Reports of biochemistry & molecular biology最新文献_第2页

Frequency of bla _CTX-M and bla _TEM Virulence Genes and Antibiotic Resistance Profiles among Klebsiella pneumoniae Isolates in Urinary Tract Infection (UTI) Samples from Hashtgerd, Iran. 伊朗哈什格德地区尿路感染(UTI)肺炎克雷伯菌bla CTX-M和bla TEM毒力基因频率及耐药性分析

IF 1.7

Reports of biochemistry & molecular biology Pub Date : 2021-10-01 DOI: 10.52547/rbmb.10.3.412

Shirin Sarshar, Reza Mirnejad, Ebrahim Babapour

{"title":"Frequency of bla CTX-M and bla TEM Virulence Genes and Antibiotic Resistance Profiles among Klebsiella pneumoniae Isolates in Urinary Tract Infection (UTI) Samples from Hashtgerd, Iran.","authors":"Shirin Sarshar, Reza Mirnejad, Ebrahim Babapour","doi":"10.52547/rbmb.10.3.412","DOIUrl":"https://doi.org/10.52547/rbmb.10.3.412","url":null,"abstract":"Background: Klebsiella pneumoniae (K. pneumoniae) is an opportunistic microorganism and one of the most important causes of urinary tract infection. This study aimed to evaluate the frequency of K. pneumoniae producing broad-spectrum beta-lactamase in urinary tract infection and to determine the pattern of drug resistance.Methods: This study was performed on 50 samples of K. pneumoniae isolated from patients with urinary tract infection referred to the Medical Diagnostic Laboratory in Hashtgerd city. The isolates were first evaluated for antibiotic susceptibility by disk diffusion method according to the method proposed by the Clinical and Laboratory Standards Institute (CLSI). Then phenotypic detection of ESBLS was carried out by the DDST method. The frequency of gene bla TEM and bla CTX-M was determined by PCR.Results: The highest resistance was observed to ampicillin (94%) and the highest sensitivity was observed to gentamicin (84%). 22 isolates (44%) were positive for ESBLs production. Of the 50 isolates studied, 34% had bla CTX-M and 28% had bla TEM and 11 (22%) had both genes simultaneously. Also, more than 77% of positive ESBLs isolates had the bla CTX-M gene and approximately 63.64% of positive ESBLs isolates had the bla TEM gene.Conclusion: Given the high prevalence of antibiotic-resistant and ESBL-producing isolates, early identification of these resistant isolates and their follow-up is essential to prevent further outbreaks. It is also important to use appropriate therapeutic strategies and proper and rational administration of antibiotics by physicians.","PeriodicalId":520763,"journal":{"name":"Reports of biochemistry & molecular biology","volume":" ","pages":"412-419"},"PeriodicalIF":1.7,"publicationDate":"2021-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8718776/pdf/rbmb-10-412.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39659865","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Investigation of Decitabine Effects on HDAC3 and HDAC7 mRNA Expression in NALM-6 and HL-60 Cancer Cell Lines. 地西他滨对NALM-6和HL-60癌细胞HDAC3和HDAC7 mRNA表达的影响

IF 1.7

Reports of biochemistry & molecular biology Pub Date : 2021-10-01 DOI: 10.52547/rbmb.10.3.420

Sina Dalvand, Amin Namdari, Farzad Sepahvand, Mohammad Hassan Meshkibaf, GholamReza Ahmadpour

