Revista Peruana de Medicina de Experimental y Salud Publica最新文献

{"title":"Actividad antiinflamatoria in vitro de Plantago major L. y Piper aduncum L. sobre la fosfolipasa A2 del veneno de la serpiente Lachesis muta muta","authors":"Mirtha Yarleque-Chocas, Flor Dorregaray-Llerena, Armando Yarleque-Chocas, Celso Gonzales-Chavesta","doi":"10.17843/rpmesp.2023.403.12191","DOIUrl":"https://doi.org/10.17843/rpmesp.2023.403.12191","url":null,"abstract":"Objetivo. Evaluar la actividad inhibitoria in vitro de los extractos de Plantago major «llantén» y Piper aduncum«matico» sobre Fosfolipasa A2 (PLA2) del veneno de la serpiente Lachesis muta muta. Materiales y métodos. Estainvestigación fue de tipo explicativa con diseño experimental. Se recolectaron hojas de P. major y P. aduncum en la provincia de Huarochirí en Lima, Perú. Se prepararon extractos alcohólicos diluidos en agua destilada y se realizaron los ensayos fitoquímicos, la cuantificación de fenoles y flavonoides, la cromatografía de capa fina (CCF) en celulosa y la actividad enzimática con PLA2. Se analizó la capacidad de inhibir la PLA2 con los extractos en estudio y sus fracciones. Para el análisis estadístico se utilizó la prueba de Kruskal Wallis y comparaciones múltiples de Bonferroni. Resultados. Tanto en P. major como en P. aduncum se identificó cualitativamente la presencia de fenoles, flavonoides y taninos; además, P. aduncum presentó saponinas. La inhibición de la actividad de la PLA2 del veneno por el extracto total de P. major fue del 45,3%, y sus fracciones mostraron valores de inhibición: LLF-1 con 31,1%, LLF-2 con 66,3% y LLF-3 con 65,5%. En P. aduncum, los valores de inhibición para el extracto total fueron de 86,9%, y sus fracciones presentaron inhibiciones: MF-1 con 34,3%, MF-2 con 67,1% y MF-3 con 54,9%. El análisis estadístico demostró diferencias significativas en la inhibición de la PLA2 (p=0,009) por los extractos. Conclusión. Los ensayos realizados demostraron una asociación entre el efecto antiinflamatorio de los extractos y la inhibición de la PLA2.","PeriodicalId":53651,"journal":{"name":"Revista Peruana de Medicina de Experimental y Salud Publica","volume":"127 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135721202","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Emergencia sanitaria por dengue en el Perú: necesidad de una asignación presupuestal acorde a la situación epidemiológica","authors":"Nilthon Pisfil-Benites, Stalin Vilcarromero, Diego Azañedo","doi":"10.17843/rpmesp.2023.403.13117","DOIUrl":"https://doi.org/10.17843/rpmesp.2023.403.13117","url":null,"abstract":"","PeriodicalId":53651,"journal":{"name":"Revista Peruana de Medicina de Experimental y Salud Publica","volume":"36 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135721626","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development and validation of a vero cell-based suspension method for the detection of Zika virus","authors":"Dina Popuche, Alfredo Huaman, Steev Loyola, María Silva, Sarah A. Jenkins, Carolina Guevara","doi":"10.17843/rpmesp.2023.403.12606","DOIUrl":"https://doi.org/10.17843/rpmesp.2023.403.12606","url":null,"abstract":"Objective. To develop and validate a cell suspension method using Vero 76 cells for culturing Zika virus (ZIKV) based on infection of detached freshly seeded cells. Material and methods. Three different multiplicities of ZIKV infection were used to develop and compare this novel method to the standard confluent cell monolayer method. In addition, we preliminary validated the cell suspension method using well-characterized ZIKV positive and negative clinical samples. The standard confluent cell monolayermethod was used as the reference method, and viral isolation was confirmed by a ZIKV-specific RT-PCR. We estimated the sensitivity, and its 95% confidence intervals, for the cell suspension method. Also, a technical comparison of the cell suspension method against the cell monolayer method was performed. Results. Our findings suggest that both the viral load and replication of ZIKV were comparable between both monolayer- and suspension-infection methods. Although both methods were suitable for culturing and isolating ZIKV, the cell suspension method was easier, cheaper, and quicker as well as a sensitive isolation technique. The cell suspension method was significantly more sensitive in detecting Zika in inconclusive cases by RT-PCR, with a fourfold increase compared to the confluent cell monolayer method. Conclusion. The cell suspension method has the potential to be an effective method for cultivating and isolating ZIKV and its application is potentially useful in both research and clinical settings.","PeriodicalId":53651,"journal":{"name":"Revista Peruana de Medicina de Experimental y Salud Publica","volume":"65 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135721627","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluación