Journal of investigative medicine : the official publication of the American Federation for Clinical Research最新文献_第6页

Risk factors associated with adenocarcinoma development in gastric neuroendocrine tumors: A multicenter 10-year follow-up study. 表达：胃神经内分泌肿瘤中与腺癌发展相关的危险因素：一项多中心10年随访研究

Journal of investigative medicine : the official publication of the American Federation for Clinical Research Pub Date : 2025-06-03 DOI: 10.1177/10815589251348926

Cagdas Kalkan, Fatih Acehan, Ali Atay, Ali Karatas, Erol Aksoy, Kerem Kenarli, Emra Asfuroglu Kalkan, Tarkan Karakan, Murat Kekilli, Mehmet Cindoruk, Irfan Soykan

{"title":"Risk factors associated with adenocarcinoma development in gastric neuroendocrine tumors: A multicenter 10-year follow-up study.","authors":"Cagdas Kalkan, Fatih Acehan, Ali Atay, Ali Karatas, Erol Aksoy, Kerem Kenarli, Emra Asfuroglu Kalkan, Tarkan Karakan, Murat Kekilli, Mehmet Cindoruk, Irfan Soykan","doi":"10.1177/10815589251348926","DOIUrl":"10.1177/10815589251348926","url":null,"abstract":"There is a significant gap in the literature regarding the relationship between gastric neuroendocrine tumors (GNETs) and gastric adenocarcinoma. This study aimed to examine the incidence and risk factors of adenocarcinoma development in patients diagnosed with GNET. This study included patients who were diagnosed with GNET and were followed up for a maximum period of 120 months via annual endoscopy. The patients were divided into two groups according to whether they developed adenocarcinoma or not during their follow-up period. These two groups were compared in terms of histopathological and laboratory findings, and risk factors associated with adenocarcinoma were identified. The Kaplan-Meier method was used to estimate the cumulative probability of adenocarcinoma, considering the total number of risk factors. Out of the 107 patients included in the study, 25 (23.3%) developed adenocarcinoma during follow-up, with an incidence rate of 3.87 events per 100 person-years. The median follow-up period of patients in both groups was 72 months. Severe corpus atrophy, micronodular enterochromaffin-like cell hyperplasia, presence of anti-Helicobacter pylori antibodies, hemoglobin, vitamin B12, ferritin, and gastrin were found to be risk factors for adenocarcinoma. For adenocarcinoma, a cut-off value of four for the total number of risk factors yielded a sensitivity of 92.0% and a specificity of 92.7%. The risk of developing adenocarcinoma was approximately 15 times (hazard ratio: 15.6, 95% confidence interval: 3.6-66.5) higher in patients with at least three risk factors. By combining seven basic histopathological and laboratory findings, the development of adenocarcinoma in patients with GNET was successfully predicted.","PeriodicalId":520677,"journal":{"name":"Journal of investigative medicine : the official publication of the American Federation for Clinical Research","volume":" ","pages":"10815589251348926"},"PeriodicalIF":0.0,"publicationDate":"2025-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144210734","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Predictive value of albumin-corrected anion gap for acute kidney injury risk assessment in sepsis: Insights from MIMIC-IV. EXPRESS：白蛋白校正阴离子间隙对脓毒症急性肾损伤风险评估的预测价值：来自MIMIC-IV的见解。

Journal of investigative medicine : the official publication of the American Federation for Clinical Research Pub Date : 2025-06-03 DOI: 10.1177/10815589251348930

