Proceedings. Biological sciences最新文献

A marine stem-myriapod from the Silurian Waukesha Lagerstätte, Wisconsin, USA: terrestrial traits pre-date the transition to land. 来自美国威斯康辛州志留纪Waukesha Lagerstätte的一种海洋茎多足动物：在向陆地过渡之前的陆地特征。

IF 3.5

Proceedings. Biological sciences Pub Date : 2026-05-06 DOI: 10.1098/rspb.2026.0131

Derek E G Briggs, James C Lamsdell, Joanne Kluessendorf, Donald G Mikulic

{"title":"A marine stem-myriapod from the Silurian Waukesha Lagerstätte, Wisconsin, USA: terrestrial traits pre-date the transition to land.","authors":"Derek E G Briggs, James C Lamsdell, Joanne Kluessendorf, Donald G Mikulic","doi":"10.1098/rspb.2026.0131","DOIUrl":"https://doi.org/10.1098/rspb.2026.0131","url":null,"abstract":"A new aquatic myriapod-like arthropod, Waukartus muscularis, from the Silurian (Llandoverian) Waukesha marine Lagerstätte of Wisconsin with uniramous limbs also preserves details of muscle tissue and endoskeleton. Phylogenetic analyses place it in a position just stemward of crown-group myriapods, sister taxon to a clade comprising euthycarcinoids and Myriapoda, providing evidence of the ancestral morphology of the living clade. A number of key myriapod synapomorphies, including the presence of an intercalary segment with a surmised tritocerebral appendage, and the decoupling of trunk tergite and appendage expression, evolved in euthycarcinoids within the stem lineage but crownward of the Waukesha myriapod. The new taxon is an aquatic member of this stem lineage and demonstrates that the loss of exopods, which results in uniramous limbs, pre-dated the common ancestor of the amphibious euthycarcinoids and terrestrial myriapods and does not represent an adaptation to terrestrial locomotion. The Waukesha Lagerstätte is unusual among Silurian localities in preserving taxa more typical of Cambrian communities alongside those characteristic of the post-Cambrian.","PeriodicalId":520757,"journal":{"name":"Proceedings. Biological sciences","volume":"293 2070","pages":""},"PeriodicalIF":3.5,"publicationDate":"2026-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147849205","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Heritable transgenerational fitness variation correlates with copper resistance in the clonal duckweed Spirodela polyrhiza. 无性系浮萍（Spirodela polyrhiza）的遗传跨代适应性变异与铜抗性相关。

IF 3.5

Proceedings. Biological sciences Pub Date : 2026-05-06 DOI: 10.1098/rspb.2025.3304

Alexandra Chávez, Martin Schäfer, Iris Finkemeier, Shuqing Xu, Meret Huber

{"title":"Heritable transgenerational fitness variation correlates with copper resistance in the clonal duckweed Spirodela polyrhiza.","authors":"Alexandra Chávez, Martin Schäfer, Iris Finkemeier, Shuqing Xu, Meret Huber","doi":"10.1098/rspb.2025.3304","DOIUrl":"https://doi.org/10.1098/rspb.2025.3304","url":null,"abstract":"Transgenerational effects can vary among genotypes of a given species; however, we cannot yet predict the direction and magnitude of the transgenerational effects. Here, we explored whether a genotype's transgenerational fitness effects correlate with plant resistance and the production of specialized metabolites. Thus, we exposed monoclonal, single-descendant lineages from 56 globally distributed genotypes of the clonal duckweed Spirodela polyrhiza to five generations of control conditions or copper excess, followed by five generations without stress, and then measured plant fitness and phenotypes in both environments. Previous copper excess reduced fitness variation within genotypes, while generating heritable and repeatable differences in transgenerational fitness effects among genotypes: genotypes in which previous stress increased fitness under recurring conditions in comparison to controls tended to show reduced fitness when the stress was absent, and vice versa. The transgenerational fitness effects correlated with copper resistance: the more susceptible a genotype, the more its offspring suffered from ancestral stress in the absence of recurrent stress; however, these offspring tended to be more resistant to copper excess, likely by protecting photosystem II. Together, these findings show that transgenerational fitness effects are heritable, variable and can be anticipated from stress resistance.","PeriodicalId":520757,"journal":{"name":"Proceedings. Biological sciences","volume":"293 2070","pages":""},"PeriodicalIF":3.5,"publicationDate":"2026-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147849223","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A true Luciolinae fossil from the mid-Cretaceous Burmese amber provides new insights into the early evolution of fireflies (Coleoptera: Lampyridae). 从白垩纪中期缅甸琥珀中发现的一具真正的萤科化石，为了解萤火虫的早期进化提供了新的见解。

