite and evaluating its N mineralization potential","authors":"Kiplangat Rop ,&nbsp;George N. Karuku ,&nbsp;Damaris Mbui ,&nbsp;Immaculate Michira ,&nbsp;Njagi Njomo","doi":"10.1016/j.aoas.2018.11.001","DOIUrl":"10.1016/j.aoas.2018.11.001","url":null,"abstract":"<div><p>Polymer nano-composite fertilizer formulation has the potential to enhance nutrient use efficiency. Slow release fertilizer (SRF) composite was formulated by incorporating nano-hydroxyapatite (nano-HA) and water soluble fertilizers (urea, (NH<sub>4</sub>)<sub>2</sub>HPO4 and K<sub>2</sub>SO<sub>4</sub>) into water hyacinth cellulose-graft-poly(acrylamide) polymer hydrogel. Fourier Transform Infra-red spectra revealed existence of chemical interaction between the monomer, cellulose, urea and nano-HA. The release of nutrients was assessed using laboratory incubation experiment. Significantly higher content of mineral nitrogen (MN) was observed in the first 4 weeks in conventional fertilizer (CF) compared to SRF treatments and the control. MN content in SRF treatments increased considerably between the 8th and 12th week, and declined in the 16th week. The values of potentially mineralizable N estimated using first order kinetics model related well to the observed cumulative MN at 16th week. No significant difference was observed between CF and SRF treatments for available P content in the 2nd week. Significantly higher P content was observed in CF compared to SRF treatment in the 4th week, whereas in the 8th week, some SRFs released significantly higher content than CF. Available P peaked in the 8th week in all the treatments and remained constant at 12th and 16th week. Availability of P in SRFs increased with increased content of soluble P and decreased content of nano-HA. Exchangeable K showed less variation during the incubation period, suggesting short release time. The data revealed reduced chances of leaching losses and toxic effect to the plant roots, as well as synchronized nutrient release and requirement by crops.</p></div>","PeriodicalId":54198,"journal":{"name":"Annals of Agricultural Science","volume":"63 2","pages":"Pages 163-172"},"PeriodicalIF":5.9,"publicationDate":"2018-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.aoas.2018.11.001","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42095662","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 50
Effect of tonophosphan, zinc oxide, and ascorbic acid on semen, sexual desire, and the fertility rate of Egyptian buffalo bulls 托磷磷、氧化锌和抗坏血酸对埃及水牛精液、性欲和生育率的影响
IF 5.9 2区 农林科学
Annals of Agricultural Science Pub Date : 2018-12-01 DOI: 10.1016/j.aoas.2018.12.001
Ahmed Abdel-wanees Gabr , Mohammed Fouad El Basuini
{"title":"Effect of tonophosphan, zinc oxide, and ascorbic acid on semen, sexual desire, and the fertility rate of Egyptian buffalo bulls","authors":"Ahmed Abdel-wanees Gabr ,&nbsp;Mohammed Fouad El Basuini","doi":"10.1016/j.aoas.2018.12.001","DOIUrl":"10.1016/j.aoas.2018.12.001","url":null,"abstract":"<div><p>A twenty weeks experimental trial was conducted to evaluate the effects of using tonophosphan (8 ml injection), zinc oxide (ZnO) at 0.8 g oral dose and ascorbic acid (AA) at 0.5% oral dose of live body weight on reproductive performance of Egyptian buffalo bulls (n = 20, 550–600 kg). The overall mean of all treated groups yielded marked improvement in all semen physical characteristics. Reaction time (RT), scrotal circumference, testicular volume, the concentration of semen fructose, testosterone, total proteins, and albumin in blood plasma were significantly (P ≤ 0.05) improved in all treatment groups compared with the control, but were greater in tonophosphan group. The highest concentrations of phosphorus and zinc in blood or semen were observed in tonophosphan and zinc treatments, respectively as compared to other groups. Overall mean of all semen physical characteristics and sperm outputs were not affected by advancing collection weeks. Meanwhile, the testosterone concentration and testicular volume increased (P ≤ 0.05), whereas the reaction time did not vary. The conception rate of buffalo cows was the highest (P ≤ 0.05) in tonophosphan (82.8%), followed by ascorbic acid and zinc (77.1 and 74.2%) as compared to the control group (60%). These findings suggested that tonophosphan, zinc oxide, and ascorbic acid supplementation improved the reproductive performance of Egyptian buffalo bulls; however, tonophosphan showed better performance.</p></div>","PeriodicalId":54198,"journal":{"name":"Annals of Agricultural Science","volume":"63 2","pages":"Pages 215-221"},"PeriodicalIF":5.9,"publicationDate":"2018-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.aoas.2018.12.001","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49143506","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 9
Effects of microwave heating on the antioxidant activities of tomato (Solanum lycopersicum) 微波加热对番茄抗氧化活性的影响
IF 5.9 2区 农林科学
Annals of Agricultural Science Pub Date : 2018-12-01 DOI: 10.1016/j.aoas.2018.09.001
Boumendjel Mahieddine , Benabdallah Amina , Samar Mohamed Faouzi , Bouras Sana , Dalli Wided