Jing Nie, Shengyao Qi

{"title":"Predictive value of albumin-corrected anion gap for acute kidney injury risk assessment in sepsis: Insights from MIMIC-IV.","authors":"Jing Nie, Shengyao Qi","doi":"10.1177/10815589251348930","DOIUrl":"10.1177/10815589251348930","url":null,"abstract":"Acute kidney injury (AKI) represents a major cause of death among patients with sepsis. While recent evidence suggests that albumin-corrected anion gap (ACAG) might serve as an early indicator of AKI, its prognostic capabilities require further investigation. We conducted a retrospective analysis of critical care data from the Medical Information Mart for Intensive Care IV electronic health record repository. Restricted cubic splines (RCSs) and Cox proportional hazards models were employed to quantify ACAG's association with AKI occurrence and in-hospital mortality. Kaplan-Meier survival analyses were performed to compare outcomes across ACAG-based groups, and subgroup analyses were carried out to evaluate the robustness of these associations and potential interactions between variables. Among 19,445 included patients, elevated ACAG emerged as a strong predictor of AKI (hazard ratio, HR (95% confidence interval, CI): 16.75 (14.50, 19.75), p < 0.001) and in-hospital mortality (HR (95% CI): 16.75 (14.50, 19.75), p < 0.001). RCS analysis showed a predominantly linear relationship between ACAG and clinical outcomes (AKI: p for nonlinear = 0.059; mortality: p for nonlinear = 0.794), with 17 emerging as a critical ACAG threshold value. Significant interactions were identified between ACAG and factors such as sex, age, and chronic kidney disease status. Our findings demonstrate ACAG's robust ability to predict adverse outcomes in sepsis, particularly regarding kidney function deterioration and survival. These findings highlight the potential clinical utility of ACAG as a prognostic marker to guide early therapeutic interventions.","PeriodicalId":520677,"journal":{"name":"Journal of investigative medicine : the official publication of the American Federation for Clinical Research","volume":" ","pages":"10815589251348930"},"PeriodicalIF":0.0,"publicationDate":"2025-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144210733","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Polymorphism of Angiotensin-Converting Enzyme 2 Gene (rs 2285666) and its susceptibility to COVID-19 infection and severity of the disease. 血管紧张素转换酶2基因（rs2285666）多态性及其对新冠肺炎感染和病情严重程度的易感性

Journal of investigative medicine : the official publication of the American Federation for Clinical Research Pub Date : 2025-06-03 DOI: 10.1177/10815589251348921

Hend Samir Abdelkader Elshewaikh, Gihan Farouk Attia, Wesam Salah Mohamed Ibrahim, Ragia Samir Sharshar, Dina A Ali

{"title":"Polymorphism of Angiotensin-Converting Enzyme 2 Gene (rs 2285666) and its susceptibility to COVID-19 infection and severity of the disease.","authors":"Hend Samir Abdelkader Elshewaikh, Gihan Farouk Attia, Wesam Salah Mohamed Ibrahim, Ragia Samir Sharshar, Dina A Ali","doi":"10.1177/10815589251348921","DOIUrl":"10.1177/10815589251348921","url":null,"abstract":"Single-nucleotide polymorphisms may alter severe acute respiratory syndrome virus 2 (SARS-CoV-2) binding or entry and increase lung or other organ tissue damage in the most prevalent genes, angiotensin-converting enzyme 2 (ACE2) and its close relative, ACE1. The goal of this study was to look at the severity of the illness, the involvement of the ACE2 gene polymorphism, and its vulnerability to coronavirus disease 19 (COVID-19) infection. Forty patients, both male and female, with a diagnosis of COVID-19, ages 25 to 70, participated in this retrospective case-control research, which was divided into two groups (Group II: diagnosed as mild COVID-19, and Group III: diagnosed with severe COVID-19) and 20 individuals as a control group. Compared to groups II and I, group III's ACE2 serum level was considerably lower (p < 0.05). Ferritin, D-dimer, procalcitonin, interleukin-6 (IL-6), and fibrinogen all showed a significant negative connection (p < 0.05). Significant positive correlations were found with oxygen saturation and relative lymphocyte presence (p < 0.05). Serum ACE2 and ferritin levels can significantly predict patients with SARS-CoV-2, respectively, at cutoff values of 23 and 75, with 90% and 92% sensitivity, 85% and 90% specificity. C-reactive protein, ferritin, procalcitonin, IL-6, and D-dimer showed a significant increase in patients with the GG genotype than the AA and GA genotypes in groups II and III (p < 0.05). ACE2 is the primary receptor for SARS-CoV-2 entrance into many bodily cells and is crucial to the pathophysiology of COVID-19. It may be a useful biomarker for assessing the risk of acquiring SARS-CoV-2, as demonstrated by the fact that COVID-19 patients had much lower blood levels of the virus than healthy controls.","PeriodicalId":520677,"journal":{"name":"Journal of investigative medicine : the official publication of the American Federation for Clinical Research","volume":" ","pages":"10815589251348921"},"PeriodicalIF":0.0,"publicationDate":"2025-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144210732","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Key inflammatory mediators drive the resolution of acute Lyme disease. EXPRESS：关键炎症介质驱动急性莱姆病的解决。

