Journal of investigative medicine : the official publication of the American Federation for Clinical Research最新文献

{"title":"EXPRESS: Two Decades of Inflammatory Bowel Disease-Related Cardiovascular Mortality in the United States: Temporal Trends and Demographic Disparities from a National Death Registry.","authors":"Abdalhakim Shubietah, Mohammad Bdair, Anwar Zahran, Mohammed AbuBaha, Maysam Tawba, Abubakar Nazir, Mohamed S Elgendy, Ameer Awashra, Mohammed Tareq Mutar, Jehad Zeidalkilani, Hosam I Taha, Fathi Milhem, Ahmed Emara, Suleiman Khreshi, Abdallah Hussein","doi":"10.1177/10815589251366911","DOIUrl":"https://doi.org/10.1177/10815589251366911","url":null,"abstract":"<p><p>Although the association between inflammatory bowel disease (IBD) and cardiovascular disease (CVD) is well established, long-term, population-level trends in cardiovascular mortality among individuals with IBD remain poorly characterized. This study provides a comprehensive, nationwide analysis of IBD-associated cardiovascular mortality in the United States over a two-decade period, emphasizing temporal patterns and sociodemographic disparities. We performed a retrospective analysis using the CDC WONDER database from 1999 to 2020, identifying decedents with CVD (ICD-10 I00-I99) as the underlying cause and IBD (ICD-10 K50/K51) as a contributing condition. Mortality rates were age-adjusted using the direct standardization method and stratified by demographic and geographic variables. Total of 11,891 cardiovascular deaths were linked to IBD during the study period. From 1999 to 2015, age-adjusted mortality rates (AAMRs) declined significantly, with an annual percent change of -2.74% (p = 0.0064). However, between 2015 and 2020, this trend plateaued. Subgroup analyses revealed significant post-2015 increases among Black individuals (+23.03%, 2017-2020; p = 0.0416) and men (+27.19%, 2018-2020; p = 0.0100). AAMRs were highest among White and non-Hispanic populations and varied by state and urbanization level. In addition, we implemented ARIMA-based forecasting models, which project continued increases in mortality among males and White individuals through 2040, suggesting that recent adverse trends may persist. Over the past two decades, IBD-related cardiovascular mortality in the U.S. declined until 2015, followed by stabilization and modest resurgence, particularly among men and Black individuals. These findings underscore persistent disparities and call for targeted, multidisciplinary strategies to mitigate cardiovascular risk among individuals with IBD.</p>","PeriodicalId":520677,"journal":{"name":"Journal of investigative medicine : the official publication of the American Federation for Clinical Research","volume":" ","pages":"10815589251366911"},"PeriodicalIF":2.0,"publicationDate":"2025-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144786441","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"EXPRESS: Liquid Biopsy in Breast Cancer Management: From Circulating Biomarkers to Clinical Breakthroughs.","authors":"Anmar Ghanim Taki, Abdulkareem Shareef, Lalji Baldaniya, Rami Oweis, S Renuka Jyothi, Udaybir Singh, Samir Sahoo, Ashish Singh Chauhan, Alisher Khazratov, Hayder Naji Sameer, Ahmed Yaseen, Zainab H Athab, Mohaned Adil","doi":"10.1177/10815589251366905","DOIUrl":"https://doi.org/10.1177/10815589251366905","url":null,"abstract":"<p><p>Breast cancer continues to be a major global health concern, particularly for women, despite improvements in early detection and treatment strategies. Traditional tissue biopsies have long been the foundation of diagnosis and treatment planning; however, they come with limitations-such as being invasive, providing only a single snapshot of a dynamic disease, and often missing the evolving molecular landscape, especially in metastatic cases. In recent years, liquid biopsy has emerged as a powerful, non-invasive tool offering real-time insights into tumor biology. By analyzing materials shed by tumors into the bloodstream-like circulating tumor DNA (ctDNA), circulating tumor cells (CTCs), exosomes, and cell-free RNA (cfRNA)-liquid biopsy can reveal changes in tumor burden, treatment response, and