Reports of biochemistry & molecular biology最新文献

{"title":"The Impact of Intermittent Hypobaric Hypoxia Exposures on Triacylglycerol Synthesis in Rat Liver.","authors":"Syarifah Dewi,&nbsp;Yulhasri Yulhasri,&nbsp;Wawan Mulyawan","doi":"10.52547/rbmb.10.3.437","DOIUrl":"https://doi.org/10.52547/rbmb.10.3.437","url":null,"abstract":"<p><strong>Background: </strong>In a hypoxic state, fatty acid breakdown reaction may be inhibited due to a lack of oxygen. It is likely that the fatty acids will be stored as triacylglycerol. The aim of this study was to analyse triacylglycerol synthesis in the liver after intermittent hypobaric hypoxia (HH) exposures.</p><p><strong>Methods: </strong>Samples are liver tissues from 25 male Wistar rats were divided into 5 groups: control group (normoxia), group I (once HH exposure), group II (twice HH exposures), group III (three-times HH exposures) and group IV (four-times HH exposures). The triacylglycerol level, mRNA expression of HIF-1α and PPAR-γ were measured in rat liver from each group.</p><p><strong>Results: </strong>We demonstrated that triacylglycerol level, mRNA expression of HIF-1α and PPAR-γ is elevated in group I significantly compared to control group. In the intermittent HH groups (group II, III and IV), mRNA expression of HIF-1α and PPAR-γ tends to downregulate near to control group. However, the triacylglycerol level is still found increased in the intermittent HH exposures groups. Significant increasing of triacylglycerol level was found especially in group IV compared to control group.</p><p><strong>Conclusion: </strong>We conclude that intermittent HH exposures will increase the triacylglycerol level in rat liver, supported by the increasing of HIF-1α and PPAR-γ mRNA expression that act as transcription factor to promote triacylglycerol synthesis.</p>","PeriodicalId":520763,"journal":{"name":"Reports of biochemistry & molecular biology","volume":" ","pages":"437-444"},"PeriodicalIF":1.7,"publicationDate":"2021-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8718788/pdf/rbmb-10-437.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39659868","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Impact of EGCG and RG108 on SOCS1 Promoter DNA Methylation and Expression in U937 Leukemia Cells.","authors":"Mohsen Alizadeh,&nbsp;Amirhossein Nafari,&nbsp;Ali Safarzadeh,&nbsp;Saeed Veiskarami,&nbsp;Mohammad Almasian,&nbsp;Ali Asghar Kiani","doi":"10.52547/rbmb.10.3.455","DOIUrl":"https://doi.org/10.52547/rbmb.10.3.455","url":null,"abstract":"<p><strong>Background: </strong>The available evidence has increasingly demonstrated that a combination of genetic and epigenetic factors, such as DNA methylation, could be considered as causing leukemia. Epigenetic changes and methylation of the suppressor of the cytokine signaling 1 promoter (SOCS1) CpG region silence SOCS1 expression in cancer. In the current study, we evaluated the impact of epigallocatechin gallate (EGCG) and RG108 on SOCS1 promoter methylation and expression in U937 cells.</p><p><strong>Methods: </strong>In the current study, U937 leukemic cells were treated with EGCG and RG108 for 12, 24, 48, and 72 h and SOCS1 promoter methylation and its expression were measured by methylation-specific PCR (MSP) and quantitative real-time PCR, respectively.</p><p><strong>Results: </strong>The outcomes indicated that the SOCS1 promoter is methylated in U937 cells, and treatment of these cells with either EGCG or RG108 reduced its methylation. Moreover, we observed that SOCS1 expression was significantly upregulated in a time-dependent manner by both EGCG and RG108 in U937 cells compared with control cells. In the RG108-treated group at 12, 24, 48, and 72 h, SOCS1 expression was upregulated by 1, 4.2, 16.6, and 32.6 -fold respectively, and in the EGCG-treated group, by 0.5, 3.2, 10.8, and 22.3 -fold, respectively.