龙柏内生真菌的细胞毒性、抗氧化、抗菌研究及植物化学筛选

IF 3.5 2区 农林科学 Q1 AGRICULTURE, MULTIDISCIPLINARY
S.M. Neaz Mahmud , Md. Hossain Sohrab , Mst. Nadira Begum , Satyajit Roy Rony , Suriya Sharmin , Fatema Moni , Shammi Akhter , A.K.M. Mohiuddin , Farhana Afroz
{"title":"龙柏内生真菌的细胞毒性、抗氧化、抗菌研究及植物化学筛选","authors":"S.M. Neaz Mahmud ,&nbsp;Md. Hossain Sohrab ,&nbsp;Mst. Nadira Begum ,&nbsp;Satyajit Roy Rony ,&nbsp;Suriya Sharmin ,&nbsp;Fatema Moni ,&nbsp;Shammi Akhter ,&nbsp;A.K.M. Mohiuddin ,&nbsp;Farhana Afroz","doi":"10.1016/j.aoas.2020.12.003","DOIUrl":null,"url":null,"abstract":"<div><p>Endophytic fungi are a group of fungi that reside in the living plant without causing any disease symptoms. It shows symbiotic/mutualistic characteristics with host plants. Endophytic fungi may increase the host defense system against various pathogenic microorganisms and may encompass secondary metabolites of respective host plants. Isolation of endophytic fungi and its metabolites from <em>Justicia gendarussa</em> plant grown in Bangladesh is a recent and new approach. A total of seven endophytic fungi were isolated and purified from the leaf and bark part of the plant. All of the isolated endophytic fungi were identified up to genus level on the basis of macroscopic and microscopic characteristics and up to species level on the basis of molecular characteristics as <em>Colletotrichum gloeosporioides</em>, <em>Diaporthe pseudomangiferae</em>, <em>Pseudopestalotiopsis camelliae</em>-<em>sinensis</em>, <em>Colletotrichum gloeosporioides</em>, <em>Fusarium solani</em>, <em>Colletotrichum tropicale</em> and <em>Colletotrichum siamense</em>. Preliminary chemical screening of the fungal extracts by thin-layer chromatographic technique revealed the presence of various compounds such as flavonoids, anthraquinones, couramins, isocoumarins etc. Fungal strain <em>Pseudopestalotiopsis camelliae</em>-<em>sinensis</em> exhibited significant antiradical activity through DPPH scavenging method and moderate antimicrobial activity against a wide range of human pathogenic microbes. Using brine shrimp lethality bioassay, all the isolates showed strong cytotoxic activity, therefore suggested that all the extracts may have bioactive metabolites. Overall, these findings indicate that <em>Justicia gendarussa</em> and its associated endophytic fungi could be an important source for exploring potentially bioactive compounds or leads for the new drug candidates.</p></div>","PeriodicalId":54198,"journal":{"name":"Annals of Agricultural Science","volume":"65 2","pages":"Pages 225-232"},"PeriodicalIF":3.5000,"publicationDate":"2020-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.aoas.2020.12.003","citationCount":"15","resultStr":"{\"title\":\"Cytotoxicity, antioxidant, antimicrobial studies and phytochemical screening of endophytic fungi isolated from Justicia gendarussa\",\"authors\":\"S.M. Neaz Mahmud ,&nbsp;Md. Hossain Sohrab ,&nbsp;Mst. Nadira Begum ,&nbsp;Satyajit Roy Rony ,&nbsp;Suriya Sharmin ,&nbsp;Fatema Moni ,&nbsp;Shammi Akhter ,&nbsp;A.K.M. Mohiuddin ,&nbsp;Farhana Afroz\",\"doi\":\"10.1016/j.aoas.2020.12.003\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Endophytic fungi are a group of fungi that reside in the living plant without causing any disease symptoms. It shows symbiotic/mutualistic characteristics with host plants. Endophytic fungi may increase the host defense system against various pathogenic microorganisms and may encompass secondary metabolites of respective host plants. Isolation of endophytic fungi and its metabolites from <em>Justicia gendarussa</em> plant grown in Bangladesh is a recent and new approach. A total of seven endophytic fungi were isolated and purified from the leaf and bark part of the plant. All of the isolated endophytic fungi were identified up to genus level on the basis of macroscopic and microscopic characteristics and up to species level on the basis of molecular characteristics as <em>Colletotrichum gloeosporioides</em>, <em>Diaporthe pseudomangiferae</em>, <em>Pseudopestalotiopsis camelliae</em>-<em>sinensis</em>, <em>Colletotrichum gloeosporioides</em>, <em>Fusarium solani</em>, <em>Colletotrichum tropicale</em> and <em>Colletotrichum siamense</em>. Preliminary chemical screening of the fungal extracts by thin-layer chromatographic technique revealed the presence of various compounds such as flavonoids, anthraquinones, couramins, isocoumarins etc. Fungal strain <em>Pseudopestalotiopsis camelliae</em>-<em>sinensis</em> exhibited significant antiradical activity through DPPH scavenging method and moderate antimicrobial activity against a wide range of human pathogenic microbes. Using brine shrimp lethality bioassay, all the isolates showed strong cytotoxic activity, therefore suggested that all the extracts may have bioactive metabolites. Overall, these findings indicate that <em>Justicia gendarussa</em> and its associated endophytic fungi could be an important source for exploring potentially bioactive compounds or leads for the new drug candidates.</p></div>\",\"PeriodicalId\":54198,\"journal\":{\"name\":\"Annals of Agricultural Science\",\"volume\":\"65 2\",\"pages\":\"Pages 225-232\"},\"PeriodicalIF\":3.5000,\"publicationDate\":\"2020-12-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/j.aoas.2020.12.003\",\"citationCount\":\"15\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Annals of Agricultural Science\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0570178320300543\",\"RegionNum\":2,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"AGRICULTURE, MULTIDISCIPLINARY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Annals of Agricultural Science","FirstCategoryId":"97","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0570178320300543","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"AGRICULTURE, MULTIDISCIPLINARY","Score":null,"Total":0}
引用次数: 15