{"title":"Investigation of Decitabine Effects on HDAC3 and HDAC7 mRNA Expression in NALM-6 and HL-60 Cancer Cell Lines.","authors":"Sina Dalvand, Amin Namdari, Farzad Sepahvand, Mohammad Hassan Meshkibaf, GholamReza Ahmadpour","doi":"10.52547/rbmb.10.3.420","DOIUrl":"https://doi.org/10.52547/rbmb.10.3.420","url":null,"abstract":"Background: Decitabine is a potent anticancer hypomethylating agent and changes the gene expression through the gene's promoter demethylation and also independently from DNA demethylation. So, the present study was designed to distinguish whether Decitabine, in addition to inhibitory effects on DNA methyltransferase, can change HDAC3 and HDAC7 mRNA expression in NALM-6 and HL-60 cancer cell lines.Methods: HL-60, NALM-6, and normal cells were cultured, and the Decitabine treatment dose was obtained (1 µM) through the MTT assay. Finally, HDAC3 and HDAC7 mRNA expression were measured by Real-Time PCR in HL-60 and NALM-6 cancerous cells before and after treatment. Furthermore, HDAC3 and HDAC7 mRNA expression in untreated HL-60 and NALM-6 cancerous cells were compared to normal cells.Results: Our results revealed that the expression of HDAC3 and HDAC7 in HL-60 and NALM-6 cells increases as compared to normal cells. After treatment of HL-60 and NALM-6 cells with Decitabine, HDAC3, and HDAC7 mRNA expression were decreased significantly.Conclusion: Our data confirmed that the effects of Decitabine are not limited to direct hypomethylation of DNMTs, but it can indirectly affect other epigenetic factors, such as HDACs activity, through converging pathways.","PeriodicalId":520763,"journal":{"name":"Reports of biochemistry & molecular biology","volume":" ","pages":"420-428"},"PeriodicalIF":1.7,"publicationDate":"2021-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8718785/pdf/rbmb-10-420.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39659866","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Impact of Berberine on Some Epigenetic, Transcription Regulation and Inflammatory Biomarkers in a Mice Model of Epilepsy. 小檗碱对癫痫小鼠模型中一些表观遗传、转录调控和炎症生物标志物的影响。

IF 1.7

Reports of biochemistry & molecular biology Pub Date : 2021-10-01 DOI: 10.52547/rbmb.10.3.362

Heba Bassiony Ghanem, Marwa Nagy Emam, Ali Dareen Abdelaziz Mohammed, Abd-Ellatif Rania Nagi

{"title":"Impact of Berberine on Some Epigenetic, Transcription Regulation and Inflammatory Biomarkers in a Mice Model of Epilepsy.","authors":"Heba Bassiony Ghanem, Marwa Nagy Emam, Ali Dareen Abdelaziz Mohammed, Abd-Ellatif Rania Nagi","doi":"10.52547/rbmb.10.3.362","DOIUrl":"https://doi.org/10.52547/rbmb.10.3.362","url":null,"abstract":"Background: Epilepsy is one of the most widespread neurological disease worldwide. Status epilepticus (SE) is a life-threatening neurologic disorder. Neuroprotective approaches are increasingly to discover a promising therapy to manage epileptic disorders. This study aimed to assess the impact of berberine on some epigenetic, transcription regulation & inflammatory biomarkers in a mice model of epilepsy.Methods: This work was performed on; Group I: (control), Group II: berberine-treated control,Group III: epilepsy group, Group IV: berberine-treated epilepsy. Groups were subjected to assessment of Tumor growth factor-1β (TGF-1β), hypoxia inducible factor-1α (HIF-1α), brain derived neurotrophic factor (BDNF) levels, histone deacetylase (HDAC) activity & neuronal restrictive silencing factor (NRSF) gene expression.Results: Study showed significant increase in levels of HIF-1α, TGF-1β, HDAC activity & NRSF gene expression in epilepsy group & decrease in these levels in berberine treated epilepsy group. Significant decrease in BDNF levels in epilepsy & elevation in them in berberine treated epilepsy group.Conclusion: Our study showed the anti-epileptic impact of berberine via its regulatory effect on some epigenetic, transcription factors & inflammatory biomarkers in a mice model of epilepsy.","PeriodicalId":520763,"journal":{"name":"Reports of biochemistry & molecular biology","volume":" ","pages":"362-372"},"PeriodicalIF":1.7,"publicationDate":"2021-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8718772/pdf/rbmb-10-362.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39644298","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Ghrelin Is Effective on Passive Avoidance Memory by Altering the Expression of NMDAR and HTR1a Genes in the Hippocampus of Male Wistar Rats. Ghrelin通过改变雄性Wistar大鼠海马NMDAR和HTR1a基因的表达对被动回避记忆有影响。

IF 1.7

Reports of biochemistry & molecular biology Pub Date : 2021-10-01 DOI: 10.52547/rbmb.10.3.380

