Journal of Tropical Biodiversity and Biotechnology最新文献_第2页

Identifying Single Nucleotide Polymorphisms (SNPs) in OsFER1 and OsFER2 Genes Linked to Iron accumulation in Pigmented Indonesian Rice (Oryza sativa L.) 鉴定与印度尼西亚色素稻（Oryza sativa L.）铁积累有关的 OsFER1 和 OsFER2 基因中的单核苷酸多态性 (SNP)

Journal of Tropical Biodiversity and Biotechnology Pub Date : 2024-02-19 DOI: 10.22146/jtbb.78019

Apriliana Pratiwi, Rizka Fahma Bassalamah, Sabila Elvani, Alfino Sebastian, Y. A. Purwestri

{"title":"Identifying Single Nucleotide Polymorphisms (SNPs) in OsFER1 and OsFER2 Genes Linked to Iron accumulation in Pigmented Indonesian Rice (Oryza sativa L.)","authors":"Apriliana Pratiwi, Rizka Fahma Bassalamah, Sabila Elvani, Alfino Sebastian, Y. A. Purwestri","doi":"10.22146/jtbb.78019","DOIUrl":"https://doi.org/10.22146/jtbb.78019","url":null,"abstract":"Iron (Fe) is an essential micronutrient for the well-being of plants, animals, and bacteria. In plants, iron plays a pivotal role in a myriad of metabolic processes, encompassing redox reaction, photosynthesis, respiration, chlorophyll synthesis, and nitrogen fixation. For humans, iron is indespensable for several metabolic functions, particularly in the synthesis of haemoglobin. Iron deficiency can lead to health issues on a global scale, therefore identifying key crops, such as rice for providing sufficient iron in diet intake is very important. In rice, the maintenance of iron homeostasis is orchestrated by various genes, with OsFER1 and OsFER2 acting as iron accumulator genes in leaves, stems, flowers, and grains. The primary objective of this study was to ascertain the single nucleotide polymorphisms (SNP) in the OsFER1 and OsFER2 and to assess the iron content in Indonesian local rice cultivars. To achieve this, we examined partial sequences of OsFER1 and OsFER2 to identify SNPs in the Indonesian rice cultivars used (Cempo Ireng, Pari Ireng, Hitam Kalsel, Merah Pari Eja, and Ciherang). Concurrently, the iron content in the seeds was quantified using Atomic Absorption Spectrophotometry (AAS). The analysis revealed that the OsFER1 gene sequence, specifically exon 5, exhibited a SNP in the form of a transition. In contrast, the OsFER2 gene sequences, specifically in intron 2 displayed SNPs in the form of insertions. Notably, the iron content in the seeds was highest in Cempo Ireng (black rice), while it was lowest in Merah Pari Eja (red rice) and Ciherang (non-pigmented rice). Importantly, the identified SNPs in these partial gene sequences did not exert any discernible influence on iron levels or the formation of ferritin protein. ","PeriodicalId":52402,"journal":{"name":"Journal of Tropical Biodiversity and Biotechnology","volume":"56 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140452078","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Plant Growth Promoting Endophytic Microorganisms from Orchids for A Sustainable Agriculture 兰花中的植物生长促进内生微生物为可持续农业服务

Journal of Tropical Biodiversity and Biotechnology Pub Date : 2024-02-16 DOI: 10.22146/jtbb.74403

L. P. W. Goh, Benardette Lyovine Jaisi, R. Jawan, J. Gansau

引用次数: 0

Scanning Electron Microscopy Analysis of Tea’s Embryo Axis Explant Cultured on Murashige and Skoog Medium Containing 2,4-Dichlorophenoxyacetic acid 在含有 2,4-二氯苯氧乙酸的 Murashige 和 Skoog 培养基上培养的茶树胚轴外植体的扫描电子显微镜分析

Journal of Tropical Biodiversity and Biotechnology Pub Date : 2024-02-12 DOI: 10.22146/jtbb.76451

Ratna Dewi Eskundari, Taryono Taryono, D. Indradewa, Y. A. Purwestri

引用次数: 0

Lessons from the Mass Production of Wolbachia-infected Aedes aegypti for Egg Release in the Sleman and Bantul Districts of Yogyakarta 日惹 Sleman 和 Bantul 地区大规模生产受沃尔巴乔病毒感染的埃及伊蚊用于产卵的经验教训