IF 3.5

Proceedings. Biological sciences Pub Date : 2026-05-06 DOI: 10.1098/rspb.2025.3316

Shuailong Yuan, Haoyu Liu, Sining Lv, Yuxia Yang

{"title":"A true Luciolinae fossil from the mid-Cretaceous Burmese amber provides new insights into the early evolution of fireflies (Coleoptera: Lampyridae).","authors":"Shuailong Yuan, Haoyu Liu, Sining Lv, Yuxia Yang","doi":"10.1098/rspb.2025.3316","DOIUrl":"https://doi.org/10.1098/rspb.2025.3316","url":null,"abstract":"Fireflies (Lampyridae) represent the most diverse terrestrial group of organisms capable of bioluminescence. However, their evolution remains inadequately understood due to the scarcity of early fossils. In this study, we uncover a new fossil genus and species, Cretoluciola birmana gen. et sp. nov., derived from a high-quality piece of the mid-Cretaceous Burmese amber. This new taxon is characterized by large eyes, filiform antennae, six abdominal ventrites and a bipartite light organ in males. It is robustly placed in the subfamily Luciolinae based on both maximum parsimony and Bayesian inference analyses (bootstrap results, with support values (BS) = 99.7%; posterior probabilities (PP) = 1.00), incorporating 410 morphological characters from 37 extant lampyrid genera plus the fossil taxon, and eight AHE loci from representative species. As the first definitive Cretaceous fossil, it establishes a minimum age constraint of 99 million years for Luciolinae, the crown group of Lampyridae. However, these findings cast doubt on the systematic placements of the other two fossil genera within Luciolinae, Flammarionella and Protoluciola, suggesting that the mid-Cretaceous Burmese fauna may have exhibited greater firefly diversity than previously recognized, at least at the subfamilial level. Furthermore, early fireflies are supposed to be nocturnal and adopt various survival and courtship strategies, thereby enhancing our understanding of the evolution of Lampyridae.","PeriodicalId":520757,"journal":{"name":"Proceedings. Biological sciences","volume":"293 2070","pages":""},"PeriodicalIF":3.5,"publicationDate":"2026-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147849249","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Life-history strategy mediates the effects of multiple developmental stressors on Australian lizards. 生活史策略介导多种发育应激源对澳大利亚蜥蜴的影响。

IF 3.5

Proceedings. Biological sciences Pub Date : 2026-05-06 DOI: 10.1098/rspb.2026.0243

Dalton C Leibold, Pablo Recio, Amelia Y Peardon, Ondi L Crino, Christopher Friesen, Daniel W A Noble

{"title":"Life-history strategy mediates the effects of multiple developmental stressors on Australian lizards.","authors":"Dalton C Leibold, Pablo Recio, Amelia Y Peardon, Ondi L Crino, Christopher Friesen, Daniel W A Noble","doi":"10.1098/rspb.2026.0243","DOIUrl":"https://doi.org/10.1098/rspb.2026.0243","url":null,"abstract":"Maternal stress and the developmental environment jointly affect offspring fitness and phenotype during early life. Mothers transmit stress to offspring through the deposition of hormones and energy into developing embryos, who are themselves subject to environmental stressors. A species' life-history strategy (e.g. size and number of eggs) may further buffer or exacerbate the effects of developmental stress on offspring. We experimentally tested the joint effects of stress hormones (exogenous corticosterone applied directly to eggs) and the developmental environment (incubation temperature) on the hatching success and size of two lizards (Lampropholis delicata and Lampropholis guichenoti) that differ in maternal investment strategy. Hatching success was reduced by corticosterone exposure, but only in L. delicata, which produces clutches of more numerous but smaller eggs than L. guichenoti. Hatchling size in both species, however, was reduced by corticosterone exposure, incubation at high temperatures or both. In both species, maternal investment (egg size) was the best predictor of hatching success and hatchling size, regardless of treatment. Our findings suggest that, both within species and between species, greater maternal investment buffers offspring from maternal and developmental stress effects on early-life fitness (survival) but does not prevent their effects on fitness-associated phenotypes (hatchling size).","PeriodicalId":520757,"journal":{"name":"Proceedings. Biological sciences","volume":"293 2070","pages":""},"PeriodicalIF":3.5,"publicationDate":"2026-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147849241","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The ray-finned fish blackbox: unprecedented morphological diversity and the interplay between brain and endocast. 鳍鱼黑箱：前所未有的形态多样性和脑与内质间的相互作用。

