Scanning Electron Microscopy Analysis of Tea’s Embryo Axis Explant Cultured on Murashige and Skoog Medium Containing 2,4-Dichlorophenoxyacetic acid

Q4 Agricultural and Biological Sciences

Journal of Tropical Biodiversity and Biotechnology Pub Date : 2024-02-12 DOI:10.22146/jtbb.76451

Ratna Dewi Eskundari, Taryono Taryono, D. Indradewa, Y. A. Purwestri

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Abstract

Camellia sinensis L. is an important crop in Indonesia as healthy beverage that contains several secondary metabolism compounds, such as polyphenols and catechins. Tissue culture including somatic embryogenesis and organogenesis has been used for propagating plant for various needs. In this present short-communication, scanning electron microscopic (SEM) analysis of tea was conducted and discussed. This study aimed to investigate surface ultrastructure of TRI2025 embryo axis tea clone cultured on Murashige and Skoog (MS) medium containing 2,4-Dichlorophenoxyacetic acid (2,4-D). The results revealed two different forms of explant’s development, i.e. somatic embryo and transitional form between somatic embryogenesis and organogenesis; or called by “Globular-like Structure” (GLS). Surface ultrastructure analysis of somatic embryo and GLS revealed respectively many stages of somatic embryo development i.e. globular, torpedo, and cotyledon stage, and leaf development form GLS regeneration.

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在含有 2,4-二氯苯氧乙酸的 Murashige 和 Skoog 培养基上培养的茶树胚轴外植体的扫描电子显微镜分析

山茶花是印度尼西亚的一种重要作物，作为健康饮料，它含有多种次级代谢化合物，如多酚和儿茶素。组织培养包括体细胞胚胎发生和器官发生，已被用于繁殖植物以满足各种需要。本简讯对茶叶进行了扫描电子显微镜（SEM）分析和讨论。本研究旨在调查在含有 2,4-D 的 Murashige 和 Skoog（MS）培养基上培养的 TRI2025 胚轴茶克隆的表面超微结构。结果发现了两种不同的外植体发育形式，即体细胞胚胎和介于体细胞胚胎发生与器官发生之间的过渡形态，或称为 "球状结构"（GLS）。对体细胞胚和 GLS 的表面超微结构分析表明，体细胞胚的多个发育阶段（即球状、鱼雷状和子叶阶段）和 GLS 再生的叶片发育阶段都是如此。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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