</p><p><strong>Conclusion: </strong>Treatment with either EGCG or RG108 reduced SOCS1 promoter methylation and increased SOCS1 expression in U937 cells in a time-dependent manner, which may play a role in leukemia therapy.</p>","PeriodicalId":520763,"journal":{"name":"Reports of biochemistry & molecular biology","volume":" ","pages":"455-461"},"PeriodicalIF":1.7,"publicationDate":"2021-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8718778/pdf/rbmb-10-455.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39895961","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Purification, Characterization, and Inhibition of Tyrosinase from Jerusalem Artichoke (<i>Helianthus Tuberosus</i> L.) Tuber.","authors":"Omar Younis Al-Abbasy,&nbsp;Wathba Idrees Ali,&nbsp;Aya Ihsan Rashan,&nbsp;Shihab Ahmed Al-Bajari","doi":"10.52547/rbmb.10.3.495","DOIUrl":"https://doi.org/10.52547/rbmb.10.3.495","url":null,"abstract":"<p><strong>Background: </strong>Because it tends to cause deterioration in the quality of food and appearance, food browning is unacceptable. Tyrosinase, which catalyzes the transformation of mono phenolic compounds into o-quinones, has been associated with this phenomenon. Natural anti-browning agents were used to help avoid the enzymatic browning that occurs in many foods.</p><p><strong>Methods: </strong>Tyrosinase of Jerusalem Artichoke tubers was purified through (NH4)2SO4 sedimentation, dialysis, chromatography, and finally gel electrophoresis. The purified enzyme was characterized and inhibited by rosemary extracts.</p><p><strong>Results: </strong>Purification of tyrosinase from Jerusalem Artichoke tuber were accomplished. The specific activity at the final step of purification increased to 14115.76 U/mg protein with purification fold 32.89 using CM-Cellulose chromatography. The molecular mass was evaluated by electrophoresis and found to be 62 KDa. Maximum tyrosinase activity was found at 30 °C, pH 7.2, and higher affinity towards L-tyrosine. Inhibition percentage of heated extracts for leaves and flowers on tyrosinase activity was better than nonheated with 29.65% and 23.75%, respectively. The kinetic analysis exposed uncompetitive inhibition by leaves and flowers heated extracts.</p><p><strong>Conclusion: </strong>In this study, we concluded the usage of natural anti-browning inhibitors like rosemary extract be able to be castoff to substitute the chemical agents which might be dangerous to social healthiness. Natural anti-browning agents can be used to prevent the browning of many foods.</p>","PeriodicalId":520763,"journal":{"name":"Reports of biochemistry & molecular biology","volume":" ","pages":"495-505"},"PeriodicalIF":1.7,"publicationDate":"2021-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8718770/pdf/rbmb-10-495.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39895965","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of <i>Andrographis paniculata (Burm. F.)</i> Extract on Diabetic Nephropathy in Rats.","authors":"Rachmat Hidayat,&nbsp;Patricia Wulandari","doi":"10.52547/rbmb.10.3.445","DOIUrl":"https://doi.org/10.52547/rbmb.10.3.445","url":null,"abstract":"<p><strong>Background: </strong>Hyperglycemia and accumulation of advanced glycation end products (AGEs) play a significant role in the development of diabetic nephropathy. <i>Andrographis paniculata</i> (AP) is a plant with high flavonoid content with the potential to suppress oxidative stress activity in cells and tissue. This study was aimed to investigate the role of <i>Andrographis paniculata</i> extract (APE) in protecting kidney damage due to the formation of AGEs in the renal glomerulus in diabetic rats.</p><p><strong>Methods: </strong>A total of 30 male Sprague Dawley rats were randomly divided into five groups as follows: normal control group, streptozocin (STZ) induced diabetic group, STZ-induced diabetic group with AP extract (100 mg/kg BW), STZ-induced diabetic rats with AP extract (200 mg/kg BW), and STZ-induced diabetic rats with APE (400 mg/ kg BW). Blood glucose levels were measured before treatment and after treatment. Serum and urine parameters were determined. Antioxidant enzymes and lipid peroxide levels were determined in the kidney along with histopathological examination.