Vahideh Sahraiian, Homayoun Khazali

{"title":"Ghrelin Is Effective on Passive Avoidance Memory by Altering the Expression of NMDAR and HTR1a Genes in the Hippocampus of Male Wistar Rats.","authors":"Vahideh Sahraiian, Homayoun Khazali","doi":"10.52547/rbmb.10.3.380","DOIUrl":"https://doi.org/10.52547/rbmb.10.3.380","url":null,"abstract":"Background: Memory-dependent psychological behaviors have an important role in life. Memory strengthening in adulthood to prevent its defects in aging is a significant issue. The ghrelin endogenous hormone improves memory by targeting glutamatergic and serotonergic circuits. Also, citicoline, a memory strengthening drug in aging, is not recommended to adults due to its side effects. The current study aims to test that ghrelin treatment, like citicoline, would improve passive avoidance memory via expression of the genes encoding the N-methyl-D-aspartate receptor (NMDAR1) and the serotonin receptor 1A (HTR1α) involved in this process.Methods: Five groups of adult male rats received (1) saline (as control), (2) 0.5 mg/kg citicoline, or (3-5) 0.3, 1.5, and 3 nmol/μl ghrelin). The rats received the drugs via intra-hippocampal injection. Passive avoidance memory was determined using a shuttle box device. The latency to enter the dark chamber before (IL) and after (RL) injection and the total duration of the animal's presence in the light compartment (TLC) were evaluated. Then, the gene expression rates of NMDAR1 and HTR1α were measured by the Real-Time PCR.Results: Ghrelin and citicoline had some similar and significant effects on passive avoidance memory, and both increased NMDAR1 and decreased HTR1α expression.Conclusion: Ghrelin, like citicoline, improves passive avoidance learning by altering the NMDAR1 and HTR1α expression in the hippocampus.","PeriodicalId":520763,"journal":{"name":"Reports of biochemistry & molecular biology","volume":" ","pages":"380-386"},"PeriodicalIF":1.7,"publicationDate":"2021-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8718781/pdf/rbmb-10-380.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39659861","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

The Cytokines Responses against Parvovirus B19 in Miscarriage Women and the Susceptibility of their RhD Blood Type to Contract Parvovirus B19 in South of Iraq. 伊拉克南部流产妇女细胞因子对细小病毒B19的应答及RhD血型对细小病毒B19的易感性

IF 1.7

Reports of biochemistry & molecular biology Pub Date : 2021-10-01 DOI: 10.52547/rbmb.10.3.462

Dhurgham Al-Fahad, Khalid Majeed, Ahmed Al-Naqshbandi, Ahmed Al-Amery, Bandar Fahad Alharbi

{"title":"The Cytokines Responses against Parvovirus B19 in Miscarriage Women and the Susceptibility of their RhD Blood Type to Contract Parvovirus B19 in South of Iraq.","authors":"Dhurgham Al-Fahad, Khalid Majeed, Ahmed Al-Naqshbandi, Ahmed Al-Amery, Bandar Fahad Alharbi","doi":"10.52547/rbmb.10.3.462","DOIUrl":"https://doi.org/10.52547/rbmb.10.3.462","url":null,"abstract":"Background: Parvovirus B19 (B19) infection is linked with various diseases. Cytokines play critical roles in cellular response to viral infection. It has also been reported that's susceptibility of the ABO blood type people to several viral infection. In this study, we evaluated interleukin 6 (IL-6), interleukin 8(IL-8), and interferon gamma (IFN-γ) levels in aborted women infected with parvovirus B19 (B19+/Abr+) and uninfected with B19(B19-/Abr+) in comparison with healthy women (B12-/Abr-) and susceptibility of their RhD blood type to contract B19.Methods: B19+/Abr+ were diagnosed using IgM and IgG antibodies against B19, and the concentrations of IL-6, IL-8, and IFN-γ were determined using enzyme-linked immunosorbent assay (ELISA) test in both B19+/Abr+, B19-/Abr+, and B19-/Abr-. Here, we also collected blood groups, number of abortion, and gestational ages from 200 B19+/Abr+ along with the same number ofB19-/Abr+ and B19-/Abr-.Results: The levels of IFN-γ were higher in serum of B19-/Abr+andB19+/Abr+ group in comparison to B19-/Abr-, while the serum levels of IL-6, IL-8were increased in B19+/Abr+ group in comparisontoB19-/Abr+ and B19-/Abr-. Our analyzed data also showed that aborted women with RhD+ are more susceptible to contract s B19 than people with RhD- blood type.Conclusion: B19 infection may differently modulate the amount of cytokines in the plasma of aborted women. So, it can be suggested that IL-6, IL-8, and IFN-γ potentially useful as markers for inflammation intrauterine. The susceptibility/protection of aborted women against B19 might be determined based on RhD blood type.","PeriodicalId":520763,"journal":{"name":"Reports of biochemistry & molecular biology","volume":" ","pages":"462-470"},"PeriodicalIF":1.7,"publicationDate":"2021-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8718769/pdf/rbmb-10-462.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39895962","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Analysis of Micro-RNA-144 Expression Profile in Patients with Multiple Sclerosis in Comparison with Healthy Individuals. 多发性硬化症患者与健康人Micro-RNA-144表达谱的比较分析

