Reports of biochemistry & molecular biology最新文献_第3页

H19/Igf2 Expression and Methylation of Histone 3 in Mice Chimeric Blastocysts. 小鼠嵌合囊胚中H19/Igf2的表达和组蛋白3的甲基化

IF 1.7

Reports of biochemistry & molecular biology Pub Date : 2020-10-01 DOI: 10.29252/rbmb.9.3.357

Maryam Salimi, Abolfazl Shirazi, Mohsen Norouzian, Ameneh Jafari, Haleh Edalatkhah, Maryam Mehravar, Mohammad Majidi, Mohammad Mahdi Mehrazar

{"title":"H19/Igf2 Expression and Methylation of Histone 3 in Mice Chimeric Blastocysts.","authors":"Maryam Salimi, Abolfazl Shirazi, Mohsen Norouzian, Ameneh Jafari, Haleh Edalatkhah, Maryam Mehravar, Mohammad Majidi, Mohammad Mahdi Mehrazar","doi":"10.29252/rbmb.9.3.357","DOIUrl":"https://doi.org/10.29252/rbmb.9.3.357","url":null,"abstract":"Background: Currently, the efficient production of chimeric mice and their survival are still challenging. Recent researches have indicated that preimplantation embryo culture media and manipulation lead to abnormal methylation of histone in the H19/Igf2 promotor region and consequently alter their gene expression pattern. This investigation was designed to evaluate the relationship between the methylation state of histone H3 and H19/Igf2 expression in mice chimeric blastocysts.Methods: Mouse 129/Sv embryonic stem cells (mESCs) expressing the green fluorescent protein (mESCs-GFP) were injected into the perivitelline space of 2.5 days post-coitis (dpc) embryos (C57BL/6) using a micromanipulator. H3K4 and H3K9 methylation, and H19 and Igf2 expression was measured by immunocytochemistry and q-PCR, respectively, in blastocysts.Results: Histone H3 trimethylation in H3K4 and H3K9 in chimeric blastocysts was significantly less and greater, respectively (p< 0.05), than in controls. H19 expression was significantly less (p< 0.05), while Igf2 expression was less, but not significantly so, in chimeric than in control blastocysts.Conclusion: Our results showed, that the alteration ofH3K4me3 and H3K9me3 methylation, change H19/Igf2 expression in chimeric blastocysts.","PeriodicalId":520763,"journal":{"name":"Reports of biochemistry & molecular biology","volume":" ","pages":"357-365"},"PeriodicalIF":1.7,"publicationDate":"2020-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7816783/pdf/rbmb-9-357.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25419245","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparison of Osteogenic Potential of Phenytoin with Dexamethasone in Cultured Dental Pulp Stem Cells. 苯妥英与地塞米松在培养牙髓干细胞成骨潜能的比较。

IF 1.7

Reports of biochemistry & molecular biology Pub Date : 2020-10-01 DOI: 10.29252/rbmb.9.3.331

Mitra Asgharian-Rezaee, Raheleh Alipour-Farmad, Zahra Tayarani-Najaran

{"title":"Comparison of Osteogenic Potential of Phenytoin with Dexamethasone in Cultured Dental Pulp Stem Cells.","authors":"Mitra Asgharian-Rezaee, Raheleh Alipour-Farmad, Zahra Tayarani-Najaran","doi":"10.29252/rbmb.9.3.331","DOIUrl":"https://doi.org/10.29252/rbmb.9.3.331","url":null,"abstract":"Background: One of the adverse effects of phenytoin (diphenylhydantoin, DPH) is enlargement of facial features. Although there are some reports on anabolic action of phenytoin on bone cells, the osteogenic potential of DPH on mesenchymal stem cells has not been studied. The purpose of this study was to evaluate the osteogenic potential of DPH on dental pulp stem cells (DPSCs).Methods: Human DPSCs were isolated and characterized by flow cytometry; presence of CD29 and CD44 and absence of CD34 and CD45 were performed to confirm the mesenchymal stem cells. Isolated DPSCs were differentiated either in conventional osteogenic medium with Dexamethasone or medium containing different concentration of phenytoin (12.5, 25, 100, and 200 µM). The osteogenic differentiation evaluated by performing western blot test for Runt-related transcription factor 2 (RUNX2), osteopontin and alkaline phosphatase (ALP) also alizarin red S staining to measure the mineralization of cells.Results: Our results showed morphological changes and mineralization of DPSCs by using DPH were comparable with dexamethasone. Moreover, western blot results of DPH group showed significant increase of ALP, RUNX2 and osteopontin (OSP) in comparison with control.Conclusion: The data of present study showed the osteogenic activity of phenytoin, considering as an alternative of dexamethasone for inducing osteogenic differentiation of dental pulp stem cells.","PeriodicalId":520763,"journal":{"name":"Reports of biochemistry & molecular biology","volume":" ","pages":"331-337"},"PeriodicalIF":1.7,"publicationDate":"2020-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7816787/pdf/rbmb-9-331.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25418831","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

