Revista Bionatura最新文献

{"title":"Studying the Genetic Parameters of Some Cultivars of Faba Bean (Vicia faba L.) and Evaluating Their Performance under Different Levels of Phosphorous","authors":"Ali H. Algraishi, Faez F. Alogaidi","doi":"10.21931/rb/css/2023.08.03.78","DOIUrl":"https://doi.org/10.21931/rb/css/2023.08.03.78","url":null,"abstract":"A field experiment was carried out during the winter season of 2021-2022 in the research station - College of Agricultural Engineering Sciences - University of Baghdad in order to study the genetic parameters of some cultivars of faba bean cultivars and evaluate their performance under different levels of phosphorus. Spilled plots were arranged according to randomized complete block design (RCBD) at three replications. The main plots included three levels of phosphorous fertilizer (80, 120 and 160 Kg ha-1 ) and the control treatment (without fertilizer). In contrast, the subplots included four faba bean cultivars (Local, Spanish, Dutch and New Zealand). The results showed highly significant effects of phosphorous fertilizer levels on the most studied traits, as adding phosphorous fertilizer at 160 Kg gave the highest means of several pods per plant (17.78 pods plant-1 ) and seed yield (4.403 tons ha-1 ). Also, the results showed that the faba bean cultivars were significantly different in most studied traits, as the Local cultivar achieved the highest mean number of pods per plant (16.95pod plant-1 ). In contrast, the Dutch cultivar achieved the highest mean number of seeds per pod (5.78 seed pod-1 ), whereas the Spanish cultivar achieved the highest mean weight of 100 seeds (86.95 g) and seed yield (5.295 ton ha-1 ). The interaction between studied factors had a non-significant effect on all studied traits except the number of pods per plant. The genetic variance recorded greater values than the environmental variance in most of the studied traits, indicating that the genetic variance significantly contributed to the phenotypic variance. Keywords: Genetic indicators, phosphorous, Vicia faba L., G.C.V., PCV.","PeriodicalId":52287,"journal":{"name":"Revista Bionatura","volume":"13 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135761492","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The expression miRNA-195 in a sample of Iraqi breast cancer patients.","authors":"Ahmed Nasser Ibraheem AL-saqabi, Ismail Hussein Aziz","doi":"10.21931/rb/css/2023.08.03.87","DOIUrl":"https://doi.org/10.21931/rb/css/2023.08.03.87","url":null,"abstract":"Breast carcinoma is the most prevalent cancer-related cause of death in women, and metastasis is the main factor in morbidity. The total number of new cases of cancer in Iraq during the year 2019 was 35,864. New non-invasive prognostic biomarkers are needed for the rapid recognition and differentiation between breast cancer (BC) stages for treatment choice improvement. MicroRNA (miRNA) are small, noncoding RNAs regulating gene expression and involve many cellular processes, including metastasis. Circulating miRNAs (detected in the blood) show considerable potential as biomarkers for helping diagnosis or tracking treatment efficacy. Materials and Methods: total RNA was extracted from serum from (n=50) patients and (n=26) healthy control to measure the MicroRNA 195 expression using SYBR green-based real-time RT-PCR technology. As a result, the expression levels miR-195 in breast cancer patients’ serum were significantly increased (up-regulated) compared to those in the normal adjacent serum. BC group showed a higher significant miR-195 expression (upregulation) when compared with those in the control group. While the highest expression of miR195 was recorded in stage II. Keywords: Breast Cancer, MicroRNA, miR-195, Stage, Iraq","PeriodicalId":52287,"journal":{"name":"Revista Bionatura","volume":"16 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135761494","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"FSCN1 Gene Expression Influence on Breast Cancer in Iraqi Patients","authors":"Rifaat M. Rifaat, Mohammed I. Nader","doi":"10.21931/rb/css/2023.08.03.85","DOIUrl":"https://doi.org/10.21931/rb/css/2023.08.03.85","url":null,"abstract":"Our investigation aim was to ascertain how the Fascin actin-bundling protein 1 (FSCN1) gene affected breast cancer patients and how it affected the molecular type of the disease (Luminal A, Luminal B, Triple negative, Enrich Her2).By collecting blood samples from sixty patients with Breast cancer from many hospitals in Baghdad/Iraq between the periods (October 2021 to August 2022) and collecting forty volunteers heaths as Control. The volunteer's blood samples were collected and tested with Cancer antigen 15-3 (CA 15-3) to confirm absent breast cancer. The blood and control samples