{"title":"Surface roughness and its role in mediating cell adhesion on cobalt-chromium-molybdenum alloys","authors":"Satoshi Migita","doi":"10.1049/bsb2.12068","DOIUrl":"10.1049/bsb2.12068","url":null,"abstract":"<p>Co-Cr-Mo ally (CCM) is commonly used for orthopaedic and dental implants due to its excellent mechanical properties and corrosion resistance. However, the influence of surface roughness on cell attachment and proliferation remains unclear. This study aimed to elucidate the impact of surface roughness of CCM on the attachment and proliferation of osteoblasts. CCM samples with different values of surface rouges were prepared by polishing. MC3T3-E1 mouse osteoblasts were used for cell culture experiments. Cell attachment, morphology, and the expression of actin stress fibres, vinculin, and distribution of yes-associated protein were analysed. Our results suggest that surface roughness does not significantly affect cell attachment and proliferation on CCM, unlike on titanium. Thus implies that other properties of CCM, such as physicochemical properties, may play a more substantial role in modulating cell behaviour. This study provides important insights into the design of CCM implants, suggesting that approaches beyond tuning surface roughness may be necessary to improve biocompatibility and osseointegration.</p>","PeriodicalId":52235,"journal":{"name":"Biosurface and Biotribology","volume":"9 4","pages":"161-168"},"PeriodicalIF":1.6,"publicationDate":"2023-11-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1049/bsb2.12068","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135141765","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Polyphenol-mediated construction of highly stable and bioactive selenium nanoparticles","authors":"Rong Hu, Xiao Wang, Lu Han, Xiong Lu","doi":"10.1049/bsb2.12067","DOIUrl":"10.1049/bsb2.12067","url":null,"abstract":"<p>Selenium (Se), a well-known essential element in human health, plays a vital role in regulating metabolism owing to its antioxidative nature. However, organic Se compounds are toxic and cannot be used for biomedical applications. Selenium nanoparticles (SeNPs) exhibit low biological toxicity and high bioavailability; however, they are prone to aggregation and are extremely unstable, thereby diminishing their bioactivity and bioavailability. To overcome these limitations, ultra-small, highly stable, and bioactive SeNPs were synthesised based on an in-situ hybridisation strategy by using polyphenol-grafted-chitosan (GA-CS) to control and restrict crystal growth of Se nanoparticles. The resultant GA-CS@nSe exhibited an average particle size of ∼30 nm and was highly stable in aqueous solutions. In addition, GA-CS@nSe displayed improved biocompatibility and enhanced antioxidative activity. Taken together, the authors provide a basis for polyphenol-mediated construction of Se-based particles with increased bioactivity.</p>","PeriodicalId":52235,"journal":{"name":"Biosurface and Biotribology","volume":"9 4","pages":"151-160"},"PeriodicalIF":1.6,"publicationDate":"2023-10-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1049/bsb2.12067","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135779990","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Tuo Qin, Aibing Yu, Shuo Zhao, Kefan Li, Shaochun Qi, Jiawang Ye
{"title":"Tribological characteristics of dimpled surfaces filled with dopamine-modified MoS2","authors":"Tuo Qin, Aibing Yu, Shuo Zhao, Kefan Li, Shaochun Qi, Jiawang Ye","doi":"10.1049/bsb2.12066","DOIUrl":"10.1049/bsb2.12066","url":null,"abstract":"<p>To improve the tribological characteristics of dimples on the surface of 45 steel, the dimples were filled with MoS<sub>2</sub> and MoS<sub>2</sub> modified by dopamine (MoS<sub>2</sub> @ DA), and ball-disk friction and wear tests were conducted. Specifically, the dimple filling gap, abrasion depth, and surface cross-sectional area of 45 steel were measured. The wear morphology of the friction ball and exfoliation of MoS<sub>2</sub> in the dimples and the bending characteristics of the specimens were studied. The surface friction coefficient of MoS<sub>2</sub> @ DA-filled specimen was 17.9% lower than MoS<sub>2</sub>-filled specimen, and the dimple filling gap was 70.1% lower, the surface abrasion depth was 5.8% lower, and the abrasion cross-sectional area was 17.7% smaller. Moreover, the bending strength of the MoS<sub>2</sub> @ DA specimen was 3.27 times greater than that of the MoS<sub>2</sub> specimen, and the exfoliation of MoS<sub>2</sub> was slowed by filling with the MoS<sub>2</sub> @ DA. Finally, the tribological characteristics were also superior for the specimens prepared with MoS<sub>2</sub> @ DA.