Zeitschrift fur Naturforschung. C, Journal of biosciences最新文献

{"title":"Root essential oil of <i>Chrysopogon zizanioides</i> relaxes rat isolated thoracic aorta - an <i>ex vivo</i> approach.","authors":"Lekha Sivakumar,&nbsp;David Raj Chellappan,&nbsp;Venkatraman Sriramavaratharajan,&nbsp;Ramar Murugan","doi":"10.1515/znc-2020-0143","DOIUrl":"https://doi.org/10.1515/znc-2020-0143","url":null,"abstract":"<p><p>Root of <i>Chrysopogon zizanioides</i> (L.) Roberty has been used in Siddha system of medicine to treat hypertension. The present study was therefore to investigate the vasorelaxation effect of root essential oil of <i>C. zizanioides</i> using rat isolated thoracic aortic rings. Chemical characterization of root essential oil was carried out using Gas Chromatography-Flame Ionization Detector (GC-FID) and Gas Chromatography-Mass Spectrometry (GC-MS). Essential oil nanoemulsion (EONE) was prepared and characterized. Vasorelaxant effect of EONE in endothelium-intact aortic rings precontracted with phenylephrine (PE) (1 µM) or KCl (80 mM) was investigated. Role of Ca²⁺, nitric oxide and K⁺ channels in precontracted aortic rings were investigated to elucidate the mechanism of action of the essential oil. Further, the role of muscarinic and prostacyclin receptors in EONE induced relaxation was studied. The EONE significantly induced relaxation (E_max 77.1 ± 4.87%) in PE precontracted aortic rings. The nitric oxide synthase, and cyclooxygenase inhibitors and potassium channel blockers have not significantly inhibited the vasorelaxation induced by EONE. However, EONE induced relaxation in precontracted endothelium-intact aortic rings was significantly inhibited by muscarinic receptor and calcium channel. The root essential oil of <i>C. zizanioides</i> possesses vasorelaxant effect through muscarinic pathway as well as acts as calcium channel blocker.</p>","PeriodicalId":520830,"journal":{"name":"Zeitschrift fur Naturforschung. C, Journal of biosciences","volume":" ","pages":"161-168"},"PeriodicalIF":2.0,"publicationDate":"2020-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1515/znc-2020-0143","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38578274","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Potential insecticidal activity of aminonaphthoquinone Mannich bases derived from lawsone and their copper (II) complex derivatives.","authors":"Andréia P Matos,&nbsp;André L F Sarria,&nbsp;Ana C Volante,&nbsp;Antônio R Bernardo,&nbsp;Gracielle O S Cunha,&nbsp;João B Fernandes,&nbsp;Paulo C Vieira,&nbsp;Maria Fátima das G F da Silva","doi":"10.1515/znc-2020-0115","DOIUrl":"https://doi.org/10.1515/znc-2020-0115","url":null,"abstract":"<p><p>The fall armyworm, <i>Spodoptera frugiperda</i>, is a polyphagous pest that causes important damage in different regions of America and mainly affects corn crops in both tropical and subtropical areas. Currently, control relies on both transgenic plants and/or chemical pesticides. In this work, we describe insecticidal activity against the fall armyworm from a series of Mannich bases (<b>1-10</b>), derived from 2-hydroxy-1,4-naphthoquinone (lawsone), substituted benzaldehydes, and two primary amines, and their Cu²⁺ complexes (<b>11-20</b>). The [Cu(L)₂] complexes were more effective in larval mortality compared to the free Mannich bases. Among the tested compounds, complex <b>11</b> showed the highest toxicity, with 70.00% larval mortality.</p>","PeriodicalId":520830,"journal":{"name":"Zeitschrift fur Naturforschung. C, Journal of biosciences","volume":" ","pages":"111-115"},"PeriodicalIF":2.0,"publicationDate":"2020-10-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1515/znc-2020-0115","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38525087","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Distillation time effecting on the composition of <i>Origanum floribundum</i> essential oils and their antioxidant and antimicrobial activities.","authors":"Lamia Kerbouche,&nbsp;Mohamed Hazzit,&nbsp;Aoumeur Baaliouamer,&nbsp;Soraya Akretche","doi":"10.1515/znc-2020-0102","DOIUrl":"https://doi.org/10.1515/znc-2020-0102","url":null,"abstract":"<p><p>The essential oils (EOs) of <i>Origanum floribundum</i> Munby, an aromatic and medicinal plant endemic in Algeria, were extracted by different hydrodistillation times (30 min, 1, 2 and 3 h) and analyzed by GC and GC-MS. The chromatographic analysis showed that thymol (32.7-45.0%), <i>p</i>-cymene (16.8-23.1%) and <i>γ</i>-terpinene (21.6-28.7%) were the most prominent components of the oils. The antioxidant ability was measured using the reductive potential, thiobarbituric acid reactive substances (TBARS) assay and the inhibition of free radicals DPPH^● and ABTS^●+. Antibacterial activity was assessed by the disc diffusion method against three bacteria (<i>Escherichia coli</i>, <i>Staphylococcus aureus</i> and <i>Bacillus subtilis</i>) and one fungus (<i>Candida albicans</i>). Minimal inhibitory concentrations (MICs) were determined using a microdilution method. Thymol is one of the compounds of EOs, which are widely reported as very biologically active. Although the oil isolated for 30 min was the less-thymol rich, it was the most active with all the antioxidant tests. In the most cases, the antimicrobial activity showed the best results with oils isolated for 30 min and 3 h (MIC = 0.25-1.75 μL/mL). These results suggest that it might be possible to isolate the EO from this plant for a minimum distillation time to obtain oil that can give maximum biological activities.</p>","PeriodicalId":520830,"journal":{"name":"Zeitschrift fur Naturforschung. C, Journal of biosciences","volume":" ","pages":"153-160"},"PeriodicalIF":2.0,"publicationDate":"2020-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1515/znc-2020-0102","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38442586","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Design and synthesis of novel <i>n</i>-butyphthalide derivatives as promising botanical fungicides.","authors":"Zhongfu Luo,&nbsp;Yu Deng,&nbsp;Bilan Luo,&nbsp;Yong Li,&nbsp;Qing Lan,&nbsp;Judi Fan,&nbsp;Wei Xue,&nbsp;Lei Tang,&nbsp;Lingling Fan","doi":"10.1515/znc-2020-0192","DOIUrl":"https://doi.org/10.1515/znc-2020-0192","url":null,"abstract":"<p><p>In order to obtain novel botanical fungicides, three series of novel 6-substituted <i>n</i>-butyphthalide derivatives have been designed and synthesized <i>via</i> nucleophilic addition, reduction, nitrification, amination, sulfonation, Sandmeyer and Suzuki reaction. The mycelium growth rate method was used to evaluate the inhibition activity against eight phytopathogenic fungi <i>in vitro</i>. Preliminary bioassay tests showed that compounds <b>6f</b>, <b>6n</b>, <b>6p</b>, <b>6r</b> and <b>7a</b> exhibited better activity for some fungi at 50 μg/mL than the positive drug hymexazol and lead compound <i>n</i>-butyphthalide (NBP). The preliminary structure-activity relationships indicated that the antifungal activity is significantly affected by the substituents on the benzene ring.</p>","PeriodicalId":520830,"journal":{"name":"Zeitschrift fur Naturforschung. C, Journal of biosciences","volume":" ","pages":"117-127"},"PeriodicalIF":2.0,"publicationDate":"2020-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1515/znc-2020-0192","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38418375","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Inhibition of chikusetsusaponin IVa on inflammatory responses in RAW264.7 cell line via MAPK pathway.","authors":"Guangren Xu,&nbsp;Hongyu Lei,&nbsp;Qiaoling Yuan,&nbsp;Huiyu Chen,&nbsp;Jianming Su","doi":"10.1515/znc-2019-0107","DOIUrl":"https://doi.org/10.1515/znc-2019-0107","url":null,"abstract":"<p><p>Chikusetsusaponin IVa (CHS-IVa), a saponin from herb <i>Panacis japonicas</i>, possesses extensive biological activities. However, the roles and underlying mechanisms of CHS-IVa on inflammation have not been fully clarified in the setting of murine macrophages. In this study, we found that CHS-IVa effectively reduced the expression of inflammatory mediators, including interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α), interleukin-10 (IL-10), interleukin-1β (IL-1β), cyclooxygenase (COX-2), inducible nitric oxide synthase (iNOS) in lipopolysaccharide (LPS)-stimulated murine macrophage-like RAW264.7 cells. Meanwhile, CHS-IVa could also evidently bate the contents of nitric oxide (NO) and prostaglandin E2 (PGE2) in cell culture supernatants. Furthermore, the anti-inflammatory activity of CHS-IVa may be via diminishing the phosphorylation of extracellular regulated protein kinases (ERK), p38, and c-Jun N-terminal kinase (JNK). Collectively, these findings will help to understand of the anti-inflammatory effects and mechanisms of <i>P. japonicas</i> deeply, and suggest a validated therapeutic use as an anti-inflammatory medication.