from these patients were collected, the RNA was extracted, and molecular methods using PCR and primers targeting the FSCN1 gene were used. The results showed that genes found in all patient samples have overexpression compared to healthy volunteers, and we also discovered differences in expression between the molecular types of breast cancer. We discovered that Triple-negative breast cancer is more affected than Luminal A, B, and Enrich Her2. Keywords: Breast Cancer, Molecular classification breast cancer, Fascin Actin Bundling protein 1 genes","PeriodicalId":52287,"journal":{"name":"Revista Bionatura","volume":"72 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135761501","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Autophagy induction using Resveratrol enhances the anti-cancer efficacy of Doxazosin in breast cancer cells.","authors":"Ali Sami Ahmed Alhayali, Waseem Ali Hasan, Firas Subhi Salah","doi":"10.21931/rb/css/2023.08.04.88","DOIUrl":"https://doi.org/10.21931/rb/css/2023.08.04.88","url":null,"abstract":"The combination of anti-cancer drugs improves effectiveness compared to the mono-therapy scenario by targeting key pathways synergistically or in an additive way. Doxazosin (DOX) and Resveratrol (RES) are reported to have an anti-cancer impact against different cancer cells. Aim: To evaluate the anti-cancer properties of Doxazosin and Resveratrol, each alone or in combination, in inhibiting breast cancer cell proliferation. Methods: MCF-7 cancer cells were seeded to a confluent monolayer and treated with 100, 50, 25, 12.5, 6.25, and 3.12 µM of each drug alone and as a combination. Cytotoxicity was evaluated using dimethyl thiazolyl diphenyl tetrazolium salt (MTT) assay and colony formation assay. The immunocytochemistry technique was conducted to evaluate caspase-3 and GABARAP expression. Results: All the drugs have a significant effect on cancer cell proliferation. The combination treatment of Doxazosin-Resveratrol has a more cytotoxic effect than each drug alone, as well as in colony formation of MCF-7 cancer cells. In combination treatment, caspase-3 had a higher expression pattern than other treatments. Resveratrol treatment elevated GABARAP expression in cancer cells, indicating the induction of the autophagy process. Conclusion: Although each drug has its characteristic result of a cytotoxic effect, the combination of Doxazosin and Resveratrol synergizes the inhibition of MCF-7 cancer cell proliferation. Keywords: Resveratrol; Doxazosin; combination; MCF-7; Autophagy.","PeriodicalId":52287,"journal":{"name":"Revista Bionatura","volume":"42 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136184328","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synthesis, Characterization and Evaluation of the Biological Activity of Some New Thiazolidine Derivatives Derived from Schiff bases","authors":"Hussam Z. Naji, Eman M. Hussain","doi":"10.21931/rb/css/2023.08.03.20","DOIUrl":"https://doi.org/10.21931/rb/css/2023.08.03.20","url":null,"abstract":"In this research, new compounds were synthesized via the reaction of dichloroacetic acid with two moles of piperidine. The novel acid 1 was converted to its ester 2. Acid hydrizide 3 was prepared by the reaction of hydrazine hydrate with new ester 2, which was later used to prepare derivatives of Schiff bases 4-13. In the last step, Schiff bases and thioglycolic acid were reacted to give thiazolidine derivatives 14-23. All these compounds were diagnosed using melting points, FTIR, 1HNMR and mass spectroscopy. Scheme 1 shows all the synthesized compounds' reaction steps and structures. Keywords: Thiazolidine; Schiff bases; biological activity; piperidine; dichloroacetic acid.","PeriodicalId":52287,"journal":{"name":"Revista Bionatura","volume":"2011 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135760110","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A comparison with micro albuminuria indicates whether urine and serum kidney injury molecule-1 can predict the onset of early diabetic nephropathy (DN).","authors":"Dalia M. Saleh, Manal K Rasheed, Nabeel K. Alwandi","doi":"10.21931/rb/css/2023.08.03.46","DOIUrl":"https://doi.org/10.21931/rb/css/2023.08.03.46","url":null,"abstract":"Diabetic nephropathy (DN) is the most frequent consequence of DM and a significant contributor to chronic kidney disease, a multifactorial illness. Kidney injury molecule-1 (KIM-1) is a sensitive and accurate indicator of kidney damage and a predictive indicator. Aim: The present investigation aimed to examine serum and urine KIM-1 as a DN early marker. Patients and methods: The present study included 150 participants, among whom 50 non-diabetic participants were chosen as controls. One hundred diabetic participants were split into two groups