{"title":"Effects of microwave heating on the antioxidant activities of tomato (Solanum lycopersicum)","authors":"Boumendjel Mahieddine ,&nbsp;Benabdallah Amina ,&nbsp;Samar Mohamed Faouzi ,&nbsp;Bouras Sana ,&nbsp;Dalli Wided","doi":"10.1016/j.aoas.2018.09.001","DOIUrl":"10.1016/j.aoas.2018.09.001","url":null,"abstract":"<div><p>The aim of this study is to estimate phytochemical constituents and antioxidant activities of tomato slices treated by microwave heating. Two treatments time were applied, 30 and 300 s. Quantitative analyses of phenols, flavonoids, lycopene, followed by the evaluation of the antioxidant activity by DPPH (2,2-diphenyl-1-picrylhydrazyl) and ion chelating methods, were conducted on ethanolic extracts. Results showed the presence of these bioactive phytonutrients in fresh tomato and that their biodisponibility increases with the duration of the selected heat treatment. The phytochemical content showed important levels of polyphenols (1.92, 1.96, 6.95 mg gallic acid equivalent/g fresh matter) and flavonoids (2.33, 2.57, 10.68 mg RE/g fresh matter), in addition to lycopene (5.772, 31.8654, 40.71 mg/kg fresh matter) of non-treated, treated tomatoes at 30 s and treated tomatoes at 300 s, respectively. Results also revealed a high level of antioxidant activity by both methods with IC<sub>50</sub> of 1.69, 1.50, 1.33 µg/ml, for DPPH method and IC<sub>50</sub> of 401, 360.5, 299.91 µg/ml, for ion chelating ability. Thus, in our study, we demonstrated that microwave treatments enhance the nutritional quality of tomato slices by enhancing the biodisponibility of some of its content. Higher treatment time gives better results.</p></div>","PeriodicalId":54198,"journal":{"name":"Annals of Agricultural Science","volume":"63 2","pages":"Pages 135-139"},"PeriodicalIF":5.9,"publicationDate":"2018-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.aoas.2018.09.001","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44207255","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 20
A comparative study between one-factor-at-a-time and minimum runs resolution-IV methods for enhancing the production of polysaccharide by Stenotrophomonas daejeonensis and Pseudomonas geniculate 大田窄养单胞菌和膝状假单胞菌提高多糖产量的单因子-一次法和最小运行分辨率- iv法的比较研究
IF 5.9 2区 农林科学
Annals of Agricultural Science Pub Date : 2018-12-01 DOI: 10.1016/j.aoas.2018.11.002
Khadiga A. Abou-Taleb, Gehan F. Galal
{"title":"A comparative study between one-factor-at-a-time and minimum runs resolution-IV methods for enhancing the production of polysaccharide by Stenotrophomonas daejeonensis and Pseudomonas geniculate","authors":"Khadiga A. Abou-Taleb,&nbsp;Gehan F. Galal","doi":"10.1016/j.aoas.2018.11.002","DOIUrl":"10.1016/j.aoas.2018.11.002","url":null,"abstract":"<div><p>Two bacterial strains were isolated from compost sample from Egypt and identified based on morphological and molecular characteristics using 16S rRNA sequence as <em>Stenotrophomonas daejeonensis</em> B14 and <em>Pseudomonas geniculate</em> Y8. Optimization of fermentation factors was performed by using one-factor-at-a-time (OFAT) and minimum runs resolution-IV statistical (Min Run Res IV) approaches. OFAT technique achieved the maximum polysaccharide dry weight at 30.1 and 25.3 g/L from <em>S. daejeonensis</em> B14 and <em>P. geniculate</em> Y8, respectively on medium containing 25% (<em>w</em>/<em>v</em>) blackstrap molasses (BSM) and 1.5 g/L corn steep liquor (CSL). The optimum pH was 6.5 and 7.5 for <em>P. geniculate</em> Y8 and <em>S. daejeonensis</em> B14, respectively. This medium inoculated with 5% (<em>v</em>/<em>v</em>) of standard inoculum of each strain individually, then incubated at 30 °C using shake flasks at 200 rpm for 96 h of the incubation period, respectively. Whereas, Min Run Res IV approach was recording that 3, among 8 factors had a significant effect on biopolymer production by both strains being fermentation time (72 h), BSM concentration (250 g/L), and shaking speed (at 250 rpm). This method resulted in a 7.3 and 9.2% increase in polysaccharide yield from <em>S. daejeonensis</em> B14 and <em>P. geniculate</em> Y8, respectively as compared to OFAT. The biopolymer was partially characterized according to physiochemical properties as follows; white colour crystalline, soluble in hot water, low viscosity and its contents of ash, total carbohydrate, total reducing sugar and total protein are ranged from 5.5 to 7.8%, 44.7 to 42.4%, 14.1 to 13.5%, 1.5 to 2.4%, respectively.</p></div>","PeriodicalId":54198,"journal":{"name":"Annals of Agricultural Science","volume":"63 2","pages":"Pages 173-180"},"PeriodicalIF":5.9,"publicationDate":"2018-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.aoas.2018.11.002","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47205193","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 26
IFC Editorial Board IFC编委会
IF 5.9 2区 农林科学
Annals of Agricultural Science Pub Date : 2018-12-01 DOI: 10.1016/S0570-1783(18)30037-X
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