The Antimicrobial Peptide, Nisin, Synergistically Enhances the Cytotoxic and Apoptotic Effects of Rituximab Treatment on Human Burkitt's Lymphoma Cell Lines. 抗菌肽Nisin协同增强利妥昔单抗治疗对人伯基特淋巴瘤细胞系的细胞毒性和凋亡作用

IF 1.7

Reports of biochemistry & molecular biology Pub Date : 2020-10-01 DOI: 10.29252/rbmb.9.3.250

Pantea Mohammadi, Mina Zangeneh, Hamid-Reza Mohammadi-Motlagh, Fatemeh Khademi

{"title":"The Antimicrobial Peptide, Nisin, Synergistically Enhances the Cytotoxic and Apoptotic Effects of Rituximab Treatment on Human Burkitt's Lymphoma Cell Lines.","authors":"Pantea Mohammadi, Mina Zangeneh, Hamid-Reza Mohammadi-Motlagh, Fatemeh Khademi","doi":"10.29252/rbmb.9.3.250","DOIUrl":"https://doi.org/10.29252/rbmb.9.3.250","url":null,"abstract":"Background: Non-Hodgkin's lymphomas comprise the most common hematological cancers worldwide and consist of a heterogenous group of malignancies affecting the lymphoid system. Treatment of non-Hodgkin's lymphoma has been significantly enhanced with the addition of Rituximab to the standard chemotherapy regimen. However, even with the advancement of treatment patients continue to relapse and develop resistance to Rituximab, rendering subsequent treatments unsuccessful. The use of novel drugs with unique antitumor mechanisms has gained considerable attention. In this study, we explored the in vitro anti-cancer effects of the combined therapy of Rituximab and Nisin on human Burkitt's lymphoma cells.Methods: The human Burkitt's lymphoma cells lines, Raji and Daudi, were treated with Nisin, Rituximab, or a combination of the two agents at various concentrations. Cytotoxicity following treatment was determined using cell viability assay. The degree of apoptosis was verified via flow cytometric analysis using FITC annexin V/PI staining.Results: Our findings show that the combined treatment of Rituximab and Nisin results in a more significant reduction in the survival of Raji and Daudi Burkitt's lymphoma cells, compared to Nisin or Rituximab treatment alone. Additionally, our results indicate that Nisin can induce a significant degree of apoptosis in the Burkitt's lymphoma cells compared to the negative controls. However, the addition of Nisin to the Rituximab treatment synergistically enhances the apoptotic antitumor effect.Conclusion: This study demonstrates the synergistic antitumor effect of Nisin treatment in vitro to enhance tumor cell apoptosis and the potential value of Nisin as an adjunct therapy in the treatment of lymphoma.","PeriodicalId":520763,"journal":{"name":"Reports of biochemistry & molecular biology","volume":" ","pages":"250-256"},"PeriodicalIF":1.7,"publicationDate":"2020-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7816786/pdf/rbmb-9-250.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25426866","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Novel and Efficient Protocol for DNA Coating-Based Identification of DNA-Protein Interaction by Antibody-Mediated Immunodetection. 基于DNA涂层的抗体介导免疫检测鉴定DNA-蛋白相互作用的新方法。

IF 1.7

Reports of biochemistry & molecular biology Pub Date : 2020-10-01 DOI: 10.29252/rbmb.9.3.264