the appearance of drug resistance. These advantages make it especially valuable in personalizing care for breast cancer patients across various subtypes, including hormone receptor-positive, HER2-positive, and triple-negative breast cancer. This review highlights the components and technological platforms that underpin liquid biopsy, explores its current clinical applications, and discusses future directions, including integration with artificial intelligence and multi-omics analysis. As research advances and clinical evidence grows, liquid biopsy holds the promise of reshaping breast cancer care, making it more precise, timely, and individualized.</p>","PeriodicalId":520677,"journal":{"name":"Journal of investigative medicine : the official publication of the American Federation for Clinical Research","volume":" ","pages":"10815589251366905"},"PeriodicalIF":2.0,"publicationDate":"2025-08-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144769488","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"EXPRESS: Bispecific T-cell Engagers for Relapsed/Refractory Multiple Myeloma After Solid Organ Transplantation: A Case Series.","authors":"Raul Gregg Garcia, Rafat Abonour, Attaya Suvannasankha","doi":"10.1177/10815589251364807","DOIUrl":"https://doi.org/10.1177/10815589251364807","url":null,"abstract":"<p><p>The emergence of bispecific antibodies for multiple myeloma presents a critical unanswered question for solid organ transplant recipients: Can these T-cell dependent therapies overcome concurrent immunosuppression without triggering graft rejection? While teclistamab and similar agents demonstrate remarkable efficacy in immunocompetent patients, their mechanism demands functional T-cells - precisely what calcineurin inhibitors and antimetabolites suppress in transplant recipients. This creates a perilous therapeutic trilemma: (1) maintaining standard immunosuppression risks blunting bispecific efficacy, (2) reducing immunosuppression may precipitate graft rejection, while (3) both strategies compound already elevated infection risks from dual immunosuppressive effects. Our experience treating two renal transplant recipients with teclistamab reveals these tensions in practice - one patient achieved sustained response without rejection, while another faced infectious complications preceding disease progression. These cases highlight three urgent research priorities: prospective studies to define (a) minimum effective immunosuppression levels during bispecific therapy, (b) biomarkers predicting graft rejection risk, and (c) optimal infection prophylaxis strategies. Until such data exists, clinicians navigate this high-stakes balancing act without evidence, forced to weigh theoretical risks of allograft loss against known mortality from progressive myeloma.</p>","PeriodicalId":520677,"journal":{"name":"Journal of investigative medicine : the official publication of the American Federation for Clinical Research","volume":" ","pages":"10815589251364807"},"PeriodicalIF":2.0,"publicationDate":"2025-07-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144736564","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"EXPRESS: Independent writing versus ChatGPT-generated manuscript followed by extensive human editing: The products become similar?","authors":"Shigeki Matsubara","doi":"10.1177/10815589251363634","DOIUrl":"https://doi.org/10.1177/10815589251363634","url":null,"abstract":"<p><p>Not needed.</p>","PeriodicalId":520677,"journal":{"name":"Journal of investigative medicine : the official publication of the American Federation for Clinical Research","volume":" ","pages":"10815589251363634"},"PeriodicalIF":0.0,"publicationDate":"2025-07-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144669273","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"EXPRESS: Changes in mucin expression in the lungs of infant piglets following cardiopulmonary bypass with deep hypothermic circulatory arrest.","authors":"Ludmila Khailova, Justin Robison, Richard Ing, Suzanne Lujan-Osorio, Jesse Davidson","doi":"10.1177/10815589251363491","DOIUrl":"https://doi.org/10.1177/10815589251363491","url":null,"abstract":"<p><p>Atelectasis