摘要

内生真菌是一组寄生在活的植物中而不引起任何疾病症状的真菌。它与寄主植物具有共生/互惠的特点。内生真菌可以增强宿主对各种致病微生物的防御系统,并可能包含各自宿主植物的次生代谢物。从生长于孟加拉国的金针叶植物中分离内生真菌及其代谢物是一种新方法。从该植物的叶和皮中分离纯化了7种内生真菌。所有内生真菌经宏观和微观特征鉴定为属级,分子特征鉴定为种级,分别为炭疽菌(Colletotrichum gloeosporioides)、假金枝菌(Diaporthe pseudomangiferae)、茶树伪拟拟多毛孢(Pseudopestalotiopsis camellie -sinensis)、炭疽菌(Colletotrichum gloeosporioides)、茄枯菌(Fusarium solani)、热带炭疽菌(Colletotrichum tropicale)和siamense。通过薄层色谱技术对真菌提取物进行初步化学筛选,发现其含有黄酮类化合物、蒽醌类化合物、couramins、异香豆素类化合物等。真菌菌株camellie -sinensis通过DPPH清除法显示出显著的抗自由基活性,对多种人类病原微生物具有中等抑菌活性。结果表明,所有分离物均具有较强的细胞毒活性,表明其提取物可能具有生物活性代谢物。综上所述,这些发现表明,金针菇及其相关内生真菌可能是探索潜在生物活性化合物或新候选药物线索的重要来源。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Cytotoxicity, antioxidant, antimicrobial studies and phytochemical screening of endophytic fungi isolated from Justicia gendarussa

Endophytic fungi are a group of fungi that reside in the living plant without causing any disease symptoms. It shows symbiotic/mutualistic characteristics with host plants. Endophytic fungi may increase the host defense system against various pathogenic microorganisms and may encompass secondary metabolites of respective host plants. Isolation of endophytic fungi and its metabolites from Justicia gendarussa plant grown in Bangladesh is a recent and new approach. A total of seven endophytic fungi were isolated and purified from the leaf and bark part of the plant. All of the isolated endophytic fungi were identified up to genus level on the basis of macroscopic and microscopic characteristics and up to species level on the basis of molecular characteristics as Colletotrichum gloeosporioides, Diaporthe pseudomangiferae, Pseudopestalotiopsis camelliae-sinensis, Colletotrichum gloeosporioides, Fusarium solani, Colletotrichum tropicale and Colletotrichum siamense. Preliminary chemical screening of the fungal extracts by thin-layer chromatographic technique revealed the presence of various compounds such as flavonoids, anthraquinones, couramins, isocoumarins etc. Fungal strain Pseudopestalotiopsis camelliae-sinensis exhibited significant antiradical activity through DPPH scavenging method and moderate antimicrobial activity against a wide range of human pathogenic microbes. Using brine shrimp lethality bioassay, all the isolates showed strong cytotoxic activity, therefore suggested that all the extracts may have bioactive metabolites. Overall, these findings indicate that Justicia gendarussa and its associated endophytic fungi could be an important source for exploring potentially bioactive compounds or leads for the new drug candidates.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
Annals of Agricultural Science
Annals of Agricultural Science AGRICULTURE, MULTIDISCIPLINARY-
CiteScore
12.60
自引率
0.00%
发文量
18
审稿时长
33 days
期刊介绍: Annals of Agricultural Sciences (AOAS) is the official journal of Faculty of Agriculture, Ain Shams University. AOAS is an open access peer-reviewed journal publishing original research articles and review articles on experimental and modelling research at laboratory, field, farm, landscape, and industrial levels. AOAS aims to maximize the quality of the agricultural sector across the globe with emphasis on the Arabian countries by focusing on publishing the high-quality applicable researches, in addition to the new methods and frontiers leading to maximizing the quality and quantity of both plant and animal yield and final products.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信