IF 3.5

Proceedings. Biological sciences Pub Date : 2026-05-06 DOI: 10.1098/rspb.2025.3277

Rodrigo Tinoco Figueroa, Stephanie E Pierce

{"title":"The ray-finned fish blackbox: unprecedented morphological diversity and the interplay between brain and endocast.","authors":"Rodrigo Tinoco Figueroa, Stephanie E Pierce","doi":"10.1098/rspb.2025.3277","DOIUrl":"https://doi.org/10.1098/rspb.2025.3277","url":null,"abstract":"Vertebrates are unique among animals as they bear a neurocranium, which almost completely encapsulates the brain. Thus, within vertebrates, the evolution of neuroanatomical features might be tightly correlated with the evolution of their surrounding neurocranial ossifications, as these pose spatial constraints to brain size and shape. The endocranial cast, or surrounding neurocranial ossifications, poses spatial constraints to brain size and shape. The endocranial cast, or 'endocast', represents an important structure for the study of vertebrate neuroanatomical evolution as it has been considered to be closely associated with brain morphology. This assumption has been tested for many extant vertebrate lineages, including archosaurs, mammals and non-tetrapod sarcopterygian fishes, but brain-endocast correlation has never been studied for ray-finned fishes. Given that ray-finned fishes represent roughly half of extant vertebrate diversity and show remarkable morphological disparity, understanding the relationship between brain and endocast in this major vertebrate lineage becomes paramount for understanding vertebrate neurocranial evolution. Here, we describe previously unknown morphological diversity of endocasts in extant ray-finned fishes, compare it to endocasts from the fossil record and explore the relationship between endocast and brain across a large sample of extant taxa. Our results indicate that endocasts are a poor predictor of brain morphology for ray-finned fishes but can provide valuable information regarding neurocranial diversification and its ties to ecological features.","PeriodicalId":520757,"journal":{"name":"Proceedings. Biological sciences","volume":"293 2070","pages":""},"PeriodicalIF":3.5,"publicationDate":"2026-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147849244","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Geographic divergence in population genomics and shell morphology reveal history of glacial refugia in a coastal dogwhelk. 种群基因组学和壳形态的地理差异揭示了沿海狗螺的冰川避难史。

IF 3.5

Proceedings. Biological sciences Pub Date : 2026-05-06 DOI: 10.1098/rspb.2025.3148

Emily K Longman, Joaquin C B Nunez, Eric Sanford, Melissa H Pespeni

{"title":"Geographic divergence in population genomics and shell morphology reveal history of glacial refugia in a coastal dogwhelk.","authors":"Emily K Longman, Joaquin C B Nunez, Eric Sanford, Melissa H Pespeni","doi":"10.1098/rspb.2025.3148","DOIUrl":"https://doi.org/10.1098/rspb.2025.3148","url":null,"abstract":"Studying contemporaneous spatial patterns of genomic diversity can yield important insights into the evolutionary processes that structure populations and shape patterns of adaptation. In contrast to the large number of marine species with planktonic larvae, populations of marine taxa with low dispersal and deep evolutionary divergences offer an opportunity to reveal the phylogeographic histories of marine ecosystems. Here, we constructed a draft genome assembly for the low-dispersing marine dogwhelk, Nucella canaliculata, and studied patterns of genomic diversity and shell morphometrics in 19 populations distributed along approximately 1500 km of the west coast of North America. We observed significant population structure with a strong phylogeographic break at Monterey Bay, California, which was matched with divergence in shell morphology. Genomic patterns, concomitant with computer simulations, suggest that there were at least two refugial populations during the last glacial maximum that subsequently experienced post-glacial expansion and admixture. Lastly, linking genotype to phenotype, we identified candidate loci underlying variation in shell morphology. These findings demonstrate how high-resolution genomic data reveal the roles of phylogeography, selection and historical events in shaping the spatial distribution of genetic variation, offering key insights into the processes that structure modern coastal populations and their potential to respond to future climatic changes.","PeriodicalId":520757,"journal":{"name":"Proceedings. Biological sciences","volume":"293 2070","pages":""},"PeriodicalIF":3.5,"publicationDate":"2026-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147849266","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Felid distributions in the Southern Yungas: enhancing species distribution models with field data. 云南南部猫科动物分布：用野外数据增强物种分布模型。