del neurodesarrollo en niños con exposición intrauterina al virus del Zika: estudio observacional transversal","authors":"Victor H. Estupiñan-Perez, Angela M. Jiménez-Urrego, Freiser E. Cruz-Mosquera, Alejandro Botero-Carvajal","doi":"10.17843/rpmesp.2023.403.12880","DOIUrl":"https://doi.org/10.17843/rpmesp.2023.403.12880","url":null,"abstract":"El virus del Zika produce desenlaces adversos para el desarrollo del sistema nervioso. Este estudio describe el neurodesarrollo cognitivo, adaptativo, comunicativo, social y motor de niños expuestos intrauterinamente al virus del Zika y hace una evaluación del neurodesarrollo con la escala de Battelle a los tres años después del nacimiento. Participaron 30 niños con una media de edad al momento de la evaluación de 37,5 (RIC: 35,7–39,2) meses. Se halló una edad equivalente en meses en las áreas: motora 25,8 (DE:7,8), adaptativa 26,7 (DE: 5,8), comunicativa 30,2 (DE: 6,9), personal social 33,5 (DE: 8,3) y cognitiva 35,6 (DE: 5,9). Los niños presentaron retraso en el desarrollo para la edad cronológica, 25 niños presentaban retraso en una de las cinco áreas evaluadas. Una alta proporción de niños expuestos al virus del Zika durante la gestación presentaron retraso en el desarrollo, principalmente en el dominio adaptativo y motor.","PeriodicalId":53651,"journal":{"name":"Revista Peruana de Medicina de Experimental y Salud Publica","volume":"127 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135721201","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cambios en el control glucémico antes y después de la cuarentena por la COVID-19","authors":"Javier R. Murillo-Valle, Juan L. Meza-Ponte","doi":"10.17843/rpmesp.2023.403.12830","DOIUrl":"https://doi.org/10.17843/rpmesp.2023.403.12830","url":null,"abstract":"","PeriodicalId":53651,"journal":{"name":"Revista Peruana de Medicina de Experimental y Salud Publica","volume":"33 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135866647","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Experiencias de usuarios y familiares sobre la atención recibida en centros de salud mental comunitaria de Lima y Callao durante la pandemia de la COVID-19","authors":"Noelia Cusihuaman-Lope, Ana L. Vilela-Estrada, Victoria Cavero, David Villarreal-Zegarra, Francisco Diez-Canseco","doi":"10.17843/rpmesp.2023.403.12717","DOIUrl":"https://doi.org/10.17843/rpmesp.2023.403.12717","url":null,"abstract":"Objetivos. Comprender las experiencias de usuarios nuevos y continuadores de los Centros de Salud Mental Comunitaria (CSMC) de Lima y Callao, y de sus familiares, en relación a la atención en salud mental que recibieron durante la pandemia de la COVID-19. Materiales y métodos. Estudio cualitativo realizado entre septiembre del 2021 y febrero del 2022, en el que se entrevistó a 24 usuarios y familiares que interactuaron con los servicios brindados por tres CSMC de Lima y uno del Callao, durante la pandemia de la COVID-19. Se realizó un análisis temático de las entrevistas transcritas. Resultados. Los informantes percibieron que la pandemia exacerbó los síntomas de las personas con problemas de salud mental. Durante la pandemia, las atenciones de salud mental se apoyaron en el uso de tecnología, principalmente de llamadas telefónicas, las que sirvieron para monitorear el estado emocional y el tratamiento farmacológico de los usuarios, así como para programar y recordar citas. Los usuarios destacan que las llamadas telefónicas frecuentes les hicieron sentirse acompañados y resaltan el compromiso de los trabajadores de los CSMC. Como dificultades, reportan el incremento en la demanda de atención, problemas para acceder a videollamadas, y menor calidad en las atenciones virtuales. Conclusiones. La COVID-19 impactó emocionalmente a las personas con problemas de salud mental, a su vez, los servicios de los CSMC vieron afectada la modalidad (presencial o virtual), recursos, frecuencia, tiempo y calidad de la atención, encontrando limitaciones y beneficios en el uso de la tecnología.","PeriodicalId":53651,"journal":{"name":"Revista Peruana de Medicina de Experimental y Salud Publica","volume":"13 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135866392","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Primera descripción de infección por el subcomplejo Acinetobacter pittii / lactucae en el Perú","authors":"Carla Andrea Alonso, Jorge Choque-Matos, Fernando Guibert, Beatriz Rojo-Bezares, María López, Rocio Egoávil-Espejo, Patricia Gonzales, Carmen Valera-Krumdieck, Maria J. Pons, Yolanda Saénz, Joaquim Ruiz","doi":"10.17843/rpmesp.2023.403.12721","DOIUrl":"https://doi.org/10.17843/rpmesp.2023.403.12721","url":null,"abstract":"","PeriodicalId":53651,"journal":{"name":"Revista Peruana de Medicina de Experimental y Salud Publica","volume":"43 