Journal of Tropical Biodiversity and Biotechnology Pub Date : 2024-02-09 DOI: 10.22146/jtbb.84753

I. Fitriana, Indah Nurhayati, B. Arianto, Defriana Lutfi Chusnaifah, Indira Diah Utami, Nabhela Ayu Purwaningrum, Utari Saraswati, Endah Supriyati, Adi Utarini, R. Ahmad, C. Indriani, E. Arguni, W. Tantowijoyo

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Lead (Pb)-Resistant Bacteria Improve Brassica chinensis Biomass and Reduce Pb Concentration in Pb-Contaminated Soil 抗铅（Pb）细菌可提高芥蓝的生物量并降低铅污染土壤中的铅浓度

Journal of Tropical Biodiversity and Biotechnology Pub Date : 2024-02-05 DOI: 10.22146/jtbb.86174

Beauty Laras Setia Pertiwi, Reni Ustiatik, Yulia Nuraini

{"title":"Lead (Pb)-Resistant Bacteria Improve Brassica chinensis Biomass and Reduce Pb Concentration in Pb-Contaminated Soil","authors":"Beauty Laras Setia Pertiwi, Reni Ustiatik, Yulia Nuraini","doi":"10.22146/jtbb.86174","DOIUrl":"https://doi.org/10.22146/jtbb.86174","url":null,"abstract":"Applications of inorganic fertilisers and pesticides frequently increase lead (Pb) content in the soil and food crops. This study aims to isolate Pb-resistant bacteria and test the isolated bacteria in reducing Pb concentration and increasing biomass production of Brassica chinensis on Pb-contaminated soil. Soil and plant samples were collected from agricultural land in Batu City, East Java, Indonesia. The isolated bacteria were tested for Pb resistance and then characterised according to 16S rRNA Sequence. A pot trial with a completely randomised block design consisting of 9 treatments and 3 replications was set to determine the effect of Pb-resistant bacteria inoculation on Pb residue, plant growth, and soil nutrients. The result showed that the isolated Pb-resistant bacteria were Bacillus wiedmannii and Bacillus altitudinis. The bacteria were resistant to Pb up to 10,000 mg/L PbNO3. Inoculation of the bacteria increased B. chinensis growth and biomass production, namely increasing the number of leaves (12%) and dry weight (35%). Also, the bacteria reduced Pb residue in the soil by up to 88%. Moreover, soil essential nutrients such as total nitrogen, available phosphorus, and exchangeable potassium increased (12%, 73%, and 200%, respectively) after the application of Pb-resistant bacteria. The bacteria have the potential for bioremediation of Pb-contaminated soils on a large scale due to the bacteria prevent Pb uptake by food crops such as B. chinensis by reducing Pb content in the soil, which is good for food safety and environmental sustainability. ","PeriodicalId":52402,"journal":{"name":"Journal of Tropical Biodiversity and Biotechnology","volume":"92 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140461695","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Safety Assessment of Bacillus subtilis G8 Isolated from Natto for Food Application 纳豆中分离出的枯草芽孢杆菌 G8 食品应用安全性评估

Journal of Tropical Biodiversity and Biotechnology Pub Date : 2024-02-02 DOI: 10.22146/jtbb.87079