IF 3.5

Proceedings. Biological sciences Pub Date : 2026-05-06 DOI: 10.1098/rspb.2025.2575

Griet An Erica Cuyckens, Pablo Gaston Perovic, Marcelo F Tognelli, L R Malizia

{"title":"Felid distributions in the Southern Yungas: enhancing species distribution models with field data.","authors":"Griet An Erica Cuyckens, Pablo Gaston Perovic, Marcelo F Tognelli, L R Malizia","doi":"10.1098/rspb.2025.2575","DOIUrl":"https://doi.org/10.1098/rspb.2025.2575","url":null,"abstract":"Biodiversity assessments in remote ecosystems are often limited by the difficulty of detecting rare, cryptic or nocturnal species. Species distribution models (SDMs) are widely used to predict distributions, yet few studies validate them with independent field data. Converting continuous suitability maps into binary predictions through threshold selection remains controversial. Nearly 50% of felid species are threatened, making accurate habitat prediction essential for conservation. The Yungas ecoregion in northwest Argentina is a biodiversity hotspot where felid distributions are poorly understood. This study tests SDMs as a conservation tool and empirically improves their robustness. We generated distribution maps for felids in the Yungas sensu stricto using MaxEnt and existing presence records, followed by a camera-trap survey for independent validation. A novel field-based threshold was used to produce a species richness map. Model performance was evaluated at site and species levels, with predictions tested using the Mann-Whitney test. High-probability areas successfully predicted the presence of ocelot (Leopardus pardalis), tiger cat (Leopardus pardinoides) and margay (Leopardus wiedii). The field-based threshold was effective at both ecoregional and site scales, though threatened species were underrepresented. Integrating SDMs with independent field data and empirical thresholding improves biodiversity assessments of elusive species and can guide conservation in other cryptic or data-deficient taxa.","PeriodicalId":520757,"journal":{"name":"Proceedings. Biological sciences","volume":"293 2070","pages":""},"PeriodicalIF":3.5,"publicationDate":"2026-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147849303","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dispersal evolution in a population infected with a male-killing endosymbiont. 种群中被杀死雄性的内共生体感染的扩散进化。

IF 3.5

Proceedings. Biological sciences Pub Date : 2026-05-06 DOI: 10.1098/rspb.2026.0004

Franziska A Brenninger, Simon H Martin, Hanna Kokko

{"title":"Dispersal evolution in a population infected with a male-killing endosymbiont.","authors":"Franziska A Brenninger, Simon H Martin, Hanna Kokko","doi":"10.1098/rspb.2026.0004","DOIUrl":"https://doi.org/10.1098/rspb.2026.0004","url":null,"abstract":"Host dispersal influences how frequently a parasite encounters new susceptible hosts, which translates into potential conflict between parasite and host interests regarding dispersal. In vertically transmitted parasites, the impact of host dispersal on parasite spread appears limited. Here, it is less intuitive whether parasites can benefit by manipulating host dispersal and, if so, whether they would evolve to increase or decrease host dispersal. We examine host-parasite conflict over dispersal using the case of the African monarch butterfly (Danaus chrysippus) and the male-killing Spiroplasma as inspiration. Male-killing endosymbionts transmit vertically from host females to their offspring and infected male offspring die. We contrast spread of a male-killing endosymbiont with a hypothetical scenario in which the parasite can additionally manipulate host dispersal. We show that a dispersal-manipulating male killer can switch the system from an overall male-biased dispersal pattern to a female-biased one. Interestingly, while infected females disperse most, the strongest evolutionary response occurs in uninfected females, which evolve towards philopatry (relative to a scenario where parasites cannot manipulate hosts). Our work thus uncovers a novel context where the phenotypic effect of parasitism is strongest in individuals that are not at risk of ever being parasitized.","PeriodicalId":520757,"journal":{"name":"Proceedings. Biological sciences","volume":"293 2070","pages":""},"PeriodicalIF":3.5,"publicationDate":"2026-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147849228","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Repeated evolutionary turnover of vertebrate skeletal muscle myosins. 脊椎动物骨骼肌肌球蛋白的重复进化转换。