Chellathurai Vasantha Niranjan, Selvan Johnson Retnaraj Samue, Venkatachalam Saravanakumar, Selvan Jackson Durairaj

{"title":"Novel and Efficient Protocol for DNA Coating-Based Identification of DNA-Protein Interaction by Antibody-Mediated Immunodetection.","authors":"Chellathurai Vasantha Niranjan, Selvan Johnson Retnaraj Samue, Venkatachalam Saravanakumar, Selvan Jackson Durairaj","doi":"10.29252/rbmb.9.3.264","DOIUrl":"https://doi.org/10.29252/rbmb.9.3.264","url":null,"abstract":"Background: Studying protein-protein and protein-DNA interactions are prerequisites for the identification of function and mechanistic role of various proteins in the cell. Protocols for analyzing DNA-based Protein-Protein and Protein-DNA interactions are complicated and need to be simplified for efficient tracking of binding capabilities of various proteins to specific DNA molecules. Here, we demonstrated a simple yet efficient protocol for the identification of DNA coating-based Protein-DNA interaction using antibodymediated immunodetection.Methods: Briefly, we have coated specific DNA in the microtiter plate followed by incubating with protein lysate. Specific protein-DNA and/or protein-protein bind with DNA interactions are identified using specific fluorophore-conjugated antibodies. Antibodies are used to detect a protein that is bound to the DNA.Results: Fluorescent-based detection identifies the specific interaction between Protein-DNA with respect to coated DNA fragments. The protocol uses indirect conjugated antibodies and hence the technique is sensitive for effective identification of Protein-DNA interactions.Conclusion: Based on the results we conclude that the demonstrated protocol is simple, efficient and sensitive for identification of Protein-DNA interactions.","PeriodicalId":520763,"journal":{"name":"Reports of biochemistry & molecular biology","volume":" ","pages":"264-269"},"PeriodicalIF":1.7,"publicationDate":"2020-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7816784/pdf/rbmb-9-264.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25426868","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Recombinant Tandem Repeated Expression of S3 and SΔ3 Antimicrobial Peptides. S3和SΔ3抗菌肽的重组串联重复表达。

IF 1.7

Reports of biochemistry & molecular biology Pub Date : 2020-10-01 DOI: 10.29252/rbmb.9.3.348

Sadegh Rezaei, Shahin Hadadian, Ramazan Ali Khavari-Nejad, Dariush Norouzian

{"title":"Recombinant Tandem Repeated Expression of S3 and SΔ3 Antimicrobial Peptides.","authors":"Sadegh Rezaei, Shahin Hadadian, Ramazan Ali Khavari-Nejad, Dariush Norouzian","doi":"10.29252/rbmb.9.3.348","DOIUrl":"https://doi.org/10.29252/rbmb.9.3.348","url":null,"abstract":"Background: Antimicrobial peptides (AMPs) are promising candidates for new generations of antibiotics to overcome the threats of multidrug-resistant infections as well as other industrial applications. Recombinant expression of small peptides is challenging due to low expression rates and high sensitivity to proteases. However, recombinant multimeric or fusion expression of AMPs facilitates cost-effective large-scale production of AMPs. In This project, S3 and SΔ3 AMPs were expressed as fusion partners. S3 peptide is a 34 amino acid linear antimicrobial peptide derived from lipopolysaccharide (LPS) binding site of factor C of horseshoe crab hemolymph and SΔ3 is a modified variant of S3 possessing more positive charges.Methods: Two copy tandem repeat of the fusion protein (named as SΔ3S3-2mer-GS using glycine- serine linker was expressed in E. coli. BL21 (DE3). After cell disruption and solubilization of inclusion bodies, the protein was purified by Ni -NTA affinity chromatography. Antimicrobial activity and cytotoxic properties of purified SΔ3S3-2mer-GS were compared with a previously produced tetramer of S3 with the same glycine- serine linker (S3-4mer-GS) and each of monomeric blocks of S3 and SΔ3.Results: SΔ3S3-2mer-GS was successfully expressed with an expression rate of 26%. The geometric average of minimum inhibitory concentration (MIC GM) of SΔ3S3-2mer-GS was 28%, 34%, and 57% lower than SΔ3, S3-4mer-GS, and S3, respectively. SΔ3S3-2mer-GS had no toxic effect on eukaryotes human embryonic kidney cells at its MIC concentration.Conclusion: tandem repeated fusion expression strategy could be employed as an effective technique for recombinant production of AMPs.","PeriodicalId":520763,"journal":{"name":"Reports of biochemistry & molecular biology","volume":" ","pages":"348-356"},"PeriodicalIF":1.7,"publicationDate":"2020-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7816777/pdf/rbmb-9-348.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25419244","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Protective Effects of Quercetin and Melatonin on Indomethacin Induced Gastric Ulcers in Rats. 槲皮素和褪黑素对吲哚美辛致大鼠胃溃疡的保护作用。