based on their urine albumin/creatinine ratio (ACR) as participants with normoalbuminuria (T2DM patients without nephropathy) and microalbuminuria (T2DM patients with nephropathy). The blood glucose, HbA1c, s.urea, and creatinine levels in serum and urine were measured using standard laboratory techniques, and Elisa Essay measured serum and urine KIM-1 levels. Results: There was a distinct variation in the mean serum and urine KIM-1 between the control and diabetics without microalbuminuria (P = 0.001). Serum KIM-1 correlated with ACR (P = 0.669) in people with diabetes with microalbuminuria. Urine KIM-1 was less correlated than serum KIM-1 (p = 0.257). A strong association was found between ACR and serum KIM1 in people with diabetes with microalbuminuria and a low correlation between ACR and urine KIM-1 in people with diabetes with microalbumin. Conclusion: According to the current investigation, diabetic groups with microalbuminuria had higher serum and urine levels of KIM-1 than the control groups. Additionally, there was a favorable correlation between serum KIM-1 and the length of diabetes. More extensive multicentric trials are also needed to assess the efficacy of serum and urine KIM-1 as a DN early marker. Keywords: Serum /Urine KIM-1; Dibetic nephropathy; Microalbumin; DM type2","PeriodicalId":52287,"journal":{"name":"Revista Bionatura","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136183704","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Response of Local Garlic to Potassium and Sulfur Fertilization Method","authors":"Raed K. A. Al-Mohammadi, Maath M. M. Al-Abdaly","doi":"10.21931/rb/css/2023.08.04.11","DOIUrl":"https://doi.org/10.21931/rb/css/2023.08.04.11","url":null,"abstract":"A field experiment was conducted at the Agricultural Research Station of the College of Agriculture, University of Anbar, for the agricultural season 2021- 2022. This experiment aimed to study the effect of potassium and sulfur application on local garlic's growth, yield, and quality. The experiment included four levels of potassium soil fertilization (0, 200, 250, and 300 kg.ha-1 ), three levels of foliar spraying of potassium (0, 5, and 10 g.L-1 ), and three levels of soil fertilization of sulfur (0, 40, and 50 kg.ha-1 ). The study used the randomized complete block design (RCBD) in combinations. The results showed significant differences in the characteristics of vegetative growth, yield characteristics, and qualitative characteristics. The combination (300 kg K2O.ha-1 +10 g.L-1 + 50 kg S.ha-1 ) significantly exceeded by giving the highest leaves number, leaves area and shoot dry weight, which amounted to 11.42 leaf.plant-1 and 795.00 cm2 .plant-1 and 10.87 g.plant-1 , respectively. In comparison, the control treatment gave (8.80 leaves.plant-1 and 571.00 cm2 .plant-1 and 8.17 g.plant-1 ), for the same above traits, respectively. The same combination achieved a significant increase in yield traits (cloves number, clove weight, yield/plant, and total yield) with 37.58 cloves, 2.71 g, 102.03 g, and 51.01 t.ha-1 , respectively, compared to the control treatment, which gave (32.00 cloves, 1.81 g, 58.17 g, and 29.08 t.ha-1 ). The same treatment led to an increase in the percentage of potassium and sulfur in the bulbs, which recorded the highest percentage of 1.67% and 0.60%, respectively, compared to the control treatment, which recorded the lowest percentage of 1.36% and 0.40%, respectively. Keywords: Garlic, Fertilization, Potassium, Sulfur, Growth, Yield.","PeriodicalId":52287,"journal":{"name":"Revista Bionatura","volume":"179 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136184011","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of Adding Levels of Palm Fronds Compost and Mineral Fertilizer on Fertile Soil Characteristics, Quality, and Productivity of Maize Yield (Zea Mays L.)","authors":"Abeer Mounir Abdel Hadi, Bushra Mahmoud Alwan","doi":"10.21931/rb/css/2023.08.03.29","DOIUrl":"https://doi.org/10.21931/rb/css/2023.08.03.29","url":null,"abstract":"An experiment was carried out in one of the fields of the Soil Research Department - Agricultural Research Department/Ministry of Agriculture in the Abu Ghraib district at latitude 33° 17\" 31 N and longitude 44° 03\" 56 E and 35 m above sea level. This field study was conducted in the autumn season 2021-2022 for the cultivation of yellow maize ( Maha cultivar) in sedimentary soil with silty clay loam texture, with a Split Plot Design. The treatments were distributed using the RCBD for three replicates with two factors. The first factor represents the main plots that include the addition of mineral fertilizer 120, 80, and 320 kg.ha-1 for each of (N, P, and K), respectively, at three levels (0, 50, and 100%) of the fertilizer recommendation. On