Journal of investigative medicine : the official publication of the American Federation for Clinical Research Pub Date : 2025-06-03 DOI: 10.1177/10815589251348934

Jorge Cervantes, Shahrukh Chaudhry, Jose Barragan, Idris Akinlusi, Ted Zhao, Ryan Graff, Bo-Young Hong

{"title":"Key inflammatory mediators drive the resolution of acute Lyme disease.","authors":"Jorge Cervantes, Shahrukh Chaudhry, Jose Barragan, Idris Akinlusi, Ted Zhao, Ryan Graff, Bo-Young Hong","doi":"10.1177/10815589251348934","DOIUrl":"10.1177/10815589251348934","url":null,"abstract":"Lyme disease is an inflammatory disease in response to Borrelia burgdorferi infection. We analyzed cytokines in sera from acute and convalescent LD patients and also evaluated the effect of these mediators on human monocytes. Different inflammatory mediators were measured in human sera obtained during active disease and convalescence, as well as from healthy controls. IL-12, IFN-gamma, and soluble TNF receptors were elevated in acute LD sera, which returned to normal levels during convalescence. MMP-1 levels, however, continued to be elevated. Convalescence sera showed a decrease in the secretion of sIL6R, IL-26, IL-2, and IFN-alpha, which were also elevated in acute LD but dropped during convalescence. Decreased levels of anti-inflammatory, Th2- and M2 polarization-associated cytokines sCD163, IFN-β, and IL-10 did not change during acute LD but decreased during convalescence. As multiple pathways are turned on and off during acute and convalescence, we performed a serum transcriptomics approach on human monocytes exposed to these sera. Differential expression of proinflammatory genes, endosomal proteins, type I IFN-related genes, and interferon-dependent genes was observed. In cells exposed to convalescent sera, genes such as CXCL3 and CCL20 did not return to their baseline levels. Our results help to elaborate a better understanding of the inflammatory processes involved in the progression of acute and the resolution of LD.","PeriodicalId":520677,"journal":{"name":"Journal of investigative medicine : the official publication of the American Federation for Clinical Research","volume":" ","pages":"10815589251348934"},"PeriodicalIF":0.0,"publicationDate":"2025-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144210731","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pseudomonas aeruginosa extracellular vesicles affect gene regulation and lung inflammation and immunity in cystic fibrosis. 表达：铜绿假单胞菌胞外囊泡影响囊性纤维化患者的基因调控和肺部炎症免疫。

Journal of investigative medicine : the official publication of the American Federation for Clinical Research Pub Date : 2025-05-30 DOI: 10.1177/10815589251348918

Andrea Hahn, Kylie I Krohmaly, Aszia Burrell, Junfeng Ma, Brennan Harmon, Claire Hoptay, Robert J Freishtat

{"title":"Pseudomonas aeruginosa extracellular vesicles affect gene regulation and lung inflammation and immunity in cystic fibrosis.","authors":"Andrea Hahn, Kylie I Krohmaly, Aszia Burrell, Junfeng Ma, Brennan Harmon, Claire Hoptay, Robert J Freishtat","doi":"10.1177/10815589251348918","DOIUrl":"10.1177/10815589251348918","url":null,"abstract":"Bacterial extracellular vesicles (EVs) are important mediators of host infection. People with cystic fibrosis (CF) often suffer from chronic infection with Pseudomonas aeruginosa, an opportunistic pathogen. However, the relative abundance of P. aeruginosa is not associated with the onset of increased pulmonary symptoms, known as a pulmonary exacerbation. We hypothesized that the cargo of P. aeruginosa EVs is different at times of baseline wellness and pulmonary exacerbation onset in persons with CF. This is the first study to characterize and compare P. aeruginosa EVs at these two time points, using a novel series of steps to isolate the P. aeruginosa EVs directly from the sputum of persons with CF. Our study found a differential packaging of P. aeruginosa proteins at baseline wellness and pulmonary exacerbation, with six proteins being more frequently present in pulmonary exacerbation samples. In addition, we were able to demonstrate that the P. aeruginosa EVs isolated from the sputum of persons with CF at the time of pulmonary exacerbation induced an inflammatory response in CF human bronchial epithelial cells. These data, while preliminary, support the clinical relevance of P. aeruginosa EVs in influencing gene regulation and lung inflammation and immunity in persons with CF.","PeriodicalId":520677,"journal":{"name":"Journal of investigative medicine : the official publication of the American Federation for Clinical Research","volume":" ","pages":"10815589251348918"},"PeriodicalIF":0.0,"publicationDate":"2025-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144188792","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nasopharyngeal airway-assisted sedation enhances oxygenation and reduces perioperative adverse events in pediatric dental surgery: A comparative clinical study. 一项比较临床研究：鼻咽气道辅助镇静增强氧合并减少儿童牙科手术围手术期不良事件。