</p><p><strong>Results: </strong>The finding of this study showed that treatment APE at the dose of 200 mg/kg and 400 mg/kg ameliorated kidney hypertrophy index. SOD, catalase, and GSH activities significantly decreased in the kidney of STZ-diabetic rats compared to the normal control rats. Treatment with APE significantly decreased malondialdehyde level at the dose of 200 and 400 mg/kg BW.</p><p><strong>Conclusion: </strong>This study revealed evidence for improving diabetic retinopathy in male rats treated with <i>Andrographis paniculata</i> extract. APE significantly decreased oxidative stress activities in kidney of diabetic rats.</p>","PeriodicalId":520763,"journal":{"name":"Reports of biochemistry & molecular biology","volume":" ","pages":"445-454"},"PeriodicalIF":1.7,"publicationDate":"2021-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8718783/pdf/rbmb-10-445.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39895960","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Irisin and Carcinoembryonic Antigen (CEA) as Potential Diagnostic Biomarkers in Gastric and Colorectal Cancers.","authors":"Ahmed Abd Temur,&nbsp;Farah Aqeel Rashid","doi":"10.52547/rbmb.10.3.488","DOIUrl":"https://doi.org/10.52547/rbmb.10.3.488","url":null,"abstract":"<p><strong>Background: </strong>Carcinoembryonic antigen (CEA) is a common gastrointestinal tumor biomarker. Irisin is adipo-myokines that has been suggested to have a potential role in cancer development. However, limited studies test irisin as biomarker in gastric and colorectal cancers. Therefore, this study aims to investigate whether CEA and irisin could be a potential diagnostic biomarker in gastric and colorectal cancer.</p><p><strong>Methods: </strong>A case-control study consists of 90 subjects, 21 gastric cancer patients, 49 colorectal cancer patients and 20 control. Serum CEA was detected by fluorescence immunoassay (FIA) kit. Serum irisin was determined by enzyme-linked immunosorbent assay (ELISA) kit.</p><p><strong>Results: </strong>Serum CEA increases significantly and serum irisin decreases significantly in gastric and colorectal cancer patients. According to Receiver Operating Characteristic (ROC) curve analysis, in gastric cancer, the area under curve of CEA is 1.00 (95% CI, 1.000-1.000, p< 0.0001). The diagnostic cut-off of CEA is< 3.08 ng/ml with %100 sensitivity and 100% specificity. The area under curve of irisin is 0.94 (95% CI, 0.8177-1.000, p< 0.0001). The cut-off of irisin is> 30.2 ng/ml with %90 sensitivity and 100%, specificity. In colorectal cancer, the area under curve of CEA is 0.99 (95% CI, 0.9866-1.000, p< 0.0001) and the diagnostic value< 2.6 ng/ml with %98 sensitivity and %100 specificity. The area under curve of irisin is 0.96 (95% CI, 0.9155-1.000, p< 0.0001). The diagnostic cut-off of irisin is> 41.9 ng/ml with 88.1sensitivity and 90.5 specificity.</p><p><strong>Conclusion: </strong>CEA and irisin could be powerful potential diagnostic biomarkers which would be use for early detection of gastric and colorectal cancers.</p>","PeriodicalId":520763,"journal":{"name":"Reports of biochemistry & molecular biology","volume":" ","pages":"488-494"},"PeriodicalIF":1.7,"publicationDate":"2021-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8718786/pdf/rbmb-10-488.