</p>","PeriodicalId":52235,"journal":{"name":"Biosurface and Biotribology","volume":"9 4","pages":"142-150"},"PeriodicalIF":1.6,"publicationDate":"2023-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1049/bsb2.12066","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136034355","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Multifunctional hydrogels for chronic wounds repairing","authors":"Xiao Wang, Rong Hu, Lu Han, Xiong Lu","doi":"10.1049/bsb2.12064","DOIUrl":"10.1049/bsb2.12064","url":null,"abstract":"<p>Because of their tissue-like mechanical performances, high biocompatibility, and adjustable functionality, hydrogels have become increasingly attractive materials for promoting wound healing. Chronic wounds include burn, diabetic, and infected wounds. Unlike common incision wounds, chronic wounds are more challenging to heal. To meet the clinical needs, multifunctional hydrogels should be fabricated and investigated. To guide future studies on the fabrication of hydrogel-based chronic wound dressings, a review of advanced multifunctional hydrogels is necessary. Various hydrogels with advanced properties, such as antibacterial, antioxidant, bioadhesive, anti-inflammatory, and wound healing properties, that can be used for skin burn wounds and diabetic wounds are summarised. Lastly, the prospects of advanced hydrogels for wound healing are elaborated.</p>","PeriodicalId":52235,"journal":{"name":"Biosurface and Biotribology","volume":"9 4","pages":"85-100"},"PeriodicalIF":1.6,"publicationDate":"2023-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1049/bsb2.12064","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136115911","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Tao Chai, Hao Zhang, Xiaolong Shen, Haibo Wang, Xingping Fan, Binbin Wang, Dingying He, Jia Su
{"title":"A polyphenol-induced hydroxyapatite coating modulates corrosion resistance and biocompatibility of magnesium alloys","authors":"Tao Chai, Hao Zhang, Xiaolong Shen, Haibo Wang, Xingping Fan, Binbin Wang, Dingying He, Jia Su","doi":"10.1049/bsb2.12065","DOIUrl":"10.1049/bsb2.12065","url":null,"abstract":"<p>In order to solve the problem of excessive degradation rate and insufficient biocompatibility of magnesium-based bone implants, a polyphenol (EGCG) induced hydroxyapatite (HA) coating was prepared on the surface of AZ31 alloy. The physical and chemical properties and corrosion resistance of the coating were analysed in depth, and its biocompatibility was preliminarily explored in vitro. The results showed that the polyphenol (EGCG) conversion coating constructed on the AZ31 could successfully induce the formation of HA by complexing the phenolic hydroxyl group with calcium ions. The electrochemical and long-term immersion experiments showed that the corrosion resistance of EGCG/HA composite coating was significantly improved. The self-corrosion current density, hydrogen evolution and the increase of pH value of AZ31-EGCG/HA were significantly lower than those of AZ31. On the basis of inhibiting the excessive corrosion of the substrate, the composite coating significantly improves the compatibility of pre-osteoblasts, supports the adhesion and spreading and effectively reduces the haemolysis rate to less than 5%. The preparation method of the coating is simple, low cost and suitable for complex shape surfaces, which can significantly improve the corrosion resistance and biocompatibility of the AZ31 substrate. It is expected to provide a solution for the surface modification of magnesium-based bone implants.</p>","PeriodicalId":52235,"journal":{"name":"Biosurface and Biotribology","volume":"9 4","pages":"129-141"},"PeriodicalIF":1.6,"publicationDate":"2023-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1049/bsb2.12065","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135147220","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Bin He, Chen Wang, Xiong Xiong, Jun Li, Zhongmin Jin, Shuxin Qu
{"title":"Bio-inspired low wear and durable lubrication interfacial system based on thixotropic hydrogel for artificial joints","authors":"Bin He, Chen Wang, Xiong Xiong, Jun Li, Zhongmin Jin, Shuxin Qu","doi":"10.1049/bsb2.12063","DOIUrl":"10.1049/bsb2.12063","url":null,"abstract":"<p>Inspired by the excellent wear resistance and lubrication of articular joints, a novel bionic interfacial system was proposed by combining thixotropic hydrogel with surface porous Ultrahigh Molecular Weight Polyethylene (UHMWPE). Thixotropic hydrogel, synthesised by gelatin, alginate sodium, tannic acid and weak crosslinking by Ca<sup>2+</sup> (Gel-TA-Alg@Ca<sup>2+</sup>), was used as a lubricant due to its shear-thinning when loaded, then the recovery viscosity to be benefitted