Journal of investigative medicine : the official publication of the American Federation for Clinical Research Pub Date : 2025-05-30 DOI: 10.1177/10815589251348914

Yuyan Sun, Qian Li

{"title":"Nasopharyngeal airway-assisted sedation enhances oxygenation and reduces perioperative adverse events in pediatric dental surgery: A comparative clinical study.","authors":"Yuyan Sun, Qian Li","doi":"10.1177/10815589251348914","DOIUrl":"10.1177/10815589251348914","url":null,"abstract":"Perioperative airway management during pediatric dental procedures remains challenging, particularly in balancing oxygenation stability with procedural safety under intravenous sedation. This randomized trial compared nasopharyngeal airway (NPA)-assisted intravenous sedation (n = 30) versus traditional nasal cannula oxygenation (n = 30) in 60 children aged 6-12 years undergoing dental surgery. Both groups received standardized midazolam-propofol sedation and perioperative monitoring. Primary outcomes encompassed oxygen saturation (SpO2), adverse events (aspiration, choking, mucosal bleeding), sedation efficacy, and surgeon satisfaction. The NPA group demonstrated superior SpO2 maintenance at key procedural stages (p < 0.05), with a 78% reduction in hypoxemia episodes compared to controls. Notably, aspiration and choking incidents decreased significantly (p < 0.05), yielding a fourfold lower overall adverse event rate (6.67% vs 30.00%, p = 0.001). Sedation quality metrics revealed enhanced behavioral cooperation (p < 0.001) and optimal sedation depth (Ramsay scores: p < 0.001) in the NPA cohort, though pain perception remained comparable between groups (p > 0.05). Surgeons reported 93.33% satisfaction with NPA-assisted cases versus 33.33% with conventional methods (p < 0.001), correlating with fewer procedural interruptions. These findings establish NPA as a clinically superior modality for pediatric dental sedation, offering dual benefits of improved oxygenation stability and procedural workflow efficiency. The technology addresses critical gaps in perioperative safety while maintaining hemodynamic equilibrium, positioning it as a viable optimization strategy for high-risk pediatric populations.","PeriodicalId":520677,"journal":{"name":"Journal of investigative medicine : the official publication of the American Federation for Clinical Research","volume":" ","pages":"10815589251348914"},"PeriodicalIF":0.0,"publicationDate":"2025-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144188789","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Osteoblasts inhibit NK cell killing function via RANK/RANKL in multiple myeloma. EXPRESS：成骨细胞通过RANK/RANKL抑制多发性骨髓瘤NK细胞杀伤功能。

Journal of investigative medicine : the official publication of the American Federation for Clinical Research Pub Date : 2025-05-30 DOI: 10.1177/10815589251348915

Nanhao Meng, Xueke Zhu, Nannan Xie, Zhiwei Wang, Hao Wang, Chun Yang, Zhenhua Pan, Zhaoyun Liu, Rong Fu