Nathania Calista Putri, H. Victor, Vivian Litanto, R. Pinontoan, Juandy Jo

{"title":"Safety Assessment of Bacillus subtilis G8 Isolated from Natto for Food Application","authors":"Nathania Calista Putri, H. Victor, Vivian Litanto, R. Pinontoan, Juandy Jo","doi":"10.22146/jtbb.87079","DOIUrl":"https://doi.org/10.22146/jtbb.87079","url":null,"abstract":"Various bacteria are widely used as food-fermenting agents, including Lactobacillus, Bifidobacterium, and Bacillus. Despite they are generally recognized as safe to be consumed by humans, those bacteria could potentially cause antibiotic resistance as they could acquire and transfer antibiotic resistance genes from or to other microbes within the human gastrointestinal tract. Profiling antibiotic resistance pattern in those bacteria is therefore important to control the spread of antibiotic resistance. In this study, antibiotic resistance profile of Bacillus subtilis G8 was assessed. B. subtilis G8 had been isolated from commercialised Japanese natto in Indonesia and had been previously reported for its fibrinolytic characteristics. The antibiotic resistance phenotype and genotype of B. subtilis G8 were assessed through the Kirby-Bauer disk diffusion method and whole-genome analysis, respectively. B. subtilis G8 exhibited resistance towards Oxacillin, Lincomycin and Tiamulin-Lefamulin. The bioinformatics analysis indicated several responsible genes mediating those resistance, i.e., ybxI (for Oxacillin), lmrB (for Lincomycin) and vmlR (for Lincomycin and Tiamulin-Lefamulin). All identified genes were found in the chromosomal DNA. Further analysis found no mobile genetic elements within the genome, therefore reducing a risk of resistance gene transfer via plasmid and subsequently supporting safety profile of B. subtilis G8 in food fermentation usage.","PeriodicalId":52402,"journal":{"name":"Journal of Tropical Biodiversity and Biotechnology","volume":"175 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140462312","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Diversity of Santigi (Pemphis acidula J.R.Forst. & G.Forst.), A Mangrove Association in Tomini Bay, Sulawesi, Indonesia 印度尼西亚苏拉威西托米尼湾红树林协会 Santigi（Pemphis acidula J.R.Forst. & G.Forst.）的多样性

Journal of Tropical Biodiversity and Biotechnology Pub Date : 2024-01-29 DOI: 10.22146/jtbb.83889

D. Baderan, Sukirman Rahim, Melisnawati H. Angio, Muh. Nur Akbar, Magfirahtul Jannah, Y. Retnowati, R. Utina

{"title":"Diversity of Santigi (Pemphis acidula J.R.Forst. & G.Forst.), A Mangrove Association in Tomini Bay, Sulawesi, Indonesia","authors":"D. Baderan, Sukirman Rahim, Melisnawati H. Angio, Muh. Nur Akbar, Magfirahtul Jannah, Y. Retnowati, R. Utina","doi":"10.22146/jtbb.83889","DOIUrl":"https://doi.org/10.22146/jtbb.83889","url":null,"abstract":"Pemphis acidula is a wild plant in rocky or sandy coastal areas and mangrove ecosystems. Different geographic characteristics may affect plant adaptability and have an impact on the emergence of various genotypes. This study was performed to reveal the phenetic relationship and genetic variation of P. acidula in 3 different areas in Tomini Bay, Gorontalo Province, Indonesia. We took 3 samples from each location and analysed them using 14 morphological characters and molecular approaches based on ISSR markers and ITS gene. The results showed that P. acidula on Olele had bigger sizes in some morphological features compared to the plants in other study areas. The phenetic analysis showed that P. acidula at Biluhu and Dulanga were more closely related, although P. acidula at the 3 locations had 100% similarity. Genetic variation analysis showed the highest genetic similarity based on ISSR markers was found in Dulanga and Biluhu samples (76.8%). Phylogenetic based on ITS gene revealed that Olele samples were in the same clade with P. acidula accession from GenBank (genetic distance 0-0.19%), while Biluhu samples were a sister group (genetic distance 24.97-25.03%) even though their percentage identity corresponds to P. acidula (81.34%). Plant adaptation to different habitat conditions may affect the genetic diversity of P. acidula. ","PeriodicalId":52402,"journal":{"name":"Journal of Tropical Biodiversity and Biotechnology","volume":"39 6","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140489097","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Astaxanthin Production from Green Microalga Haematococcus pluvialis under Various Bean Sprout Media Concentrations and Duration of UV Radiations 不同豆芽培养基浓度和紫外线照射时间下绿色微藻 Haematococcus pluvialis 产生虾青素的情况

Journal of Tropical Biodiversity and Biotechnology Pub Date : 2024-01-26 DOI: 10.22146/jtbb.73763