</p>","PeriodicalId":520830,"journal":{"name":"Zeitschrift fur Naturforschung. C, Journal of biosciences","volume":" ","pages":"103-110"},"PeriodicalIF":2.0,"publicationDate":"2020-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1515/znc-2019-0107","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38429313","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Cotinus coggygria</i> Scop. induces cell cycle arrest, apoptosis, genotoxic effects, thermodynamic and epigenetic events in MCF7 breast cancer cells.","authors":"Zlatina I Gospodinova,&nbsp;Istvan Zupkó,&nbsp;Noémi Bózsity,&nbsp;Vasilissa I Manova,&nbsp;Mariyana S Georgieva,&nbsp;Svetla J Todinova,&nbsp;Stefka G Taneva,&nbsp;Imre Ocsovszki,&nbsp;Maria E Krasteva","doi":"10.1515/znc-2020-0087","DOIUrl":"https://doi.org/10.1515/znc-2020-0087","url":null,"abstract":"<p><p>Current plant-derived anticancer therapeutics aim to reach higher effectiveness, to potentiate chemosensitivity and minimize the toxic side effects compared to conventional chemotherapy. <i>Cotinus coggygria</i> Scop. is a herb with high pharmacological potential, widely applied in traditional phytotherapy. Our previous study revealed that leaf aqueous ethanolic extract from <i>C. coggygria</i> exerts <i>in vitro</i> anticancer activity on human breast, ovarian and cervical cancer cell lines. The objective of the present research was to investigate possible molecular mechanisms and targets of the antitumor activity of the extract in breast cancer MCF7 cells through analysis of cell cycle and apoptosis, clonogenic ability assessment, evaluation of the extract genotoxic capacity, characterization of cells thermodynamic properties, and analysis on the expression of genes involved in cellular epigenetic processes. The obtained results indicated that in MCF7 cells <i>C. coggygria</i> extract causes S phase cell cycle arrest and triggers apoptosis, reduces colony formation, induces DNA damage, affects cellular thermodynamic parameters, and tends to inhibit the relative expression of <i>DNMT1</i>, <i>DNMT3a</i>, <i>MBD3</i>, and <i>p300</i>. Further studies on the targeted molecules and the extract anti-breast cancer potential on animal experimental model system, need to be performed in the future.</p>","PeriodicalId":520830,"journal":{"name":"Zeitschrift fur Naturforschung. C, Journal of biosciences","volume":" ","pages":"129-140"},"PeriodicalIF":2.0,"publicationDate":"2020-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1515/znc-2020-0087","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38418374","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anticancer effects of novel NSAIDs derivatives on cultured human glioblastoma cells.","authors":"Özlem Özdemir,&nbsp;Lisa Marinelli,&nbsp;Ivana Cacciatore,&nbsp;Michele Ciulla,&nbsp;Bugrahan Emsen,&nbsp;Antonio Di Stefano,&nbsp;Adil Mardinoglu,&nbsp;Hasan Turkez","doi":"10.1515/znc-2020-0093","DOIUrl":"https://doi.org/10.1515/znc-2020-0093","url":null,"abstract":"<p><p>Several epidemiologic, clinical and experimental reports indicate that nonsteroidal anti-inflammatory drugs (NSAIDs) could have a potential as anticancer agents. The aim of this study was the evaluation of cytotoxic potential in human glioblastoma cells of novel synthesized NSAID derivatives, obtained by linking, through a spacer, <i>α</i>-lipoic acid (ALA) to anti-inflammatory drugs, such as naproxen (AL-3, 11 and 17), flurbiprofen (AL-6, 13 and 19) and ibuprofen (AL-9, 15 and 21). The effects on the level of gene expression were also determined using quantitative real-time polymerase chain reaction (qRT-PCR) analysis. According to our results, NSAID derivatives exhibited concentration dependent cytotoxic effects on U87-MG cell line when compared with the control group. Moreover, treatment of the most active compounds (AL-3, AL-6 and AL-9) caused upregulation of tumor suppressor gene <i>PTEN</i> and downregulation of some oncogenes such as <i>AKT1, RAF1</i> and <i>EGFR</i>. In conclusion, our results revealed that AL-3, AL-6 and AL-9 could be suitable candidates for further investigation to develop new pharmacological strategies for the prevention of cancer.