{"title":"Osteoblasts inhibit NK cell killing function via RANK/RANKL in multiple myeloma.","authors":"Nanhao Meng, Xueke Zhu, Nannan Xie, Zhiwei Wang, Hao Wang, Chun Yang, Zhenhua Pan, Zhaoyun Liu, Rong Fu","doi":"10.1177/10815589251348915","DOIUrl":"10.1177/10815589251348915","url":null,"abstract":"Multiple myeloma (MM) is an incurable hematological malignancy. The bone marrow immune microenvironment plays a crucial role in MM progression. Our previous studies have shown that natural killer (NK) cell function is depleted in the bone marrow microenvironment of patients with MM. Therefore, it is urgent to explore the mechanisms underlying NK cell depletion and identify potential therapeutic targets. In this study, we focused on the mechanisms and potential therapeutic targets of MM osteoblasts that promote bone marrow NK cell depletion. The receptor activator of NF-kappa B (RANK) expression in bone marrow NK cells of patients with MM was significantly higher than that in normal controls. Serum receptor activator of NF-kappa B ligand (RANKL) levels in patients with MM also significantly higher than those in normal controls. MM cells can induce RANK expression in NK cells. Moreover, it is osteoblasts-not bone marrow mesenchymal stem cells-that secrete more RANKL. Blocking RANKL with denosumab resulted in increased CD107a expression, decreased KIR3DL1 expression, and increased release of perforin and granzyme B in NK cells. In addition, apoptosis was significantly increased in MM cells. Bone marrow osteoblasts in patients with MM may inhibit NK cell function via RANK/RANKL. Furthermore, denosumab combined with lenalidomide or pomalidomide can improve bone marrow NK cell function in these patients.","PeriodicalId":520677,"journal":{"name":"Journal of investigative medicine : the official publication of the American Federation for Clinical Research","volume":" ","pages":"10815589251348915"},"PeriodicalIF":0.0,"publicationDate":"2025-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144188791","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Free androgen index and its relation with female pattern hair loss. 游离雄激素指数及其与女性型脱发的关系。

Journal of investigative medicine : the official publication of the American Federation for Clinical Research Pub Date : 2025-05-30 DOI: 10.1177/10815589251348920

Rania Hosny Mahmoud, Shaimaa Thabet, Mai Ashraf Hosni

{"title":"Free androgen index and its relation with female pattern hair loss.","authors":"Rania Hosny Mahmoud, Shaimaa Thabet, Mai Ashraf Hosni","doi":"10.1177/10815589251348920","DOIUrl":"10.1177/10815589251348920","url":null,"abstract":"Androgenetic alopecia (AGA) is more common in women than in men. This study aims to evaluate various androgenic hormones in women and to assess if free androgen index (FAI) can be used as a marker of hyperandrogenism in women. Forty women with AGA (defined as grade 1, 2, or 3) were taken as subjects, and 40 other healthy women were included as controls in the study. The serum concentrations of testosterone (T) and sex hormone-binding globulin (SHBG) were measured, and FAI was calculated accordingly and compared with the control group. Results revealed that there were significant differences in the mean value of the two indicators (testosterone and FAI) in cases as compared to the controls. While the level of SHBG tended to be higher in controls than AGA cases. FAI appeared to be a better biomarker to predict hyperandrogenism than testosterone and SHBG alone. It can be concluded that FAI could be considered a good marker for an individual's androgen and can be recommended to be a vital biomarker of AGA in females. It is recommended to apply these parameters in the routine investigations for female patients with AGA.","PeriodicalId":520677,"journal":{"name":"Journal of investigative medicine : the official publication of the American Federation for Clinical Research","volume":" ","pages":"10815589251348920"},"PeriodicalIF":0.0,"publicationDate":"2025-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144188788","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Understanding the correlation of IL-6 with circRNA-DLGAP4 in the pathogenesis and diagnosis of rheumatoid arthritis. EXPRESS：了解IL-6与CircRNA-DLGAP4在类风湿关节炎发病和诊断中的相关性。

Journal of investigative medicine : the official publication of the American Federation for Clinical Research Pub Date : 2025-05-30 DOI: 10.1177/10815589251348916

Laila Mahdi, Rehab Elsayed Marzouk, Olfat G Shaker, Yasmine M Amrousy, Rania Talaat, Mona Mahrous Abdelaty, Noha O Shawky, Mai A El Kosaier, Marwa Kame