and mucus plugging are common complications following pediatric cardiac surgery with cardiopulmonary bypass (CPB). Mucins increase in airway lavage after pediatric CPB, however, tissue expression and regional variation remain to be evaluated in a translational model.The objective of this study was to analyze mucins in the lungs of infant pigs undergoing CPB with deep hypothermic circulatory arrest (DHCA).Piglets underwent CPB with DHCA followed by survival for 4h (n=5). Anesthesia controls (n=7) were mechanically ventilated for 7h without CPB. Gene expression of Muc4, Muc5AC and Muc5B in the right apex (RA) and right lower lobe (RLL) was analyzed using RT-PCR and localization and production were evaluated by immunohistochemistry.CPB/DHCA animals had significantly increased Muc4 (26.7±30.6 vs 1.2±1.2; p=0.017), Muc5AC (7.8±8.6 vs 1.1±1.0; p=0.0025), and Muc5B (8.1±8.9 vs 1.5±1.4; p=0.017) mRNA levels and higher number of Muc5B positive cells in submucosal glands and bronchiole surface of the RLL compared to controls, with similar trends in the RA. Muc5AC localized mainly to bronchiole goblet cells, and its production qualitatively increased in the lungs of CPB/DHCA animals.Tissue mucin expression and production increases in the lungs of piglets exposed to CPB/DHCA, particularly in the lower lobes. Thus, the risk of mucus plugging in the acute period may be somewhat higher in the lower lobes, which are already affected by atelectasis and inflammation. Our model may serve as a valuable platform for future studies evaluating the mechanism and therapy of mucin production and airway obstruction following pediatric cardiac surgery.</p>","PeriodicalId":520677,"journal":{"name":"Journal of investigative medicine : the official publication of the American Federation for Clinical Research","volume":" ","pages":"10815589251363491"},"PeriodicalIF":0.0,"publicationDate":"2025-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144669272","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"EXPRESS: PREVALENCE AND PREDICTORS OF AUTOIMMUNE GASTRITIS IN PATIENTS WITH CELIAC DISEASE.","authors":"Ramazan Erdem Er, Volkan Yilmaz, Yigit Baykara, Fatih Acehan, Cagdas Kalkan, Irfan Soykan","doi":"10.1177/10815589251361891","DOIUrl":"https://doi.org/10.1177/10815589251361891","url":null,"abstract":"<p><p>Celiac disease is an immune-mediated disease triggered by gluten in genetically susceptible individuals. Autoimmune gastritis is an autoimmune disease of the stomach characterized by autoantibodies directed against some structures containing H+/K+-ATPase and intrinsic factor. Both diseases may lead to micronutrient malabsorption and anemia. The goals of this study were to investigate the prevalence of autoimmune gastritis in patients with celiac disease and determine the possible factors that might predict presence of autoimmune gastritis in patients with celiac disease. Patients diagnosed with celiac disease were investigated and data were collected regarding patient demographics, presence of concomitant autoimmune diseases, serological markers and micronutrient levels. Diagnosis of celiac disease has been established according to the Marsh classification. Autoimmune gastritis was diagnosed according to histopathologic findings. 183 patients with eliac disease were enrolled. Out of 183 patients, there were 19 patients with autoimmune gastritis (10.4%). In multivariable regression analysis, Marsh type 2 celiac disease (OR: 15.838; 95% CI: 4.774-52.545) and the absence of anti-endomysium IgA (OR: 0.257; 95% CI: 0.084-0.786) were identified as predictors of autoimmune gastritis in patients with celiac disease. 10.4% of patients with celiac disease also had autoimmune gastritis. Factors that might predict the presence of a concomitant AIG were the presence of Marsh type 2 celiac disease and absence of anti-endomysium IgA in patients with celiac disease. Therefore, we suggest that patients negative for anti-endomysium IgA antibody and who have Marsh type 2 celiac disease should be screened for the presence of autoimmune gastritis.