IF 1.7

Reports of biochemistry & molecular biology Pub Date : 2021-10-01 DOI: 10.52547/rbmb.10.3.396

Fatemeh Roshani, Hossien Delavar Kasmaee, Kowsar Falahati, Ghazaleh Arabzade, Atefeh Sohan Forooshan Moghadam, Mohammad Hossein Sanati

{"title":"Analysis of Micro-RNA-144 Expression Profile in Patients with Multiple Sclerosis in Comparison with Healthy Individuals.","authors":"Fatemeh Roshani, Hossien Delavar Kasmaee, Kowsar Falahati, Ghazaleh Arabzade, Atefeh Sohan Forooshan Moghadam, Mohammad Hossein Sanati","doi":"10.52547/rbmb.10.3.396","DOIUrl":"https://doi.org/10.52547/rbmb.10.3.396","url":null,"abstract":"Background\u0000Etiology of multiple sclerosis is non-clarified. It seems that environmental factors impact epigenetic in this disease. Micro-RNAs (MIR) as epigenetic factors are one of the most important factors in non-genetically neurodegenerative diseases. It has been found MIR-144 plays a main role in the regulation of many processes in the central nervous system. Here, we aimed to investigation of MIR-144 expression alteration in Multiple sclerosis (MS) patients.\u0000\u0000\u0000Methods\u0000In this study 32 healthy and 32 MS patient's blood sample were analyzed by quantitative Real-Time PCR method and obtained data analyzed by REST 2009 software.\u0000\u0000\u0000Results\u0000Analysis of Real-Time PCR data revealed that miR-144 Increase significantly in MS patients compared to healthy controls.\u0000\u0000\u0000Conclusion\u0000The increase of MIR-144 expression in MS patients is obvious. MIR-144 can be used as a biomarker of MS and help to early diagnosis and treatment of this disease.","PeriodicalId":520763,"journal":{"name":"Reports of biochemistry & molecular biology","volume":" ","pages":"396-401"},"PeriodicalIF":1.7,"publicationDate":"2021-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8718777/pdf/rbmb-10-396.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39659863","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

The Effects of Pre-Treatment and Post-Treatment of Thymol against tert-Butyl Hydroperoxide (t-BHP) Cytotoxicity in MCF-7 Cell Line and Fibroblast Derived Foreskin. 百里香酚预处理和后处理对MCF-7细胞系和成纤维细胞包皮叔丁基过氧化氢(t-BHP)细胞毒性的影响

IF 1.7

Reports of biochemistry & molecular biology Pub Date : 2020-10-01 DOI: 10.29252/rbmb.9.3.338

Afsaneh Dashtaki, Soleiman Mahjoub, Ebrahim Zabihi, Roghayeh Pourbagher

{"title":"The Effects of Pre-Treatment and Post-Treatment of Thymol against tert-Butyl Hydroperoxide (t-BHP) Cytotoxicity in MCF-7 Cell Line and Fibroblast Derived Foreskin.","authors":"Afsaneh Dashtaki, Soleiman Mahjoub, Ebrahim Zabihi, Roghayeh Pourbagher","doi":"10.29252/rbmb.9.3.338","DOIUrl":"https://doi.org/10.29252/rbmb.9.3.338","url":null,"abstract":"Background: Some recent studies have reported anti-tumor activity for Thymol, but the findings are inconsistent. This study aimed to investigate and compare Thymol's effects on MCF-7 cancer cells and fibroblasts while treated with tert-Butyl hydroperoxide (t-BHP).Methods: In the pre-treatment, MCF-7 and fibroblast cells were treated with various Thymol concentrations and incubated for 24 h. Then, t-BHP was added to a final concentration of 50 μM, and the cells were incubated for one h. In the post-treatment, cells were incubated first with 50 μM t-BHP for one h and then treated with Thymol. Cell viability was tested by 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Thymol's antioxidant capacity was measured by DPPH and FRAP assays, and lipid peroxidation levels were determined by the TBARS method.Results: The thymol effects were dose-dependent, and despite their antioxidant properties, at concentrations of 100 µg/ml or more, increased t-BHP toxicity and reduced cancer cell viability. MTT assay result showed that pre-treatment and post-treatment with Thymol for 24 hours effectively reduced MCF-7 and fibroblast cell viability compared with the untreated control group. Both pre- and post-treatment of Thymol, normal fibroblast cell viability was significantly greater than that of the MCF-7 cells.Conclusion: Our finding showed that Thymol appears to be toxic to MCF-7 cells at lower concentrations than fibroblasts after 24 hours of incubation. Pre-treatment with Thymol neutralized the oxidative effect of t-BHP in fibroblasts but was toxic for MCF-7 cells.","PeriodicalId":520763,"journal":{"name":"Reports of biochemistry & molecular biology","volume":" ","pages":"338-347"},"PeriodicalIF":1.7,"publicationDate":"2020-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7816789/pdf/rbmb-9-338.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25419243","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Association of Glutathione S-Transferase theta 1 and mu 1 Genes Polymorphisms with the Susceptibility of Myocardial Infarction in Bangladesh. 谷胱甘肽s -转移酶theta 1和mu 1基因多态性与孟加拉国心肌梗死易感性的关系