IF 1.7

Reports of biochemistry & molecular biology Pub Date : 2020-10-01 DOI: 10.29252/rbmb.9.3.278

Marwa Sayed Abdel-Tawab, Ola Mostafa Tork, Gomaa Mostafa-Hedeab, Manal Ewaiss Hassan, Dalia Azmy Elberry

{"title":"Protective Effects of Quercetin and Melatonin on Indomethacin Induced Gastric Ulcers in Rats.","authors":"Marwa Sayed Abdel-Tawab, Ola Mostafa Tork, Gomaa Mostafa-Hedeab, Manal Ewaiss Hassan, Dalia Azmy Elberry","doi":"10.29252/rbmb.9.3.278","DOIUrl":"https://doi.org/10.29252/rbmb.9.3.278","url":null,"abstract":"Background: Medications to prevent the development of NSAID-induced gastric ulcers have a large range of unpleasant side effects. Recent efforts have been focused on determining safer alternative nontoxic and natural forms of anti-ulcer treatments.Methods: Twenty-four male rats were divided into 4 groups: 1: control group that received no treatment; 2: the ndomethacin-treated group that received 20 mg/kg of indomethacin for 2 days to induce the development of gastric ulcers; 3: quercetin-treated group that in addition to the indomethacin treatment, received 50 mg/kg of quercetin 6 hours after and then daily for 14 days and; 4: the melatonin-treated group which received 20 mg/kg of melatonin 6 hours after each indomethacin treatment and then daily for 14 days. All drugs were administered orally. The following parameters were assessed in each group: mean ulcer index of gastric tissue, gastric acid volume and pH, oxidative stress markers: malondialdehyde (MDA), superoxide dismutase (SOD), glutathione peroxidase (GSH), inflammatory markers: PGE-2, TNF-α, and IL-10, nitric oxide (NO) levels and the relative gene expression of BAX, BCL-2 and COX-2 by real time PCR.Results: Our findings revealed that the indomethacin-treated group had a significantly increased (p< 0.05) ulcer index, gastric acid volume, and elevated levels of stress, inflammatory, and apoptotic markers compared to controls. In the groups that received quercetin or melatonin, these factors were all significantly decreased (p< 0.05). Between quercetin and melatonin, there was no significant difference in their gastroprotective effect.Conclusion: Both quercetin and melatonin had protective antioxidant, anti-inflammatory and antiapoptotic activity against indomethacin-induced gastric ulcers.","PeriodicalId":520763,"journal":{"name":"Reports of biochemistry & molecular biology","volume":" ","pages":"278-290"},"PeriodicalIF":1.7,"publicationDate":"2020-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7816780/pdf/rbmb-9-278.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25426870","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 12

Fabrication and Optimization of Linear PEI-Modified Crystal Nanocellulose as an Efficient Non-Viral Vector for In-Vitro Gene Delivery. 线性pei修饰晶体纳米纤维素的制备及优化作为体外基因传递的高效非病毒载体。

IF 1.7

Reports of biochemistry & molecular biology Pub Date : 2020-10-01 DOI: 10.29252/rbmb.9.3.297