</p>","PeriodicalId":520677,"journal":{"name":"Journal of investigative medicine : the official publication of the American Federation for Clinical Research","volume":" ","pages":"10815589251361891"},"PeriodicalIF":0.0,"publicationDate":"2025-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144639574","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"EXPRESS: Potential risk genotypes associated with symptoms of pesticide poisoning based on polymorphisms in the XRCC1 (rs25487 and rs1799782), SOD2 (rs4880), PON1 (rs662) and GSTP1 (rs1695) genes, in farmers in the Cienega region.","authors":"Carlos Alfonso Flores-Gutierrez, Erandis Dheni Torres-Sanchez, Emmanuel Reyes-Uribe, Iran Cotes-Trujillo, Gabriela Martinez-Cortes, Marco Antonio Hernandez-Bedolla, Joel Salazar-Flores","doi":"10.1177/10815589251361367","DOIUrl":"https://doi.org/10.1177/10815589251361367","url":null,"abstract":"<p><p>The variants in the genes involved in the defense mechanisms against pesticide poisoning could be the cause to develop symptoms of poisoning with these substances. This work, we analyses polymorphisms in genes that defend against pesticide poisoning in 112 farmers from the Cienega region of Jalisco. We genotyped the variants rs25487 and rs1799782 of the XRCC1 gene, rs28360135 of the XRCC4 gene, rs4880 of the SOD2 gene, rs662 and rs1695 of the PON1 and GSTP1 genes, respectively. For rs25487 (AA vs AG; p = 0.045) significance was found with heart palpitations, rs1799782 with salivation (CC vs CT; p = 0.038), rs4880 with sweating (TT+TC vs CC; p = 0.026) (TC vs CC; p = 0.034), rs662 with cough (AA vs AG + GG; p = 0.001) (AA vs AG; p = 0.006) (AA vs GG; p = 0.027) and salivation (AG vs GG; p = 0.037). Furthermore, the variant rs1799782 found significance with carbamates (p = 0.049) and pyrethroids (p = 0.015), as well as the TT vs TC+CC (p = 0.043) and TT vs TC (p = 0.034) genotypes of rs4880 with carbamates. Finally, AA+AG vs GG (p = 0.039) and AG vs GG (p = 0.025) genotypes of the rs1695 polymorphism were also significant with carbamates. Except for the XRCC4 rs28360135 and GSTP1 rs1695 variants, the rest of the polymorphisms showed significance with symptoms of acute poisoning. The presence of XRCC1 (rs1799782), SOD2 (rs4880) and GSTP1 (rs1695) variants may be risk factors for carbamate and pyrethroid poisoning.</p>","PeriodicalId":520677,"journal":{"name":"Journal of investigative medicine : the official publication of the American Federation for Clinical Research","volume":" ","pages":"10815589251361367"},"PeriodicalIF":0.0,"publicationDate":"2025-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144629436","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"EXPRESS: BMP6 Contributes As a Blood-Based Biomarker to Clinical Discrimination of Patients with Carotid Artery Stenosis: Decoding the Transcriptome of Plaque Biology.","authors":"Atif Yolgosteren, Ceyda Colakoglu Bergel, Unal Egeli, Isil Ezgi Eryilmaz, Can Kosukcu, Guven Ozkaya, Basak Erdemli Gursel, Murat Bicer, Gulsah Cecener, Efe Ozoglu, Emre Sarandol, Mustafa Tok","doi":"10.1177/10815589251361364","DOIUrl":"https://doi.org/10.1177/10815589251361364","url":null,"abstract":"<p><p>Carotid artery stenosis (CAS) patients are classified as \"symptomatic\" (SCAS) and \"asymptomatic\" (ACAS) based on their symptoms. For SCAS and ACAS, complementary biomarkers are needed since the degree of stenosis is insufficient for selecting a treatment or risk assessment. Carotid plaque ulceration represents vulnerable features, and the symptomatic plaque is considered vulnerable. The current study aimed to investigate a blood-based biomarker to determine the presence of ulceration/symptoms in blood by analyzing plaque material based on surface morphology. RNA-Sequencing (RNA-Seq) was performed using the Ion AmpliSeq Transcriptome Human Gene Expression Panel. Additionally, bioinformatic analysis was performed using IPA. Then, gene expression and protein levels were analyzed using RT-qPCR and ELISA. Based on IPA, immune responses, and ROS production, lipid transport is activated in SCAS patients. For ulcerated and smooth plaque comparison, it was determined that lipid and sterol transport, and catabolism of lipids, occurred in ulcerated plaque. Based on RT-qPCR, while