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39895964","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular and Haematological Characteristics of alpha-Thalassemia Deletions in Yogyakarta Special Region, Indonesia.","authors":"Nailil Husna,&nbsp;Niken Satuti Nur Handayani","doi":"10.52547/rbmb.10.3.346","DOIUrl":"https://doi.org/10.52547/rbmb.10.3.346","url":null,"abstract":"<p><strong>Background: </strong>alpha-Thalassemia is caused primarily by deletions of one to two alpha-globin genes and is characterized by absent or deficient production of alpha-globin protein. The South-East Asia (SEA) deletion, 3.7-kb and 4.2-kb deletions are the most common causes. The present study aimed to observe the molecular characteristics of this common alpha-Thalassemia deletions and analyse its haematological parameter.</p><p><strong>Methods: </strong>Blood samples from 173 healthy volunteers from thalassemia carrier screening in Yogyakarta Special Region were used. Haematological parameters were analysed and used to predict the carrier subjects. Genotype of suspected carriers was determined using multiplex gap-polymerase chain reaction and its haematological parameters were compared. The boundary site of each deletion was determined by analysing the DNA sequences.</p><p><strong>Results: </strong>Seventeen (9.8%) of the volunteers were confirmed to have alpha-Thalassemia trait. Of these, four genotypes were identified namely -α^3.7/αα (58.8%), -α^4.2/αα (5.9%), -α^3.7/-α^4.2 (5.9%) and - -^SEA/αα (29.4%). The 5' and 3' breakpoints of SEA deletion were located at nt165396 and nt184700 of chromosome 16, respectively. The breakpoint regions of 3.7-kb deletion were 176-bp long, whereas for 4.2-kb deletion were 321-bp long. The haematological comparison between normal and those with alpha-Thalassemia trait genotype indicated a significant difference in mean corpuscular volume (MCV) (p< 0.001) and mean corpuscular haemoglobin (MCH) (p< 0.001). As for identifying the number of defective genes, MCH parameter was more reliable (p= 0.003).</p><p><strong>Conclusion: </strong>The resultant molecular and haematological features provide insight and direction for future thalassemia screening program in the region.</p>","PeriodicalId":520763,"journal":{"name":"Reports of biochemistry & molecular biology","volume":" ","pages":"346-353"},"PeriodicalIF":1.7,"publicationDate":"2021-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8718782/pdf/rbmb-10-346.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39644296","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hepatoprotective Effects of Chitosan and Chitosan Nanoparticles against Biochemical, Genetic, and Histological Disorders Induced by the Toxicity of Emamectin Benzoate.","authors":"Sherifa Fathalla Dawoud,&nbsp;Tarek Mostafa Al-Akra,&nbsp;Amina Mohamed Zedan","doi":"10.52547/rbmb.10.3.506","DOIUrl":"https://doi.org/10.52547/rbmb.10.3.506","url":null,"abstract":"<p><strong>Background: </strong>Emamectin benzoate (EMB) is a biopesticide which used in agriculture as an insecticide. It is easier to reach ecologically and affects human health. This study aims to evaluate the protective effect of chitosan and chitosan nanoparticles against EMB-induced hepatotoxicity.</p><p><strong>Methods: </strong>Male mice were distributed into four groups: G1: the negative control, G2: EMB group (5 mg/kg diet), G3: EMB with Chitosan, (600 mg/kg diet), and G4: EMB with Chitosan nanoparticles (600 mg/kg diet). The experiment continues for 8 weeks, and the animals were sacrificed, and their organs were removed and immediately weighed after sacrifice. The liver was quickly removed and processed for histopathological and genetic studies.</p><p><strong>Results: </strong>Emamectin benzoate (EMB) treatment induced oxidative stress by increased levels of Malondialdehyde (MDA), alanine aminotransferase (ALT) and aspartate aminotransferase (AST) with inhibition of acetylcholinesterase (AChE), Superoxide dismutase (SOD) and Catalase (CAT) levels. EMB produced several histopathological changes in the liver. Relative expressions of studied genes elevated in the liver with increase in DNA damage. Co-treatment with chitosan and chitosan nanoparticles reduced EMB related liver toxicity that belong to biochemical, histopathological, gene expression, and DNA damage by increasing antioxidant capacity.