Haghighat Vakilian, Eduardo Andres Rojas, Lida Habibi Rezaei, Mehrdad Behmanesh

{"title":"Fabrication and Optimization of Linear PEI-Modified Crystal Nanocellulose as an Efficient Non-Viral Vector for In-Vitro Gene Delivery.","authors":"Haghighat Vakilian, Eduardo Andres Rojas, Lida Habibi Rezaei, Mehrdad Behmanesh","doi":"10.29252/rbmb.9.3.297","DOIUrl":"https://doi.org/10.29252/rbmb.9.3.297","url":null,"abstract":"Background: One of the major challenges in gene therapy is producing gene carriers that possess high transfection efficiency and low cytotoxicity (1). To achieve this purpose, crystal nanocellulose (CNC) -based nanoparticles grafted with polyethylenimine (PEI) have been developed as an alternative to traditional viral vectors to eliminate potential toxicity and immunogenicity.Methods: In this study, CNC-PEI10kDa (CNCP) nanoparticles were synthetized and their transfection efficiency was evaluated and compared with linear cationic PEI10kDa (PEI) polymer in HEK293T (HEK) cells. Synthetized nanoparticles were characterized with AFM, FTIR, DLS, and gel retardation assays. In-vitro gene delivery efficiency by nano-complexes and their effects on cell viability were determined with fluorescent microscopy and flow cytometry.Results: Prepared CNC was oxidized with sodium periodate and its surface cationized with linear PEI. The new CNCP nano-complex showed different transfection efficiencies at different nanoparticle/plasmid ratios, which were greater than those of PEI polymer. CNPC and Lipofectamine were similar in their transfection efficiencies and effect on cell viability after transfection.Conclusion: CNCP nanoparticles are appropriate candidates for gene delivery. This result highlights CNC as an attractive biomaterial and demonstrates how its different cationized forms may be applied in designing gene delivery systems.","PeriodicalId":520763,"journal":{"name":"Reports of biochemistry & molecular biology","volume":" ","pages":"297-308"},"PeriodicalIF":1.7,"publicationDate":"2020-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7816776/pdf/rbmb-9-297.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25426871","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Recognition of (Sesc) for Easy Identification of Staphylococcus Epidermidis and Molecular and Phenotypic Study of Β-Lactam Resistance in Staphylococcus Epidermidis Isolates in Isfahan. 易鉴别表皮葡萄球菌的Sesc识别及伊斯法罕地区表皮葡萄球菌Β-Lactam耐药性的分子与表型研究

IF 1.7

Reports of biochemistry & molecular biology Pub Date : 2020-10-01 DOI: 10.29252/rbmb.9.3.309

Parisa Behshood, Elahe Tajbakhsh, Hassan Momtaz

{"title":"Recognition of (Sesc) for Easy Identification of Staphylococcus Epidermidis and Molecular and Phenotypic Study of Β-Lactam Resistance in Staphylococcus Epidermidis Isolates in Isfahan.","authors":"Parisa Behshood, Elahe Tajbakhsh, Hassan Momtaz","doi":"10.29252/rbmb.9.3.309","DOIUrl":"https://doi.org/10.29252/rbmb.9.3.309","url":null,"abstract":"Background: Not only is it crucial to rapidly detect Staphylococcus epidermidis (S. epidermidis) isolates from a broad range of bacteria, but recognizing resistance agents can greatly improve current diagnostic and therapeutic strategies.Methods: The current cross-sectional study investigated 120 clinical isolates from a nosocomial S. epidermidis infection. The isolates were identified using common biochemical tests, and specific S. epidermidis surface protein C (SesC) primers were used to confirm the presence of S. epidermidis. PCR and special primers were used to detect the β-lactamase gene (blaZ). Methicillin resistance was measured using the agar screening method and antibiotic susceptibility was measured by disk diffusion.Results: 100 samples were characterized as S. epidermidis using a phenotypic and genotypic methods. From the 100 specimens examined, 80% contained blaZ. According to agar screening, 60% of isolates were methicillin-resistant. S. epidermidis isolates demonstrated the highest resistance to penicillin (93%) and the highest sensitivity to cefazolin (39%).Conclusion: The increased resistance to β-lactam antibiotics in S. epidermidis isolates is alarming, and certain precautions should be taken by healthcare systems to continuously monitor the antimicrobial pattern of S. epidermidis, so that an appropriate drug treatment can be established.","PeriodicalId":520763,"journal":{"name":"Reports of biochemistry & molecular biology","volume":" ","pages":"309-314"},"PeriodicalIF":1.7,"publicationDate":"2020-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7816779/pdf/rbmb-9-309.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25418828","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2