Biaggi Rakhmat Rheinan Hary, B. R. Sidharta, I. S. Arsiningtyas

{"title":"Astaxanthin Production from Green Microalga Haematococcus pluvialis under Various Bean Sprout Media Concentrations and Duration of UV Radiations","authors":"Biaggi Rakhmat Rheinan Hary, B. R. Sidharta, I. S. Arsiningtyas","doi":"10.22146/jtbb.73763","DOIUrl":"https://doi.org/10.22146/jtbb.73763","url":null,"abstract":"Astaxanthin (AX) is known as a very strong antioxidant and has been utilised in many kinds of products such as foods, pharmaceutical, cosmetics, aquaculture, etc. One of the natural resources of AX is Haematococcus pluvialis which has been investigated by some researchers in order to enhance the AX production. However, the production of AX from the microalgae is still costly, hence, this present research is proposing low-cost methods namely bean sprout media (BSM) as an alternative growth media and UV radiation. The variations of BSM concentrations (2, 4, and 6 %) and times of UV radiation (1.5 and 3 hrs) were treated to H. pluvialis in laboratory conditions. BSM 4 % treatment showed an optimum growth of the microalga at 427 x 104 cell/ml (day 8) which also exhibited macrozooid, palmella, and aplanosore phases. UV radiation for 3 hr revealed that the concentration of AX production was as much as 17.37±0.04 mg/l. The research results were potential to be developed further in order to discover better and cheaper methods for scaling up AX production. ","PeriodicalId":52402,"journal":{"name":"Journal of Tropical Biodiversity and Biotechnology","volume":"124 12","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140494010","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Plukenetia volubilis L.: A New Record of a Cultivated Alien Species in Java Plukenetia volubilis L.：爪哇栽培外来物种的新记录

Journal of Tropical Biodiversity and Biotechnology Pub Date : 2024-01-22 DOI: 10.22146/jtbb.84523

A. Tianara, Windri Handayani, A. S. D. Irsyam, Muhammad Rifqi Hariri, A. P. Dewi, Peniwidiyanti Peniwidiyanti, Muhammad Hisyam Baidlowi, Dian Rosleine, M. Atria

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The Growth Response of Rendeu (Staurogyne elongata (Neese) Kuntze) to Shoot Pruning and Its Propagation by Shoot Cutting 莲雾（Staurogyne elongata (Neese) Kuntze）的生长对嫩枝修剪的反应以及嫩枝切条繁殖

Journal of Tropical Biodiversity and Biotechnology Pub Date : 2024-01-19 DOI: 10.22146/jtbb.77078

I. Q. Lailaty, Sri Astutik, M. I. Surya

{"title":"The Growth Response of Rendeu (Staurogyne elongata (Neese) Kuntze) to Shoot Pruning and Its Propagation by Shoot Cutting","authors":"I. Q. Lailaty, Sri Astutik, M. I. Surya","doi":"10.22146/jtbb.77078","DOIUrl":"https://doi.org/10.22146/jtbb.77078","url":null,"abstract":"Rendeu (Staurogyne elongata (Neese) Kuntze) is a native Indonesian plant used as food and traditional medicine in the daily life of the people residing around Gunung Halimun-Salak National Park. Due to the potential source of herbal-based medicines and traditional food in the long-run purposes, the proper method of its propagation is required, so that Rendeu can be conserved and utilised sustainably. This study employed two research designs. First, a completely randomized design with pruning and IAA (indole-3 acetic acid) treatment was used for seedling growths. Second, plant propagation applied a factorial randomized block design: planting media types and plant growth regulator (PGR) (rootone F) treatment. Observation included the number of buds, number of leaves, number of flowers, plant biomass, root length, and relative chlorophyll content using the SPAD tool. The data were analysed using ANOVA (SPSS ver. 17.0), followed by Pearson correlation analysis. The results showed that applying IAA and leaf pruning could increase the number of buds, the number of leaves and the fresh weight of S. elongata plants compared to the control plant. The addition of rootone increased the growth of Rendeu shoot cuttings, shown in all growth parameters and chlorophyll content. Humus was the best media for Rendeu’s growth among all planting medium. Planting media affected the increase in the number of leaves and the number of buds of S. elongata significantly. The interaction of planting media and PGR somewhat influenced root length and total leaf chlorophyll. The growth and production of S. elongata increased with the time of planting. ","PeriodicalId":52402,"journal":{"name":"Journal of Tropical Biodiversity and Biotechnology","volume":"59 7","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140502792","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0