{"title":"Understanding the correlation of IL-6 with circRNA-DLGAP4 in the pathogenesis and diagnosis of rheumatoid arthritis.","authors":"Laila Mahdi, Rehab Elsayed Marzouk, Olfat G Shaker, Yasmine M Amrousy, Rania Talaat, Mona Mahrous Abdelaty, Noha O Shawky, Mai A El Kosaier, Marwa Kame","doi":"10.1177/10815589251348916","DOIUrl":"10.1177/10815589251348916","url":null,"abstract":"Rheumatoid arthritis (RA) as a systemic autoimmune disease is often misdiagnosed in its early stages due to vague symptoms, delaying treatment and increasing the likelihood of severe complications including cardiovascular mortality. The main aim of this work is to evaluate the serum levels of interleukin 6 (IL-6) and circular RNA DLGAP4 (circRNA-DLGAP4) in RA patients and healthy controls, also analyzing the correlations between all biomarkers to indicate whether those can be used as predictors for early diagnoses of RA. One hundred subjects were included, they were divided into two equal groups, 50-healthy matched individuals and 50 RA patients. For all participants, a venous blood sample for serum isolation was taken and analyzed for the serum level of IL-6 and circRNA-DLGAP4 as well as laboratory tests. For all patients, full clinical investigations have been performed as well as Disease Activity Score (DAS28) and modified Larsen radiological scoring have been calculated. Results revealed that there was a significant up-regulation of the concentration level of IL-6 (p = 0.0001) and circRNA-DLGAP4 expression (p = 0.0001) in the RA group as compared to healthy controls. Moreover, altered expression of circRNA-DLGAP4 showed a slight significant difference between patients with positive deformities with p = 0.05. Serum levels of both IL-6 and circRNA-DLGAP4 increased with the severity of RA. Various correlations were detected of both IL-6 and circRNA-DLGAP4 with the associated RA parameters. It can be concluded that IL-6 and circRNA-DLGAP4 could have a potential role in RA pathogenesis and could act as possible biomarkers of RA for further studies to be applied for RA diagnostic purposes.","PeriodicalId":520677,"journal":{"name":"Journal of investigative medicine : the official publication of the American Federation for Clinical Research","volume":" ","pages":"10815589251348916"},"PeriodicalIF":0.0,"publicationDate":"2025-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144188793","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Advanced therapies for stomach cancer. EXPRESS：胃癌的先进疗法。

Journal of investigative medicine : the official publication of the American Federation for Clinical Research Pub Date : 2025-05-30 DOI: 10.1177/10815589251348919

Dong Luo, Yunmei Liu, Lei Huang

{"title":"Advanced therapies for stomach cancer.","authors":"Dong Luo, Yunmei Liu, Lei Huang","doi":"10.1177/10815589251348919","DOIUrl":"10.1177/10815589251348919","url":null,"abstract":"Gastric cancer (GC) persists as a major global health challenge, with advanced-stage disease exhibiting persistently poor prognoses despite advancements in early diagnosis and conventional treatments. This clinical urgency has driven the adoption of advanced therapies, including molecularly targeted therapies and immunotherapeutic strategies. Human epidermal growth factor receptor 2 (HER2)-directed agents, including trastuzumab, trastuzumab deruxtecan (T-DXd), and disitamab vedotin (RC48), have demonstrated significant survival benefits in HER2-positive cohorts, though intratumoral heterogeneity frequently underlies acquired resistance. Antiangiogenic therapies targeting the vascular endothelial growth factor receptor, exemplified by ramucirumab, remain a cornerstone of advanced GC management. Concurrently, immune checkpoint inhibitors against programmed cell death protein 1 and programmed cell death-ligand 1 have expanded therapeutic options by potentiating endogenous antitumor immunity, albeit with variable efficacy across molecular subtypes. Emerging immunotherapies-such as adoptive cell therapies (e.g., chimeric antigen receptor T-cells), tumor-associated antigen vaccines, immunomodulatory agents, and genetically engineered oncolytic viruses-show promising preclinical and early phase clinical activity. Critical to optimizing these advances is a systems-level understanding of the tumor-immune microenvironment, which dynamically regulates therapeutic response and immune evasion. Future progress hinges on three pillars: (1) biomarker-driven personalization of treatment regimens, (2) combinatorial strategies to overcome primary and adaptive resistance mechanisms, and (3) translational integration of multi-omics insights into therapeutic development. Addressing these priorities through mechanistic investigations and innovative trial designs will be essential to achieving durable clinical responses and improving survival outcomes in this heterogeneous malignancy.","PeriodicalId":520677,"journal":{"name":"Journal of investigative medicine : the official publication of the American Federation for Clinical Research","volume":" ","pages":"10815589251348919"},"PeriodicalIF":0.0,"publicationDate":"2025-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144192658","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0