the other hand, the second factor was a subplot of organic fertilizer (palm frond compost) at three levels (0, 5, and 10) μg.ha-1 . Soil samples were taken to estimate the availability of nitrogen, phosphorous, and potassium, where the total yield and percentage of protein for yellow maize were estimated. The results showed that adding organic fertilizers (palm fronds) with the levels of mineral fertilizers increased the availability of nutrients and the yield of maize. Accordingly, the treatment O2M2 achieved the highest values of nutrient availability, quantity, and quality of yield, reaching (59.167, 33.407, and 257.967) mg.kg-1 soil and (10,096) μg.ha-1 and (9.435%) for each of nitrogen, phosphorous, potassium, yield, and protein percentage, respectively. Moreover, there were no significant differences between the treatments of O1M2 and O2M1 in the amount of yield and the percentage of protein, which gave the values of 8.403 and 8.134 μg.ha-1, respectively, and the protein percentage of 8.630 and 8.915% respectively. It can be concluded that organic fertilizers can be added to compensate for mineral fertilizers, reduce environmental pollution, and reduce the economic cost and soil preservation because organic fertilizers are considered safe and environmentally friendly. Keywords: yellow maize, mineral fertilizer, palm fronds compost.","PeriodicalId":52287,"journal":{"name":"Revista Bionatura","volume":"80 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136184318","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Minimizing the side effects of Doxorubicin Induced Hepatotoxicity by using alcoholic extract of Date Palm in adult rats","authors":"Najwa Imad Sulaiman Saleh, Siham Agmee Wadee, Entedhar R. Sarhat","doi":"10.21931/rb/css/2023.08.04.86","DOIUrl":"https://doi.org/10.21931/rb/css/2023.08.04.86","url":null,"abstract":"Doxorubicin (DOX) is a highly effective drug for chemotherapy. However, hepatotoxicity reduces its clinical utility in humans. Thus, this study was designed to examine Date Palm extract on serum anti-inflammatory markers (interleukin(IL) IL-1B, IL-6 and IL-10). Forty adult rats were divided into 4 groups (G1 control, G2 receiving 2mgkg of DPE orally, G3 treated with 2mgkg of DOX IP, and G4 received 2mgkg of DOX via IP and 2mgkg of DPE by oral gavage daily for 30 days). At the end of the study, animals were sacrificed, and livers were analyzed histologically. The Dox group showed significantly higher levels of serum IL-1B, IL-6, and IL-10 than the control group, with inflammation and necrosis in hepatic histopathology. In the DPE+ DOX group, it was detected that DOX treatment caused a significant decrease in serum IL-1B, IL-6, and IL-10 levels. Collectively, pre-coadministration of DPE partially mitigated DOX-induced hepatic injuries via its antioxidant, anti-inflammatory, anti-fibrotic, and antiapoptotic protein.","PeriodicalId":52287,"journal":{"name":"Revista Bionatura","volume":"77 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136184324","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Estimation of Some Biochemical Tests in the Serum of Obese Men with Normal Blood Pressure and High Blood Pressure","authors":"Shaimaa Khalid Moufak, Rowshen Hani Al Nakeeb, Tamara Sami Naji","doi":"10.21931/rb/css/2023.08.04.25","DOIUrl":"https://doi.org/10.21931/rb/css/2023.08.04.25","url":null,"abstract":"Obesity is a medical word that refers to a state in which a person's body fat levels are excessively high. Obesity is linked to a variety of health issues in humans, like the emergence of hypertension. This study aimed to look at the blood glucose concentration, urea, creatinine, and lipid profile factors in obese and hypertension-overweight men's sera. The study included 75 people who were evenly divided into three groups: healthy controls, normotensive obese people, and hypertension-obese people. When comparing obese males to controls, there was a significant increase (P<0.01) in blood glucose, urea, creatinine, cholesterol, triglycerides, low-density lipoprotein cholesterol (LDL-C), and very low-density lipoprotein cholesterol (VLDL-C) although only triglycerides (TGs) and VLDL-C were substantially different in hypertension obese versus normotensive obese. Compared to controls, Obese men had significantly lower levels of high-density lipoprotein cholesterol (HDL-C). High levels of triglycerides (TGs) and Very Low-density Lipoprotein cholesterol (VLDL) in hypertensive obese males show they play a role in hypertension problems. Keywords: Obesity, Hypertension, Urea, Creatinine, lipid profile.","PeriodicalId":52287,"journal":{"name":"Revista Bionatura","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136183127","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}