for reserving in surface pores on UHMWPE when unloaded. Surface porous UHMWPE was fabricated by using hydroxyapatite particles as porogen to control its porosity, pore size, surface roughness and surface energy (PE-HA). Gel-TA-Alg@Ca<sup>2+</sup> significantly reduced average coefficients of friction and wear factors compared to those under normal saline and calf serum solution lubricating after reciprocating tribological testing. Notably, Gel-TA-Alg@Ca<sup>2+</sup> still maintained thixotropy and was stored in surface pores of UHMWPE even after tribological testing for 7200 min. Thus, durable lubrication could be realised due to the synergistic effect of surface porous structure and thixotropy. Stribeck curves showed the characterisations of mixed, elastohydrodynamic and hydrodynamic, but without boundary lubrications for PE-30HA under three lubricants. The present results might provide the potential application to construct the durable lubrication bionic articular joint interfacial system for artificial joints.</p>","PeriodicalId":52235,"journal":{"name":"Biosurface and Biotribology","volume":"9 3","pages":"59-70"},"PeriodicalIF":0.0,"publicationDate":"2023-09-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1049/bsb2.12063","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43302131","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Raelene M. Cowie, Adam Briscoe, Louise M. Jennings
{"title":"The influence of lubricant temperature on the wear of total knee replacements","authors":"Raelene M. Cowie, Adam Briscoe, Louise M. Jennings","doi":"10.1049/bsb2.12061","DOIUrl":"10.1049/bsb2.12061","url":null,"abstract":"<p>Experimental in vitro simulation can be used to predict the wear performance of total knee replacements. The in vitro simulation should aim to replicate the in vivo loading, motion and environment experienced by the joint, predicting wear and potential failure whilst minimising test artefacts. Experimental wear simulation can be sensitive to environmental conditions; the environment temperature is one variable which should be controlled and was the focus of this investigation. In this study, the wear of an all-polymer (PEEK-OPTIMA™ polymer-on-UHMWPE) total knee replacement and a conventional cobalt chrome-on-UHMWPE implant of similar initial surface topography and geometry were investigated under elevated temperature conditions. The wear was compared to a previous study of the same implants under simulator running temperature (i.e. without heating the test environment). Under elevated temperature conditions, the wear rate of the UHMWPE tibial inserts was low against both femoral component materials (mean <2 mm<sup>3</sup>/million cycles) and significantly lower (<i>p</i> < 0.05) than for investigations at simulator running temperature. Protein precipitation from the lubricant onto the component articulating surfaces is a possible explanation for the lower wear. This study highlights the need to understand the influence of different variables including environmental temperature to minimise the test artefacts during wear simulation which may affect the wear rates.</p>","PeriodicalId":52235,"journal":{"name":"Biosurface and Biotribology","volume":"9 3","pages":"71-77"},"PeriodicalIF":0.0,"publicationDate":"2023-09-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1049/bsb2.12061","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45349423","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Xu Ye, Masashi Yamazaki, Wataru Ando, Norimasa Nakamura, Hiromichi Fujie
{"title":"Morphology and gene expression of sex-determining region Y-box 9 and runt-related transcription factor 2 in centrifugally compressed cell collagen-combined constructs (C6)","authors":"Xu Ye, Masashi Yamazaki, Wataru Ando, Norimasa Nakamura, Hiromichi Fujie","doi":"10.1049/bsb2.12062","DOIUrl":"10.1049/bsb2.12062","url":null,"abstract":"<p>The authors previously developed a scaffold-free tissue-engineered construct (TEC) from mesenchymal stem cells (MSCs). Although the TEC exhibited even cell distribution and was successfully applied for cartilage repair in animal models, it is unsuitable for relatively large-scale cartilage defects due to its small size. To solve the problem, the authors recently developed a novel biomaterial, a centrifugally compressed cell-collagen combined construct (C<sup>6</sup>) from a mixture of MSCs and atelocollagen, both of which are subjected to centrifugation. The results of the previous study indicated that C<sup>6</sup> exhibited high cell viability (70 %) and sufficient cell distribution similar to that of the TEC. In the present study, the morphology and gene expression of C<sup>6</sup> were investigated. Histological examination indicated that C<sup>6</sup> is six times thicker (approximately 1 mm) than the TEC after a 7-day culture. The C<sup>6</sup> remained unchanged in scale with increased cell density after a 21-day culture. Scanning electron microscopic observation indicated that C<sup>6</sup> exhibited interconnected and porous microstructures, while the TEC had close-knit microstructures. Reverse transcriptase-polymerase chain reaction analysis indicated that the expression of sex-determining region Y-box 9 and runt-related transcription factor 2 was significantly higher in C<sup>6</sup> than that in TEC.