TIMP3, DES, ITLN, CHI3L2, FHL5, CLU, and FIBIN genes were statistically significant in plaque material, CCL24, BMP6, and IL6 were also significant in blood. Interestingly, these genes were downregulated in both plaque and blood. For ulcerated plaque, CCL24 and BMP6 were regulated in both blood and plaque, but only BMP6 was determined to be downregulated in both. Finally, multivariate binary logistic regression shows that BMP6 had the most remarkable ability to predict the SCAS vs. ACAS discrimination ([OR]=0.553, p=0.019). Although more studies are warranted, our results suggest that BMP6 seems to be a blood-based biomarker for discriminating SCAS vs. ACAS.</p>","PeriodicalId":520677,"journal":{"name":"Journal of investigative medicine : the official publication of the American Federation for Clinical Research","volume":" ","pages":"10815589251361364"},"PeriodicalIF":0.0,"publicationDate":"2025-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144629434","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"EXPRESS: Differential oxidative stress profiles in nephrolithiasis and ureterolithiasis: biochemical insights from serum and urinary markers.","authors":"Marek Biesiadecki, Krzysztof Balawender, Mateusz Mołoń, Agnieszka Mołoń, Sabina Galiniak","doi":"10.1177/10815589251361161","DOIUrl":"https://doi.org/10.1177/10815589251361161","url":null,"abstract":"<p><p>Urolithiasis, characterized by the formation of stones within the urinary tract, poses a significant medical challenge influenced by multifactorial origins including genetic predisposition, diet, and lifestyle factors. This condition predominantly affects males and exhibits varying prevalence rates across different global regions. Oxidative disturbances have been implicated in urolithiasis pathogenesis through mechanisms involving renal inflammation and tissue damage. This study investigated serum and urinary redox markers in 107 patients with kidney or ureteral stones versus 80 healthy controls. To the best of our knowledge, this represents the initial investigation comparing oxidative stress levels in the serum and urine of patients afflicted with nephrolithiasis and ureterolithiasis. We observed significantly elevated levels of advanced oxidation protein products (AOPP, p<0.01), malondialdehyde (MDA, p<0.001), total oxidant status (TOS, p<0.001), and oxidative stress index (OSI, p<0.001) in serum of urolithiasis patients, alongside decreased total antioxidant capacity (TAC, p<0.001). There were no differences in thiol group and Amadori products concentrations between studied groups. Moreover, increased concentration of AOPP (p<0.001), TOS (p<0.01) and OSI (p<0.01) as well as decreased level of TAC (p<0.001) was observed in urine of urolithiasis patients. Distinct patterns of oxidative stress markers were observed between nephrolithiasis and ureterolithiasis subgroups, underscoring differential oxidative stress profiles in these conditions. These findings may support early diagnosis, risk stratification, and personalized management of urolithiasis patients by revealing distinct oxidative stress profiles associated with stone location (renal vs ureteral).</p>","PeriodicalId":520677,"journal":{"name":"Journal of investigative medicine : the official publication of the American Federation for Clinical Research","volume":" ","pages":"10815589251361161"},"PeriodicalIF":0.0,"publicationDate":"2025-07-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144629435","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Letter to the Editor regarding EXPRESS: Large language model content detectors: Effects of human editing and ChatGPT mimicking individual style: A preliminary study.","authors":"Amnuay Kleebayoon, Viroj Wiwanitkit","doi":"10.1177/10815589251360324","DOIUrl":"10.1177/10815589251360324","url":null,"abstract":"","PeriodicalId":520677,"journal":{"name":"Journal of investigative medicine : the official publication of the American Federation for Clinical Research","volume":" ","pages":"10815589251360324"},"PeriodicalIF":0.0,"publicationDate":"2025-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144586189","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}