</p><p><strong>Conclusion: </strong>This study offers insight into the potential for Chitosan and chitosan nanoparticles as a novel natural material against the oxidative stress induced by EMB.</p>","PeriodicalId":520763,"journal":{"name":"Reports of biochemistry & molecular biology","volume":" ","pages":"506-514"},"PeriodicalIF":1.7,"publicationDate":"2021-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8718774/pdf/rbmb-10-506.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39895966","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Expression of Vascular Endothelial Growth Factor A and Its Type 1 Receptor in Supratentorial Neoplasm.","authors":"Hamid Rezaee,&nbsp;Shadi Abbasnia,&nbsp;Anita Alenabi,&nbsp;Rosita Vakili,&nbsp;Nasrin Moheghi,&nbsp;Jalil Tavakol Afshari,&nbsp;Seyed Abdolrahim Rezaee","doi":"10.52547/rbmb.10.3.354","DOIUrl":"https://doi.org/10.52547/rbmb.10.3.354","url":null,"abstract":"<p><strong>Background: </strong>Vascular endothelial growth factor (VEGF) is one of the primary angiogenesis regulators in solid cancers. Brain solid tumors are life-threatening diseases in which angiogenesis is an important phase of tumor development and progression. In the present study, VEGF-A and VEGF receptor (VEGF-R1) gene expression was evaluated in CNS brain tumors.</p><p><strong>Methods: </strong>VEGF-A and VEGF-R1 expression was quantified using real-time PCR on fresh biopsies of 38 supratentorial brain tumors compared to 30 non-tumoral tissues. Then, the correlations were investigated with clinic-pathological and demographic factors of the patients.</p><p><strong>Results: </strong>PCR product sequencing confirmed the validity of qRT-PCR. Although VEGF-A and VEGF-R1 expression showed increasing trends with the progression of cell proliferation in different stages of astrocytoma, VEGF-R1 did not meet the 95% confidence interval in other brain tumors. An increasing trend in VEGF-A expression and a declining trend in VEGF-R1 expression from Stage I to II were observed in meningioma. VEGF-A and VEGF-R1 expression had no significant correlation with age and gender. Although peritumoral brain edema (PTBE) in astrocytoma was significantly associated with tumor stages, VEGF-A and VEGF-R1 were not correlated with PTBE in meningioma and metastasis.</p><p><strong>Conclusion: </strong>VEGF-A is a valuable factor for the prognosis of PTBE and malignancy in astrocytoma and is helpful in monitoring treatment approaches.</p>","PeriodicalId":520763,"journal":{"name":"Reports of biochemistry & molecular biology","volume":" ","pages":"354-361"},"PeriodicalIF":1.7,"publicationDate":"2021-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8718773/pdf/rbmb-10-354.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39644297","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of Nicotine on STAT1 Pathway and Oxidative Stress in Rat Lungs.","authors":"Aida Abdeen Mahmoud,&nbsp;Hekmat Osman Abdel-Aziz,&nbsp;Mohamed Elbadr,&nbsp;Hala Elbadre","doi":"10.52547/rbmb.10.3.429","DOIUrl":"https://doi.org/10.52547/rbmb.10.3.429","url":null,"abstract":"<p><strong>Background: </strong>Tobacco use is responsible for millions of preventable deaths due to cancer. Nicotine, an alkaloid chemical found in tobacco was proved to cause chronic inflammation and oxidative stress. The transcription factor STAT1 induces the expression of many proinflammatory genes and has been suggested to be a target for anti-inflammatory therapeutics. The following study investigated the effect of Nicotine on STAT1 pathway and oxidative stress in rat lung tissue.