</p>","PeriodicalId":52235,"journal":{"name":"Biosurface and Biotribology","volume":"9 3","pages":"78-83"},"PeriodicalIF":0.0,"publicationDate":"2023-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1049/bsb2.12062","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48074706","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Zhiyong Li, Lu Zhang, Lei Zhou, Xin Li, Yuancong Zhao, Jin Wang
{"title":"Tunable catalytic release of nitric oxide via copper-loaded coatings on titanium nanotubes for regulating biological performance","authors":"Zhiyong Li, Lu Zhang, Lei Zhou, Xin Li, Yuancong Zhao, Jin Wang","doi":"10.1049/bsb2.12060","DOIUrl":"10.1049/bsb2.12060","url":null,"abstract":"<p>Severe lesions in vessels need to be treated with implantable interventional devices such as vascular stents, which should be anti-coagulantion, anti-proliferation and promoting endothelialisation. Nitric oxide (NO), as a physiological gas signalling molecule, play an important role in revascularisation. Catalysing the release of NO from endogenous donors has already been widely favoured to treatment strategy for lesioned vessels. In this work, a series of copper-loaded coatings (titanium nanotube (TNT)/PDA-Cu) was fabricated by TNTs combined with polydopamine and ions, which achieve controlled in situ catalytic release of NO. This strategy could effectively immobilised copper ions on TNTs, and promoted the proliferation of endothelial cells and inhibited growth of smooth muscle cells (SMCs) via the performance of NO, as well as restrain the platelet adhesion. With the multiple function, TNT/PDA-Cu provides a promise approach for promoting endothelialisation, anti-coagulation and inhibition of SMC proliferation via copper-loaded coatings on TNTs.</p>","PeriodicalId":52235,"journal":{"name":"Biosurface and Biotribology","volume":"9 2","pages":"45-57"},"PeriodicalIF":0.0,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1049/bsb2.12060","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46826029","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Yu Zhu, Lu Zhang, Lei Zhou, Xin Li, Yuancong Zhao, Jin Wang
{"title":"Investigation on baicalin-loaded chitosan film crosslinked by graphene oxide and its biocompatibility","authors":"Yu Zhu, Lu Zhang, Lei Zhou, Xin Li, Yuancong Zhao, Jin Wang","doi":"10.1049/bsb2.12059","DOIUrl":"10.1049/bsb2.12059","url":null,"abstract":"<p>Chitosan has good biocompatibility, in vivo biodegradability and certain physiological activity, which can be used as a drug carrier to stabilise and protect drug components, causing the promotion of drug absorption and controlling drug release. Using graphene oxide (GO) as a cross-linking agent, the functional groups in its lamellar structure can interact effectively with the functional groups in chitosan molecules, leading to enhanced mechanical properties and optimize the controlled drug-release behaviour. GO/chitosan composite films were prepared by the solution mixing method with various GO content and exhibit many hydrogen bonds between the GO and chitosan. There were no obviously agglomerated GO particles in the surface of composite films, and the lamellar structure can be observed in the cross section of the composite films. Differential thermal analysis (DTA) indicated that the addition of GO increased the stability of the chitosan film. The baicalin-loaded GO/Chitosan also exhibited pH dependent release behaviour, with an increasing release amount and rate at lower pH medium. In addition, the result of biological evaluations showed that the GO/Chitosan films had good cytocompatibility in vitro and histocompatibility at appropriate GO concentrations. This investigation offered a new pH-dependent drug carrier potentially applied for tumour and atherosclerosis treatment.</p>","PeriodicalId":52235,"journal":{"name":"Biosurface and Biotribology","volume":"9 2","pages":"35-44"},"PeriodicalIF":0.0,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1049/bsb2.12059","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47217496","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}