Differential proteomics highlights specific testicular proteins associated with chicken sperm motility and fertility potential

IF 3.5 2区 农林科学 Q1 AGRICULTURE, MULTIDISCIPLINARY
Yanyan Sun , Yunlei Li , Lei Shi , Fuguang Xue , Hong Xu , Qingshi Meng , Yifan Liu , Yunhe Zong , Hui Ma , Jilan Chen
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引用次数: 1

Abstract

Sperm motility, the percentage of sperm with forward progressions, is one of the semen quality traits that is highly related with male fertility potential in farm animals. The proportion of roosters with low sperm motility impedes the reproduction efficiency. This study aimed to determine crucial testicular proteins linked to sperm motility defects as causes of subfertility in roosters. iTRAQ was performed with the testis from five low sperm motility roosters and three high ones. A total of 2345 proteins were identified, of which, 86 were differentially expressed (DEPs), including 5 up-regulated and 88 down-regulated proteins in the low group. These DEPs were mainly enriched in cytoskeleton and cilium cell component, and biological processes related to sperm motility, and protein transport, and cellular protein localization. A cluster of 13 down-regulated proteins such as IFT88, TEKT1, ACTN2, DNAH5, RSPH9, and SPAG6, were associated with axoneme assembly. Further western blot and immunohistochemical analysis confirmed the down-regulated SPAG6 expression in low group, and indicated its expression in other cell types in testis beyond round spermatids, and that its pattern was in accordance with testis development and recession pace. Silencing transcription factor SOX5 down-regulated SPAG6 transcripts and impaired the cell proliferation and migration. In summary, this study highlights that down-regulated sperm flagellar structure associated proteins were the potential cause of low sperm motility. More specifically, the highly conserved SPAG6 protein across species is a positive regulator for testis development, spermatogenesis, and sperm motility regulation in chickens.

差异蛋白质组学强调与鸡精子活力和生育潜力相关的特定睾丸蛋白
精子活力,即精子向前发展的百分比,是与雄性动物生育能力高度相关的精液质量特征之一。精子活力低的公鸡比例影响了繁殖效率。这项研究的目的是确定与精子运动缺陷有关的关键睾丸蛋白,这是公鸡生育能力低下的原因。iTRAQ是用5只精子活力低的公鸡和3只精子活力高的公鸡的睾丸进行的。共鉴定出2345个蛋白,其中差异表达(dep)蛋白86个,其中低表达组上调5个,下调88个。这些DEPs主要富集于细胞骨架和纤毛细胞组分,以及与精子运动、蛋白质转运和细胞蛋白质定位有关的生物过程。一组13个下调蛋白,如IFT88、TEKT1、ACTN2、DNAH5、RSPH9和SPAG6,与轴突蛋白组装有关。进一步的western blot和免疫组化分析证实了低水平组中SPAG6的表达下调,并表明其在睾丸除圆形精子外的其他细胞类型中均有表达,其表达模式与睾丸发育和衰退速度一致。沉默转录因子SOX5可下调SPAG6转录物,损害细胞增殖和迁移。总之,本研究强调了精子鞭毛结构相关蛋白的下调是精子活力低下的潜在原因。更具体地说,跨物种高度保守的SPAG6蛋白是鸡睾丸发育、精子发生和精子运动调节的积极调节因子。
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来源期刊
Annals of Agricultural Science
Annals of Agricultural Science AGRICULTURE, MULTIDISCIPLINARY-
CiteScore
12.60
自引率
0.00%
发文量
18
审稿时长
33 days
期刊介绍: Annals of Agricultural Sciences (AOAS) is the official journal of Faculty of Agriculture, Ain Shams University. AOAS is an open access peer-reviewed journal publishing original research articles and review articles on experimental and modelling research at laboratory, field, farm, landscape, and industrial levels. AOAS aims to maximize the quality of the agricultural sector across the globe with emphasis on the Arabian countries by focusing on publishing the high-quality applicable researches, in addition to the new methods and frontiers leading to maximizing the quality and quantity of both plant and animal yield and final products.
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