IF 1.7

Reports of biochemistry & molecular biology Pub Date : 2020-10-01 DOI: 10.29252/rbmb.9.3.366

Jyosna Khanam, Delowar Hossain, Bayejid Hosen, Mesbah Uddin, Asadul Kabir, Mohammad Anwarul Bari

{"title":"Association of Glutathione S-Transferase theta 1 and mu 1 Genes Polymorphisms with the Susceptibility of Myocardial Infarction in Bangladesh.","authors":"Jyosna Khanam, Delowar Hossain, Bayejid Hosen, Mesbah Uddin, Asadul Kabir, Mohammad Anwarul Bari","doi":"10.29252/rbmb.9.3.366","DOIUrl":"https://doi.org/10.29252/rbmb.9.3.366","url":null,"abstract":"Background: Myocardial infarction is one of the leading causes of morbidity and mortality worldwide. Oxidative stress plays a vital role in the pathogenesis of atherosclerosis leading to myocardial infarction and Glutathione S-transferases (GSTs) act as detoxifying enzymes to reduce oxidative stress. The aim of the present study was to investigate the associations of the GST (T1 & M1) gene polymorphism with the susceptibility of myocardial infarction in the Bangladeshi population.Methods: A case-control study on 100 cardiac patients with MI and 150 control subjects was conducted. The genotyping of GST (T1 & M1) gene was done using conventional Polymerase Chain Reaction.Results: The percentage of GSTM1 genotypes was significantly (p< 0.01) lower in patients compared to control subjects while the GSTT1 genotypes were not significantly different between the study subjects. The individual with GSTM1 null allele was at 2.5-fold increased risk {odds ratio (OR)= 2.5; 95 % confidence interval (95 % CI)= 1.4 to 4.3; p< 0.01} of experiencing MI while individual with either GSTM1 or GSTT1 genotypes was at lower risk. In the case of GST M1 and GST T1 combined genotype, patients having both null genotypes for GST M1 and GST T1 gene showed significantly (p< 0.01) higher risk of experiencing MI when compared to control subjects (OR= 3.5; 95% CI= 1.7-7.2; p< 0.001).Conclusion: Thus our recent study suggested that GSTM1 alone and GSTM1 and T1 in combination augments the risk of MI in Bangladeshi population.","PeriodicalId":520763,"journal":{"name":"Reports of biochemistry & molecular biology","volume":" ","pages":"366-372"},"PeriodicalIF":1.7,"publicationDate":"2020-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7816778/pdf/rbmb-9-366.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25419246","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Noninvasive Fetal Sex Determination by Real-Time PCR and TaqMan Probes. 实时荧光定量PCR和TaqMan探针的无创胎儿性别测定。

IF 1.7

Reports of biochemistry & molecular biology Pub Date : 2020-10-01 DOI: 10.29252/rbmb.9.3.315

Mohammad Hossein Ahmadi, Naser Amirizadeh, Maryam Rabiee, Fatemeh Rahimi-Sharbaf, Ali Akbar Pourfathollah