</p><p><strong>Methods: </strong>Thirty rats were divided into 3 groups; group I considered as control, group II; its rats were daily injected with Nicotine at a dose of 0.4 mg/100 gm body for 8 successive weeks and group III; its rats were daily injected with Nicotine as group II, but the injection was stopped for another 4 weeks. STAT1α protein was assessed by immunohistochemistry, COX-2 and iNOS genes expression were evaluated by real time PCR and thiobarbituric acid reactive substances (TBARS) and total thiols were measured using spectrophotometric methods in the lung tissues of the rats.</p><p><strong>Results: </strong>The results of the study revealed that group II rats had the highest expression of STAT1α protein and COX-2 and iNOS genes and oxidative stress in their lung tissues. Nicotine cessation for 4 weeks caused a marked reduction in the expression of STAT1α protein, COX-2 and iNOS genes and oxidative stress.</p><p><strong>Conclusion: </strong>Induction of STAT1 pathway and the increase in oxidative stress may be the mechanisms through which Nicotine may induce its harmful effects.</p>","PeriodicalId":520763,"journal":{"name":"Reports of biochemistry & molecular biology","volume":" ","pages":"429-436"},"PeriodicalIF":1.7,"publicationDate":"2021-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8718784/pdf/rbmb-10-429.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39659867","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular Detection of Sexually Transmitted Infections in Women with and without Human Papillomaviruses Infection Who Referred to Tehran West Hospitals in Iran.","authors":"Seyed Mojtaba Mortazavi,&nbsp;Amin Tarinjoo,&nbsp;Sepideh Dastani,&nbsp;Majid Niyazpour,&nbsp;Samira Dahaghin,&nbsp;Reza Mirnejad","doi":"10.52547/rbmb.10.3.387","DOIUrl":"https://doi.org/10.52547/rbmb.10.3.387","url":null,"abstract":"<p><strong>Background: </strong>According to the studies, many pathogens function as cofactors interacting with Human papillomavirus in the development of pre-cancer or cancer of the cervix. The aim of this study was to investigate the prevalence rate of Sexually Transmitted Infections (STIs) pathogens including <i>Mycoplasma hominis</i>, <i>Ureaplasma urealyticum</i>, <i>Chlamydia trachomatis</i>, <i>Neisseria gonorrhoeae</i>, <i>Gardnerella vaginalis</i>, and <i>Streptococcus agalactiae</i> in people with HPV and without HPV infection, and frequency rate of these pathogens in high and low risk of HPV.</p><p><strong>Methods: </strong>Cervical samples of 280 women who referred to Tehran west hospitals in Iran, between 2019 and 2020, were collected. After DNA extraction of samples, identification of HPV and genotyping was performed, and then, to detect each microorganism, the PCR was carried out with specific primers. Finally, the results were analyzed using descriptive statistics tests.</p><p><strong>Results: </strong>The mean age of patients was 37 years. Two groups of patients were identified based on positivity or negativity of HPV. In HPV-positive group (118 cases), the prevalence of <i>U. urealyticum</i>, <i>M. hominis</i>, <i>N. gonorrhoeae</i>, <i>G. vaginalis</i>, and <i>S. agalactiae</i> was 38 (13%), 7 (62%), 5.93%, 19.49%, 0.84% respectively. In HPV-negative group (162 cases), rate of infection with <i>U. urealyticum</i>, <i>M. hominis</i>, <i>N. gonorrhoeae</i>, <i>G. vaginalis</i>, and <i>S. agalactiae</i> was 29.62%, 6.17%, 3.08%, 16.04%, 0.61% respectively. Among the two groups, there was only 1 patient with C. trachomatis (0.84%), seen in HPV-positive group.</p><p><strong>Conclusion: </strong>In this study no significant association was found between HPV and bacteria such as G. vaginalis and S. agalactiae, and it was found that C. trachomatis, and especially N. gonorrhoeae are strongly associated with HPV infection.</p>","PeriodicalId":520763,"journal":{"name":"Reports of biochemistry & molecular biology","volume":" ","pages":"387-395"},"PeriodicalIF":1.7,"publicationDate":"2021-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8718787/pdf/rbmb-10-387.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39659862","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}