</p>","PeriodicalId":520830,"journal":{"name":"Zeitschrift fur Naturforschung. C, Journal of biosciences","volume":" ","pages":"329-335"},"PeriodicalIF":2.0,"publicationDate":"2020-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1515/znc-2020-0093","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40543985","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Erratum to: Transcriptome profiling reveals an IAA-regulated response to adventitious root formation in lotus seedling.","authors":"Cheng Libao,&nbsp;Jiang Runzhi,&nbsp;Yang Jianjun,&nbsp;Xu Xiaoyong,&nbsp;Zeng Haitao,&nbsp;Li Shuyan","doi":"10.1515/znc-2018-0133","DOIUrl":"https://doi.org/10.1515/znc-2018-0133","url":null,"abstract":"","PeriodicalId":520830,"journal":{"name":"Zeitschrift fur Naturforschung. C, Journal of biosciences","volume":" ","pages":"409"},"PeriodicalIF":2.0,"publicationDate":"2018-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1515/znc-2018-0133","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36483647","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Erratum to: A comparative proteomic analysis for adventitious root formation in lotus root (Nelumbo nucifera Gaertn).","authors":"Cheng Libao,&nbsp;Jiang Runzhi,&nbsp;Yang Mengli,&nbsp;Li Liangjun,&nbsp;Li Shuyan","doi":"10.1515/znc-2018-0134","DOIUrl":"https://doi.org/10.1515/znc-2018-0134","url":null,"abstract":"","PeriodicalId":520830,"journal":{"name":"Zeitschrift fur Naturforschung. C, Journal of biosciences","volume":" ","pages":"411"},"PeriodicalIF":2.0,"publicationDate":"2018-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1515/znc-2018-0134","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36483646","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Environmental alterations in biofuel generating molecules in Zilla spinosa.","authors":"Hemmat Khattab,&nbsp;Zeinab El Marid","doi":"10.1515/znc-2016-0151","DOIUrl":"https://doi.org/10.1515/znc-2016-0151","url":null,"abstract":"<p><p>Now days, production of fuels and petrochemicals from renewable lignocellulosic biomass is an indispensable issue to meet the growing energy demand. Meanwhile, the changes in the climate and soil topography influence the growth and development as well as canopy level of the lignocellulosic biomass. In this study, Zilla spinosa Turr (Zilla) plants with similar age and size were collected from three main sectors (upstream, midstream, and downstream) of Wadi Hagul during spring (April) and summer (July) seasons. Environmental stresses evoked reduction in the energy trapping pigments concomitant with increments in chlorophyll fluorescence in summer harvested plants particularly at downstream. Furthermore, the biofuels generating compounds including carbohydrate, lignin, and lipid making the plant biomasses are greatly affected by environmental conditions. Greater amount of lignin was estimated in summer harvested Z. spinosa shoots particularly at downstream. Moreover, the total oil content which is a promising source of biodiesel was considerably decreased during summer season particularly at downstream. The physical properties of the lipids major constituent fatty acid methyl esters determine the biofuel properties and contribute in the adaptation of plants against environmental stresses. Hence, the analysis of fatty acid profile showed significant modifications under combined drought and heat stress displayed in the summer season. The maximum increase in saturated fatty acid levels including tridecanoic acid (C13:0), pentadeanoic acid (C15:0), palmitic acid (C16:0), and stearic acid (C18:0) were estimated in spring harvested Z. spinosa aerial portions particularly at midstream. In spite of the reduction in the total oil content, a marked increase in the value of unsaturated to saturated fatty acids ratio and thereby the unsaturation index were achieved during the dry summer period. Henceforth, these seasonal and spatial variations in fatty acids profiles may contribute in the acclimatization of Z. spinosa plants to soil water scarcity associated with heat stress experienced during summer. In addition, the alterations in the fatty acid profiles may match biofuel requirements. In conclusion, the most adequate growing season (spring) will be decisive for achieving high lipid productivity associated with improved biofuel quality in terms of high saturated fatty acids percentage that improves its cetane number. However, the dry summer season enhanced the accumulation of greater amount of lignin that may enhance the biodiesel quantity.</p>","PeriodicalId":520830,"journal":{"name":"Zeitschrift fur Naturforschung. C, Journal of biosciences","volume":" ","pages":"77-91"},"PeriodicalIF":2.0,"publicationDate":"2017-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1515/znc-2016-0151","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39976276","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}