IF 3.5

Proceedings. Biological sciences Pub Date : 2026-05-06 DOI: 10.1098/rspb.2026.0254

Christina M Harvey, Eric R Schuppe, Michael S Brainard, Matthew J Fuxjager, James B Pease

{"title":"Repeated evolutionary turnover of vertebrate skeletal muscle myosins.","authors":"Christina M Harvey, Eric R Schuppe, Michael S Brainard, Matthew J Fuxjager, James B Pease","doi":"10.1098/rspb.2026.0254","DOIUrl":"https://doi.org/10.1098/rspb.2026.0254","url":null,"abstract":"Myosin heavy chain proteins are essential for muscle contraction and nearly every physiological function in animals, but their diversity and evolution outside mammals are largely unknown. We comprehensively model the evolutionary history of 1201 heavy-chain myosins from across Chordata. We find that skeletal muscle myosins are located in a conserved tandem gene array in all vertebrate species, but repeated gene duplication-loss turnover has surprisingly led to an independently evolved set of core skeletal muscle myosins in each major vertebrate group. Despite these separate derivations of these myosin subfamilies, each major vertebrate group exhibits tissue-specific patterns of subfamily expression and specialized myosin subfamily expression in extreme muscles. Our results show that muscle evolution across vertebrates is not based on conserved one-to-one orthologous motor myosins, as might be expected for such a core structural protein family. Instead, we find that skeletal muscle myosins have evolved as a shifting cluster of genes that is constantly changing and diversifying to balance maintainance of core physiological processes and innovation of new physiological possibilities.","PeriodicalId":520757,"journal":{"name":"Proceedings. Biological sciences","volume":"293 2070","pages":""},"PeriodicalIF":3.5,"publicationDate":"2026-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147849239","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mechanisms and control of a novel vocalization: the singing mouse song is a whistle that depends on air sac inflation. 一种新的发声机制和控制：老鼠的歌声是一种哨子，依赖于气囊的膨胀。

IF 3.5

Proceedings. Biological sciences Pub Date : 2026-05-06 DOI: 10.1098/rspb.2026.0153

Samantha Khouri Smith, Jonas Håkansson, Paul W Frazel, Michael Long, Coen P H Elemans, Steven M Phelps

{"title":"Mechanisms and control of a novel vocalization: the singing mouse song is a whistle that depends on air sac inflation.","authors":"Samantha Khouri Smith, Jonas Håkansson, Paul W Frazel, Michael Long, Coen P H Elemans, Steven M Phelps","doi":"10.1098/rspb.2026.0153","DOIUrl":"https://doi.org/10.1098/rspb.2026.0153","url":null,"abstract":"Vocalizations are ancient behaviours that require the complex coordination of breath and display. Understanding how laryngeal anatomy shapes vocalization provides insights into this diversity, its mechanisms and their evolution. Rodents are ideal for exploring this variation because of their diverse mechanisms and vocal structures. Here, we describe the laryngeal morphology and sound production mechanisms underlying the vocalizations of Alston's singing mouse (Scotinomys teguina) and compare these results to those of other vocalizing mammals. We reconstructed the three-dimensional laryngeal morphology with micro-computed tomography, recorded laryngeal sound production using high-speed video and investigated frequency control using surgical ablations. We found that singing mice use a whistle mechanism that uniquely relies on the inflation of an enlarged air sac called the ventral pouch. Song frequency can be controlled by pouch volume, airflow and cricothyroid muscle action. Singing mouse laryngeal morphology and vocal mechanism are distinct from those of other Neotomids; singing mice appear to use inflation-mediated whistles for both distant and close exchanges. Inflatable air sacs have evolved repeatedly for sound modulation and filtering. Our results indicate a novel role for these structures in being required to generate sound. Together, our results expand on an emerging story of how biomechanic and morphological variation contributes to vocal diversity.","PeriodicalId":520757,"journal":{"name":"Proceedings. Biological sciences","volume":"293 2070","pages":""},"PeriodicalIF":3.5,"publicationDate":"2026-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147849310","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0