{"title":"Noninvasive Fetal Sex Determination by Real-Time PCR and TaqMan Probes.","authors":"Mohammad Hossein Ahmadi, Naser Amirizadeh, Maryam Rabiee, Fatemeh Rahimi-Sharbaf, Ali Akbar Pourfathollah","doi":"10.29252/rbmb.9.3.315","DOIUrl":"https://doi.org/10.29252/rbmb.9.3.315","url":null,"abstract":"Background: Noninvasive fetal sex determination by analyzing Y chromosome-specific sequences is very useful in the management of cases related to sex-linked genetic diseases. The aim of this study was to establish a non-invasive fetal sex determination test using Real-Time PCR and specific probes.Methods: The study was a prospective observational cohort study conducted from August 2018 to September 2019. Venous blood samples were collected from 25 Iranian pregnant women at weeks 7 to 25 of gestation. Cell-free DNA (cfDNA) was isolated from the plasma of samples and fetal sex was determined by SRY gene analysis using the Real-Time PCR technique. In the absence of SRY detection, the presence of fetal DNA was investigated using cfDNA treated with BstUI enzyme and PCR for the epigenetic marker RASSF1A.Results: Of the total samples analyzed, 48% were male and 52% female. The RASSF1A assay performed on SRY negative cases also confirmed the presence of cell-free fetal DNA. Genotype results were in full agreement with neonate gender, and the accuracy of noninvasive fetal sex determination was 100%.Conclusion: Fetal sex determination using the strategy applied in this study is noninvasive and highly accurate and can be exploited in the management of sex-linked genetic diseases.","PeriodicalId":520763,"journal":{"name":"Reports of biochemistry & molecular biology","volume":" ","pages":"315-323"},"PeriodicalIF":1.7,"publicationDate":"2020-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7816785/pdf/rbmb-9-315.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25418829","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Molecular Detection of Campylobacter Species: Comparision of 16SrRNA with slyD, cadF, rpoA, and dnaJ Sequencing. 弯曲杆菌种类的分子检测:16SrRNA与sld、cadF、rpoA和dnaJ测序的比较。

IF 1.7

Reports of biochemistry & molecular biology Pub Date : 2020-10-01 DOI: 10.29252/rbmb.9.3.257

Elahe Ghorbani Marghmaleki, Azam Ahmadi, Mohammad Arjomandzadegan, Majid Akbari, Aysan Karamghoshchi

{"title":"Molecular Detection of Campylobacter Species: Comparision of 16SrRNA with slyD, cadF, rpoA, and dnaJ Sequencing.","authors":"Elahe Ghorbani Marghmaleki, Azam Ahmadi, Mohammad Arjomandzadegan, Majid Akbari, Aysan Karamghoshchi","doi":"10.29252/rbmb.9.3.257","DOIUrl":"https://doi.org/10.29252/rbmb.9.3.257","url":null,"abstract":"Background: Campylobacter spp. are the main cause of human gastroenteritis. The 16SrRNA sequencing is one of fast molecualr method to detect this fastidious. In this study, we compared the sequencing of 16srRNA genewith four housekeeping genestodetect Campylobacter spp. in patients with diarrhea and healthy people.Methods: 60 samples of Campylobacter DNA extracted from stool samples of 30 patients with diarrhea and 30 healthy people were used. In order to detect Campylobacter, we designed primers for proliferation of 16SrRNA, cadF, dnaJ, slyD , and rpoA genes using Primer 3, Mega 4.0 and Blast software. Then the PCR products were sequenced using ABI system.Results: The sequencing showed concordance of PCR-products with deposited sequences in the Gene Bank. Among diarrhea patients, 53.3% of samples were significantly (p< 0.05) positive for slyD and cadF genes and 50% of samples were positive using 16SrRNA, rpoA, and dnaJ genes by PCR assay. The average of sensitivity and specificity were found 53.33% and 83.33%, respectively.Conclusion: Due to various copies of repeated sequences of 16SrRNA gene, analyzing its amplicons on electrophoresis may be more difficult than the slyD and cadF genes. According to our results, among the 5 studied genes; the highest detection rate was related to slyD and cadF genes. Although, dnaJ and rpoA genes,instead of 16SrRNA gene, can be considered as appropriate genes for molecular detection of Campylobacter bacteria.","PeriodicalId":520763,"journal":{"name":"Reports of biochemistry & molecular biology","volume":" ","pages":"257-263"},"PeriodicalIF":1.7,"publicationDate":"2020-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7816790/pdf/rbmb-9-257.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25426867","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1