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135866393","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Réplica a la Carta al Editor \"Consideraciones sobre la rinitis alérgica asociada al grado de compromiso pulmonar por COVID-19 en pacientes de un hospital general peruano\"","authors":"Bianca García-Gallo, Giancarlo Gonzales-Caldas, Diego Urrunaga-Pastor, Percy Herrera-Añazco","doi":"10.17843/rpmesp.2023.402.12959","DOIUrl":"https://doi.org/10.17843/rpmesp.2023.402.12959","url":null,"abstract":"","PeriodicalId":53651,"journal":{"name":"Revista Peruana de Medicina de Experimental y Salud Publica","volume":"27 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136185510","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development and validation of a COVID-19 risk perception scale in Peru.","authors":"Jhon Alex Zeladita-Huaman, Eduardo Franco-Chalco, Roberto Zegarra-Chapoñan, Ruth Iguiñiz-Romero, Isabel Amemiya-Hoshi","doi":"10.17843/rpmesp.2023.402.12289","DOIUrl":"10.17843/rpmesp.2023.402.12289","url":null,"abstract":"<p><strong>Objectives.: </strong>Motivation for the study. Risk perception of COVID-19 is a construct that varies according to the characteristics of the population in each geographic area; however, there is no validated scale to measure this construct in the Peruvian population. Main findings. A COVID-19 risk perception scale composed of two dimensions (cognitive and emotional) was designed and validated using qualitative and quantitative techniques. Implications. Having a valid and reliable instrument will help identify the variation of risk perception of COVID-19 according to contextual and psychological factors in the Peruvian population. . To develop and validate a risk perception scale for COVID-19 (PR-COVID-19-PE) in the Peruvian population.</p><p><strong>Materials and methods.: </strong>Psychometric cross-sectional study conducted in 2022. In phase 1, in order to design the scale, we carried out a theoretical review and a documentary review of scales, we also used focus groups as well as an expert panel. Phase 2 included expert judgment and a pilot test. A virtual survey was conducted among 678 Peruvian adults during phase 3. A confirmatory factor analysis was carried out as well. We used a correlational analysis (Pearson's r) with a valid risk perception scale and the COVID-19 fear scale to determine criterion validity.</p><p><strong>Results.: </strong>The PR-COVID-19-PE has two dimensions (cognitive and emotional) and showed good fit during construct validity (x2/gl=2.34, Comparative Fit Index=0.96, Tucker-Lewis Index=0.96, Root Mean Square Error of Approximation= 0.05 and Standardized Root Mean-Square=0.07) and optimal internal consistency (ώ=0.88). Likewise, the PR-COVID-19-PE showed correlation with another COVID-19 risk perception scale (r=0.70, p< 0.001) and a fear of COVID-19 scale (r=0.41, p<0.001). In addition, it presents metric and scalar invariance by both sex and educational level.</p><p><strong>Conclusions.: </strong>The PR-COVID-19-PE scale showed adequate reliability and content, construct and criterion validity. It is an instrument that can measure COVID-19 risk perception in similar populations. However, further studies are required for different populations.</p>","PeriodicalId":53651,"journal":{"name":"Revista Peruana de Medicina de Experimental y Salud Publica","volume":" ","pages":"170-178"},"PeriodicalIF":0.0,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10953650/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47985095","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Samples in randomized clinical trials with interim analysis.","authors":"Michelle Saaibi Meléndez, Felipe Botero-Rodríguez, Carlos Javier Rincón Rodríguez","doi":"10.17843/rpmesp.2023.402.12217","DOIUrl":"10.17843/rpmesp.2023.402.12217","url":null,"abstract":"<p><strong>Objectives.: </strong>This article introduces randomized clinical trials and basic concepts of statistical inference. We present methods for calculating the sample size by outcome type and the hypothesis to be tested, together with the code in the R programming language. We describe four methods for adjusting the original sample size for interim analyses. We sought to introduce these topics in a simple and concrete way, considering the mathematical expressions that support the results and their implementation in available statistical programs; therefore, bringing health students closer to statistics and the use of statistical programs, which are aspects that are rarely considered during their training.</p>","PeriodicalId":53651,"journal":{"name":"Revista Peruana de Medicina de Experimental y Salud Publica","volume":" ","pages":"220-228"},"PeriodicalIF":0.0,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10953660/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49005749","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}